JP2023511527A - Ｈｍｏの産生 - Google Patents

Ｈｍｏの産生 Download PDF

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Publication number
JP2023511527A
JP2023511527A JP2022540729A JP2022540729A JP2023511527A JP 2023511527 A JP2023511527 A JP 2023511527A JP 2022540729 A JP2022540729 A JP 2022540729A JP 2022540729 A JP2022540729 A JP 2022540729A JP 2023511527 A JP2023511527 A JP 2023511527A
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JP
Japan
Prior art keywords
nucleic acid
seq
gene
hmos
lacto
Prior art date
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Pending
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JP2022540729A
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English (en)
Japanese (ja)
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JP2023511527A5 (https=
Inventor
マルギット ペダーセン，
マノス パパダキス，
カトリーヌ， ビッチ カンプマン，
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Glycom AS
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Glycom AS
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Publication date
Application filed by Glycom AS filed Critical Glycom AS
Publication of JP2023511527A publication Critical patent/JP2023511527A/ja
Publication of JP2023511527A5 publication Critical patent/JP2023511527A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01065—3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01146—Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase (2.4.1.146)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2022540729A 2020-01-23 2021-01-22 Ｈｍｏの産生 Pending JP2023511527A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA202000085 2020-01-23
DKPA202000085 2020-01-23
DKPA202000833 2020-07-13
DKPA202000833 2020-07-13
PCT/EP2021/051473 WO2021148615A1 (en) 2020-01-23 2021-01-22 Hmo production

Publications (2)

Publication Number Publication Date
JP2023511527A true JP2023511527A (ja) 2023-03-20
JP2023511527A5 JP2023511527A5 (https=) 2023-09-22

Family

ID=74556858

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022540729A Pending JP2023511527A (ja) 2020-01-23 2021-01-22 Ｈｍｏの産生

Country Status (7)

Country Link
US (1) US20230193335A1 (https=)
EP (1) EP4093749A1 (https=)
JP (1) JP2023511527A (https=)
CN (1) CN114981290B (https=)
AU (1) AU2021209394A1 (https=)
BR (1) BR112022014414A2 (https=)
WO (1) WO2021148615A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025047865A1 (ja) * 2023-08-30 2025-03-06 キリンホールディングス株式会社 遺伝子改変微生物、及びオリゴ糖の製造方法
WO2025047866A1 (ja) * 2023-08-30 2025-03-06 キリンホールディングス株式会社 遺伝子改変微生物、及びオリゴ糖の製造方法

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK180952B1 (en) * 2020-12-22 2022-08-10 Glycom As A dfl-producing strain
EP4281564A1 (en) 2021-01-22 2023-11-29 Glycom A/S New major facilitator superfamily (mfs) protein (fred) in production of sialylated hmos
DK181497B1 (en) * 2021-05-17 2024-03-12 Dsm Ip Assets Bv ENHANCING FORMATION OF THE HMOS LNT AND/OR LNnT BY MODIFYING LACTOSE IMPORT IN THE CELL
DK181991B1 (en) 2021-11-11 2025-05-15 Dsm Ip Assets Bv Combined fermentation process for producing one or more human milk oligosaccharide(s) (hmo(s)) in escherichia coli
DK181683B1 (en) 2022-03-02 2024-09-20 Dsm Ip Assets Bv Cells exprssing new sialyltransferases for in vivo synthesis of lst-a, methods using same and constructs encoding said sialyltransferases
CN118786221A (zh) 2022-03-02 2024-10-15 帝斯曼知识产权资产管理有限公司 用于体内合成3'sl和6'sl的新型唾液酸转移酶
DK181319B1 (en) 2022-03-02 2023-08-10 Dsm Ip Assets Bv Genetically engineered cells and methods comprising use of a sialyltransferase for in vivo synthesis of 3’sl
EP4514989A1 (en) 2022-04-29 2025-03-05 DSM IP Assets B.V. Hmo producing microorganism with increased robustness towards glucose gradients
DK182075B1 (en) * 2022-06-13 2025-07-02 Dsm Ip Assets Bv Genetically engineered cells comprising sigma factor modifications for production of heterologous products and methods for use of same
DK181765B1 (en) 2022-07-15 2024-12-04 Dsm Ip Assets Bv Cells expressing new fucosyltransferases for in vivo synthesis of lnfp-iii, and methods and uses of same
DK181911B1 (en) * 2022-07-15 2025-03-18 Dsm Ip Assets Bv GENETICALLY ENGINEERED CELLS COMPRISING A RECOMBINANT NUCLEIC ACID SEQUNCE ENCODING A FUCOSYLTRANSFERASE FOR IN VIVO SYNTHESIS OF COMPLEX FUCOSYLATED HUMAN MILK OLIGOSACCHARIDES (HMOs) AND METHODS FOR PRODUCING THE HMOs AND USE OF THE ENZYME
EP4577061A1 (en) 2022-08-25 2025-07-02 DSM IP Assets B.V. Hybrid method for producing complex hmos
CN120265786A (zh) 2022-11-25 2025-07-04 帝斯曼知识产权资产管理有限公司 用于生产寡糖的双菌株系统
DK182185B1 (en) * 2022-12-14 2025-10-29 Dsm Ip Assets Bv Mutated lacto-n-biosidase,method for making the same and method for synthesizing lacto-n-biose containing carbohydrates
CN117867054B (zh) * 2023-01-09 2024-09-27 山东恒鲁生物科技有限公司 合成hmo的方法
CN120677242A (zh) 2023-02-24 2025-09-19 帝斯曼知识产权资产管理有限公司 用于体内合成人乳寡糖的产物特异性转运蛋白
DK181822B1 (en) 2023-06-07 2025-01-30 Dsm Ip Assets Bv Genetically engineered strains with reduced byproduct formation and methods and uses of same
DK182227B1 (en) 2023-10-17 2025-12-18 Dsm Ip Assets Bv Genetically engineered cells comprising new fucosyltransferases for in vivo synthesis of complex fucosylated human milk oligosaccharides mixtures comprising lnfp-vi, methods using the same, and uses of new fucosyltransferases thereof.
WO2025190861A1 (en) 2024-03-11 2025-09-18 Dsm Ip Assets B.V. Fucosyltransferase mutants with low dfl formation
DK202430472A1 (en) 2024-08-19 2026-02-24 Dsm Ip Assets Bv Alpha-1,2 fucosyltransferase mutants with increased alpha 1,3-fucosyltransferase activity

