JP2021534408A - Ｄｅ ｎｏｖｏタンパク質シークエンシングのための方法 - Google Patents

Ｄｅ ｎｏｖｏタンパク質シークエンシングのための方法 Download PDF

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JP2021534408A
JP2021534408A JP2021507942A JP2021507942A JP2021534408A JP 2021534408 A JP2021534408 A JP 2021534408A JP 2021507942 A JP2021507942 A JP 2021507942A JP 2021507942 A JP2021507942 A JP 2021507942A JP 2021534408 A JP2021534408 A JP 2021534408A
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peptide
mass
amino acid
ions
fragmented
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JP2021534408A5 (https=
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ユアン マオ
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • H01J49/28—Static spectrometers
    • H01J49/30—Static spectrometers using magnetic analysers, e.g. Dempster spectrometer
    • H01J49/305—Static spectrometers using magnetic analysers, e.g. Dempster spectrometer with several sectors in tandem

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Evolutionary Biology (AREA)
  • Organic Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Computation (AREA)
  • Optics & Photonics (AREA)
  • Bioethics (AREA)
  • Artificial Intelligence (AREA)
  • Public Health (AREA)
  • Software Systems (AREA)
  • Databases & Information Systems (AREA)
  • Data Mining & Analysis (AREA)
  • Signal Processing (AREA)
  • Epidemiology (AREA)
JP2021507942A 2018-08-17 2019-08-16 Ｄｅ ｎｏｖｏタンパク質シークエンシングのための方法 Pending JP2021534408A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862719292P 2018-08-17 2018-08-17
US62/719,292 2018-08-17
PCT/US2019/046821 WO2020037205A1 (en) 2018-08-17 2019-08-16 Methods for de novo protein sequencing

Publications (2)

Publication Number Publication Date
JP2021534408A true JP2021534408A (ja) 2021-12-09
JP2021534408A5 JP2021534408A5 (https=) 2022-08-03

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JP2021507942A Pending JP2021534408A (ja) 2018-08-17 2019-08-16 Ｄｅ ｎｏｖｏタンパク質シークエンシングのための方法

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US (2) US11047863B2 (https=)
EP (1) EP3837553A1 (https=)
JP (1) JP2021534408A (https=)
KR (1) KR20210045449A (https=)
CN (1) CN112601962A (https=)
AU (1) AU2019320814A1 (https=)
BR (1) BR112021002029A2 (https=)
CA (1) CA3107963A1 (https=)
EA (1) EA202190550A1 (https=)
IL (1) IL280628A (https=)
MX (1) MX2021001923A (https=)
SG (1) SG11202100740YA (https=)
WO (1) WO2020037205A1 (https=)
ZA (1) ZA202100694B (https=)

Families Citing this family (5)

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Publication number Priority date Publication date Assignee Title
KR20210045449A (ko) * 2018-08-17 2021-04-26 리제너론 파아마슈티컬스, 인크. 새로운 단백질 서열분석 방법
AU2019380606B2 (en) 2018-11-15 2026-04-23 Quantum-Si Incorporated Methods and compositions for protein sequencing
US20210148922A1 (en) * 2019-10-28 2021-05-20 Quantum-Si Incorporated Methods of single-polypeptide sequencing and reconstruction
EP4143579A2 (en) 2020-05-20 2023-03-08 Quantum-si Incorporated Methods and compositions for protein sequencing
KR102691545B1 (ko) * 2023-12-28 2024-08-05 충남대학교 산학협력단 안정동위원소가 표지된 내부 표준 펩타이드를 이용한 질량분석 기반의 표적 펩타이드의 정량 방법

Citations (4)

