SG11202108618VA - Methods for multiplex detection of nucleic acids by in situ hybridization - Google Patents
Methods for multiplex detection of nucleic acids by in situ hybridizationInfo
- Publication number
- SG11202108618VA SG11202108618VA SG11202108618VA SG11202108618VA SG11202108618VA SG 11202108618V A SG11202108618V A SG 11202108618VA SG 11202108618V A SG11202108618V A SG 11202108618VA SG 11202108618V A SG11202108618V A SG 11202108618VA SG 11202108618V A SG11202108618V A SG 11202108618VA
- Authority
- SG
- Singapore
- Prior art keywords
- methods
- nucleic acids
- situ hybridization
- multiplex detection
- multiplex
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962806574P | 2019-02-15 | 2019-02-15 | |
| PCT/US2020/018241 WO2020168162A1 (en) | 2019-02-15 | 2020-02-14 | Methods for multiplex detection of nucleic acids by in situ hybridization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202108618VA true SG11202108618VA (en) | 2021-09-29 |
Family
ID=72044135
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202108618VA SG11202108618VA (en) | 2019-02-15 | 2020-02-14 | Methods for multiplex detection of nucleic acids by in situ hybridization |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20220154262A1 (en) |
| EP (1) | EP3924501A4 (en) |
| JP (1) | JP2022520663A (en) |
| CN (1) | CN114555825A (en) |
| SG (1) | SG11202108618VA (en) |
| WO (1) | WO2020168162A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113166800A (en) * | 2018-11-01 | 2021-07-23 | 领先细胞医疗诊断有限公司 | Method for in situ nucleic acid digital multiplexing |
| AU2020386050A1 (en) * | 2019-11-20 | 2022-06-23 | Advanced Cell Diagnostics, Inc. | Methods for sequential detection of nucleic acids |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154598B (en) | 1970-11-10 | 1977-09-15 | Organon Nv | PROCEDURE FOR DETERMINING AND DETERMINING LOW MOLECULAR COMPOUNDS AND PROTEINS THAT CAN SPECIFICALLY BIND THESE COMPOUNDS AND TEST PACKAGING. |
| US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5849481A (en) | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US20090081688A1 (en) | 2005-06-20 | 2009-03-26 | Advanced Cell Diagnostics | Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations |
| ES2565670T3 (en) | 2005-06-20 | 2016-04-06 | Advanced Cell Diagnostics, Inc. | Nucleic acid detection methods in individual cells and identification of rare cells in large populations of heterogeneous cells |
| KR20130042043A (en) | 2006-01-27 | 2013-04-25 | 아이시스 파마수티컬즈 인코포레이티드 | 6-modified bicyclic nucleic acid analogs |
| US20130023433A1 (en) | 2009-09-28 | 2013-01-24 | Yuling Luo | Methods of detecting nucleic acid sequences with high specificity |
| CN104849472A (en) | 2010-10-21 | 2015-08-19 | 领先细胞医疗诊断有限公司 | Ultra sensitive method for in situ detection of nucleic acids |
| ES2893100T3 (en) | 2015-10-12 | 2022-02-08 | Advanced Cell Diagnostics Inc | In situ detection of nucleotide variants in samples with a high level of noise, and related compositions and methods |
| US11352660B2 (en) * | 2016-10-04 | 2022-06-07 | Howard Hughes Medical Institute | Materials and methods for serial multiplexed detection of RNA in cells and tissues |
| US20190276881A1 (en) * | 2016-11-08 | 2019-09-12 | President And Fellows Of Harvard College | Multiplexed imaging using merfish, expansion microscopy, and related technologies |
-
2020
- 2020-02-14 WO PCT/US2020/018241 patent/WO2020168162A1/en unknown
- 2020-02-14 EP EP20755307.4A patent/EP3924501A4/en active Pending
- 2020-02-14 CN CN202080027949.4A patent/CN114555825A/en active Pending
- 2020-02-14 JP JP2021548194A patent/JP2022520663A/en active Pending
- 2020-02-14 US US17/430,463 patent/US20220154262A1/en active Pending
- 2020-02-14 SG SG11202108618VA patent/SG11202108618VA/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3924501A1 (en) | 2021-12-22 |
| CN114555825A (en) | 2022-05-27 |
| US20220154262A1 (en) | 2022-05-19 |
| EP3924501A4 (en) | 2022-11-23 |
| WO2020168162A1 (en) | 2020-08-20 |
| JP2022520663A (en) | 2022-03-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SG11202105109PA (en) | Method for detection of rna | |
| EP3640340A4 (en) | Method for detecting target nucleic acid sequences | |
| EP3530737A4 (en) | Method for identifying dna base editing by means of cytosine deaminase | |
| GB201904349D0 (en) | Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing | |
| SI3559267T1 (en) | Method for molecular barcoding of nucleic acids of single cells | |
| ZA202007778B (en) | Nucleic acid detection method | |
| GB2598497B (en) | Nucleic acid hybridization methods | |
| SG11201700765WA (en) | Detection of target nucleic acids using hybridization | |
| GB201701262D0 (en) | Nucleic acid detection method | |
| IL287706A (en) | Methods for detecting nucleic acid variants | |
| EP3269813A4 (en) | Method for separating nucleic acids from fepe tissue | |
| IL280772A (en) | Multiplex detection of nucleic acids | |
| EP3652345A4 (en) | Sequencing method for genomic rearrangement detection | |
| EP3667672A4 (en) | Method for detecting gene rearrangement by using next generation sequencing | |
| SG11202108618VA (en) | Methods for multiplex detection of nucleic acids by in situ hybridization | |
| GB201717445D0 (en) | Method for determining nucleotide sequence | |
| IL283989A (en) | Method for synthesizing single-stranded dna | |
| EP3279339A4 (en) | Method for determining gene state of fetus | |
| EP3467835C0 (en) | Improved alignment method for nucleic acid sequences | |
| EP3650553C0 (en) | Method for detection of specific nucleic acids | |
| IL269600A (en) | Method for high-throughput genomic dna extraction | |
| IL284065A (en) | Method for detecting polymerase incorporation of nucleotides | |
| IL277764B (en) | Method for detecting variation of reference sequence in target nucleic acid region | |
| EP3241900A4 (en) | Oligonucleotide probe for detecting single nucleotide polymorphism, and method for detecting single nucleotide polymorphism | |
| EP3453769B8 (en) | Multiplex nucleic acid detection method |