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018526019A (ja) * 2015-09-12 2018-09-13 イェネヴァイン ビオテヒノロギー ゲーエムベーハー 取り込み／排出を改変した微生物宿主におけるヒトミルクオリゴ糖の生産

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2440661T3 (en) 2009-06-08 2018-03-12 Jennewein Biotechnologie Gmbh HMO synthesis
DE12746649T1 (de) 2011-02-16 2016-09-29 Glycosyn LLC Biosynthese menschlicher milcholigosaccaride in manipulierten Bakterien
WO2015188834A1 (en) 2014-06-11 2015-12-17 Glycom A/S Separation of 2'-o-fucosyllactose from fermentation broth
AU2015315110B2 (en) 2014-09-09 2021-04-08 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides
DK3233875T3 (en) 2014-12-16 2022-12-19 Glycom As Separation of 2'-fl from a fermentation broth
ES2991621T3 (es) 2016-03-07 2024-12-04 Glycom As Separación de oligosacáridos de caldo de fermentación
DK3445770T3 (da) 2016-04-19 2026-02-23 Glycom As Separering af oligosaccharider fra fermenteringssuppe
ES2856749T3 (es) 2016-10-29 2021-09-28 Chr Hansen Hmo Gmbh Proceso para la producción de oligosacáridos fucosilados
CN111727253B (zh) 2017-12-21 2024-08-27 格礼卡姆股份公司 用于体外和体内基因表达的核酸构建体
US20190323052A1 (en) 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing export of 2? fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
US20190323053A1 (en) 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing activity of 2? fucosyllactose transporters endogenous to microbial cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018526019A (ja) * 2015-09-12 2018-09-13 イェネヴァイン ビオテヒノロギー ゲーエムベーハー 取り込み／排出を改変した微生物宿主におけるヒトミルクオリゴ糖の生産

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"ACCESSION; SEQ18517, DEFINITION; MFS transporter, SET family, sugar efflux transporter [Rosenbergiel", DATABASE GENBANK [ONLINE], JPN6024034979, 29 October 2016 (2016-10-29), ISSN: 0005407751 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025047865A1 (ja) * 2023-08-30 2025-03-06 キリンホールディングス株式会社 遺伝子改変微生物、及びオリゴ糖の製造方法
WO2025047866A1 (ja) * 2023-08-30 2025-03-06 キリンホールディングス株式会社 遺伝子改変微生物、及びオリゴ糖の製造方法

Also Published As

Publication number Publication date
CN114981290A (zh) 2022-08-30
CN114981290B (zh) 2024-12-06
EP4093749A1 (en) 2022-11-30
AU2021209394A1 (en) 2022-07-14
US20230193335A1 (en) 2023-06-22
BR112022014414A2 (pt) 2022-09-13
WO2021148615A1 (en) 2021-07-29

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