JP7531502B2 - ｉｎ ｓｉｔｕハイブリダイゼーションによって、核酸をマルチプレックス検出する方法 - Google Patents

ｉｎ ｓｉｔｕハイブリダイゼーションによって、核酸をマルチプレックス検出する方法 Download PDF

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JP7531502B2
JP7531502B2 JP2021548194A JP2021548194A JP7531502B2 JP 7531502 B2 JP7531502 B2 JP 7531502B2 JP 2021548194 A JP2021548194 A JP 2021548194A JP 2021548194 A JP2021548194 A JP 2021548194A JP 7531502 B2 JP7531502 B2 JP 7531502B2
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labeled probe
subsets
probe
target
target nucleic
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JP2022520663A5 (https=
JP2022520663A (ja
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マ，シャオ－チュン
チャン，ピンチン
ワン，リ－チョン
ルー，ハン
ワン，リ
ヅォン，ヘイリン
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アドヴァンスド セル ダイアグノスティクス，インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021548194A 2019-02-15 2020-02-14 ｉｎ ｓｉｔｕハイブリダイゼーションによって、核酸をマルチプレックス検出する方法 Active JP7531502B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962806574P 2019-02-15 2019-02-15
US62/806,574 2019-02-15
PCT/US2020/018241 WO2020168162A1 (en) 2019-02-15 2020-02-14 Methods for multiplex detection of nucleic acids by in situ hybridization

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JP2022520663A JP2022520663A (ja) 2022-03-31
JP2022520663A5 JP2022520663A5 (https=) 2023-02-17
JP7531502B2 true JP7531502B2 (ja) 2024-08-09

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US (2) US12571028B2 (https=)
EP (1) EP3924501A4 (https=)
JP (1) JP7531502B2 (https=)
CN (1) CN114555825A (https=)
SG (1) SG11202108618VA (https=)
WO (1) WO2020168162A1 (https=)

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Publication number Priority date Publication date Assignee Title
EP3874057A4 (en) * 2018-11-01 2022-08-17 Advanced Cell Diagnostics, Inc. METHODS FOR DIGITAL MULTIPLEXING OF NUCLEIC ACIDS IN SITU
US20230002815A1 (en) * 2019-11-20 2023-01-05 Advanced Cell Diagnostics, Inc. Methods for sequential detection of nucleic acids
WO2022221637A1 (en) * 2021-04-16 2022-10-20 Advanced Cell Diagnostics, Inc. Methods and compositions for reducing autofluorescence
US20240212150A1 (en) 2021-04-16 2024-06-27 Advanced Cel Diagnostics, Inc. Materials and methods related to image processing
WO2024124041A1 (en) * 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets

Citations (2)

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WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US20180094305A1 (en) 2016-10-04 2018-04-05 Howard Hughes Medical Institute Materials and methods for serial multiplexed detection of rna in cells and tissues

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US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
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US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
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US6465175B2 (en) 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
DK2500439T4 (da) 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
US20090081688A1 (en) 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
WO2007090071A2 (en) 2006-01-27 2007-08-09 Isis Pharmaceuticals, Inc. 6-modified bicyclic nucleic acid analogs
US8658361B2 (en) 2010-10-21 2014-02-25 Advanced Cell Diagnostics, Inc. Ultra sensitive method for in situ detection of nucleic acids
WO2016025867A1 (en) 2014-08-15 2016-02-18 Affymetrix, Inc. Robust detection of nucleic acids in situ
EP3362462B1 (en) 2015-10-12 2021-07-21 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
AU2017302300B2 (en) 2016-07-27 2023-08-17 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
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US20230002815A1 (en) * 2019-11-20 2023-01-05 Advanced Cell Diagnostics, Inc. Methods for sequential detection of nucleic acids
WO2024124041A1 (en) * 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets

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WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US20180094305A1 (en) 2016-10-04 2018-04-05 Howard Hughes Medical Institute Materials and methods for serial multiplexed detection of rna in cells and tissues

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J. Mol. Diagn.，2012年，Vol.14, No.1，pp.22-29
Proc. Natl. Acad. Sci.，2016年，Vol.113, No.39，pp.11046-11051

Also Published As

Publication number Publication date
US12571028B2 (en) 2026-03-10
WO2020168162A1 (en) 2020-08-20
EP3924501A1 (en) 2021-12-22
US20260110019A1 (en) 2026-04-23
EP3924501A4 (en) 2022-11-23
CN114555825A (zh) 2022-05-27
US20220154262A1 (en) 2022-05-19
SG11202108618VA (en) 2021-09-29
JP2022520663A (ja) 2022-03-31

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