JP2020532990A - 水素に富むc1含有基質を使用した代謝物生成方法およびシステム - Google Patents
水素に富むc1含有基質を使用した代謝物生成方法およびシステム Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Abstract
Description
本出願は、2017年9月8日に出願された米国仮特許出願第62/556,099号の利益を主張し、その内容は参照により本明細書に組み込まれる。
本発明は、接種リアクタおよび少なくとも1つのバイオリアクタを含む多段階ガス発酵プロセスを介する1つ以上の発酵生成物の生成方法に関する。具体的には、本発明は、COに富むC1含有ガス状基質が接種リアクタに供給されて、接種材料を生成する方法に関する。
別段の定めがない限り、本明細書全体で使用される以下の用語は、以下のように定義される。
CO2および/もしくはCOを生成物に変換すること、または
CO2および/もしくはCOを長期貯蔵に好適な物質に変換すること、または
CO2および/もしくはCOを長期貯蔵に好適な物質中に閉じ込めること、または
これらのプロセスの組み合わせのいずれかを指す。
接種装置および/またはバイオリアクタに供給されるC1含有ガス状基質の組成を制御することは、接種リアクタおよび後続のバイオリアクタの両方で、細胞増殖、生成物選択性、および安定性を促進するのに特に有用であることが判明した。好ましくは、C1含有基質は、接種リアクタに供給される前に組成制御され、1つ以上の下流のリアクタに供給するための接種材料を生成する。好ましくは、接種リアクタは、液体栄養培地中の1つ以上のC1固定微生物の培養物を含み、組成制御C1含有ガス状基質を受容して、発酵を介して接種材料を生成し得る。
場合によっては、必要なガス量によって、コストのためにボンベに充填されたガスの使用が禁止され得る。したがって、H2に富むC1含有ガス状基質が処理されて、基質から水素の少なくとも一部分を除去し、COに富むC1含有ガス状基質を生成することが好ましい。H2に富むC1含有ガス状基質を処理するための好適な方法としては、膜分離技術、および圧力スイング吸着技術を挙げることができるが、これらに限定されない。
COに富むC1含有ガス状基質を提供する代替の方法は、CO2電気分解の使用を介することである。CO2電気分解プロセスは、CO2供給原料をCOおよびO2に変換する。接種装置にCOに富む流れを提供するためのCO2電気分解プロセスの使用は、O2に富む流れに加えてCO2に富む流れを含む工業的現場における、関心対象であり得る。加えて、CO2に富む接種リアクタおよび/またはバイオリアクタシステムからの排ガスは、CO2電気分解ユニットへの供給原料として使用され得るとさらに考えられる。
この実施例は、68体積%のH2、3.8体積%のCO、26体積%のCO2、および1体積%のN2を、18:1のH2:COモル比で含むガス状基質が提供された2つのリアクタの比較性能を実証する。2つのリアクタの操作パラメーターの唯一の違いは、各リアクタの接種材料が生成される条件であった。図6aおよび図6bは、COが豊富な条件下で生成された接種材料を受容した第1のバイオリアクタの代謝物およびガスのプロファイルを示す。図7aおよび図7bは、H2が豊富な条件下で生成された接種材料を受容した第2のバイオリアクタの代謝物およびガスのプロファイルを示す。どちらのリアクタも、同様の効率でH2、CO、およびCO2を消費するが、H2に富む接種リアクタから接種材料を受容したリアクタ(図7a)は、エタノールに対する選択性が低減した。
この実施例は、異なるガス条件下で作動する接種リアクタから接種材料が提供されたリアクタの比較性能を実証する。図8aおよび図8bは、以下のガス組成、48体積%のH2、40体積%のCO、2体積%のCO2、および10体積%のN2を用いて生成された接種材料から受容された培養物が接種された発酵の代謝物およびガスのプロファイルを示す。図9aおよび図9bは、COが豊富な条件下で生成された接種材料から受容された培養物が接種された発酵の代謝物およびガスのプロファイルを示す。COに富むガス接種リアクタ(図9a)から供給された、リアクタによって実証されたエタノール選択性は、H2に富む接種リアクタ(図8a)から接種材料を受容したリアクタよりもはるかに高い。
Claims (23)
- 1つ以上の発酵生成物の生成方法であって、
