CA3175780A1 - Terminal deoxynucleotidyl transferase variants and uses thereof - Google Patents

Terminal deoxynucleotidyl transferase variants and uses thereof Download PDF

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Publication number
CA3175780A1
CA3175780A1 CA3175780A CA3175780A CA3175780A1 CA 3175780 A1 CA3175780 A1 CA 3175780A1 CA 3175780 A CA3175780 A CA 3175780A CA 3175780 A CA3175780 A CA 3175780A CA 3175780 A1 CA3175780 A1 CA 3175780A1
Authority
CA
Canada
Prior art keywords
amino acid
seq
substituted
respect
acid position
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3175780A
Other languages
English (en)
French (fr)
Inventor
Mikhael SOSKINE
Elise Champion
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DNA Script SAS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA3175780A1 publication Critical patent/CA3175780A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3175780A 2020-04-20 2021-04-16 Terminal deoxynucleotidyl transferase variants and uses thereof Pending CA3175780A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170405 2020-04-20
EP20170405.3 2020-04-20
PCT/EP2021/059861 WO2021213903A1 (en) 2020-04-20 2021-04-16 Terminal deoxynucleotidyl transferase variants and uses thereof

Publications (1)

Publication Number Publication Date
CA3175780A1 true CA3175780A1 (en) 2021-10-28

Family

ID=70390759

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3175780A Pending CA3175780A1 (en) 2020-04-20 2021-04-16 Terminal deoxynucleotidyl transferase variants and uses thereof

Country Status (9)

Country Link
US (1) US20230159903A1 (https=)
EP (1) EP4139446A1 (https=)
JP (1) JP2023522234A (https=)
KR (1) KR20230002825A (https=)
CN (1) CN115427559A (https=)
AU (1) AU2021260014A1 (https=)
CA (1) CA3175780A1 (https=)
IL (1) IL297425A (https=)
WO (1) WO2021213903A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021058438A1 (en) 2019-09-23 2021-04-01 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
CA3234383A1 (en) 2021-10-15 2023-04-27 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
WO2023187214A1 (en) 2022-03-31 2023-10-05 Ribbon Biolabs Gmbh Biopolymer synthesis
EP4735621A1 (en) 2023-06-30 2026-05-06 DNA Script Nucleic acid synthesis on reusable support
GB2635104A (en) * 2023-09-08 2025-05-07 Evonetix Ltd Enzymes and methods
WO2025088024A1 (en) * 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
US20250313876A1 (en) 2024-04-06 2025-10-09 Ribbon Bio GmbH Isolation of dna fragments
WO2026008711A2 (en) 2024-07-02 2026-01-08 Dna Script Filtering device and method for polynucleotide enzymatic synthesis
WO2026008717A2 (en) 2024-07-02 2026-01-08 Dna Script Method for enzymatic synthesis of polynucleotides
WO2026017834A1 (en) 2024-07-19 2026-01-22 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis and cleavage

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Publication number Priority date Publication date Assignee Title
US5367066A (en) 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
AU1436995A (en) 1993-12-30 1995-07-17 Chemgenes Corporation Synthesis of propargyl modified nucleosides and phosphoramidites and their incorporation into defined sequence oligonucleotides
EP0776330B1 (en) 1994-06-23 2003-08-20 Affymax Technologies N.V. Photolabile compounds and methods for their use
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
EP0967217B1 (en) 1998-06-22 2005-12-21 Affymetrix, Inc. (a California Corporation) Reagents and methods for solid phase synthesis and display
ATE296310T1 (de) 1999-03-08 2005-06-15 Metrigen Inc Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
US6664097B2 (en) 2000-05-23 2003-12-16 North Carolina State University Polynucleotide encoding a Lactobacillus gasseri beta-glucuronidase polypeptide
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20060086538A1 (en) 2002-07-08 2006-04-27 Shell Oil Company Choke for controlling the flow of drilling mud
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US8212020B2 (en) 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
US7544794B1 (en) 2005-03-11 2009-06-09 Steven Albert Benner Method for sequencing DNA and RNA by synthesis
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
US8034923B1 (en) 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
EP2876166B1 (en) 2013-11-20 2016-12-14 Roche Diagnostics GmbH New compound for sequencing by synthesis
FR3020071B1 (fr) * 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
GB201502152D0 (en) * 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
FR3052462A1 (fr) 2016-06-14 2017-12-15 Dna Script Variants d'une adn polymerase de la famille polx
US10472383B2 (en) 2017-03-16 2019-11-12 Steven A Benner Nucleoside triphosphates with stable aminoxy groups
AU2018272302A1 (en) * 2017-05-26 2019-12-19 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
US20190078065A1 (en) 2017-09-08 2019-03-14 Sigma-Aldrich Co. Llc Modified dna polymerases
US10435676B2 (en) * 2018-01-08 2019-10-08 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10752887B2 (en) * 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
CA3119845A1 (en) * 2018-11-14 2020-05-22 Dna Script Terminal deoxynucleotidyl transferase variants and uses thereof
SG11202105441WA (en) * 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
WO2020165137A1 (en) * 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.

Also Published As

Publication number Publication date
EP4139446A1 (en) 2023-03-01
JP2023522234A (ja) 2023-05-29
CN115427559A (zh) 2022-12-02
AU2021260014A1 (en) 2022-11-17
IL297425A (en) 2022-12-01
KR20230002825A (ko) 2023-01-05
WO2021213903A1 (en) 2021-10-28
US20230159903A1 (en) 2023-05-25

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