JP2020534812A5 - - Google Patents
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- JP2020534812A5 JP2020534812A5 JP2020513724A JP2020513724A JP2020534812A5 JP 2020534812 A5 JP2020534812 A5 JP 2020534812A5 JP 2020513724 A JP2020513724 A JP 2020513724A JP 2020513724 A JP2020513724 A JP 2020513724A JP 2020534812 A5 JP2020534812 A5 JP 2020534812A5
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- JP
- Japan
- Prior art keywords
- nucleic acid
- acid molecule
- protein
- donor
- regulatory
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 68
- 102000039446 nucleic acids Human genes 0.000 claims 66
- 108020004707 nucleic acids Proteins 0.000 claims 66
- 150000007523 nucleic acids Chemical class 0.000 claims 66
- 210000004027 cell Anatomy 0.000 claims 25
- 239000003623 enhancer Substances 0.000 claims 20
- 108091033409 CRISPR Proteins 0.000 claims 19
- 230000004568 DNA-binding Effects 0.000 claims 19
- 230000001105 regulatory effect Effects 0.000 claims 17
- 101710163270 Nuclease Proteins 0.000 claims 15
- 239000003550 marker Substances 0.000 claims 15
- 108090000623 proteins and genes Proteins 0.000 claims 15
- 102000004169 proteins and genes Human genes 0.000 claims 14
- 108020004414 DNA Proteins 0.000 claims 13
- 238000003776 cleavage reaction Methods 0.000 claims 13
- 230000007017 scission Effects 0.000 claims 13
- 102000053602 DNA Human genes 0.000 claims 12
- 150000001413 amino acids Chemical class 0.000 claims 12
- 230000027455 binding Effects 0.000 claims 12
- 230000003834 intracellular effect Effects 0.000 claims 11
- 108700008625 Reporter Genes Proteins 0.000 claims 9
- 208000009869 Neu-Laxova syndrome Diseases 0.000 claims 8
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 8
- 230000006780 non-homologous end joining Effects 0.000 claims 8
- 238000010354 CRISPR gene editing Methods 0.000 claims 6
- 230000000694 effects Effects 0.000 claims 6
- 108020001507 fusion proteins Proteins 0.000 claims 6
- 102000037865 fusion proteins Human genes 0.000 claims 6
- 108091006104 gene-regulatory proteins Proteins 0.000 claims 6
- 102000034356 gene-regulatory proteins Human genes 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 6
- 125000003729 nucleotide group Chemical group 0.000 claims 6
- 108020005004 Guide RNA Proteins 0.000 claims 5
- BCCRXDTUTZHDEU-VKHMYHEASA-N Gly-Ser Chemical compound NCC(=O)N[C@@H](CO)C(O)=O BCCRXDTUTZHDEU-VKHMYHEASA-N 0.000 claims 4
- 230000005782 double-strand break Effects 0.000 claims 4
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 claims 4
- 239000003112 inhibitor Substances 0.000 claims 4
- 238000002372 labelling Methods 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 claims 3
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical group OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 claims 2
- 101710185494 Zinc finger protein Proteins 0.000 claims 2
- 102100023597 Zinc finger protein 816 Human genes 0.000 claims 2
- 125000003277 amino group Chemical group 0.000 claims 2
- 210000004899 c-terminal region Anatomy 0.000 claims 2
- 239000012636 effector Substances 0.000 claims 2
- 230000006801 homologous recombination Effects 0.000 claims 2
- 238000002744 homologous recombination Methods 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 230000002103 transcriptional effect Effects 0.000 claims 2
- AQPVUEJJARLJHB-BQBZGAKWSA-N Arg-Gly-Ala Chemical compound OC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](N)CCCN=C(N)N AQPVUEJJARLJHB-BQBZGAKWSA-N 0.000 claims 1
