CN119570864A - 提高同源重组的方法和其组合物 - Google Patents

提高同源重组的方法和其组合物 Download PDF

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Publication number
CN119570864A
CN119570864A CN202411245009.9A CN202411245009A CN119570864A CN 119570864 A CN119570864 A CN 119570864A CN 202411245009 A CN202411245009 A CN 202411245009A CN 119570864 A CN119570864 A CN 119570864A
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nucleic acid
protein
dna
binding
cell
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Chinese (zh)
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梁锡权
R·波特
L·彭
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Life Technologies Corp
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Life Technologies Corp
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
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    • C12N2800/00—Nucleic acids vectors
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    • C12N2800/00—Nucleic acids vectors
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    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source

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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202411245009.9A 2017-09-08 2018-09-07 提高同源重组的方法和其组合物 Pending CN119570864A (zh)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US201762555862P 2017-09-08 2017-09-08
US62/555,862 2017-09-08
US201762568661P 2017-10-05 2017-10-05
US62/568,661 2017-10-05
US201762574936P 2017-10-20 2017-10-20
US62/574,936 2017-10-20
US201862626792P 2018-02-06 2018-02-06
US62/626,792 2018-02-06
US201862717403P 2018-08-10 2018-08-10
US62/717,403 2018-08-10
CN201880071907.3A CN111315889A (zh) 2017-09-08 2018-09-07 提高同源重组的方法和其组合物
PCT/US2018/049966 WO2019051237A1 (en) 2017-09-08 2018-09-07 ENHANCED RECOMBINANT RECOMBINATION METHODS AND COMPOSITIONS THEREOF

Related Parent Applications (1)

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CN201880071907.3A Division CN111315889A (zh) 2017-09-08 2018-09-07 提高同源重组的方法和其组合物

Publications (1)

Publication Number Publication Date
CN119570864A true CN119570864A (zh) 2025-03-07

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CN202411245009.9A Pending CN119570864A (zh) 2017-09-08 2018-09-07 提高同源重组的方法和其组合物
CN201880071907.3A Pending CN111315889A (zh) 2017-09-08 2018-09-07 提高同源重组的方法和其组合物

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US (2) US20190119701A1 (https=)
EP (1) EP3679143A1 (https=)
JP (2) JP2020534812A (https=)
CN (2) CN119570864A (https=)
WO (1) WO2019051237A1 (https=)

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US20190119701A1 (en) 2019-04-25
US20240247286A1 (en) 2024-07-25
JP2020534812A (ja) 2020-12-03
CN111315889A (zh) 2020-06-19
EP3679143A1 (en) 2020-07-15
WO2019051237A1 (en) 2019-03-14
US12612650B2 (en) 2026-04-28
JP2023168355A (ja) 2023-11-24

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