MX2021001923A - Metodos para la secuenciacion de proteinas de novo. - Google Patents
Metodos para la secuenciacion de proteinas de novo.Info
- Publication number
- MX2021001923A MX2021001923A MX2021001923A MX2021001923A MX2021001923A MX 2021001923 A MX2021001923 A MX 2021001923A MX 2021001923 A MX2021001923 A MX 2021001923A MX 2021001923 A MX2021001923 A MX 2021001923A MX 2021001923 A MX2021001923 A MX 2021001923A
- Authority
- MX
- Mexico
- Prior art keywords
- mass
- amino acid
- sets
- peptide
- peptide ions
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
- G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
- H01J49/28—Static spectrometers
- H01J49/30—Static spectrometers using magnetic analysers, e.g. Dempster spectrometer
- H01J49/305—Static spectrometers using magnetic analysers, e.g. Dempster spectrometer with several sectors in tandem
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Medical Informatics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Theoretical Computer Science (AREA)
- Evolutionary Biology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Epidemiology (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Artificial Intelligence (AREA)
- Evolutionary Computation (AREA)
- Signal Processing (AREA)
- Public Health (AREA)
- Software Systems (AREA)
- Data Mining & Analysis (AREA)
- Optics & Photonics (AREA)
- Databases & Information Systems (AREA)
Abstract
Un método para determinar una secuencia de aminoácidos de un polipéptido, que incluye: poner en contacto una primera y segunda muestras que contienen el polipéptido con una primera proteasa (por ejemplo, tripsina) y una segunda proteasa (por ejemplo, Tryp-N), respectivamente, para producir un primer y segundo conjuntos de fragmentos de péptidos digeridos; fragmentar los conjuntos de fragmentos de péptidos digeridos para producir conjuntos de iones de péptidos fragmentados; seleccionar pares de iones de péptidos de los conjuntos de iones de péptidos que difieren en masa en una masa de un residuo de aminoácido arginina o lisina; asignar un tipo de ion (ion de péptido de extremo N terminal o de extremo C terminal) a los pares seleccionados de iones de péptidos de los dos conjuntos; seleccionar una escalera de masa de los iones de péptidos del mismo tipo en cualquier conjunto de iones de péptidos con masa incremental por la masa de uno o más residuos de aminoácidos, y ensamblar los residuos de aminoácidos identificados de la escalera de masa para determinar la secuencia de aminoácidos del polipéptido de interés.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862719292P | 2018-08-17 | 2018-08-17 | |
| PCT/US2019/046821 WO2020037205A1 (en) | 2018-08-17 | 2019-08-16 | Methods for de novo protein sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2021001923A true MX2021001923A (es) | 2021-04-28 |
Family
ID=67851219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2021001923A MX2021001923A (es) | 2018-08-17 | 2019-08-16 | Metodos para la secuenciacion de proteinas de novo. |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US11047863B2 (es) |
| EP (1) | EP3837553A1 (es) |
| JP (1) | JP2021534408A (es) |
| KR (1) | KR20210045449A (es) |
| CN (1) | CN112601962A (es) |
| AU (1) | AU2019320814A1 (es) |
| BR (1) | BR112021002029A2 (es) |
| CA (1) | CA3107963A1 (es) |
| EA (1) | EA202190550A1 (es) |
| IL (1) | IL280628A (es) |
| MX (1) | MX2021001923A (es) |
| SG (1) | SG11202100740YA (es) |
| WO (1) | WO2020037205A1 (es) |
| ZA (1) | ZA202100694B (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EA202190550A1 (ru) * | 2018-08-17 | 2021-04-23 | Ридженерон Фармасьютикалз, Инк. | Способы секвенирования белков de novo |
| CA3117889A1 (en) | 2018-11-15 | 2020-05-22 | Quantum-Si Incorporated | Methods and compositions for protein sequencing |
| BR112022007937A2 (pt) * | 2019-10-28 | 2022-08-30 | Quantum Si Inc | Métodos de sequenciamento e reconstrução de um único polipeptídeo |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5824556A (en) * | 1997-06-11 | 1998-10-20 | Tarr; George E. | Peptide mass ladders generated using carbon disulfide |
