JP2020534812A - 改良された相同組換えおよびその組成物のための方法 - Google Patents

改良された相同組換えおよびその組成物のための方法 Download PDF

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JP2020534812A
JP2020534812A JP2020513724A JP2020513724A JP2020534812A JP 2020534812 A JP2020534812 A JP 2020534812A JP 2020513724 A JP2020513724 A JP 2020513724A JP 2020513724 A JP2020513724 A JP 2020513724A JP 2020534812 A JP2020534812 A JP 2020534812A
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nucleic acid
protein
dna
regulatory
acid molecule
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シークアン リャン
シークアン リャン
ロバート ポッター
ロバート ポッター
ランシャ ポン
ランシャ ポン
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ライフ テクノロジーズ コーポレイション
ライフ テクノロジーズ コーポレイション
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JP2020513724A 2017-09-08 2018-09-07 改良された相同組換えおよびその組成物のための方法 Pending JP2020534812A (ja)

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US201762555862P 2017-09-08 2017-09-08
US62/555,862 2017-09-08
US201762568661P 2017-10-05 2017-10-05
US62/568,661 2017-10-05
US201762574936P 2017-10-20 2017-10-20
US62/574,936 2017-10-20
US201862626792P 2018-02-06 2018-02-06
US62/626,792 2018-02-06
US201862717403P 2018-08-10 2018-08-10
US62/717,403 2018-08-10
PCT/US2018/049966 WO2019051237A1 (en) 2017-09-08 2018-09-07 ENHANCED RECOMBINANT RECOMBINATION METHODS AND COMPOSITIONS THEREOF

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Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
ES2817973T3 (es) 2016-07-01 2021-04-08 Microsoft Technology Licensing Llc Almacenamiento a través de la edición iterativa del ADN
US20180004537A1 (en) 2016-07-01 2018-01-04 Microsoft Technology Licensing, Llc Molecular State Machines
US11359234B2 (en) 2016-07-01 2022-06-14 Microsoft Technology Licensing, Llc Barcoding sequences for identification of gene expression
EP3679143A1 (en) 2017-09-08 2020-07-15 Life Technologies Corporation Methods for improved homologous recombination and compositions thereof
KR102439221B1 (ko) 2017-12-14 2022-09-01 프로디자인 소닉스, 인크. 음향 트랜스듀서 구동기 및 제어기
EP3781677A4 (en) * 2018-04-16 2022-01-19 University of Massachusetts COMPOSITIONS AND METHODS FOR IMPROVED GENE EDITTING
US12312584B2 (en) * 2018-10-02 2025-05-27 Exosome Therapeutics, Inc. cGMP exosome loaded therapeutics for treating sickle cell disease
US20200102563A1 (en) * 2018-10-02 2020-04-02 Exosome Therapeutics, Inc. Exosome loaded therapeutics for treating sickle cell disease
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
CN111304248B (zh) * 2018-12-25 2021-08-24 百奥赛图江苏基因生物技术有限公司 人源化细胞因子il15基因改造非人动物的构建方法及应用
WO2020159470A1 (en) * 2019-01-28 2020-08-06 Mayo Foundation For Medical Education And Research Mitochondrial genome editing methods
JP7250950B2 (ja) 2019-03-29 2023-04-03 エフ. ホフマン-ラ ロシュ アーゲー 所定の構成の複数の発現カセットを標的指向組込みすることによってFcRn発現細胞を作製するための方法
AU2020256225B9 (en) * 2019-04-03 2025-04-10 Regeneron Pharmaceuticals, Inc. Methods and compositions for insertion of antibody coding sequences into a safe harbor locus
WO2020214610A1 (en) * 2019-04-16 2020-10-22 Arizona Board Of Regents On Behalf Of Arizona State University Cas9 fusion proteins and related methods
US20220348912A1 (en) * 2019-06-20 2022-11-03 University Of Massachusetts Compositions and methods for improved gene editing
CA3144106A1 (en) 2019-07-03 2021-01-07 Integrated Dna Technologies, Inc. Identification, characterization, and quantitation of crispr-introduced double-stranded dna break repairs
US20210079387A1 (en) * 2019-08-27 2021-03-18 The Jackson Laboratory Cleavage-resistant donor nucleic acids and methods of use
US20220275400A1 (en) * 2019-08-30 2022-09-01 The Trustees Of Columbia University In The City Of New York Methods for scalable gene insertions
CN119193713A (zh) * 2019-10-24 2024-12-27 合成Dna技术公司 修饰的双链供体模板
US20230086199A1 (en) * 2019-11-26 2023-03-23 The Broad Institute, Inc. Systems and methods for evaluating cas9-independent off-target editing of nucleic acids
WO2022214522A2 (en) * 2021-04-07 2022-10-13 Astrazeneca Ab Compositions and methods for site-specific modification
EP4083227A1 (en) * 2021-04-29 2022-11-02 4basebio, S.L.U. Linear dna with enhanced resistance against exonucleases
AU2022343268A1 (en) 2021-09-08 2024-03-28 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
CN115029277B (zh) * 2022-06-24 2023-08-22 四川省烟草公司凉山州公司 一株耐酸抗青枯病的假蕈状芽孢杆菌
US20260103695A1 (en) * 2024-02-06 2026-04-16 Integrated Dna Technologies, Inc. Optimized SPCAS9 Proteins for Efficient Genome Editing in Eukaryotic Cells

