CN104419661B - 神经干细胞的制备方法 - Google Patents
神经干细胞的制备方法 Download PDFInfo
- Publication number
- CN104419661B CN104419661B CN201310380429.3A CN201310380429A CN104419661B CN 104419661 B CN104419661 B CN 104419661B CN 201310380429 A CN201310380429 A CN 201310380429A CN 104419661 B CN104419661 B CN 104419661B
- Authority
- CN
- China
- Prior art keywords
- culture medium
- culture
- nsc
- cell
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims abstract description 103
- 210000004027 cell Anatomy 0.000 claims abstract description 81
- 210000000130 stem cell Anatomy 0.000 claims abstract description 70
- 210000002569 neuron Anatomy 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 13
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 34
- 239000000654 additive Substances 0.000 claims description 32
- 239000007640 basal medium Substances 0.000 claims description 32
- 230000004069 differentiation Effects 0.000 claims description 29
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical class OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 18
- 210000002966 serum Anatomy 0.000 claims description 14
- 239000000835 fiber Substances 0.000 claims description 12
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims description 8
- 229930002330 retinoic acid Natural products 0.000 claims description 8
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 239000003102 growth factor Substances 0.000 claims description 7
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 6
- 102000004219 Brain-derived neurotrophic factor Human genes 0.000 claims description 5
- 108090000715 Brain-derived neurotrophic factor Proteins 0.000 claims description 5
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims description 5
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims description 5
- 102000016549 Vascular Endothelial Growth Factor Receptor-2 Human genes 0.000 claims description 5
- 230000010261 cell growth Effects 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 108010053099 Vascular Endothelial Growth Factor Receptor-2 Proteins 0.000 claims description 3
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 2
- 238000007792 addition Methods 0.000 claims 4
- 230000014509 gene expression Effects 0.000 abstract description 18
- 230000001939 inductive effect Effects 0.000 abstract description 5
- 210000002901 mesenchymal stem cell Anatomy 0.000 abstract description 4
- 210000001178 neural stem cell Anatomy 0.000 description 62
- 230000006698 induction Effects 0.000 description 18
- 239000007788 liquid Substances 0.000 description 18
- 230000003321 amplification Effects 0.000 description 11
- 238000003199 nucleic acid amplification method Methods 0.000 description 11
- 101100310657 Mus musculus Sox1 gene Proteins 0.000 description 10
- 102000008730 Nestin Human genes 0.000 description 10
- 108010088225 Nestin Proteins 0.000 description 10
- 101150081664 PAX6 gene Proteins 0.000 description 10
- 102000013127 Vimentin Human genes 0.000 description 10
- 108010065472 Vimentin Proteins 0.000 description 10
- 238000001514 detection method Methods 0.000 description 10
- 210000005055 nestin Anatomy 0.000 description 10
- 210000005048 vimentin Anatomy 0.000 description 10
- 102000007354 PAX6 Transcription Factor Human genes 0.000 description 9
- 210000005036 nerve Anatomy 0.000 description 9
- 230000001413 cellular effect Effects 0.000 description 7
- 208000015122 neurodegenerative disease Diseases 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000004043 dyeing Methods 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 5
- 239000002585 base Substances 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 229910052708 sodium Inorganic materials 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- 241000283707 Capra Species 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 4
- 238000010166 immunofluorescence Methods 0.000 description 4
- GRHBQAYDJPGGLF-UHFFFAOYSA-N isothiocyanic acid Chemical compound N=C=S GRHBQAYDJPGGLF-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000001537 neural effect Effects 0.000 description 4
- 230000004770 neurodegeneration Effects 0.000 description 4
