JP2021000139A5 - - Google Patents
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- Publication number
- JP2021000139A5 JP2021000139A5 JP2020168502A JP2020168502A JP2021000139A5 JP 2021000139 A5 JP2021000139 A5 JP 2021000139A5 JP 2020168502 A JP2020168502 A JP 2020168502A JP 2020168502 A JP2020168502 A JP 2020168502A JP 2021000139 A5 JP2021000139 A5 JP 2021000139A5
- Authority
- JP
- Japan
- Prior art keywords
- group
- deoxynucleoside triphosphate
- disulfide
- detectable label
- nucleobase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562251884P | 2015-11-06 | 2015-11-06 | |
| US62/251,884 | 2015-11-06 | ||
| US201662327555P | 2016-04-26 | 2016-04-26 | |
| US62/327,555 | 2016-04-26 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018522968A Division JP6775014B2 (ja) | 2015-11-06 | 2016-11-04 | ヌクレオチド類似体 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021000139A JP2021000139A (ja) | 2021-01-07 |
| JP2021000139A5 true JP2021000139A5 (https=) | 2021-02-18 |
| JP7119041B2 JP7119041B2 (ja) | 2022-08-16 |
Family
ID=58663032
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018522968A Expired - Fee Related JP6775014B2 (ja) | 2015-11-06 | 2016-11-04 | ヌクレオチド類似体 |
| JP2020168502A Expired - Fee Related JP7119041B2 (ja) | 2015-11-06 | 2020-10-05 | Ｄｎａ配列において標識されたヌクレオチドを検出する方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018522968A Expired - Fee Related JP6775014B2 (ja) | 2015-11-06 | 2016-11-04 | ヌクレオチド類似体 |
Country Status (9)
| Country | Link |
|---|---|
| US (7) | US10273539B2 (https=) |
| EP (1) | EP3370731A4 (https=) |
| JP (2) | JP6775014B2 (https=) |
| KR (2) | KR102298737B1 (https=) |
| CN (1) | CN109476694B (https=) |
| AU (1) | AU2016349457B2 (https=) |
| CA (2) | CA3158679A1 (https=) |
| TW (3) | TWI639614B (https=) |
| WO (1) | WO2017079498A2 (https=) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108779138B (zh) * | 2015-09-28 | 2022-06-17 | 哥伦比亚大学董事会 | 用作dna合成测序的可逆终止物的基于新的二硫键接头的核苷酸的设计与合成 |
| TWI639614B (zh) * | 2015-11-06 | 2018-11-01 | 美商齊亞雋科學公司 | 核苷酸類似物 |
| WO2017205336A1 (en) | 2016-05-23 | 2017-11-30 | The Trustees Of Columbia University In The City Of New York | Nucleotide derivatives and methods of use thereof |
| CN115141880A (zh) * | 2017-01-10 | 2022-10-04 | 深圳华大智造科技股份有限公司 | 一种提高核酸聚合测序质量的方法、反应体系和试剂盒 |
| EP3580351A1 (en) | 2017-02-13 | 2019-12-18 | Qiagen Sciences, LLC | Polymerase enzyme from 9°n |
| EP3596099B1 (en) | 2017-03-06 | 2024-07-24 | Singular Genomics Systems, Inc. | Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps |
| WO2018183538A1 (en) | 2017-03-28 | 2018-10-04 | The Trustees Of Columbia University In The City Of New York | 3'-o-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for dna sequencing by synthesis |
| EP3733683A4 (en) | 2017-11-30 | 2022-04-06 | GeneMind Biosciences Company Limited | NUCLEOSIDE ANALOGUES, METHOD OF MANUFACTURE AND USE |
| US12215124B2 (en) | 2018-02-21 | 2025-02-04 | Singular Genomics Systems, Inc. | Fluorinated nucleotides and uses thereof |