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001517939A (ja) * 1997-03-14 2001-10-09 ライフ テクノロジーズ,インコーポレイテッド ペプチドによって増強されるトランスフェクション
US20140315985A1 (en) * 2013-03-14 2014-10-23 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
JP2016509063A (ja) * 2013-02-25 2016-03-24 サンガモ バイオサイエンシーズ, インコーポレイテッド ヌクレアーゼ媒介性遺伝子破壊を増強するための方法および組成物
JP2016515401A (ja) * 2013-04-16 2016-05-30 リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. ラットゲノムの標的改変
JP2016539655A (ja) * 2013-12-11 2016-12-22 リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. ゲノムの標的改変のための方法及び組成物
WO2017066707A1 (en) * 2015-10-14 2017-04-20 Life Technologies Corporation Ribonucleoprotein transfection agents

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5334575A (en) 1992-12-17 1994-08-02 Eastman Kodak Company Dye-containing beads for laser-induced thermal dye transfer
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US5888795A (en) 1997-09-09 1999-03-30 Becton, Dickinson And Company Thermostable uracil DNA glycosylase and methods of use
WO2003012036A2 (en) 2001-07-27 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
HUE030493T2 (en) 2003-04-04 2017-05-29 Basf Enzymes Llc Pectate lyases, nucleic acids and processes encoding them for their production and use
EP2150617B1 (en) 2007-06-04 2014-10-22 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
CA2720903C (en) 2008-04-14 2019-01-15 Sangamo Biosciences, Inc. Linear donor constructs for targeted integration
US20120329067A1 (en) 2010-01-22 2012-12-27 Barbas Iii Carlos F Methods of Generating Zinc Finger Nucleases Having Altered Activity
EP2660318A1 (en) 2010-02-09 2013-11-06 Sangamo BioSciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
ES2388020B1 (es) * 2011-03-10 2013-10-01 Universitat De Girona Proteínas recombinantes con efecto antitumoral.
WO2012168307A2 (en) * 2011-06-07 2012-12-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Improved recombination efficiency by inhibition of nhej dna repair
WO2013152220A2 (en) 2012-04-04 2013-10-10 Life Technologies Corporation Tal-effector assembly platform, customized services, kits and assays
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
EP2840140B2 (en) * 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
DK2931891T3 (da) 2012-12-17 2019-08-19 Harvard College Rna-styret modificering af menneskelige genomer
WO2014102688A1 (en) 2012-12-27 2014-07-03 Cellectis New design matrix for improvement of homology-directed gene targeting
US9902973B2 (en) 2013-04-11 2018-02-27 Caribou Biosciences, Inc. Methods of modifying a target nucleic acid with an argonaute
EP2796558A1 (en) 2013-04-23 2014-10-29 Rheinische Friedrich-Wilhelms-Universität Bonn Improved gene targeting and nucleic acid carrier molecule, in particular for use in plants
CA2930877A1 (en) 2013-11-18 2015-05-21 Crispr Therapeutics Ag Crispr-cas system materials and methods
US10787684B2 (en) * 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
EP3470089A1 (en) * 2013-12-12 2019-04-17 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
