JP2022534332A - 角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地 - Google Patents
角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地 Download PDFInfo
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Abstract
Description
1;遺伝子トランスフェクションを必要としないので、遺伝子改変と癌のリスクがない
2;誘導ステップが少ない
3;誘導時間が短い
4;誘導分化効率が高い
5;本発明の誘導剤、各成分はいずれも安全で毒性がない
6;間葉系幹細胞を膜上皮細胞に誘導分化した後、移植後に拒絶反応がなく、倫理問題がなく、安全性が高く、広い臨床応用の将来性がある。
本発明の角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地の各成分は以下の市販製品である:レスベラトロール、ブランドSigma、品番R5010;イカリイン、ブランド上海微晶生物,品番489-32-7;アスピリン、ブランドSigma、品番A2093-100G;副甲状腺ホルモン、ブランドSigma,品番P7036;ヒドロコルチゾン、ブランドSigma,品番H3160;ラパマイシン、ブランドTargetMol、品番T1537、テストステロン、ブランドSigma,品番T1500;EPO(エリスロポエチン)、ブランドPeproTech、品番CYT-201;LIF(白血病インヒビター因子)、ブランドPeproTech、品番96-300-05-5;角膜上皮細胞無血清培地(CEpiCM)、ブランドScienCell、品番6511。
角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地であって、以下の成分を、それぞれ以下の濃度で配合してなる:1Lごとの前記分化誘導無血清完全培地中にレスベラトロール5μmol、イカリイン2μmol、アスピリン1nmol、副甲状腺ホルモン1nmol、ヒドロコルチゾン5nmol、ラパマイシン1mg、テストステロン2μg、EPO 2μg、LIF 2μgが含まれ、残部は角膜上皮細胞の無血清培地であり、均一に混合した後にろ過して除菌すればよいものである。
角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地であって、以下の成分を、それぞれ以下の濃度で配合してなる:1Lごとの前記分化誘導無血清完全培地中にレスベラトロール10μmol、イカリイン4μmol、アスピリン3nmol、副甲状腺ホルモン3nmol、ヒドロコルチゾン10nmol、ラパマイシン3mg、テストステロン10μg、EPO 10μg、LIF 10μgが含まれ、残部は角膜上皮細胞の無血清培地であり、均一に混合した後にろ過して除菌すればよいものである。
ヒト脂肪間葉系幹細胞を例にして、実施例1で製造された誘導培地を用いて、間葉系幹細胞に対して角膜上皮細胞への誘導分化実験を行い、ステップは以下のとおりである。
表1 実験グループ分け
表2 3グループ誘導結果(n=20、`x±s)
Claims (2)
- 角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地であって、以下の方法で製造される:1Lごとの前記分化誘導無血清完全培地中にレスベラトロール5-10μmol、イカリイン2-4μmol、アスピリン1-3nmol、副甲状腺ホルモン1-3nmol、ヒドロコルチゾン5-10nmol、ラパマイシン1-3mg、テストステロン2-10μg、EPO 2-10μg、LIF 2-10μgが含まれ、残部は角膜上皮細胞の無血清培地であり、均一に混合した後にろ過して除菌すればよいものである、
ことを特徴とする角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地。 - 角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地であって、以下の方法で製造される:1Lごとの前記分化誘導無血清完全培地中にレスベラトロール8μmol、イカリイン3μmol、アスピリン2nmol、副甲状腺ホルモン2nmol、ヒドロコルチゾン7nmol、ラパマイシン2mg、テストステロン7μg、EPO 7μg、LIF 7μgが含まれ、残部は角膜上皮細胞の無血清培地であり、均一に混合した後にろ過して除菌すればよいものである、
ことを特徴とする角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地。
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CN202010310198.9 | 2020-04-20 | ||
PCT/CN2020/092071 WO2021212592A1 (zh) | 2020-04-20 | 2020-05-25 | 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 |
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