| CN112218640A (zh) | 2018-03-15 | 2021-01-12 | 哥伦比亚大学董事会 | 核苷酸类似物及其在核酸测序和分析中的用途 |
| US20230011240A1 (en) | 2018-09-11 | 2023-01-12 | Singular Genomics Systems, Inc. | Modified archaeal family b polymerases |
| WO2020069424A1 (en) | 2018-09-28 | 2020-04-02 | Centrillion Technologies, Inc. | Disulfide-linked reversible terminators |
| CN113039191B (zh) * | 2018-10-25 | 2024-07-16 | 奇异基因组学系统公司 | 核苷酸类似物 |
| WO2020102980A1 (zh) * | 2018-11-20 | 2020-05-28 | 深圳华大生命科学研究院 | 测序用核苷酸的制备方法 |
| AU2019392932B2 (en) * | 2018-12-07 | 2023-11-02 | Element Biosciences, Inc. | Flow cell device and use thereof |
| US11293061B2 (en) | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
| JP2022516684A (ja) | 2019-01-08 | 2022-03-01 | シンギュラー・ゲノミクス・システムズ・インコーポレイテッド | ヌクレオチドの開裂可能なリンカーおよびその使用 |
| US11512295B2 (en) | 2019-09-12 | 2022-11-29 | Singular Genomics Systems, Inc. | Modified thermoccocus polymerases |
| WO2021067970A1 (en) * | 2019-10-04 | 2021-04-08 | Centrillion Technologies, Inc. | Reversible terminators for dna sequencing and methods of using the same |
| US11034942B1 (en) | 2020-02-27 | 2021-06-15 | Singular Genomics Systems, Inc. | Modified pyrococcus polymerases and uses thereof |
| US11174281B1 (en) | 2020-04-24 | 2021-11-16 | Singular Genomics Systems, Inc. | Modified nucleotides and uses thereof |
| WO2021226327A1 (en) * | 2020-05-08 | 2021-11-11 | Singular Genomics Systems, Inc. | Nucleotide cleavable linkers with rigid spacers and uses thereof |
| US11787831B2 (en) | 2020-06-22 | 2023-10-17 | Illumina Cambridge Limited | Nucleosides and nucleotides with 3′ acetal blocking group |
| WO2022094415A1 (en) | 2020-10-30 | 2022-05-05 | Singular Genomics Systems, Inc. | Methods and compositions for reducing nucleotide impurities |
| EP4036098B1 (en) * | 2021-02-02 | 2026-01-28 | Miltenyi Biotec B.V. & Co. KG | Synthesis of reversible nucleotide terminators by in-situ thio-alkyl group transfer for use in dna sequencing by synthesis |
| CA3214986A1 (en) * | 2021-04-01 | 2022-10-06 | Bgi Shenzhen | Nucleotide analogue for sequencing |
| EP4089098A1 (en) * | 2021-05-10 | 2022-11-16 | Miltenyi Biotec B.V. & Co. KG | Fret dye labeled reversible nucleotide terminators and their use in sequencing of dna |
| CN113341058A (zh) * | 2021-06-09 | 2021-09-03 | 南京溯远基因科技有限公司 | 一种三色高通量测序试剂及测序方法 |
| WO2023107673A2 (en) | 2021-12-10 | 2023-06-15 | Singular Genomics Systems, Inc. | Cleavable disulfide linkers and uses thereof |
| US20230242571A1 (en) * | 2022-02-02 | 2023-08-03 | Singular Genomics Systems, Inc. | Silicon containing modified nucleotide analogs |
| JP2025505253A (ja) * | 2022-02-11 | 2025-02-21 | ミルテニー バイオテック ベー．フェー． ウント コー． カー・ゲー | 酵素的ｄｎａおよびｒｎａ合成のための３’－オキシメチレンアルキルジスルフィド保護ヌクレオチドの使用 |
| EP4496799A2 (en) * | 2022-03-22 | 2025-01-29 | Dxome Co., Ltd. | Method of sequencing l-polynucleotide |
| WO2023192815A2 (en) * | 2022-03-28 | 2023-10-05 | Baylor College Of Medicine | Taxonomic signatures and methods of determining the same |
| CN115417907A (zh) * | 2022-08-15 | 2022-12-02 | 上海交通大学医学院 | 一种连接体及其制备方法 |