a.COに富むC1含有ガス状基質を、1つ以上のC1固定微生物の培養物を含有する液体栄養培地を含む接種リアクタに提供することと、
b.前記COに富むC1含有ガス状基質を発酵させて、接種材料を生成することと、
c.前記接種材料の少なくとも一部分を、バイオリアクタシステムに送ることであって、前記バイオリアクタシステムが、液体栄養培地中に1つ以上のC1固定微生物の培養物を含有する少なくとも1つのバイオリアクタを備える、送ることと、
d.H2に富むC1含有ガス状基質を、前記バイオリアクタシステムに送ることと、
e.前記H2に富むC1含有ガス状基質を発酵させて、少なくとも1つの発酵生成物を生成することと、を含む、方法。 - 前記COに富むC1含有ガス状基質が、1:1未満のH2:COモル比で、H2を含む、請求項1に記載の方法。
- 前記COに富むC1含有ガス状基質が、0.5:1未満のH2:COモル比で、H2を含む、請求項1に記載の方法。
- 前記COに富むC1含有ガス状基質が、0.02:1〜1:1のH2:COモル比で、H2を含む、請求項1に記載の方法。
- 前記H2に富むC1含有ガス状基質が、少なくとも1.1:1のH2:COモル比で、H2を含む、請求項1に記載の方法。
- 前記H2に富むC1含有ガス状基質が、1.1:1〜6:1のH2:COモル比で、H2を含む、請求項1に記載の方法。
- 前記C1固定微生物が、カルボキシド栄養性細菌である、請求項1記載の方法。
- 前記カルボキシド栄養性細菌が、ムーレラ(Moorella)、クロストリジウム(Clostridium)、ルミノコッカス(Ruminococcus)、アセトバクテリウム(Acetobacterium)、ユーバクテリウム(Eubacterium)、ブチリバクテリウム(Butyribacterium)、オキソバクター(Oxobacter)、メタノサルシナ(Methanosarcina)、およびデスルホトマキュラム(Desulfotomaculum)からなる群から選択される、請求項7に記載の方法。
- 前記カルボキシド栄養性細菌が、クロストリジウムオートエタノゲヌム(Clostridium autoethanogenum)である、請求項7に記載の方法。
- 前記バイオリアクタシステムが、1つ以上の二次バイオリアクタに連結された1つ以上の一次バイオリアクタを備える、請求項1に記載の方法。
- 1つ以上の発酵生成物の生成方法であって、
a.C1含有ガス状基質の少なくとも一部分を、接種リアクタに、かつ前記C1含有ガス状基質の少なくとも一部分を、バイオリアクタに送ることと、
b.前記C1含有ガス状基質を、前記接種リアクタ内で発酵させて、接種材料を生成することと、
c.前記接種材料の少なくとも一部分を、少なくとも1つのバイオリアクタに送ることと、
d.前記C1含有ガス状基質を、前記バイオリアクタ内で発酵させて、少なくとも1つの発酵生成物を生成することと、を含み、
e.前記接種リアクタに送られる前記C1含有ガス状基質が、前記接種リアクタに送られる前に、少なくとも1つのH2除去プロセスに供される、方法。 - 前記接種リアクタに送られる前記C1含有ガス状基質が、1:1未満のH2:COモル比で、H2を含む、請求項11に記載の方法。
- 前記接種リアクタに送られる前記C1含有ガス状基質が、0.8:1未満のH2:COモル比で、H2を含む、請求項11に記載の方法。
- 前記接種リアクタに送られる前記C1含有ガス状基質が、0.5:1未満のH2:COモル比で、H2を含む、請求項11に記載の方法。
- 前記接種リアクタに送られる前記C1含有ガス状基質が、0.02:1〜1:1のH2:COモル比で、H2を含む、請求項11に記載の方法。
- 前記H2除去プロセスが、少なくとも1つの圧力スイング吸着プロセスを含む、請求項11に記載の方法。
- 前記H2除去プロセスが、少なくとも1つの膜分離モジュールを含む、請求項11に記載の方法。
- 前記C1含有ガス状基質の少なくとも一部分が、工業的供給源から誘導される、請求項11に記載の方法。
- 前記工業的供給源が、炭水化物発酵、ガス発酵、セメント製造、パルプ製紙、製鋼、石油精製および関連プロセス、石油化学製造、コークス製造、嫌気性または好気性消化、合成ガス、天然ガスの抽出、石油の抽出、アルミニウム、銅、および/または合金鉄の製造および/または精製のための冶金プロセス、地質学的貯留池、ならびに触媒プロセスからなる群から選択される、請求項18に記載の方法。
