CA3107963A1 - Methods for de novo protein sequencing - Google Patents

Methods for de novo protein sequencing Download PDF

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Publication number
CA3107963A1
CA3107963A1 CA3107963A CA3107963A CA3107963A1 CA 3107963 A1 CA3107963 A1 CA 3107963A1 CA 3107963 A CA3107963 A CA 3107963A CA 3107963 A CA3107963 A CA 3107963A CA 3107963 A1 CA3107963 A1 CA 3107963A1
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CA
Canada
Prior art keywords
peptide
ions
mass
amino acid
fragmented
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3107963A
Other languages
English (en)
French (fr)
Inventor
Yuan Mao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Regeneron Pharmaceuticals Inc
Original Assignee
Regeneron Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Publication of CA3107963A1 publication Critical patent/CA3107963A1/en
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • H01J49/28—Static spectrometers
    • H01J49/30—Static spectrometers using magnetic analysers, e.g. Dempster spectrometer
    • H01J49/305—Static spectrometers using magnetic analysers, e.g. Dempster spectrometer with several sectors in tandem

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Evolutionary Biology (AREA)
  • Organic Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Computation (AREA)
  • Optics & Photonics (AREA)
  • Bioethics (AREA)
  • Artificial Intelligence (AREA)
  • Public Health (AREA)
  • Software Systems (AREA)
  • Databases & Information Systems (AREA)
  • Data Mining & Analysis (AREA)
  • Signal Processing (AREA)
  • Epidemiology (AREA)
CA3107963A 2018-08-17 2019-08-16 Methods for de novo protein sequencing Pending CA3107963A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862719292P 2018-08-17 2018-08-17
US62/719,292 2018-08-17
PCT/US2019/046821 WO2020037205A1 (en) 2018-08-17 2019-08-16 Methods for de novo protein sequencing

Publications (1)

Publication Number Publication Date
CA3107963A1 true CA3107963A1 (en) 2020-02-20

Family

ID=67851219

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3107963A Pending CA3107963A1 (en) 2018-08-17 2019-08-16 Methods for de novo protein sequencing

Country Status (14)

Country Link
US (2) US11047863B2 (https=)
EP (1) EP3837553A1 (https=)
JP (1) JP2021534408A (https=)
KR (1) KR20210045449A (https=)
CN (1) CN112601962A (https=)
AU (1) AU2019320814A1 (https=)
BR (1) BR112021002029A2 (https=)
CA (1) CA3107963A1 (https=)
EA (1) EA202190550A1 (https=)
IL (1) IL280628A (https=)
MX (1) MX2021001923A (https=)
SG (1) SG11202100740YA (https=)
WO (1) WO2020037205A1 (https=)
ZA (1) ZA202100694B (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210045449A (ko) * 2018-08-17 2021-04-26 리제너론 파아마슈티컬스, 인크. 새로운 단백질 서열분석 방법
AU2019380606B2 (en) 2018-11-15 2026-04-23 Quantum-Si Incorporated Methods and compositions for protein sequencing
US20210148922A1 (en) * 2019-10-28 2021-05-20 Quantum-Si Incorporated Methods of single-polypeptide sequencing and reconstruction
EP4143579A2 (en) 2020-05-20 2023-03-08 Quantum-si Incorporated Methods and compositions for protein sequencing
KR102691545B1 (ko) * 2023-12-28 2024-08-05 충남대학교 산학협력단 안정동위원소가 표지된 내부 표준 펩타이드를 이용한 질량분석 기반의 표적 펩타이드의 정량 방법

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824556A (en) * 1997-06-11 1998-10-20 Tarr; George E. Peptide mass ladders generated using carbon disulfide
US6962818B2 (en) * 2000-10-19 2005-11-08 Target Discovery Mass defect labeling for the determination of oligomer sequences
US20040185448A1 (en) * 2003-03-20 2004-09-23 Viorica Lopez-Avila Methods and devices for performing matrix assisted laser desorption/lonization protocols
JP4832168B2 (ja) * 2006-06-02 2011-12-07 株式会社日立ハイテクノロジーズ デノボシーケンス解析方法，解析ソフト，解析ソフトを記憶した記憶媒体，試薬キット
GB2448562B (en) * 2006-10-24 2012-02-22 Bruker Daltonik Gmbh Top-down protein analysis in mass spectrometers with ion traps
GB0723183D0 (en) * 2007-11-23 2008-01-09 Micromass Ltd Mass spectrometer
ATE460666T1 (de) * 2008-01-15 2010-03-15 Univ Utrecht Holding Bv Verfahren zur bestimmung der aminosäurensequenz von peptiden
CN101298611B (zh) * 2008-06-12 2011-06-15 北京生命科学研究所 衣原体蛋白酶体样活性因子的活性片段及其表达方法
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
JP2011112562A (ja) * 2009-11-27 2011-06-09 Mitsubishi Chemicals Corp ペプチド末端の同定方法
US8455624B2 (en) * 2011-06-03 2013-06-04 University Of South Alabama Methods and compositions for detecting endometrial or ovarian cancer
JP6281349B2 (ja) 2014-03-19 2018-02-21 株式会社島津製作所 質量分析用ペプチド混合物試料の調製方法
WO2015185592A2 (en) * 2014-06-03 2015-12-10 Dsm Ip Assets B.V. Proline-specific endoprotease and use thereof
US9719078B1 (en) * 2014-06-15 2017-08-01 Darryl J. Pappin Proteases for the production of N-terminal argininyl- and lysinyl-peptides and methods of use in protein analysis
WO2016004233A2 (en) * 2014-07-01 2016-01-07 Expression Pathology, Inc. Srm assays to chemotherapy targets
SG11201800894SA (en) * 2015-08-03 2018-03-28 Shimadzu Corp Method for parallel quantification of protein variant
US11807671B2 (en) * 2016-11-16 2023-11-07 Auckland Uniservices Limited Methods for protein ligation and uses thereof
WO2019246140A1 (en) * 2018-06-18 2019-12-26 Emory University Parallel enzyme digestion for protein biomarker detection
KR20210045449A (ko) * 2018-08-17 2021-04-26 리제너론 파아마슈티컬스, 인크. 새로운 단백질 서열분석 방법

Also Published As

Publication number Publication date
BR112021002029A2 (pt) 2021-05-25
EP3837553A1 (en) 2021-06-23
US11047863B2 (en) 2021-06-29
SG11202100740YA (en) 2021-02-25
MX2021001923A (es) 2021-04-28
US20210278414A1 (en) 2021-09-09
EA202190550A1 (ru) 2021-04-23
JP2021534408A (ja) 2021-12-09
CN112601962A (zh) 2021-04-02
US20200057073A1 (en) 2020-02-20
KR20210045449A (ko) 2021-04-26
IL280628A (en) 2021-03-25
AU2019320814A1 (en) 2021-02-18
US12000840B2 (en) 2024-06-04
WO2020037205A1 (en) 2020-02-20
ZA202100694B (en) 2023-03-29

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