- 108020004705 Codon Proteins 0.000 claims 1
- 102000005768 DNA-Activated Protein Kinase Human genes 0.000 claims 1
- 108010006124 DNA-Activated Protein Kinase Proteins 0.000 claims 1
- CVRUVYDNRPSKBM-QEJZJMRPSA-N Gln-Trp-Ser Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CCC(=O)N)N CVRUVYDNRPSKBM-QEJZJMRPSA-N 0.000 claims 1
- 102000018697 Membrane Proteins Human genes 0.000 claims 1
- 108010052285 Membrane Proteins Proteins 0.000 claims 1
- 102000004389 Ribonucleoproteins Human genes 0.000 claims 1
- 108010081734 Ribonucleoproteins Proteins 0.000 claims 1
- 241000700584 Simplexvirus Species 0.000 claims 1
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 1
- 108091081024 Start codon Proteins 0.000 claims 1
- 102000006601 Thymidine Kinase Human genes 0.000 claims 1
- 108020004440 Thymidine kinase Proteins 0.000 claims 1
- 108010073062 Transcription Activator-Like Effectors Proteins 0.000 claims 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 1
- 239000007801 affinity label Substances 0.000 claims 1
- -1 amino acid amino acids Chemical class 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940045988 antineoplastic drug protein kinase inhibitors Drugs 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 claims 1
- 239000002458 cell surface marker Substances 0.000 claims 1
- 210000003763 chloroplast Anatomy 0.000 claims 1
- 239000013604 expression vector Substances 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- 210000003470 mitochondria Anatomy 0.000 claims 1
- 210000004940 nucleus Anatomy 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 239000003909 protein kinase inhibitor Substances 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
- 239000013598 vector Substances 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023145587A JP2023168355A (ja) | 2017-09-08 | 2023-09-07 | 改良された相同組換えおよびその組成物のための方法 |
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762555862P | 2017-09-08 | 2017-09-08 | |
| US62/555,862 | 2017-09-08 | ||
| US201762568661P | 2017-10-05 | 2017-10-05 | |
| US62/568,661 | 2017-10-05 | ||
| US201762574936P | 2017-10-20 | 2017-10-20 | |
| US62/574,936 | 2017-10-20 | ||
| US201862626792P | 2018-02-06 | 2018-02-06 | |
| US62/626,792 | 2018-02-06 | ||
| US201862717403P | 2018-08-10 | 2018-08-10 | |
| US62/717,403 | 2018-08-10 | ||
| PCT/US2018/049966 WO2019051237A1 (en) | 2017-09-08 | 2018-09-07 | ENHANCED RECOMBINANT RECOMBINATION METHODS AND COMPOSITIONS THEREOF |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023145587A Division JP2023168355A (ja) | 2017-09-08 | 2023-09-07 | 改良された相同組換えおよびその組成物のための方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020534812A JP2020534812A (ja) | 2020-12-03 |
| JP2020534812A5 true JP2020534812A5 (https=) | 2021-10-14 |
Family
ID=63714051
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020513724A Pending JP2020534812A (ja) | 2017-09-08 | 2018-09-07 | 改良された相同組換えおよびその組成物のための方法 |
| JP2023145587A Pending JP2023168355A (ja) | 2017-09-08 | 2023-09-07 | 改良された相同組換えおよびその組成物のための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023145587A Pending JP2023168355A (ja) | 2017-09-08 | 2023-09-07 | 改良された相同組換えおよびその組成物のための方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20190119701A1 (https=) |
| EP (1) | EP3679143A1 (https=) |
| JP (2) | JP2020534812A (https=) |
| CN (2) | CN119570864A (https=) |
