JP2021528975A5 - - Google Patents

Info

Publication number
JP2021528975A5
JP2021528975A5 JP2020572532A JP2020572532A JP2021528975A5 JP 2021528975 A5 JP2021528975 A5 JP 2021528975A5 JP 2020572532 A JP2020572532 A JP 2020572532A JP 2020572532 A JP2020572532 A JP 2020572532A JP 2021528975 A5 JP2021528975 A5 JP 2021528975A5
Authority
JP
Japan
Prior art keywords
nucleic acid
transposase
target nucleic
sample
crispr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2020572532A
Other languages
English (en)
Japanese (ja)
Other versions
JP2021528975A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2019/039195 external-priority patent/WO2020006049A1/en
Publication of JP2021528975A publication Critical patent/JP2021528975A/ja
Publication of JP2021528975A5 publication Critical patent/JP2021528975A5/ja
Pending legal-status Critical Current

Links

JP2020572532A 2018-06-26 2019-06-26 Crispr/cas及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法 Pending JP2021528975A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201862690160P 2018-06-26 2018-06-26
US62/690,160 2018-06-26
US201862767077P 2018-11-14 2018-11-14
US62/767,077 2018-11-14
PCT/US2019/039195 WO2020006049A1 (en) 2018-06-26 2019-06-26 Crispr/cas and transposase based amplification compositions, systems and methods

Publications (2)

Publication Number Publication Date
JP2021528975A JP2021528975A (ja) 2021-10-28
JP2021528975A5 true JP2021528975A5 (enExample) 2022-07-01

Family

ID=67263112

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020572532A Pending JP2021528975A (ja) 2018-06-26 2019-06-26 Crispr/cas及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法

Country Status (11)

Country Link
US (1) US12559786B2 (enExample)
EP (1) EP3814519A1 (enExample)
JP (1) JP2021528975A (enExample)
KR (1) KR20210024009A (enExample)
CN (1) CN112368396A (enExample)
AU (1) AU2019294690A1 (enExample)
BR (1) BR112020025319A2 (enExample)
CA (1) CA3101066A1 (enExample)
MX (1) MX2020013778A (enExample)
SG (1) SG11202011240YA (enExample)
WO (1) WO2020006049A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019010422A1 (en) 2017-07-07 2019-01-10 The Broad Institute, Inc. ANTIVIRAL THERAPY BASED ON THE CRISPR SYSTEM
US12171783B2 (en) 2017-11-13 2024-12-24 The Broad Institute, Inc. Methods and compositions for targeting developmental and oncogenic programs in H3K27M gliomas
SG11202011240YA (en) 2018-06-26 2020-12-30 Broad Inst Inc Crispr/cas and transposase based amplification compositions, systems and methods
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
WO2021046243A2 (en) 2019-09-03 2021-03-11 Myeloid Therapeutics, Inc. Methods and compositions for genomic integration
US20230340537A1 (en) 2020-01-17 2023-10-26 The Broad Institute, Inc. Small type ii-d cas proteins and methods of use thereof
EP4291678A1 (en) * 2021-02-09 2023-12-20 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
WO2022241029A1 (en) 2021-05-11 2022-11-17 Myeloid Therapeutics, Inc. Methods and compositions for genomic integration
WO2023283622A1 (en) 2021-07-08 2023-01-12 Montana State University Crispr-based programmable rna editing
US11814689B2 (en) 2021-07-21 2023-11-14 Montana State University Nucleic acid detection using type III CRISPR complex
KR102576803B1 (ko) * 2022-10-14 2023-09-12 주식회사 풀무원 CRISPR-Cas 기반의 리스테리아 모노사이토제네스 검출용 조성물 및 이를 이용한 리스테리아 모노사이토제네스 검출방법
US12435357B2 (en) 2022-12-22 2025-10-07 Kanso Diagnostics Ltd. Diagnostic device for detecting a target nucleic acid molecule in a biological sample
WO2024238563A2 (en) * 2023-05-15 2024-11-21 Vedabio, Inc. Arrayed detection of target molecules via crispr cascade reactions