| EP4342998A1 (en) | 2022-09-23 | 2024-03-27 | Miltenyi Biotec B.V. & Co. KG | Labeled probes with differentially cleavable linkers and their use in de-coding dna and rna molecules |
| CN115960152A (zh) * | 2022-10-11 | 2023-04-14 | 河南师范大学 | 一种炔丙醚基取代tpt3型非天然碱基三磷酸及其合成方法和应用 |
| CN115785170A (zh) * | 2022-12-02 | 2023-03-14 | 重庆铭毅智造科技有限公司 | 一种可逆终止子核苷酸3’-oh保护基团的引入方法 |
| WO2025072834A2 (en) * | 2023-09-27 | 2025-04-03 | Dxome Co., Ltd. | Method of sequencing and synthesizing l-polynucleotide |
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| NO873956L (no) | 1986-09-25 | 1988-03-28 | Bp Chem Int Ltd | Rensemidler inneholdende peroksyder for fremstilling av flyktige sulfider. |
| US5756334A (en) | 1990-04-26 | 1998-05-26 | New England Biolabs, Inc. | Thermostable DNA polymerase from 9°N-7 and methods for producing the same |
| US5633360A (en) * | 1992-04-14 | 1997-05-27 | Gilead Sciences, Inc. | Oligonucleotide analogs capable of passive cell membrane permeation |
| US5990300A (en) | 1994-09-02 | 1999-11-23 | Andrew C. Hiatt | Enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
| US6046039A (en) | 1998-08-19 | 2000-04-04 | Battelle Memorial Institute | Methods for producing partially digested restriction DNA fragments and for producing a partially modified PCR product |
| US6465628B1 (en) | 1999-02-04 | 2002-10-15 | Isis Pharmaceuticals, Inc. | Process for the synthesis of oligomeric compounds |
| AU2002301870A1 (en) * | 1999-03-12 | 2003-03-13 | President And Fellows Of Harvard College | Nuclotide Compounds Having a Cleavable Linker |
| AU7537200A (en) | 1999-09-29 | 2001-04-30 | Solexa Ltd. | Polynucleotide sequencing |
| US6309836B1 (en) * | 1999-10-05 | 2001-10-30 | Marek Kwiatkowski | Compounds for protecting hydroxyls and methods for their use |
| US6498189B1 (en) | 1999-11-10 | 2002-12-24 | University Of New Mexico | Method for induction of L-selectin shedding |
| AU2001296645A1 (en) | 2000-10-06 | 2002-04-15 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding dna and rna |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| GB0129012D0 (en) * | 2001-12-04 | 2002-01-23 | Solexa Ltd | Labelled nucleotides |
| US7414116B2 (en) | 2002-08-23 | 2008-08-19 | Illumina Cambridge Limited | Labelled nucleotides |
| US20040235027A1 (en) * | 2003-03-31 | 2004-11-25 | Regent Of The University Of California | Preparation and application of ligand-biopolymer conjugates |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US20070117104A1 (en) | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
| GB2446083B (en) | 2005-10-31 | 2011-03-02 | Univ Columbia | Chemically cleavable 3'-0-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods |
| WO2008042067A2 (en) | 2006-09-28 | 2008-04-10 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| GB2457402B (en) | 2006-12-01 | 2011-10-19 | Univ Columbia | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| US8481259B2 (en) * | 2007-02-05 | 2013-07-09 | Intelligent Bio-Systems, Inc. | Methods and devices for sequencing nucleic acids in smaller batches |
