JP2021515910A - 大型試料を走査するための光学装置、光学モジュールおよび顕微鏡 - Google Patents
大型試料を走査するための光学装置、光学モジュールおよび顕微鏡 Download PDFInfo
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Abstract
Description
3 光学照明アセンブリ
5 光学検出アセンブリ
7 取付け要素
8a 入力側光学断面
8b 出力側光学断面
9 レンズアセンブリ
10 像側主平面
11 レンズ
13 ハウジング
15 照明光
17 試料側
19 機器側
21 照明経路
21a 近軸照明経路
21b 周辺照明経路
21u 不変の照明経路
23 試料ボリューム
25 試料
27 散乱光および/または蛍光
29 検出経路
31 境界面
31a 側面
33 偏向ミラー
35 ダイクロイックミラー
37 顕微鏡
39 共焦点顕微鏡
41 光学系
43 シリンドリカルレンズ
45 光シート
47 拡大図
49 検出器系
51 伝搬方向
53 幅方向
55 厚さ
57 厚さ方向
59 伝送経路
59a 近軸伝送経路
59b 周辺伝送経路
61 入力経路
63 出力経路
65 フランジ領域
67 対物レンズ
68 液浸光学系
69 液浸対物レンズ
71 前面
73 液浸媒体
73a 液浸媒体の屈折率
75 上面
77 振動
79 第1の端部
81 第2の端部
83 試料ホルダー
85 走査方向
87 走査ミラー
89 軸線
91 仮想光シート
93 走査運動
95 光学モジュール
97 収容装置
99 入射もしくは出射開口部
101 波長選択光学素子
102 透過および/または反射特性
103 偏向素子
103a 偏向素子の屈折率
105 透過光
107 調整角
109 構成
109a 近軸照明構成
109b 周辺照明構成
111 光軸
113 視射角
115 全反射
117 反射層
119 底面
121 固定要素
123 ボリューム体
Claims (15)
- 試料ボリューム(23)内に配置された試料(25)を照明光(15)で照明し、前記試料(25)からの散乱光および/または蛍光(27)を検出するための光学装置(1)であって、
前記光学装置(1)は、前記照明光(15)を照明経路(21)に沿って前記試料ボリューム(23)内に伝送するための光学照明アセンブリ(3)と、前記散乱光および/または前記蛍光(27)を検出経路(29)に沿って前記試料ボリューム(23)から収集して転送するための光学検出アセンブリ(5)と、を含んでいる、光学装置(1)において、
前記光学装置(1)は、前記照明経路(21)および/または前記検出経路(29)が少なくとも部分的に延びた少なくとも1つの取付け要素(7)を備える、
光学装置(1)。 - 前記取付け要素(7)は、入力側光学断面(8a)および出力側光学断面(8b)を有し、前記入力側光学断面(8a)は、前記出力側光学断面(8b)より大きい、
請求項1記載の光学装置(1)。 - 前記取付け要素(7)は、前記光学照明アセンブリ(3)および/または前記光学検出アセンブリ(5)に固定されている、
請求項1または2記載の光学装置(1)。 - 少なくとも前記光学検出アセンブリ(5)は、液浸光学系(68)である、
請求項1から3までのいずれか1項記載の光学装置(1)。 - 前記取付け要素(7)は、少なくとも2つの境界面(31)を有し、前記境界面(31)は、前記照明経路(21)および/または前記検出経路(29)に対して実質的に垂直に配向されている、
請求項1から4までのいずれか1項記載の光学装置(1)。 - 前記取付け要素(7)は、前記照明光(15)および/または前記散乱光および/または前記蛍光(27)を偏向させる偏向素子(103)である、
請求項1から5までのいずれか1項記載の光学装置(1)。 - 前記偏向素子(103)において、前記照明光(15)および/または前記散乱光および/または前記蛍光(27)用の伝送経路(59)は、前記偏向素子(103)の側面(31a)に対して鋭角な視射角(113)で配向されており、前記側面(31a)および透過光(105)について、全反射(115)用の条件が満たされている、
請求項6記載の光学装置(1)。 - 前記側面(31a)は、前記照明光(15)および前記蛍光(27)用の反射層(117)を有する、
請求項7記載の光学装置(1)。 - 前記取付け要素(7)は、カップ形状に形成されており、前記光学照明アセンブリ(3)に向かって開口している、
請求項1から5までのいずれか1項記載の光学装置(1)。 - 前記取付け要素(7)は、試料ボリューム(23)に向かって透過要素を有する、
請求項9記載の光学装置(1)。 - 前記取付け要素(7)は、充填されている、
請求項9または10記載の光学装置(1)。 - 光学装置(1)を含む光学モジュール(95)において、
前記光学装置(1)は、請求項1から11までのいずれか1項記載の光学装置(1)である、
光学モジュール(95)。 - 照明光(15)ならびに散乱光および/または蛍光(27)用に、共有の入射もしくは出射開口部(99)が、前記光学モジュール(95)に設けられている、
請求項12記載の光学モジュール(95)。 - 少なくとも1つの波長選択光学素子(101)が設けられており、前記波長選択光学素子(101)は、前記散乱光および/または前記蛍光(27)用の透過および/または反射特性(102)とは異なる、前記照明光(15)用の異なる透過および/または反射特性(102)を有する、
請求項12または13記載の光学モジュール(95)。 - 光学モジュール(95)用の機械的な収容装置(97)を含んだ顕微鏡(37)、特に共焦点顕微鏡(39)において、
請求項12から14までのいずれか1項記載の光学モジュール(95)は、前記収容装置(97)内に収容されている、
顕微鏡(37)、特に共焦点顕微鏡(39)。
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DE102018203247.5 | 2018-03-05 | ||
DE102018203247.5A DE102018203247A1 (de) | 2018-03-05 | 2018-03-05 | Optische Vorrichtung, optisches Modul und Mikroskop zum Abtasten großer Proben |
PCT/EP2019/054146 WO2019170413A1 (de) | 2018-03-05 | 2019-02-20 | Optische vorrichtung, optisches modul und mikroskop zum abtasten grosser proben |
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US (1) | US12019227B2 (ja) |
EP (1) | EP3762707A1 (ja) |
JP (1) | JP2021515910A (ja) |
CN (1) | CN111819434A (ja) |
DE (1) | DE102018203247A1 (ja) |
WO (1) | WO2019170413A1 (ja) |
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DE102018222876A1 (de) * | 2018-12-21 | 2020-06-25 | Leica Microsystems Cms Gmbh | Mikroskop und Verfahren zur mikroskopischen Untersuchung großer Proben |
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JP2018032009A (ja) * | 2016-08-18 | 2018-03-01 | オリンパス株式会社 | 顕微鏡 |
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JP2004279910A (ja) * | 2003-03-18 | 2004-10-07 | Tsutomu Masujima | 生体関連物質を観察する装置 |
JP2017522603A (ja) * | 2014-07-22 | 2017-08-10 | ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツングLeica Microsystems CMS GmbH | 試料を顕微鏡検査する方法及び装置 |
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CN111819434A (zh) | 2020-10-23 |
DE102018203247A1 (de) | 2019-09-05 |
US20210063714A1 (en) | 2021-03-04 |
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