- 210000004248 oligodendroglia Anatomy 0.000 description 4
- 238000012402 patch clamp technique Methods 0.000 description 4
- 230000003248 secreting effect Effects 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 102100032912 CD44 antigen Human genes 0.000 description 3
- 102100037241 Endoglin Human genes 0.000 description 3
- 102000006354 HLA-DR Antigens Human genes 0.000 description 3
- 108010058597 HLA-DR Antigens Proteins 0.000 description 3
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 3
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 3
- 101000881679 Homo sapiens Endoglin Proteins 0.000 description 3
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 3
- 101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 description 3
- 101000969087 Homo sapiens Microtubule-associated protein 2 Proteins 0.000 description 3
- 101000622304 Homo sapiens Vascular cell adhesion protein 1 Proteins 0.000 description 3
- 102100025304 Integrin beta-1 Human genes 0.000 description 3
- 108010025020 Nerve Growth Factor Proteins 0.000 description 3
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 description 3
- 102100023543 Vascular cell adhesion protein 1 Human genes 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 230000006907 apoptotic process Effects 0.000 description 3
- 210000001130 astrocyte Anatomy 0.000 description 3
- 230000006931 brain damage Effects 0.000 description 3
- 231100000874 brain damage Toxicity 0.000 description 3
- 208000029028 brain injury Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000004064 dysfunction Effects 0.000 description 3
- 210000001671 embryonic stem cell Anatomy 0.000 description 3
- 210000002304 esc Anatomy 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 229960001727 tretinoin Drugs 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 108010081589 Becaplermin Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000034615 Glial cell line-derived neurotrophic factor Human genes 0.000 description 2
- 108091010837 Glial cell line-derived neurotrophic factor Proteins 0.000 description 2
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 description 2
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 description 2
- 101000979001 Homo sapiens Methionine aminopeptidase 2 Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 101150088608 Kdr gene Proteins 0.000 description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
- 102100023174 Methionine aminopeptidase 2 Human genes 0.000 description 2
- 102000015336 Nerve Growth Factor Human genes 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- 239000002981 blocking agent Substances 0.000 description 2
- 210000001772 blood platelet Anatomy 0.000 description 2
- 210000005013 brain tissue Anatomy 0.000 description 2
- 210000005056 cell body Anatomy 0.000 description 2
- 238000003501 co-culture Methods 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000003722 extracellular fluid Anatomy 0.000 description 2
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000005847 immunogenicity Effects 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 210000003716 mesoderm Anatomy 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 210000004498 neuroglial cell Anatomy 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 208000037922 refractory disease Diseases 0.000 description 2
- 230000001172 regenerating effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 238000011476 stem cell transplantation Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000001228 trophic effect Effects 0.000 description 2
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 239000012583 B-27 Supplement Substances 0.000 description 1
- -1 CD31 Proteins 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 101000888419 Homo sapiens Glial fibrillary acidic protein Proteins 0.000 description 1
- 101001111320 Homo sapiens Nestin Proteins 0.000 description 1
- 101000803403 Homo sapiens Vimentin Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 239000012580 N-2 Supplement Substances 0.000 description 1
