BR112022007937A2 - Métodos de sequenciamento e reconstrução de um único polipeptídeo - Google Patents

Métodos de sequenciamento e reconstrução de um único polipeptídeo

Info

Publication number
BR112022007937A2
BR112022007937A2 BR112022007937A BR112022007937A BR112022007937A2 BR 112022007937 A2 BR112022007937 A2 BR 112022007937A2 BR 112022007937 A BR112022007937 A BR 112022007937A BR 112022007937 A BR112022007937 A BR 112022007937A BR 112022007937 A2 BR112022007937 A2 BR 112022007937A2
Authority
BR
Brazil
Prior art keywords
sequencing
methods
polypeptide
fragments
reconstruction
Prior art date
Application number
BR112022007937A
Other languages
English (en)
Inventor
Dyer Matthew
Reed Brian
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of BR112022007937A2 publication Critical patent/BR112022007937A2/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

MÉTODOS DE SEQUENCIAMENTO E RECONSTRUÇÃO DE UM ÚNICO POLIPEPTÍDEO. Métodos de sequenciamento de um único polipeptídeo. Os métodos compreendem fornecer uma amostra enriquecida que compreende uma população de polipeptídeos; dividir a amostra enriquecida em duas ou mais subamostras; contatar cada uma de pelo menos duas das suba-mostras com um agente modificador diferente, em que o agente modificador compreende um agente de clivagem, por exemplo, uma exopeptidase, deste modo, gerando fragmentos polipeptídicos que têm uma combinação de padrões de clivagem; e sequenciar, em paralelo, os fragmentos polipeptídicos, deste modo, determinando as sequências de aminoácidos dos fragmentos polipeptídicos. Os fragmentos podem ser alinhados para reconstruir a sequência polipeptídica. Também é fornecido no presente documento um kit que compreende uma pluralidade de moléculas de enriquecimento, por exemplo, anticorpos, aptâmeros ou enzimas, e um dispositivo de preparação de amostras que compreendem códigos de barras e sondas de captura.
BR112022007937A 2019-10-28 2020-10-28 Métodos de sequenciamento e reconstrução de um único polipeptídeo BR112022007937A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962927005P 2019-10-28 2019-10-28
US201962940968P 2019-11-27 2019-11-27
PCT/US2020/057669 WO2021086918A1 (en) 2019-10-28 2020-10-28 Methods of single-polypeptide sequencing and reconstruction

Publications (1)

Publication Number Publication Date
BR112022007937A2 true BR112022007937A2 (pt) 2022-08-30

Family

ID=73476267

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022007937A BR112022007937A2 (pt) 2019-10-28 2020-10-28 Métodos de sequenciamento e reconstrução de um único polipeptídeo

Country Status (10)

Country Link
US (1) US20210148922A1 (pt)
EP (1) EP4051806A1 (pt)
JP (1) JP2023501932A (pt)
KR (1) KR20220108056A (pt)
CN (1) CN114929887A (pt)
AU (1) AU2020376814A1 (pt)
BR (1) BR112022007937A2 (pt)
CA (1) CA3159362A1 (pt)
MX (1) MX2022005094A (pt)
WO (1) WO2021086918A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201715684D0 (en) * 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
CA3117889A1 (en) 2018-11-15 2020-05-22 Quantum-Si Incorporated Methods and compositions for protein sequencing
WO2023038859A1 (en) * 2021-09-09 2023-03-16 Nautilus Biotechnology, Inc. Characterization and localization of protein modifications
US20230194539A1 (en) * 2021-12-22 2023-06-22 Trustees Of Boston University Bodipy-based dyes for digital spatial proteomics
WO2024086832A1 (en) * 2022-10-21 2024-04-25 Quantum-Si Incorporated Polypeptide cleaving reagents and uses thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
DE602008000796D1 (de) * 2008-01-15 2010-04-22 Univ Utrecht Holding Bv Verfahren zur Bestimmung der Aminosäurensequenz von Peptiden
CA2745197A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
WO2010065531A1 (en) * 2008-12-01 2010-06-10 Robi David Mitra Single molecule protein screening
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
US10545153B2 (en) * 2014-09-15 2020-01-28 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
WO2019089846A1 (en) * 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
CA3081441C (en) * 2017-10-31 2023-08-29 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
CN112513269A (zh) * 2018-07-12 2021-03-16 德克萨斯大学系统董事会 通过寡核苷酸进行分子邻域检测
SG11202100740YA (en) * 2018-08-17 2021-02-25 Regeneron Pharma Methods for de novo protein sequencing

Also Published As

Publication number Publication date
EP4051806A1 (en) 2022-09-07
MX2022005094A (es) 2022-08-15
WO2021086918A1 (en) 2021-05-06
CA3159362A1 (en) 2021-05-06
CN114929887A (zh) 2022-08-19
KR20220108056A (ko) 2022-08-02
JP2023501932A (ja) 2023-01-20
AU2020376814A1 (en) 2022-06-02
US20210148922A1 (en) 2021-05-20

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]