| DK2725107T3 (da) * | 2007-10-19 | 2019-01-02 | Univ Columbia | DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer |
| US8017338B2 (en) | 2007-11-20 | 2011-09-13 | Life Technologies Corporation | Reversible di-nucleotide terminator sequencing |
| US8101353B2 (en) | 2007-12-18 | 2012-01-24 | Advanced Analytical Technologies, Inc. | System and method for nucleotide sequence profiling for sample identification |
| US8623598B2 (en) | 2008-03-19 | 2014-01-07 | Intelligent Bio Systems, Inc. | Methods and compositions for inhibiting undesired cleaving of labels |
| US9017973B2 (en) | 2008-03-19 | 2015-04-28 | Intelligent Biosystems, Inc. | Methods and compositions for incorporating nucleotides |
| US20100330569A1 (en) | 2009-04-23 | 2010-12-30 | Intelligent Bio-Systems, Inc. | Hydroxymethyl Linkers For Labeling Nucleotides |
| WO2013040257A1 (en) * | 2011-09-13 | 2013-03-21 | Lasergen, Inc. | 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing |
| CN103484106B (zh) * | 2013-09-05 | 2015-08-19 | 上海交通大学 | 四色荧光标记可逆终端及其在dna测序中的用途 |
| EP3066105A4 (en) * | 2013-11-06 | 2017-10-11 | Solstice Biologics, Ltd. | Polynucleotide constructs having disulfide groups |
| EP2876166B1 (en) * | 2013-11-20 | 2016-12-14 | Roche Diagnostics GmbH | New compound for sequencing by synthesis |
| CN108779138B (zh) * | 2015-09-28 | 2022-06-17 | 哥伦比亚大学董事会 | 用作dna合成测序的可逆终止物的基于新的二硫键接头的核苷酸的设计与合成 |
| TWI639614B (zh) * | 2015-11-06 | 2018-11-01 | 美商齊亞雋科學公司 | 核苷酸類似物 |
-
2016
- 2016-11-04 TW TW105135999A patent/TWI639614B/zh not_active IP Right Cessation
- 2016-11-04 CA CA3158679A patent/CA3158679A1/en active Pending
- 2016-11-04 EP EP16863015.0A patent/EP3370731A4/en not_active Withdrawn
- 2016-11-04 US US15/343,279 patent/US10273539B2/en active Active
- 2016-11-04 JP JP2018522968A patent/JP6775014B2/ja not_active Expired - Fee Related
- 2016-11-04 AU AU2016349457A patent/AU2016349457B2/en active Active
- 2016-11-04 KR KR1020187015618A patent/KR102298737B1/ko not_active Expired - Fee Related
- 2016-11-04 TW TW107128049A patent/TWI664160B/zh active
- 2016-11-04 KR KR1020217027925A patent/KR102454782B1/ko active Active
- 2016-11-04 US US15/343,341 patent/US10336785B2/en active Active
- 2016-11-04 CA CA3004060A patent/CA3004060C/en active Active
- 2016-11-04 CN CN201680074649.5A patent/CN109476694B/zh active Active
- 2016-11-04 WO PCT/US2016/060435 patent/WO2017079498A2/en not_active Ceased
- 2016-11-04 US US15/343,267 patent/US10301346B2/en active Active
- 2016-11-04 TW TW108112168A patent/TWI698530B/zh not_active IP Right Cessation
-
2018
- 2018-03-22 US US15/928,562 patent/US11548909B2/en active Active
- 2018-03-22 US US15/928,533 patent/US11046726B2/en active Active
-
2019
- 2019-05-28 US US16/423,466 patent/US11117921B2/en not_active Expired - Fee Related
-
2020
- 2020-10-05 JP JP2020168502A patent/JP7119041B2/ja not_active Expired - Fee Related
-
2021
- 2021-06-28 US US17/360,427 patent/US20220162251A1/en not_active Abandoned
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