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JP2007322293A (ja) * 2006-06-02 2007-12-13 Hitachi High-Technologies Corp デノボシーケンス解析方法，解析ソフト，解析ソフトを記憶した記憶媒体，試薬キット
JP2011112562A (ja) * 2009-11-27 2011-06-09 Mitsubishi Chemicals Corp ペプチド末端の同定方法
WO2017022562A1 (ja) * 2015-08-03 2017-02-09 株式会社島津製作所 タンパク質変異体の並行的定量方法
US9719078B1 (en) * 2014-06-15 2017-08-01 Darryl J. Pappin Proteases for the production of N-terminal argininyl- and lysinyl-peptides and methods of use in protein analysis

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US5824556A (en) * 1997-06-11 1998-10-20 Tarr; George E. Peptide mass ladders generated using carbon disulfide
US6962818B2 (en) * 2000-10-19 2005-11-08 Target Discovery Mass defect labeling for the determination of oligomer sequences
US20040185448A1 (en) * 2003-03-20 2004-09-23 Viorica Lopez-Avila Methods and devices for performing matrix assisted laser desorption/lonization protocols
GB2448562B (en) * 2006-10-24 2012-02-22 Bruker Daltonik Gmbh Top-down protein analysis in mass spectrometers with ion traps
GB0723183D0 (en) * 2007-11-23 2008-01-09 Micromass Ltd Mass spectrometer
ATE460666T1 (de) * 2008-01-15 2010-03-15 Univ Utrecht Holding Bv Verfahren zur bestimmung der aminosäurensequenz von peptiden
CN101298611B (zh) * 2008-06-12 2011-06-15 北京生命科学研究所 衣原体蛋白酶体样活性因子的活性片段及其表达方法
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
US8455624B2 (en) * 2011-06-03 2013-06-04 University Of South Alabama Methods and compositions for detecting endometrial or ovarian cancer
JP6281349B2 (ja) 2014-03-19 2018-02-21 株式会社島津製作所 質量分析用ペプチド混合物試料の調製方法
WO2015185592A2 (en) * 2014-06-03 2015-12-10 Dsm Ip Assets B.V. Proline-specific endoprotease and use thereof
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US11807671B2 (en) * 2016-11-16 2023-11-07 Auckland Uniservices Limited Methods for protein ligation and uses thereof
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KR20210045449A (ko) * 2018-08-17 2021-04-26 리제너론 파아마슈티컬스, 인크. 새로운 단백질 서열분석 방법

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JP2007322293A (ja) * 2006-06-02 2007-12-13 Hitachi High-Technologies Corp デノボシーケンス解析方法，解析ソフト，解析ソフトを記憶した記憶媒体，試薬キット
JP2011112562A (ja) * 2009-11-27 2011-06-09 Mitsubishi Chemicals Corp ペプチド末端の同定方法
US9719078B1 (en) * 2014-06-15 2017-08-01 Darryl J. Pappin Proteases for the production of N-terminal argininyl- and lysinyl-peptides and methods of use in protein analysis
WO2017022562A1 (ja) * 2015-08-03 2017-02-09 株式会社島津製作所 タンパク質変異体の並行的定量方法

Non-Patent Citations (1)

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Title
BROWNSTEIN, C. NAOMI ET AL: "Paired singleresidue-transposed Lys-N and Lys-C digestions for label-freeidentification of N-termina", RAPID COMMUN. MASS SPECTROM., vol. 29, JPN6023013337, 2015, pages 659 - 666, ISSN: 0005117714 *

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BR112021002029A2 (pt) 2021-05-25
EP3837553A1 (en) 2021-06-23
US11047863B2 (en) 2021-06-29
SG11202100740YA (en) 2021-02-25
MX2021001923A (es) 2021-04-28
US20210278414A1 (en) 2021-09-09
EA202190550A1 (ru) 2021-04-23
CA3107963A1 (en) 2020-02-20
CN112601962A (zh) 2021-04-02
US20200057073A1 (en) 2020-02-20
KR20210045449A (ko) 2021-04-26
IL280628A (en) 2021-03-25
AU2019320814A1 (en) 2021-02-18
US12000840B2 (en) 2024-06-04
WO2020037205A1 (en) 2020-02-20
ZA202100694B (en) 2023-03-29

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