EP3083958B1 (en) * 2013-12-19 2019-04-17 Amyris, Inc. Methods for genomic integration
HRP20200529T1 (hr) * 2014-06-06 2020-09-04 Regeneron Pharmaceuticals, Inc. Postupci i pripravci za modificiranje ciljnog lokusa
SG11201701245QA (en) * 2014-08-27 2017-03-30 Caribou Biosciences Inc Methods for increasing cas9-mediated engineering efficiency
EP3201340B1 (en) 2014-10-01 2020-12-02 The General Hospital Corporation Methods for increasing efficiency of nuclease-induced homology-directed repair
US9879283B2 (en) 2014-10-09 2018-01-30 Life Technologies Corporation CRISPR oligonucleotides and gene editing
WO2016065364A1 (en) 2014-10-24 2016-04-28 Life Technologies Corporation Compositions and methods for enhancing homologous recombination
EP4464338A3 (en) 2014-11-07 2025-02-12 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
US10190106B2 (en) * 2014-12-22 2019-01-29 Univesity Of Massachusetts Cas9-DNA targeting unit chimeras
CN118271422A (zh) * 2015-08-28 2024-07-02 分子传递有限公司 转染络合物和其使用方法
WO2017053729A1 (en) 2015-09-25 2017-03-30 The Board Of Trustees Of The Leland Stanford Junior University Nuclease-mediated genome editing of primary cells and enrichment thereof
CA2947904A1 (en) 2015-11-12 2017-05-12 Pfizer Inc. Tissue-specific genome engineering using crispr-cas9
WO2017096041A1 (en) 2015-12-02 2017-06-08 The Regents Of The University Of California Compositions and methods for modifying a target nucleic acid
EP3423438A4 (en) * 2016-03-01 2020-05-06 Molecular Transfer, Inc. PLANT VIRUS MOTOR PROTEINS AND METHOD FOR USE THEREOF
US10428327B2 (en) 2016-05-27 2019-10-01 Life Technologies Corporation Compositions and methods for enhancing homologous recombination
EP4321623A3 (en) * 2016-07-15 2024-05-15 Salk Institute for Biological Studies Methods and compositions for genome editing in non-dividing cells
EP3679144A1 (en) * 2017-09-08 2020-07-15 Life Technologies Corporation Methods for improved homologous recombination and compositions thereof
EP3679143A1 (en) 2017-09-08 2020-07-15 Life Technologies Corporation Methods for improved homologous recombination and compositions thereof
EP3688159A4 (en) * 2017-09-26 2021-10-20 The Board of Trustees of the University of Illinois CRISPR / CAS SYSTEM AND PROCESS FOR GENOME EDITING AND TRANSCRIPT MODULATION

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001517939A (ja) * 1997-03-14 2001-10-09 ライフ テクノロジーズ,インコーポレイテッド ペプチドによって増強されるトランスフェクション
JP2016509063A (ja) * 2013-02-25 2016-03-24 サンガモ バイオサイエンシーズ, インコーポレイテッド ヌクレアーゼ媒介性遺伝子破壊を増強するための方法および組成物
US20140315985A1 (en) * 2013-03-14 2014-10-23 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
JP2016515401A (ja) * 2013-04-16 2016-05-30 リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. ラットゲノムの標的改変
JP2016539655A (ja) * 2013-12-11 2016-12-22 リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. ゲノムの標的改変のための方法及び組成物
WO2017066707A1 (en) * 2015-10-14 2017-04-20 Life Technologies Corporation Ribonucleoprotein transfection agents

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