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005038029A2 (en) 2003-10-10 2005-04-28 Promega Corporation Luciferase biosensor
US9193959B2 (en) 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
CN116622704A (zh) 2012-07-25 2023-08-22 布罗德研究所有限公司 可诱导的dna结合蛋白和基因组干扰工具及其应用
MX380562B (es) 2012-12-12 2025-03-12 Broad Inst Inc Modificaciones de sistemas, métodos y composiciones guía optimizadas para la manipulación de secuencias.
WO2014093709A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
SG10201707569YA (en) 2012-12-12 2017-10-30 Broad Inst Inc Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications
EP3064585B1 (en) 2012-12-12 2020-02-05 The Broad Institute, Inc. Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
WO2014093694A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
CN105209621B (zh) 2012-12-12 2021-05-25 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
KR102955556B1 (ko) 2012-12-12 2026-04-22 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물
PT2931898E (pt) 2012-12-12 2016-06-16 Harvard College Manipulação e otimização de sistemas, métodos e composições para manipulação de sequências com domínios funcionais
US11332719B2 (en) 2013-03-15 2022-05-17 The Broad Institute, Inc. Recombinant virus and preparations thereof
ES2777217T3 (es) 2013-06-17 2020-08-04 Broad Inst Inc Suministro, modificación y optimización de sistemas de guía en tándem, métodos y composiciones para la manipulación de secuencias
EP3011033B1 (en) 2013-06-17 2020-02-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
SG11201510327TA (en) 2013-06-17 2016-01-28 Broad Inst Inc Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells
KR20250012194A (ko) 2013-06-17 2025-01-23 더 브로드 인스티튜트, 인코퍼레이티드 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용
WO2014204725A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
WO2014204723A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Oncogenic models based on delivery and use of the crispr-cas systems, vectors and compositions
JP6738728B2 (ja) 2013-06-17 2020-08-19 ザ・ブロード・インスティテュート・インコーポレイテッド 肝臓ターゲティングおよび治療のためのCRISPR−Cas系、ベクターおよび組成物の送達および使用
EP3058091B1 (en) 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
CN111218447B (zh) 2013-11-07 2024-10-11 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
CN105899658B (zh) 2013-12-12 2020-02-18 布罗德研究所有限公司 针对hbv和病毒性疾病以及障碍的crispr-cas系统和组合物的递送、用途和治疗应用
WO2015089419A2 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
CA2932439A1 (en) 2013-12-12 2015-06-18 The Broad Institute, Inc. Crispr-cas systems and methods for altering expression of gene products, structural information and inducible modular cas enzymes
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
KR20160097338A (ko) 2013-12-12 2016-08-17 더 브로드 인스티튜트, 인코퍼레이티드 뉴클레오티드 반복 장애에서의 crispr-cas 시스템의 조성물 및 방법 및 용도
JP6793547B2 (ja) 2013-12-12 2020-12-02 ザ・ブロード・インスティテュート・インコーポレイテッド 最適化機能CRISPR−Cas系による配列操作のための系、方法および組成物
WO2015089473A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
CN111206032B (zh) 2013-12-12 2024-07-19 布罗德研究所有限公司 用于基因组编辑的crispr-cas系统和组合物的递送、用途和治疗应用
CN105463066B (zh) * 2014-09-04 2019-05-17 中国科学院北京基因组研究所 一种dna扩增方法
EP3985115A1 (en) 2014-12-12 2022-04-20 The Broad Institute, Inc. Protected guide rnas (pgrnas)
EP3230452B1 (en) 2014-12-12 2025-06-11 The Broad Institute, Inc. Dead guides for crispr transcription factors
WO2016094874A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Escorted and functionalized guides for crispr-cas systems
PT3234187T (pt) * 2014-12-15 2021-05-26 Illumina Inc Método para colocação de moleculas de alvo unico num substrato
WO2016106244A1 (en) 2014-12-24 2016-06-30 The Broad Institute Inc. Crispr having or associated with destabilization domains
WO2016138292A1 (en) * 2015-02-25 2016-09-01 Igenomx International Genomics Corporation Methods and compositions for in silico long read sequencing
US11155822B2 (en) * 2015-05-14 2021-10-26 Universidad De Los Andes Transposon that promotes functional DNA expression in episomal DNAs and method to enhance DNA transcription during functional analysis of metagenomic libraries
US10894980B2 (en) * 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
EP3445853A1 (en) * 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
CA3026110A1 (en) 2016-04-19 2017-11-02 The Broad Institute, Inc. Novel crispr enzymes and systems
CN105861552B (zh) 2016-04-25 2019-10-11 西北农林科技大学 一种T7 RNA聚合酶介导的CRISPR/Cas9基因编辑系统的构建方法
CN105950639A (zh) * 2016-05-04 2016-09-21 广州美格生物科技有限公司 金黄色葡萄球菌CRISPR/Cas9系统的制备及其在构建小鼠模型中的应用
AU2017283713B2 (en) 2016-06-17 2021-04-08 Massachusetts Institute Of Technology Type VI CRISPR orthologs and systems
KR102993694B1 (ko) * 2016-12-09 2026-07-22 더 브로드 인스티튜트, 인코퍼레이티드 Crispr 이펙터 시스템 기반 진단
CN111108220B (zh) 2017-03-15 2024-11-19 博德研究所 用于病毒检测的基于crispr效应系统的诊断
US10844372B2 (en) * 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN107937328B (zh) * 2017-11-30 2018-09-14 中央民族大学 基于细胞的比较器及应用与细胞计算机
SG11202011240YA (en) 2018-06-26 2020-12-30 Broad Inst Inc Crispr/cas and transposase based amplification compositions, systems and methods

Similar Documents

Publication Publication Date Title
US11866773B2 (en) Isolated oligonucleotides containing modified nucleotides
CN119913238A (zh) 用于即时核酸检测的组合物和方法
CN110982940A (zh) 基于熔解曲线的麻疹病毒、风疹病毒和腮腺炎病毒核酸检测的组合物及试剂盒
CN104404162A (zh) 采用引物关联通用探针检测多重基因或不同靶标的实时荧光pcr方法
CN113278738A (zh) 用于副流感病毒分型检测的引物探针组合物及实时荧光定量pcr试剂盒
Thaitrong et al. Integrated capillary electrophoresis microsystem for multiplex analysis of human respiratory viruses
EP4265741A1 (en) Multiplexable crispr-cas9-based virus detection method
CN224015607U (zh) 一种荧光检测用的盖体及反应管
RU2020141821A (ru) КОМПОЗИЦИИ ДЛЯ АМПЛИФИКАЦИИ, СИСТЕМЫ И СПОСОБЫ НА ОСНОВЕ CRISPR/Cas И ТРАНСПОЗАЗЫ
CN223522539U (zh) 一种反应管管体用部件及一种反应管
CN121227907A (zh) 一种基于Cas12a变构调节实现肺炎支原体便携式超敏检测试剂盒及应用
CN120758604A (zh) 一种高效核糖核酸酶双引物等温扩增方法
HK1210228B (en) Compositions and methods for quantifying a nucleic acid sequence in a sample