JP2020532967A5 - - Google Patents
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- JP2020532967A5 JP2020532967A5 JP2020511253A JP2020511253A JP2020532967A5 JP 2020532967 A5 JP2020532967 A5 JP 2020532967A5 JP 2020511253 A JP2020511253 A JP 2020511253A JP 2020511253 A JP2020511253 A JP 2020511253A JP 2020532967 A5 JP2020532967 A5 JP 2020532967A5
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- 230000035772 mutation Effects 0.000 claims 12
- 102000004169 proteins and genes Human genes 0.000 claims 12
- 108090000623 proteins and genes Proteins 0.000 claims 12
- 210000004027 cell Anatomy 0.000 claims 10
- 108020004414 DNA Proteins 0.000 claims 8
- 108020001507 fusion proteins Proteins 0.000 claims 7
- 102000037865 fusion proteins Human genes 0.000 claims 7
- 238000000034 method Methods 0.000 claims 6
- 102000053602 DNA Human genes 0.000 claims 5
- 102000004190 Enzymes Human genes 0.000 claims 5
- 108090000790 Enzymes Proteins 0.000 claims 5
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 claims 4
- 102000055025 Adenosine deaminases Human genes 0.000 claims 4
- 102100026846 Cytidine deaminase Human genes 0.000 claims 4
- 108010031325 Cytidine deaminase Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 101710142939 Adenosine deaminase 1 Proteins 0.000 claims 2
- 108091033409 CRISPR Proteins 0.000 claims 2
- 102000000311 Cytosine Deaminase Human genes 0.000 claims 2
- 108010080611 Cytosine Deaminase Proteins 0.000 claims 2
- 108020005004 Guide RNA Proteins 0.000 claims 2
- 101710160287 Heterochromatin protein 1 Proteins 0.000 claims 2
- 108010033040 Histones Proteins 0.000 claims 2
- 102000016397 Methyltransferase Human genes 0.000 claims 2
- 108060004795 Methyltransferase Proteins 0.000 claims 2
- 241000193996 Streptococcus pyogenes Species 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 claims 2
- 230000003584 silencer Effects 0.000 claims 2
- 101710199622 tRNA-specific adenosine deaminase Proteins 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 230000037426 transcriptional repression Effects 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 108010029988 AICDA (activation-induced cytidine deaminase) Proteins 0.000 claims 1
- 108010079649 APOBEC-1 Deaminase Proteins 0.000 claims 1
- 108010004483 APOBEC-3G Deaminase Proteins 0.000 claims 1
- 102100036664 Adenosine deaminase Human genes 0.000 claims 1
- 102100025976 Adenosine deaminase 2 Human genes 0.000 claims 1
- 102100040397 C->U-editing enzyme APOBEC-1 Human genes 0.000 claims 1
- 102100040399 C->U-editing enzyme APOBEC-2 Human genes 0.000 claims 1
- 108010051109 Cell-Penetrating Peptides Proteins 0.000 claims 1
- 102000020313 Cell-Penetrating Peptides Human genes 0.000 claims 1
- 102220605874 Cytosolic arginine sensor for mTORC1 subunit 2_D10A_mutation Human genes 0.000 claims 1
- 102100040263 DNA dC->dU-editing enzyme APOBEC-3A Human genes 0.000 claims 1
- 102100040262 DNA dC->dU-editing enzyme APOBEC-3B Human genes 0.000 claims 1
- 102100040261 DNA dC->dU-editing enzyme APOBEC-3C Human genes 0.000 claims 1
- 102100040264 DNA dC->dU-editing enzyme APOBEC-3D Human genes 0.000 claims 1