- 102000007072 Nerve Growth Factors Human genes 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108050008758 Transcription factor S Proteins 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 238000011316 allogeneic transplantation Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 239000002771 cell marker Substances 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000004720 cerebrum Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- 239000006160 differential media Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003237 epithelioid cell Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229940126864 fibroblast growth factor Drugs 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 210000001320 hippocampus Anatomy 0.000 description 1
- 102000051520 human GFAP Human genes 0.000 description 1
- 102000046587 human MAP2 Human genes 0.000 description 1
- 102000043377 human NES Human genes 0.000 description 1
- 102000057393 human VIM Human genes 0.000 description 1
- 238000013394 immunophenotyping Methods 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000009940 knitting Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000007659 motor function Effects 0.000 description 1
- 230000007830 nerve conduction Effects 0.000 description 1
- 229940053128 nerve growth factor Drugs 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000007472 neurodevelopment Effects 0.000 description 1
- 230000004031 neuronal differentiation Effects 0.000 description 1
- 230000000508 neurotrophic effect Effects 0.000 description 1
- 239000003900 neurotrophic factor Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000009256 replacement therapy Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000004508 retinoic acid derivatives Chemical class 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (13)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310380429.3A CN104419661B (zh) | 2013-08-28 | 2013-08-28 | 神经干细胞的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310380429.3A CN104419661B (zh) | 2013-08-28 | 2013-08-28 | 神经干细胞的制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104419661A CN104419661A (zh) | 2015-03-18 |
CN104419661B true CN104419661B (zh) | 2017-11-21 |
Family
ID=52969785
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310380429.3A Active CN104419661B (zh) | 2013-08-28 | 2013-08-28 | 神经干细胞的制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104419661B (zh) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105062970B (zh) * | 2015-08-25 | 2017-12-29 | 青岛瑞思科生物科技有限公司 | 一种将间充质干细胞诱导成神经细胞的诱导剂及诱导分化完全培养基 |
CN105420193B (zh) * | 2015-12-07 | 2019-04-09 | 中国科学院广州生物医药与健康研究院 | 分化培养基及其在制备神经干细胞中的用途 |
CN105567638A (zh) * | 2016-03-01 | 2016-05-11 | 赛百慷(上海)生物技术股份有限公司 | 一种神经干细胞添加剂 |
CN106619722A (zh) * | 2016-12-05 | 2017-05-10 | 上海安集协康生物技术股份有限公司 | 一种治疗脑部损伤类疾病的神经干细胞注射液 |
CN109266610B (zh) * | 2018-09-19 | 2021-06-25 | 宁波金未生物科技有限公司 | 一种促进间充质干细胞分化为神经元的方法 |
CN109852581B (zh) * | 2019-01-07 | 2020-08-21 | 北京健坤禾润科技有限公司 | 一种促进干细胞成脂分化的方法及其所用试剂或试剂盒 |
CN111235110A (zh) * | 2020-01-14 | 2020-06-05 | 河南省银丰生物工程技术有限公司 | 一种神经干细胞的体外培养方法 |
CN111334474A (zh) * | 2020-02-25 | 2020-06-26 | 河南省银丰生物工程技术有限公司 | 一种用于神经干细胞的体外移植培养方法 |
CN112795541B (zh) * | 2020-11-18 | 2023-02-10 | 天津市康婷生物工程集团有限公司 | 一种脐带间充质干细胞诱导神经干细胞分化成神经元和星胶质细胞的方法 |
CN114438031A (zh) * | 2022-02-21 | 2022-05-06 | 赛尔生命科技(深圳)有限公司 | 诱导型神经干细胞培养液及制备方法 |
CN114525250B (zh) * | 2022-04-25 | 2022-08-05 | 深圳博雅感知药业有限公司 | 一种肿瘤干细胞向神经元方向分化方法 |
CN116555260B (zh) * | 2023-04-24 | 2024-05-28 | 中山大学中山眼科中心 | 一种对人iPSCs进行基因编辑制备神经干细胞的方法 |
CN118028229B (zh) * | 2024-04-11 | 2024-06-21 | 成都云测医学生物技术有限公司 | 一种从神经类器官获得间充质干细胞的制备方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101831401A (zh) * | 2010-04-23 | 2010-09-15 | 天津昂赛细胞基因工程有限公司 | 体外诱导间充质干细胞分化为神经干细胞的方法 |
CN103013917A (zh) * | 2012-11-30 | 2013-04-03 | 陆华 | 人羊膜间充质干细胞分化为神经元样细胞的诱导方法 |
CN103031275A (zh) * | 2012-11-30 | 2013-04-10 | 陆华 | 脐带间充质干细胞分化为神经干细胞的诱导方法 |
-
2013
- 2013-08-28 CN CN201310380429.3A patent/CN104419661B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN104419661A (zh) | 2015-03-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104419661B (zh) | 神经干细胞的制备方法 | |
Chang et al. | Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media | |
