JP2021006028A5 - - Google Patents

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JP2021006028A5
JP2021006028A5 JP2020135592A JP2020135592A JP2021006028A5 JP 2021006028 A5 JP2021006028 A5 JP 2021006028A5 JP 2020135592 A JP2020135592 A JP 2020135592A JP 2020135592 A JP2020135592 A JP 2020135592A JP 2021006028 A5 JP2021006028 A5 JP 2021006028A5
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JP2021006028A (ja
JP7091400B2 (ja
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JP2020135592A 2013-12-02 2020-08-11 核酸の多重検出 Active JP7091400B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1321196.6 2013-12-02
GB1321196.6A GB2520765A (en) 2013-12-02 2013-12-02 Multiplex detection of nucleic acids

Related Parent Applications (1)

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JP2016535001A Division JP6749236B2 (ja) 2013-12-02 2014-11-26 核酸の多重検出

Publications (3)

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JP2021006028A JP2021006028A (ja) 2021-01-21
JP2021006028A5 true JP2021006028A5 (enExample) 2021-04-22
JP7091400B2 JP7091400B2 (ja) 2022-06-27

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JP2016535001A Active JP6749236B2 (ja) 2013-12-02 2014-11-26 核酸の多重検出
JP2020135592A Active JP7091400B2 (ja) 2013-12-02 2020-08-11 核酸の多重検出

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JP2016535001A Active JP6749236B2 (ja) 2013-12-02 2014-11-26 核酸の多重検出

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US (2) US10526643B2 (enExample)
EP (2) EP3757228B1 (enExample)
JP (2) JP6749236B2 (enExample)
KR (2) KR102307230B1 (enExample)
CN (1) CN105934523B (enExample)
AU (1) AU2014358862B2 (enExample)
BR (1) BR112016012319B1 (enExample)
CA (1) CA2931015C (enExample)
ES (2) ES2807614T3 (enExample)
GB (1) GB2520765A (enExample)
RU (1) RU2733887C2 (enExample)
WO (1) WO2015083002A2 (enExample)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
GB2520763A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
JP6976167B2 (ja) 2014-06-13 2021-12-08 キュー−リネア エービー 微生物の検出及び特徴付け方法
EP4282977B1 (en) 2015-04-10 2024-06-05 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
GB201507376D0 (en) 2015-04-30 2015-06-17 Vanadis Diagnostics Ab Use of a porous transparent capillary membrane for counting rolling circle amplification products
RU2753883C2 (ru) * 2015-09-18 2021-08-24 Ванадис Дайэгностикс Набор зондов для анализа образцов днк и способы их использования
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
BR112019007865A2 (pt) * 2016-10-27 2019-07-02 Vanadis Diagnostics método para processamento de produtos de amplificação do círculo rolante
GB201621514D0 (en) 2016-12-16 2017-02-01 Q-Linea Ab Padlock probe detection method
CN106929593B (zh) * 2017-04-27 2021-04-30 华侨大学 一种原位核酸检测方法
CN109252224A (zh) * 2017-07-14 2019-01-22 深圳华大基因股份有限公司 一种环状探针和基于环状探针捕获的测序文库构建方法
US11117128B2 (en) 2017-08-25 2021-09-14 Vanadis Diagnostics Ab Filtration device
CA3095292A1 (en) 2018-04-02 2019-10-10 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
US12203129B2 (en) * 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US12497649B2 (en) 2018-12-05 2025-12-16 Qingdao MGI Tech Co., Ltd Rolling circle amplification method, method for preparing sequencing library, and DNA nanosphere prepared therefrom
WO2020123311A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN112029841B (zh) * 2019-06-03 2024-02-09 香港中文大学 定量端粒长度和基因组基序的方法
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
DK3891300T3 (da) 2019-12-23 2023-05-22 10X Genomics Inc Fremgangsmåder til rumlig analyse ved anvendelse af rna-template-ligering
US12241890B2 (en) 2019-12-23 2025-03-04 10X Genomics, Inc. Methods for generating barcoded nucleic acid molecules using fixed cells
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
ES3014967T3 (en) 2020-04-22 2025-04-28 10X Genomics Inc Methods for spatial analysis using targeted rna depletion
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US20230220474A1 (en) * 2020-06-10 2023-07-13 Bonadea Diagnostics, Llc Sequence conversion reaction
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
EP4214243A4 (en) 2020-09-21 2025-06-18 Enumera Molecular, Inc. COMPOSITIONS AND METHODS FOR ISOLATING CELL-FREE DNA
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
JP2024504247A (ja) 2020-12-11 2024-01-31 エニュメラ・モレキュラー・インコーポレイテッド 画像処理のための方法及びシステム
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023287765A1 (en) * 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for spatial analysis using targeted probe silencing
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
CN113801923A (zh) * 2021-10-20 2021-12-17 南京鼓楼医院 一种可直接用于血清标志物检测的数字滚环扩增检测方法
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
CN116625999B (zh) * 2023-05-17 2023-11-28 中国科学院苏州生物医学工程技术研究所 一种评估零模波导孔有效载样的方法及其应用
WO2025194315A1 (zh) * 2024-03-18 2025-09-25 深圳华大智造科技股份有限公司 核酸检测方法及其应用