- 102100040266 DNA dC->dU-editing enzyme APOBEC-3F Human genes 0.000 claims 1
- 102100038076 DNA dC->dU-editing enzyme APOBEC-3G Human genes 0.000 claims 1
- 102100029791 Double-stranded RNA-specific adenosine deaminase Human genes 0.000 claims 1
- 102100038191 Double-stranded RNA-specific editase 1 Human genes 0.000 claims 1
- 102100024692 Double-stranded RNA-specific editase B2 Human genes 0.000 claims 1
- 102220502946 Geranylgeranyl transferase type-2 subunit alpha_D10N_mutation Human genes 0.000 claims 1
- 108010068250 Herpes Simplex Virus Protein Vmw65 Proteins 0.000 claims 1
- 101000720051 Homo sapiens Adenosine deaminase 2 Proteins 0.000 claims 1
- 101000964322 Homo sapiens C->U-editing enzyme APOBEC-2 Proteins 0.000 claims 1
- 101000964378 Homo sapiens DNA dC->dU-editing enzyme APOBEC-3A Proteins 0.000 claims 1
- 101000964385 Homo sapiens DNA dC->dU-editing enzyme APOBEC-3B Proteins 0.000 claims 1
- 101000964383 Homo sapiens DNA dC->dU-editing enzyme APOBEC-3C Proteins 0.000 claims 1
- 101000964382 Homo sapiens DNA dC->dU-editing enzyme APOBEC-3D Proteins 0.000 claims 1
- 101000964377 Homo sapiens DNA dC->dU-editing enzyme APOBEC-3F Proteins 0.000 claims 1
- 101000865408 Homo sapiens Double-stranded RNA-specific adenosine deaminase Proteins 0.000 claims 1
- 101000742223 Homo sapiens Double-stranded RNA-specific editase 1 Proteins 0.000 claims 1
- 101000686486 Homo sapiens Double-stranded RNA-specific editase B2 Proteins 0.000 claims 1
- 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 claims 1
- 101000653360 Homo sapiens Methylcytosine dioxygenase TET1 Proteins 0.000 claims 1
- 101000799048 Homo sapiens Probable inactive tRNA-specific adenosine deaminase-like protein 3 Proteins 0.000 claims 1
- 101000800426 Homo sapiens Putative C->U-editing enzyme APOBEC-4 Proteins 0.000 claims 1
- 101000657352 Homo sapiens Transcriptional adapter 2-alpha Proteins 0.000 claims 1
- 101000799160 Homo sapiens tRNA-specific adenosine deaminase 1 Proteins 0.000 claims 1
- 101000799057 Homo sapiens tRNA-specific adenosine deaminase 2 Proteins 0.000 claims 1
- 102100021299 Methyl-CpG-binding domain protein 2 Human genes 0.000 claims 1
- 102100030819 Methylcytosine dioxygenase TET1 Human genes 0.000 claims 1
- 108010057466 NF-kappa B Proteins 0.000 claims 1
- 102000003945 NF-kappa B Human genes 0.000 claims 1
- 101710141454 Nucleoprotein Proteins 0.000 claims 1
- 102100034006 Probable inactive tRNA-specific adenosine deaminase-like protein 3 Human genes 0.000 claims 1
- 102100033091 Putative C->U-editing enzyme APOBEC-4 Human genes 0.000 claims 1
- 101150023114 RNA1 gene Proteins 0.000 claims 1
- 101710122931 Replication and transcription activator Proteins 0.000 claims 1
- 102000004389 Ribonucleoproteins Human genes 0.000 claims 1
- 108010081734 Ribonucleoproteins Proteins 0.000 claims 1
- 102100022433 Single-stranded DNA cytosine deaminase Human genes 0.000 claims 1
- 102000005747 Transcription Factor RelA Human genes 0.000 claims 1
- 108010031154 Transcription Factor RelA Proteins 0.000 claims 1
- 102000004357 Transferases Human genes 0.000 claims 1
- 108090000992 Transferases Proteins 0.000 claims 1
