JP2020534812A - 改良された相同組換えおよびその組成物のための方法 - Google Patents
改良された相同組換えおよびその組成物のための方法 Download PDFInfo
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- JP2020534812A JP2020534812A JP2020513724A JP2020513724A JP2020534812A JP 2020534812 A JP2020534812 A JP 2020534812A JP 2020513724 A JP2020513724 A JP 2020513724A JP 2020513724 A JP2020513724 A JP 2020513724A JP 2020534812 A JP2020534812 A JP 2020534812A
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2800/00—Nucleic acids vectors
- C12N2800/95—Protection of vectors from inactivation by agents such as antibodies or enzymes, e.g. using polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
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- Health & Medical Sciences (AREA)
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- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023145587A JP2023168355A (ja) | 2017-09-08 | 2023-09-07 | 改良された相同組換えおよびその組成物のための方法 |
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762555862P | 2017-09-08 | 2017-09-08 | |
| US62/555,862 | 2017-09-08 | ||
| US201762568661P | 2017-10-05 | 2017-10-05 | |
| US62/568,661 | 2017-10-05 | ||
| US201762574936P | 2017-10-20 | 2017-10-20 | |
| US62/574,936 | 2017-10-20 | ||
| US201862626792P | 2018-02-06 | 2018-02-06 | |
| US62/626,792 | 2018-02-06 | ||
| US201862717403P | 2018-08-10 | 2018-08-10 | |
| US62/717,403 | 2018-08-10 | ||
| PCT/US2018/049966 WO2019051237A1 (en) | 2017-09-08 | 2018-09-07 | ENHANCED RECOMBINANT RECOMBINATION METHODS AND COMPOSITIONS THEREOF |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023145587A Division JP2023168355A (ja) | 2017-09-08 | 2023-09-07 | 改良された相同組換えおよびその組成物のための方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020534812A true JP2020534812A (ja) | 2020-12-03 |
| JP2020534812A5 JP2020534812A5 (https=) | 2021-10-14 |
Family
ID=63714051
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020513724A Pending JP2020534812A (ja) | 2017-09-08 | 2018-09-07 | 改良された相同組換えおよびその組成物のための方法 |
| JP2023145587A Pending JP2023168355A (ja) | 2017-09-08 | 2023-09-07 | 改良された相同組換えおよびその組成物のための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023145587A Pending JP2023168355A (ja) | 2017-09-08 | 2023-09-07 | 改良された相同組換えおよびその組成物のための方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20190119701A1 (https=) |
| EP (1) | EP3679143A1 (https=) |
| JP (2) | JP2020534812A (https=) |
| CN (2) | CN119570864A (https=) |
| WO (1) | WO2019051237A1 (https=) |
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| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| WO2015105955A1 (en) | 2014-01-08 | 2015-07-16 | Flodesign Sonics, Inc. | Acoustophoresis device with dual acoustophoretic chamber |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| CN109477130B (zh) | 2016-07-01 | 2022-08-30 | 微软技术许可有限责任公司 | 通过迭代dna编辑的存储 |
| US11359234B2 (en) | 2016-07-01 | 2022-06-14 | Microsoft Technology Licensing, Llc | Barcoding sequences for identification of gene expression |
| US20180004537A1 (en) | 2016-07-01 | 2018-01-04 | Microsoft Technology Licensing, Llc | Molecular State Machines |
| WO2019118921A1 (en) | 2017-12-14 | 2019-06-20 | Flodesign Sonics, Inc. | Acoustic transducer drive and controller |
| WO2019204226A1 (en) * | 2018-04-16 | 2019-10-24 | University Of Massachusetts | Compositions and methods for improved gene editing |
| WO2020072699A1 (en) * | 2018-10-02 | 2020-04-09 | Exosome Therapeutics, Inc. | Exosome loaded therapeutics for treating sickle cell disease |
| US12312584B2 (en) * | 2018-10-02 | 2025-05-27 | Exosome Therapeutics, Inc. | cGMP exosome loaded therapeutics for treating sickle cell disease |
| EP3870604B1 (en) | 2018-10-26 | 2022-11-23 | F. Hoffmann-La Roche AG | Multispecific antibody screening method using recombinase mediated cassette exchange |
| CN111304248B (zh) * | 2018-12-25 | 2021-08-24 | 百奥赛图江苏基因生物技术有限公司 | 人源化细胞因子il15基因改造非人动物的构建方法及应用 |
| CN113661173B (zh) | 2019-03-29 | 2024-10-01 | 豪夫迈·罗氏有限公司 | 通过以限定的组织形式靶向整合多个表达盒来产生表达FcRn的细胞的方法 |
| WO2020206162A1 (en) * | 2019-04-03 | 2020-10-08 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for insertion of antibody coding sequences into a safe harbor locus |