Peng et al. | Human umbilical cord Wharton's jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro | |
Achilleos et al. | Neural crest stem cells: discovery, properties and potential for therapy | |
CN110396499A (zh) | 一种诱导神经干细胞的方法及其应用 | |
Arnhold et al. | Human bone marrow stroma cells display certain neural characteristics and integrate in the subventricular compartment after injection into the liquor system | |
JP6824267B2 (ja) | ヒト誘導多能性幹細胞からライディッヒ細胞への分化誘導方法及びその用途 | |
CN101314766B (zh) | 一种分离培养人脂肪间充质干细胞的方法及其专用培养基 | |
CN104946590B (zh) | 成人骨髓中Muse细胞诱导为神经前体细胞的方法 | |
Kim et al. | Engineering three dimensional micro nerve tissue using postnatal stem cells from human dental apical papilla | |
JP5388297B2 (ja) | アディポクラスター | |
Li et al. | Engraftable neural crest stem cells derived from cynomolgus monkey embryonic stem cells | |
CN112891374A (zh) | 治疗中风的影响的方法 | |
CN103865873B (zh) | 亚全能干细胞分泌的外来体及其应用 | |
Jamali et al. | Differentiation of mesenchymal stem cells-derived trabecular meshwork into dopaminergic neuron-like cells on nanofibrous scaffolds | |
AU2013225946B2 (en) | Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation | |
CN105087475B (zh) | 一种细胞培养液及其应用以及诱导牙髓干细胞向神经样细胞分化的方法 | |
WO2023165062A1 (zh) | 一种少突胶质细胞的制备方法及应用 | |
US20180171291A1 (en) | Derivation of endothelial cells from mammalian pluirpotent stem cells | |
CN109385399A (zh) | 一种羊水间充质干细胞分化为神经干细胞的方法 | |
JP6980211B2 (ja) | 末梢神経細胞の製造方法 | |
TWI490337B (zh) | 將脂肪幹細胞培養成球狀體並使其神經分化之培養材料及方法 | |
El-Hashash | Neural crest stem cells: a therapeutic hope machine for neural regeneration | |
AU2018102120A4 (en) | Preparation and expansion methods for human pluripotent stem cell-derived human retinal pigment epithelial cells | |
TWI573873B (zh) | 體外無血清成體幹細胞放大培養技術 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20190202 Address after: 262700 Middle Section of Xiangyang Road, Yangkou Town, Shouguang City, Shandong Province Patentee after: Shouguang Jinwo Real Estate Co., Ltd. Address before: 100005 Dongdan No. 3, No. 5, Dongcheng District, Beijing Patentee before: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20200325 Address after: 266200 Room 601, building B, No. 168, Ningdong Road, Blue New Area, Jimo Provincial Economic Development Zone, Qingdao, Shandong Province Patentee after: QINGDAO GUOAOYUAN BIOENGINEERING TECHNOLOGY GROUP Co.,Ltd. Address before: 262700 Middle Section of Xiangyang Road, Yangkou Town, Shouguang City, Shandong Province Patentee before: Shouguang Jinwo Real Estate Co., Ltd. |
|
TR01 | Transfer of patent right | ||
CP01 | Change in the name or title of a patent holder |
Address after: 266200 Room 601, building B, No. 168, Ningdong Road, Blue New Area, Jimo Provincial Economic Development Zone, Qingdao, Shandong Province Patentee after: Qingdao Hualing Aoyuan Bioengineering Technology Group Co.,Ltd. Address before: 266200 Room 601, building B, No. 168, Ningdong Road, Blue New Area, Jimo Provincial Economic Development Zone, Qingdao, Shandong Province Patentee before: QINGDAO GUOAOYUAN BIOENGINEERING TECHNOLOGY GROUP Co.,Ltd. |
|
CP01 | Change in the name or title of a patent holder | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210728 Address after: 266000 No.168 Ningdong Road, Blue New District, Jimo Economic Development Zone, Qingdao City, Shandong Province Patentee after: Qingdao Guoaoyuan Huasheng Biotechnology Group Co.,Ltd. Address before: Room 601, building B, 168 Ningdong Road, Blue New District, Jimo Provincial Economic Development Zone, Qingdao, Shandong 266200 Patentee before: Qingdao Hualing Aoyuan Bioengineering Technology Group Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
CP01 | Change in the name or title of a patent holder |
Address after: 266000 No.168 Ningdong Road, Blue New District, Jimo Economic Development Zone, Qingdao City, Shandong Province Patentee after: Micro energy Life Technology Group Co.,Ltd. Address before: 266000 No.168 Ningdong Road, Blue New District, Jimo Economic Development Zone, Qingdao City, Shandong Province Patentee before: Qingdao Guoaoyuan Huasheng Biotechnology Group Co.,Ltd. |
|
CP01 | Change in the name or title of a patent holder |