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
AU3601599A (en) 1998-03-25 1999-10-18 Ulf Landegren Rolling circle replication of padlock probes
WO2000004193A1 (en) * 1998-07-20 2000-01-27 Yale University Method for detecting nucleic acids using target-mediated ligation of bipartite primers
WO2001094625A2 (en) 2000-06-06 2001-12-13 Tm Bioscience Corporation Capture moieties for nucleic acids and uses thereof
ATE380883T1 (de) 2000-10-24 2007-12-15 Univ Leland Stanford Junior Direkte multiplex charakterisierung von genomischer dna
GB2378245A (en) 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
CA2467460A1 (en) 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
US20040166514A1 (en) * 2002-11-19 2004-08-26 Singulex, Inc. Detection of target molecules through interaction with probes
WO2005047547A1 (en) 2003-11-04 2005-05-26 Applera Corporation Concatameric ligation products: compositions, methods and kits for same
SE0401270D0 (sv) 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
CA2614118C (en) 2005-07-04 2013-11-26 Erasmus University Medical Center 4c
US20070087355A1 (en) * 2005-10-14 2007-04-19 Barrett Michael T Comparative genomic hybridization assays and compositions for practicing the same
US8038595B2 (en) 2006-01-25 2011-10-18 Beth Israel Deaconess Medical Center Devices and methods for tissue transplant and regeneration
WO2008033442A2 (en) 2006-09-12 2008-03-20 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
US20100291636A1 (en) 2007-06-11 2010-11-18 Olink Genomics Ab Method for introducing common and/or individual sequence elements in a target nucleic acid molecule
US20090061424A1 (en) 2007-08-30 2009-03-05 Sigma-Aldrich Company Universal ligation array for analyzing gene expression or genomic variations
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
WO2011068909A2 (en) * 2009-12-01 2011-06-09 Brigham And Women's Hospital, Inc. Aptamer cell compositions
GB0921264D0 (en) 2009-12-03 2010-01-20 Olink Genomics Ab Method for amplification of target nucleic acid
US20140186827A1 (en) 2010-05-14 2014-07-03 Fluidigm, Inc. Assays for the detection of genotype, mutations, and/or aneuploidy
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11270781B2 (en) * 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
GB2492042B (en) 2011-05-11 2020-04-15 Agilent Technologies Inc Selector probes for nucleic acid analysis comprising at each end a non-adjacent target specific region
WO2013079649A1 (en) 2011-12-02 2013-06-06 Qiagen Gmbh Method and kit for characterizing rna in a composition
LT2805280T (lt) 2012-01-20 2022-12-27 Sequenom, Inc. Diagnostikos būdai, kurie atsižvelgia į eksperimentines sąlygas
US9892230B2 (en) * 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
EP3178945B1 (en) * 2012-04-06 2018-10-17 The Chinese University Of Hong Kong Method of analyzing a biological sample from a female subject pregnant with a fetus
EP2653559A1 (en) 2012-04-20 2013-10-23 Samsung Electronics Co., Ltd Polynucleotide and use thereof
CA2901138A1 (en) * 2013-03-12 2014-10-09 Counsyl, Inc. Systems and methods for prenatal genetic analysis
GB2520763A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
GB2520765A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Multiplex detection of nucleic acids
US10174383B2 (en) * 2014-08-13 2019-01-08 Vanadis Diagnostics Method of estimating the amount of a methylated locus in a sample

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