- -1 VP64 Proteins 0.000 claims 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 230000003197 catalytic effect Effects 0.000 claims 1
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical class O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 210000001671 embryonic stem cell Anatomy 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 230000017156 mRNA modification Effects 0.000 claims 1
- 210000001161 mammalian embryo Anatomy 0.000 claims 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims 1
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
- 102100034113 tRNA-specific adenosine deaminase 1 Human genes 0.000 claims 1
- 102100034045 tRNA-specific adenosine deaminase 2 Human genes 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024080681A JP2024112895A (ja) | 2017-08-23 | 2024-05-17 | 変更されたＰＡＭ特異性を有する遺伝子操作されたＣＲＩＳＰＲ－Ｃａｓ９ヌクレアーゼ |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762549303P | 2017-08-23 | 2017-08-23 | |
| US62/549,303 | 2017-08-23 | ||
| US201862641687P | 2018-03-12 | 2018-03-12 | |
| US62/641,687 | 2018-03-12 | ||
| PCT/US2018/047577 WO2019040650A1 (en) | 2017-08-23 | 2018-08-22 | GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES HAVING MODIFIED PAM SPECIFICITY |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024080681A Division JP2024112895A (ja) | 2017-08-23 | 2024-05-17 | 変更されたＰＡＭ特異性を有する遺伝子操作されたＣＲＩＳＰＲ－Ｃａｓ９ヌクレアーゼ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020532967A JP2020532967A (ja) | 2020-11-19 |
| JP2020532967A5 true JP2020532967A5 (enExample) | 2021-09-30 |
| JP7581046B2 JP7581046B2 (ja) | 2024-11-12 |
Family
ID=65439890
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020511253A Active JP7581046B2 (ja) | 2017-08-23 | 2018-08-22 | 変更されたＰＡＭ特異性を有する遺伝子操作されたＣＲＩＳＰＲ－Ｃａｓ９ヌクレアーゼ |
| JP2024080681A Pending JP2024112895A (ja) | 2017-08-23 | 2024-05-17 | 変更されたＰＡＭ特異性を有する遺伝子操作されたＣＲＩＳＰＲ－Ｃａｓ９ヌクレアーゼ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024080681A Pending JP2024112895A (ja) | 2017-08-23 | 2024-05-17 | 変更されたＰＡＭ特異性を有する遺伝子操作されたＣＲＩＳＰＲ－Ｃａｓ９ヌクレアーゼ |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US11286468B2 (enExample) |
| EP (1) | EP3672612A4 (enExample) |
| JP (2) | JP7581046B2 (enExample) |
| CN (2) | CN111278450B (enExample) |
| AU (3) | AU2018320865B2 (enExample) |
| BR (1) | BR112020003596A2 (enExample) |
| CA (1) | CA3073448A1 (enExample) |
| WO (1) | WO2019040650A1 (enExample) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9890364B2 (en) | 2012-05-29 | 2018-02-13 | The General Hospital Corporation | TAL-Tet1 fusion proteins and methods of use thereof |
| EP3919621A1 (en) | 2014-06-23 | 2021-12-08 | The General Hospital Corporation | Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq) |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| JP6817215B2 (ja) | 2015-03-03 | 2021-01-20 | ザ ジェネラル ホスピタル コーポレイション | 変更ＰＡＭ特異性を有する遺伝子操作ＣＲＩＳＰＲ−Ｃａｓ９ヌクレアーゼ |
| CN108350453A (zh) | 2015-09-11 | 2018-07-31 | 通用医疗公司 | 核酸酶dsb的完全查询和测序(find-seq) |
| US11168313B2 (en) | 2016-07-26 | 2021-11-09 | The General Hospital Corporation | Variants of CRISPR from Prevotella and Francisella 1 (Cpf1) |
