JP2023046320A5 - - Google Patents
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- JP2023046320A5 JP2023046320A5 JP2022150677A JP2022150677A JP2023046320A5 JP 2023046320 A5 JP2023046320 A5 JP 2023046320A5 JP 2022150677 A JP2022150677 A JP 2022150677A JP 2022150677 A JP2022150677 A JP 2022150677A JP 2023046320 A5 JP2023046320 A5 JP 2023046320A5
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- JP
- Japan
- Prior art keywords
- gene
- filamentous fungus
- biomass
- pgab
- peld
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108090000623 proteins and genes Proteins 0.000 claims 19
- 241000233866 Fungi Species 0.000 claims 13
- 238000000034 method Methods 0.000 claims 11
- 239000002028 Biomass Substances 0.000 claims 6
- 108010029182 Pectin lyase Proteins 0.000 claims 3
- 108010059820 Polygalacturonase Proteins 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 3
- 241000228245 Aspergillus niger Species 0.000 claims 2
- 108010059892 Cellulase Proteins 0.000 claims 2
- 241000223259 Trichoderma Species 0.000 claims 2
- 241000499912 Trichoderma reesei Species 0.000 claims 2
- 229940106157 cellulase Drugs 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims 1
- 241000658379 Manihot esculenta subsp. esculenta Species 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000001814 pectin Substances 0.000 claims 1
- 229920001277 pectin Polymers 0.000 claims 1
- 235000010987 pectin Nutrition 0.000 claims 1
- 239000002002 slurry Substances 0.000 claims 1
- 230000008719 thickening Effects 0.000 claims 1
Claims (12)
- 下記の（ａ）で示される遺伝子群から選択されるポリガラクツロナーゼ遺伝子、及び（ｂ）で示される遺伝子群から選択されるペクチンリアーゼ遺伝子の両方を導入した組み換え糸状菌を培養することを含む、バイオマス糖化酵素の製造方法。
（ａ）ＰｇａＢ遺伝子、ＰｇａＩ遺伝子、ＰｇａＩＩ遺伝子
（ｂ）ＰｅｌＤ遺伝子、ＰｅｌＦ遺伝子 - ポリガラクツロナーゼ遺伝子がアスペルギルス・ニガーのＰｇａＢ遺伝子又は当該ＰｇａＢ遺伝子に相当する遺伝子であり、ペクチンリアーゼ遺伝子がアスペルギルス・ニガーのＰｅｌＤ又は当該ＰｅｌＤ遺伝子に相当する遺伝子である、請求項１に記載の方法。
- バイオマスがセルロース及びペクチンを含むバイオマスである、請求項１に記載の方法。
- バイオマスがキャッサバである、請求項１に記載の方法。
- 糸状菌がセルラーゼを生産する糸状菌である、請求項１に記載の方法。
- 糸状菌がトリコデルマ属の糸状菌である、請求項１～５のいずれか１項に記載の方法。
- 糸状菌がトリコデルマ・リーセイである、請求項６に記載の方法。
- 下記の（ａ）で示される遺伝子群から選択されるポリガラクツロナーゼ遺伝子、及び（ｂ）で示される遺伝子群から選択されるペクチンリアーゼ遺伝子の両方を導入した組み換え糸状菌を培養して得られる培養物をバイオマス糖化剤として用いる、バイオマスの糖化方法。
（ａ）ＰｇａＢ遺伝子、ＰｇａＩ遺伝子、ＰｇａＩＩ遺伝子
（ｂ）ＰｅｌＤ遺伝子、ＰｅｌＦ遺伝子 - スラリーの高粘度化を抑制する、請求項８に記載の方法。
- 糸状菌がセルラーゼを生産する糸状菌である、請求項８に記載の方法。
- 糸状菌がトリコデルマ属の糸状菌である、請求項８～１０のいずれか１項に記載の方法。
- 糸状菌がトリコデルマ・リーセイである、請求項１１に記載の方法。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021154749 | 2021-09-22 | ||
| JP2021154749 | 2021-09-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023046320A JP2023046320A (ja) | 2023-04-03 |
| JP2023046320A5 true JP2023046320A5 (ja) | 2024-05-29 |
Family
ID=85720765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022150677A Pending JP2023046320A (ja) | 2021-09-22 | 2022-09-21 | 糖化酵素の製造方法 |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP2023046320A (ja) |
| CN (1) | CN117999349A (ja) |
| WO (1) | WO2023048199A1 (ja) |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5937953B2 (ja) * | 1981-02-27 | 1984-09-12 | 工業技術院長 | 澱粉質原料の処理方法 |
| US5298405A (en) * | 1986-04-30 | 1994-03-29 | Alko Limited | Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production |
| EP0421919A3 (en) * | 1989-09-02 | 1992-04-15 | Ciba-Geigy Ag | Polygalacturonase encoding fungal expression system |
| DE102008024778A1 (de) * | 2008-05-23 | 2009-11-26 | Ab Enzymes Gmbh | Verwendung von pektinolytischen Enzymen zur Behandlung von Obst- und Gemüsemaische und Enzymsequenzen dazu |
| CN101285058B (zh) | 2008-06-11 | 2010-06-02 | 吴鹏 | 木薯渣工业用复合酶制剂 |
| JP5824074B2 (ja) * | 2012-01-30 | 2015-11-25 | サッポロビール株式会社 | バイオエタノールの製造方法及び製造システム |
| WO2018099877A1 (en) * | 2016-12-02 | 2018-06-07 | Dsm Ip Assets B.V. | Process for preparing soluble fibre |
| CN108004153B (zh) * | 2017-12-07 | 2021-05-28 | 潍坊康地恩生物科技有限公司 | 一株高产果胶裂解酶的里氏木霉菌株及其应用 |
-
2022
- 2022-09-21 CN CN202280064354.5A patent/CN117999349A/zh active Pending
- 2022-09-21 WO PCT/JP2022/035249 patent/WO2023048199A1/ja active Application Filing
- 2022-09-21 JP JP2022150677A patent/JP2023046320A/ja active Pending
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