| ES2385335T3 (es) * | 2000-10-19 | 2012-07-23 | Target Discovery, Inc. | Marcaje de defecto de masa para la determinación de secuencias de oligómeros |
| US20040185448A1 (en) * | 2003-03-20 | 2004-09-23 | Viorica Lopez-Avila | Methods and devices for performing matrix assisted laser desorption/lonization protocols |
| JP4832168B2 (ja) * | 2006-06-02 | 2011-12-07 | 株式会社日立ハイテクノロジーズ | デノボシーケンス解析方法，解析ソフト，解析ソフトを記憶した記憶媒体，試薬キット |
| GB2448562B (en) * | 2006-10-24 | 2012-02-22 | Bruker Daltonik Gmbh | Top-down protein analysis in mass spectrometers with ion traps |
| GB0723183D0 (en) * | 2007-11-23 | 2008-01-09 | Micromass Ltd | Mass spectrometer |
| DE602008000796D1 (de) * | 2008-01-15 | 2010-04-22 | Univ Utrecht Holding Bv | Verfahren zur Bestimmung der Aminosäurensequenz von Peptiden |
| CN101298611B (zh) * | 2008-06-12 | 2011-06-15 | 北京生命科学研究所 | 衣原体蛋白酶体样活性因子的活性片段及其表达方法 |
| RU2522002C2 (ru) | 2009-06-26 | 2014-07-10 | Ридженерон Фармасьютикалз, Инк. | Легковыделяемые биспецифические антитела с природным иммуноглобулиновым форматом |
| JP2011112562A (ja) * | 2009-11-27 | 2011-06-09 | Mitsubishi Chemicals Corp | ペプチド末端の同定方法 |
| EP2715367A2 (en) * | 2011-06-03 | 2014-04-09 | University of South Alabama | Methods and compositions for detecting endometrial or ovarian cancer |
| JP6281349B2 (ja) | 2014-03-19 | 2018-02-21 | 株式会社島津製作所 | 質量分析用ペプチド混合物試料の調製方法 |
| ES2718071T3 (es) * | 2014-06-03 | 2019-06-27 | Dsm Ip Assets Bv | Endoproteasa específica para prolina y su uso |
| US9719078B1 (en) | 2014-06-15 | 2017-08-01 | Darryl J. Pappin | Proteases for the production of N-terminal argininyl- and lysinyl-peptides and methods of use in protein analysis |
| EP3164708A4 (en) * | 2014-07-01 | 2018-03-14 | Expression Pathology, Inc. | Srm assays to chemotherapy targets |
| SG11201800894SA (en) * | 2015-08-03 | 2018-03-28 | Shimadzu Corp | Method for parallel quantification of protein variant |
| WO2018093274A1 (en) * | 2016-11-16 | 2018-05-24 | Auckland Uniservices Limited | Methods for protein ligation and uses thereof |
| EA202190550A1 (ru) * | 2018-08-17 | 2021-04-23 | Ридженерон Фармасьютикалз, Инк. | Способы секвенирования белков de novo |
-
2019
- 2019-08-16 EA EA202190550A patent/EA202190550A1/ru unknown
- 2019-08-16 BR BR112021002029-2A patent/BR112021002029A2/pt active Search and Examination
- 2019-08-16 CN CN201980052998.0A patent/CN112601962A/zh active Pending
- 2019-08-16 EP EP19763132.8A patent/EP3837553A1/en active Pending
- 2019-08-16 CA CA3107963A patent/CA3107963A1/en active Pending
- 2019-08-16 AU AU2019320814A patent/AU2019320814A1/en active Pending
- 2019-08-16 SG SG11202100740YA patent/SG11202100740YA/en unknown
- 2019-08-16 MX MX2021001923A patent/MX2021001923A/es unknown
- 2019-08-16 WO PCT/US2019/046821 patent/WO2020037205A1/en active Application Filing
- 2019-08-16 US US16/542,788 patent/US11047863B2/en active Active
- 2019-08-16 KR KR1020217007865A patent/KR20210045449A/ko not_active Application Discontinuation
- 2019-08-16 JP JP2021507942A patent/JP2021534408A/ja active Pending
-
2021
- 2021-02-01 ZA ZA2021/00694A patent/ZA202100694B/en unknown
- 2021-02-03 IL IL280628A patent/IL280628A/en unknown
- 2021-05-24 US US17/327,889 patent/US12000840B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020037205A1 (en) | 2020-02-20 |
| US11047863B2 (en) | 2021-06-29 |
| US20200057073A1 (en) | 2020-02-20 |
| AU2019320814A1 (en) | 2021-02-18 |
| EA202190550A1 (ru) | 2021-04-23 |
| BR112021002029A2 (pt) | 2021-05-25 |
| CA3107963A1 (en) | 2020-02-20 |
| ZA202100694B (en) | 2023-03-29 |
| US12000840B2 (en) | 2024-06-04 |
| EP3837553A1 (en) | 2021-06-23 |
| JP2021534408A (ja) | 2021-12-09 |
| US20210278414A1 (en) | 2021-09-09 |
| SG11202100740YA (en) | 2021-02-25 |
| CN112601962A (zh) | 2021-04-02 |
| KR20210045449A (ko) | 2021-04-26 |
| IL280628A (en) | 2021-03-25 |
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