JP2022520663A5 - - Google Patents

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Publication number
JP2022520663A5
JP2022520663A5 JP2021548194A JP2021548194A JP2022520663A5 JP 2022520663 A5 JP2022520663 A5 JP 2022520663A5 JP 2021548194 A JP2021548194 A JP 2021548194A JP 2021548194 A JP2021548194 A JP 2021548194A JP 2022520663 A5 JP2022520663 A5 JP 2022520663A5
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JP
Japan
Prior art keywords
subsets
probe
amplifier
labeling
target
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JP2021548194A
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English (en)
Japanese (ja)
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JP7531502B2 (ja
JP2022520663A (ja
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Priority claimed from PCT/US2020/018241 external-priority patent/WO2020168162A1/en
Publication of JP2022520663A publication Critical patent/JP2022520663A/ja
Publication of JP2022520663A5 publication Critical patent/JP2022520663A5/ja
Application granted granted Critical
Publication of JP7531502B2 publication Critical patent/JP7531502B2/ja
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JP2021548194A 2019-02-15 2020-02-14 ｉｎ ｓｉｔｕハイブリダイゼーションによって、核酸をマルチプレックス検出する方法 Active JP7531502B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962806574P 2019-02-15 2019-02-15
US62/806,574 2019-02-15
PCT/US2020/018241 WO2020168162A1 (en) 2019-02-15 2020-02-14 Methods for multiplex detection of nucleic acids by in situ hybridization

Publications (3)

Publication Number Publication Date
JP2022520663A JP2022520663A (ja) 2022-03-31
JP2022520663A5 true JP2022520663A5 (https=) 2023-02-17
JP7531502B2 JP7531502B2 (ja) 2024-08-09

Family

ID=72044135

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021548194A Active JP7531502B2 (ja) 2019-02-15 2020-02-14 ｉｎ ｓｉｔｕハイブリダイゼーションによって、核酸をマルチプレックス検出する方法

Country Status (6)

Country Link
US (2) US12571028B2 (https=)
EP (1) EP3924501A4 (https=)
JP (1) JP7531502B2 (https=)
CN (1) CN114555825A (https=)
SG (1) SG11202108618VA (https=)
WO (1) WO2020168162A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3874057A4 (en) * 2018-11-01 2022-08-17 Advanced Cell Diagnostics, Inc. METHODS FOR DIGITAL MULTIPLEXING OF NUCLEIC ACIDS IN SITU
US20230002815A1 (en) * 2019-11-20 2023-01-05 Advanced Cell Diagnostics, Inc. Methods for sequential detection of nucleic acids
WO2022221637A1 (en) * 2021-04-16 2022-10-20 Advanced Cell Diagnostics, Inc. Methods and compositions for reducing autofluorescence
US20240212150A1 (en) 2021-04-16 2024-06-27 Advanced Cel Diagnostics, Inc. Materials and methods related to image processing
WO2024124041A1 (en) * 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets

Family Cites Families (26)

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Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5849481A (en) 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US6465175B2 (en) 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
DK2500439T4 (da) 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
US20090081688A1 (en) 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
WO2007090071A2 (en) 2006-01-27 2007-08-09 Isis Pharmaceuticals, Inc. 6-modified bicyclic nucleic acid analogs
WO2011038403A1 (en) * 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US8658361B2 (en) 2010-10-21 2014-02-25 Advanced Cell Diagnostics, Inc. Ultra sensitive method for in situ detection of nucleic acids
WO2016025867A1 (en) 2014-08-15 2016-02-18 Affymetrix, Inc. Robust detection of nucleic acids in situ
EP3362462B1 (en) 2015-10-12 2021-07-21 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
AU2017302300B2 (en) 2016-07-27 2023-08-17 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
ES2981703T3 (es) * 2016-09-02 2024-10-10 Lentigen Tech Inc Composiciones y métodos para tratar cáncer con DuoCars
US11352660B2 (en) * 2016-10-04 2022-06-07 Howard Hughes Medical Institute Materials and methods for serial multiplexed detection of RNA in cells and tissues
EP4339956A3 (en) 2016-11-08 2024-06-19 President and Fellows of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
DK3555313T3 (da) * 2016-12-16 2021-08-09 Aratome Llc Molekylær detektering ved hjælp af ligeringsamplifikation
EP3874057A4 (en) * 2018-11-01 2022-08-17 Advanced Cell Diagnostics, Inc. METHODS FOR DIGITAL MULTIPLEXING OF NUCLEIC ACIDS IN SITU
US20230002815A1 (en) * 2019-11-20 2023-01-05 Advanced Cell Diagnostics, Inc. Methods for sequential detection of nucleic acids
WO2024124041A1 (en) * 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets

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