| WO (1) | WO2019051237A1 (https=) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| WO2015105955A1 (en) | 2014-01-08 | 2015-07-16 | Flodesign Sonics, Inc. | Acoustophoresis device with dual acoustophoretic chamber |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| CN109477130B (zh) | 2016-07-01 | 2022-08-30 | 微软技术许可有限责任公司 | 通过迭代dna编辑的存储 |
| US11359234B2 (en) | 2016-07-01 | 2022-06-14 | Microsoft Technology Licensing, Llc | Barcoding sequences for identification of gene expression |
| US20180004537A1 (en) | 2016-07-01 | 2018-01-04 | Microsoft Technology Licensing, Llc | Molecular State Machines |
| WO2019118921A1 (en) | 2017-12-14 | 2019-06-20 | Flodesign Sonics, Inc. | Acoustic transducer drive and controller |
| WO2019204226A1 (en) * | 2018-04-16 | 2019-10-24 | University Of Massachusetts | Compositions and methods for improved gene editing |
| WO2020072699A1 (en) * | 2018-10-02 | 2020-04-09 | Exosome Therapeutics, Inc. | Exosome loaded therapeutics for treating sickle cell disease |
| US12312584B2 (en) * | 2018-10-02 | 2025-05-27 | Exosome Therapeutics, Inc. | cGMP exosome loaded therapeutics for treating sickle cell disease |
| EP3870604B1 (en) | 2018-10-26 | 2022-11-23 | F. Hoffmann-La Roche AG | Multispecific antibody screening method using recombinase mediated cassette exchange |
| CN111304248B (zh) * | 2018-12-25 | 2021-08-24 | 百奥赛图江苏基因生物技术有限公司 | 人源化细胞因子il15基因改造非人动物的构建方法及应用 |
| CN113661173B (zh) | 2019-03-29 | 2024-10-01 | 豪夫迈·罗氏有限公司 | 通过以限定的组织形式靶向整合多个表达盒来产生表达FcRn的细胞的方法 |
| WO2020206162A1 (en) * | 2019-04-03 | 2020-10-08 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for insertion of antibody coding sequences into a safe harbor locus |
| US20230193322A1 (en) * | 2019-04-16 | 2023-06-22 | Arizona Board Of Regents On Behalf Of Arizona State University | CAS9 Fusion Proteins and Related Methods |
| WO2020257684A1 (en) * | 2019-06-20 | 2020-12-24 | University Of Massachusetts | Compositions and methods for improved gene editing |
| JP7579817B2 (ja) | 2019-07-03 | 2024-11-08 | インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド | Ｃｒｉｓｐｒで導入された二本鎖ｄｎａ切断修復の同定、特徴付けおよび定量化 |
| US20210079387A1 (en) * | 2019-08-27 | 2021-03-18 | The Jackson Laboratory | Cleavage-resistant donor nucleic acids and methods of use |
| US20220275400A1 (en) * | 2019-08-30 | 2022-09-01 | The Trustees Of Columbia University In The City Of New York | Methods for scalable gene insertions |
| JP7762651B2 (ja) * | 2019-10-24 | 2025-10-30 | インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド | 修飾二重鎖ドナー鋳型 |
| US20230086199A1 (en) * | 2019-11-26 | 2023-03-23 | The Broad Institute, Inc. | Systems and methods for evaluating cas9-independent off-target editing of nucleic acids |
| JP2024513087A (ja) * | 2021-04-07 | 2024-03-21 | アストラゼネカ・アクチエボラーグ | 部位特異的改変のための組成物及び方法 |
| EP4083227A1 (en) * | 2021-04-29 | 2022-11-02 | 4basebio, S.L.U. | Linear dna with enhanced resistance against exonucleases |
| JP2024533311A (ja) * | 2021-09-08 | 2024-09-12 | フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー | ゲノムを調節するための方法及び組成物 |
| CN115029277B (zh) * | 2022-06-24 | 2023-08-22 | 四川省烟草公司凉山州公司 | 一株耐酸抗青枯病的假蕈状芽孢杆菌 |
| WO2025171070A1 (en) * | 2024-02-06 | 2025-08-14 | Integrated Dna Technologies, Inc. | Optimized spcas9 proteins for efficient genome editing in eukaryotic cells |
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| US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5334575A (en) | 1992-12-17 | 1994-08-02 | Eastman Kodak Company | Dye-containing beads for laser-induced thermal dye transfer |