- 少なくとも1つの発酵生成物が、エタノール、アセテート、ブタノール、ブチレート、2,3−ブタンジオール、1,3−ブタンジオール、ラクテート、ブテン、ブタジエン、メチルエチルケトン、エチレン、アセトン、イソプロパノール、脂質、3−ヒドロキシプロピオネート、イソプレン、脂肪酸、2−ブタノール、1,2−プロパンジオール、1−プロパノール、モノエチレングリコール、イソブテン、およびC6〜C14アルコールからなる群から選択される、請求項11に記載の方法。
- 前記1つ以上の発酵生成物が、ディーゼル燃料、ジェット燃料、ガソリン、プロピレン、ナイロン6−6、ゴム、および/または樹脂のうちの少なくとも1つの成分にさらに変換される、請求項20に記載の方法。
- 少なくとも1つの発酵生成物が、微生物バイオマスである、請求項11に記載の方法。
- 前記微生物バイオマスが処理されて、動物用飼料の少なくとも1つの成分を生成する、請求項22に記載の方法。
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CN117280040A (zh) * | 2021-04-09 | 2023-12-22 | 朗泽科技有限公司 | 控制气体发酵平台以提高二氧化碳转化为产物的方法 |
WO2022217282A1 (en) * | 2021-04-09 | 2022-10-13 | Lanzatech, Inc. | Process and apparatus for providing a feedstock |
IT202100020819A1 (it) | 2021-08-02 | 2023-02-02 | Nextchem S P A | Processo ed apparato per la produzione di bioetanolo senza emissioni di co2 mediante conversione di syngas ottenuto dalla conversione termica ad alta temperatura di rifiuti |
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JP7326252B2 (ja) | 2023-08-15 |
ES2926336T3 (es) | 2022-10-25 |
MY193827A (en) | 2022-10-27 |
EA202090616A1 (ru) | 2020-06-10 |
KR20200041381A (ko) | 2020-04-21 |
EP3679149B1 (en) | 2022-06-29 |
JP2023145652A (ja) | 2023-10-11 |
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US20190078121A1 (en) | 2019-03-14 |
AU2018329846B2 (en) | 2024-01-11 |
PT3679149T (pt) | 2022-09-05 |
CA3125247A1 (en) | 2019-03-14 |
EP3679149A1 (en) | 2020-07-15 |
TW202311521A (zh) | 2023-03-16 |
ZA202001312B (en) | 2021-08-25 |
US10808263B2 (en) | 2020-10-20 |
TW201920645A (zh) | 2019-06-01 |
CN111051517A (zh) | 2020-04-21 |
CA3074292A1 (en) | 2019-03-14 |
TWI787340B (zh) | 2022-12-21 |
CN111051517B (zh) | 2023-10-27 |
CN117551705A (zh) | 2024-02-13 |
CA3074292C (en) | 2021-08-24 |
CA3125247C (en) | 2023-08-01 |
US11466295B2 (en) | 2022-10-11 |
BR112020004591A2 (pt) | 2020-09-08 |
SG11202001716XA (en) | 2020-03-30 |
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