| US20230193322A1 (en) * | 2019-04-16 | 2023-06-22 | Arizona Board Of Regents On Behalf Of Arizona State University | CAS9 Fusion Proteins and Related Methods |
| WO2020257684A1 (en) * | 2019-06-20 | 2020-12-24 | University Of Massachusetts | Compositions and methods for improved gene editing |
| JP7579817B2 (ja) | 2019-07-03 | 2024-11-08 | インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド | Crisprで導入された二本鎖dna切断修復の同定、特徴付けおよび定量化 |
| US20210079387A1 (en) * | 2019-08-27 | 2021-03-18 | The Jackson Laboratory | Cleavage-resistant donor nucleic acids and methods of use |
| US20220275400A1 (en) * | 2019-08-30 | 2022-09-01 | The Trustees Of Columbia University In The City Of New York | Methods for scalable gene insertions |
| JP7762651B2 (ja) * | 2019-10-24 | 2025-10-30 | インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド | 修飾二重鎖ドナー鋳型 |
| US20230086199A1 (en) * | 2019-11-26 | 2023-03-23 | The Broad Institute, Inc. | Systems and methods for evaluating cas9-independent off-target editing of nucleic acids |
| JP2024513087A (ja) * | 2021-04-07 | 2024-03-21 | アストラゼネカ・アクチエボラーグ | 部位特異的改変のための組成物及び方法 |
| EP4083227A1 (en) * | 2021-04-29 | 2022-11-02 | 4basebio, S.L.U. | Linear dna with enhanced resistance against exonucleases |
| JP2024533311A (ja) * | 2021-09-08 | 2024-09-12 | フラッグシップ パイオニアリング イノベーションズ シックス,エルエルシー | ゲノムを調節するための方法及び組成物 |
| CN115029277B (zh) * | 2022-06-24 | 2023-08-22 | 四川省烟草公司凉山州公司 | 一株耐酸抗青枯病的假蕈状芽孢杆菌 |
| WO2025171070A1 (en) * | 2024-02-06 | 2025-08-14 | Integrated Dna Technologies, Inc. | Optimized spcas9 proteins for efficient genome editing in eukaryotic cells |
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| JP2001517939A (ja) * | 1997-03-14 | 2001-10-09 | ライフ テクノロジーズ,インコーポレイテッド | ペプチドによって増強されるトランスフェクション |
| US20140315985A1 (en) * | 2013-03-14 | 2014-10-23 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| JP2016509063A (ja) * | 2013-02-25 | 2016-03-24 | サンガモ バイオサイエンシーズ, インコーポレイテッド | ヌクレアーゼ媒介性遺伝子破壊を増強するための方法および組成物 |
| JP2016515401A (ja) * | 2013-04-16 | 2016-05-30 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. | ラットゲノムの標的改変 |
| JP2016539655A (ja) * | 2013-12-11 | 2016-12-22 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. | ゲノムの標的改変のための方法及び組成物 |
| WO2017066707A1 (en) * | 2015-10-14 | 2017-04-20 | Life Technologies Corporation | Ribonucleoprotein transfection agents |
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-
2018
- 2018-09-07 EP EP18779883.0A patent/EP3679143A1/en active Pending
- 2018-09-07 US US16/124,931 patent/US20190119701A1/en not_active Abandoned
- 2018-09-07 WO PCT/US2018/049966 patent/WO2019051237A1/en not_active Ceased
- 2018-09-07 CN CN202411245009.9A patent/CN119570864A/zh active Pending
- 2018-09-07 JP JP2020513724A patent/JP2020534812A/ja active Pending
- 2018-09-07 CN CN201880071907.3A patent/CN111315889A/zh active Pending
-
2023
- 2023-09-07 JP JP2023145587A patent/JP2023168355A/ja active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2001517939A (ja) * | 1997-03-14 | 2001-10-09 | ライフ テクノロジーズ,インコーポレイテッド | ペプチドによって増強されるトランスフェクション |
| JP2016509063A (ja) * | 2013-02-25 | 2016-03-24 | サンガモ バイオサイエンシーズ, インコーポレイテッド | ヌクレアーゼ媒介性遺伝子破壊を増強するための方法および組成物 |
| US20140315985A1 (en) * | 2013-03-14 | 2014-10-23 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| JP2016515401A (ja) * | 2013-04-16 | 2016-05-30 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. | ラットゲノムの標的改変 |
| JP2016539655A (ja) * | 2013-12-11 | 2016-12-22 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. | ゲノムの標的改変のための方法及び組成物 |
| WO2017066707A1 (en) * | 2015-10-14 | 2017-04-20 | Life Technologies Corporation | Ribonucleoprotein transfection agents |
Also Published As
| Publication number | Publication date |
|---|---|
| US20190119701A1 (en) | 2019-04-25 |
| WO2019051237A1 (en) | 2019-03-14 |
| JP2023168355A (ja) | 2023-11-24 |
| CN119570864A (zh) | 2025-03-07 |
| EP3679143A1 (en) | 2020-07-15 |
| CN111315889A (zh) | 2020-06-19 |
| US20240247286A1 (en) | 2024-07-25 |
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