| US5830645A (en) | 1994-12-09 | 1998-11-03 | The Regents Of The University Of California | Comparative fluorescence hybridization to nucleic acid arrays |
| US6051429A (en) * | 1995-06-07 | 2000-04-18 | Life Technologies, Inc. | Peptide-enhanced cationic lipid transfections |
| US5888795A (en) | 1997-09-09 | 1999-03-30 | Becton, Dickinson And Company | Thermostable uracil DNA glycosylase and methods of use |
| US7592434B2 (en) | 2003-04-04 | 2009-09-22 | Verenium Corporation | Pectate lyases, nucleic encoding them and methods for making and using them |
| WO2008151219A1 (en) | 2007-06-04 | 2008-12-11 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| US20120329067A1 (en) | 2010-01-22 | 2012-12-27 | Barbas Iii Carlos F | Methods of Generating Zinc Finger Nucleases Having Altered Activity |
| ES2388020B1 (es) * | 2011-03-10 | 2013-10-01 | Universitat De Girona | Proteínas recombinantes con efecto antitumoral. |
| WO2012168307A2 (en) * | 2011-06-07 | 2012-12-13 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Improved recombination efficiency by inhibition of nhej dna repair |
| WO2013152220A2 (en) | 2012-04-04 | 2013-10-10 | Life Technologies Corporation | Tal-effector assembly platform, customized services, kits and assays |
| AU2013359262C1 (en) * | 2012-12-12 | 2021-05-13 | Massachusetts Institute Of Technology | CRISPR-Cas component systems, methods and compositions for sequence manipulation |
| US10227610B2 (en) * | 2013-02-25 | 2019-03-12 | Sangamo Therapeutics, Inc. | Methods and compositions for enhancing nuclease-mediated gene disruption |
| MX374090B (es) * | 2013-03-14 | 2025-03-05 | Caribou Biosciences Inc | Composiciones y métodos de ácidos nucleicos dirigidos al ácido nucleico. |
| SI2986729T1 (sl) * | 2013-04-16 | 2019-02-28 | Regeneron Pharmaceuticals, Inc. | Ciljana sprememba genoma podgane |
| US9546384B2 (en) * | 2013-12-11 | 2017-01-17 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for the targeted modification of a mouse genome |
| EP3470089A1 (en) * | 2013-12-12 | 2019-04-17 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components |
| MX2016007797A (es) * | 2013-12-19 | 2016-09-07 | Amyris Inc | Metodos para integracion genomica. |
| RS60359B1 (sr) * | 2014-06-06 | 2020-07-31 | Regeneron Pharma | Postupci i kompozicije za modifikovanje ciljanog lokusa |
| EP4710943A3 (en) * | 2015-08-28 | 2026-04-29 | Molecular Transfer, Inc. | Transfection complexes and methods of using the same |
| JP2018532404A (ja) * | 2015-10-14 | 2018-11-08 | ライフ テクノロジーズ コーポレーション | リボ核タンパク質トランスフェクション薬剤 |
| CN108779074A (zh) * | 2016-03-01 | 2018-11-09 | 分子传递有限公司 | 植物病毒移动蛋白和其使用方法 |
| WO2018013932A1 (en) * | 2016-07-15 | 2018-01-18 | Salk Institute For Biological Studies | Methods and compositions for genome editing in non-dividing cells |
| US20200299729A1 (en) * | 2017-09-08 | 2020-09-24 | Life Technologies Corporation | Methods for improved homologous recombination and compositions thereof |
-
2018
- 2018-09-07 EP EP18779883.0A patent/EP3679143A1/en active Pending
- 2018-09-07 US US16/124,931 patent/US20190119701A1/en not_active Abandoned
- 2018-09-07 WO PCT/US2018/049966 patent/WO2019051237A1/en not_active Ceased
- 2018-09-07 CN CN202411245009.9A patent/CN119570864A/zh active Pending
- 2018-09-07 JP JP2020513724A patent/JP2020534812A/ja active Pending
- 2018-09-07 CN CN201880071907.3A patent/CN111315889A/zh active Pending
-
2023
- 2023-09-07 JP JP2023145587A patent/JP2023168355A/ja active Pending
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