| WO2018119010A1 (en) | 2016-12-19 | 2018-06-28 | Editas Medicine, Inc. | Assessing nuclease cleavage |
| EP3565907B1 (en) | 2017-01-06 | 2022-05-04 | Editas Medicine, Inc. | Methods of assessing nuclease cleavage |
| ES2992912T3 (en) | 2017-04-21 | 2024-12-19 | Massachusetts Gen Hospital | Variants of cpf1 (cas12a) with altered pam specificity |
| US11499151B2 (en) | 2017-04-28 | 2022-11-15 | Editas Medicine, Inc. | Methods and systems for analyzing guide RNA molecules |
| AU2018273986A1 (en) | 2017-05-25 | 2019-12-12 | The General Hospital Corporation | Bipartite base editor (BBE) architectures and type-II-C-Cas9 zinc finger editing |
| JP7518620B2 (ja) | 2017-06-09 | 2024-07-18 | エディタス・メディシン，インコーポレイテッド | 操作されたｃａｓ９ヌクレアーゼ |
| WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
| CA3073448A1 (en) | 2017-08-23 | 2019-02-28 | The General Hospital Corporation | Engineered crispr-cas9 nucleases with altered pam specificity |
| EP3781585A4 (en) | 2018-04-17 | 2022-01-26 | The General Hospital Corporation | SENSITIVE IN VITRO TESTS FOR SUBSTRATE PREFERENCES AND SITE FOR NUCLEIC ACID BINDERS, MODIFIERS AND CLEAVAGE AGENTS |
| AU2019265019B2 (en) | 2018-05-11 | 2025-11-06 | Beam Therapeutics Inc. | Methods of substituting pathogenic amino acids using programmable base editor systems |
| KR20250133482A (ko) * | 2018-05-11 | 2025-09-05 | 빔 테라퓨틱스, 인크. | 프로그래밍가능한 염기 편집기 시스템을 이용하여 단일 뉴클레오티드 다형성을 편집하는 방법 |
| AU2019291918B2 (en) | 2018-06-29 | 2025-06-12 | Editas Medicine, Inc. | Synthetic guide molecules, compositions and methods relating thereto |
| AU2019336245B2 (en) | 2018-09-07 | 2026-02-26 | Beam Therapeutics Inc. | Compositions and methods for delivering a nucleobase editing system |
| EP3850088A4 (en) | 2018-09-07 | 2023-07-19 | Beam Therapeutics, Inc. | Compositions and methods for improving base editing |
| CN114040977B (zh) | 2019-01-31 | 2024-09-03 | 比姆医疗股份有限公司 | 非靶脱氨反应减低的核碱基编辑器和用于定性核碱基编辑器的测定 |
| EP3921417A4 (en) | 2019-02-04 | 2022-11-09 | The General Hospital Corporation | VARIANTS OF ADENINE DNA BASES WITH REDUCED OFF-TARGET RNA EDIT |
| AU2020223314A1 (en) | 2019-02-13 | 2021-08-12 | Beam Therapeutics Inc. | Compositions and methods for treating alpha-1 antitrypsin deficiency |
| SG11202108346WA (en) | 2019-02-13 | 2021-08-30 | Beam Therapeutics Inc | Modified immune cells having adenosine deaminase base editors for modifying a nucleobase in a target sequence |
| CN112266418A (zh) * | 2019-07-08 | 2021-01-26 | 中国科学院微生物研究所 | 改进的基因组编辑系统及其应用 |
| CN112280771A (zh) * | 2019-07-10 | 2021-01-29 | 中国科学院遗传与发育生物学研究所 | 双功能基因组编辑系统及其用途 |
| EP4021945A4 (en) * | 2019-08-30 | 2023-11-15 | The General Hospital Corporation | Combinatorial adenine and cytosine dna base editors |
| AU2020345830A1 (en) * | 2019-09-09 | 2022-03-24 | Beam Therapeutics Inc. | Novel CRISPR enzymes, methods, systems and uses thereof |
| CN115279398B (zh) | 2019-09-27 | 2025-07-04 | 比姆医疗股份有限公司 | 治疗液态癌症的组合物和方法 |
| US12497609B2 (en) | 2019-10-14 | 2025-12-16 | Yonsei University Biohealth Technology Holdings. Inc. | Protospacer adjacent motif sequence and method for modifying target nucleic acid in genome of cell by using same |
| WO2021151085A2 (en) | 2020-01-24 | 2021-07-29 | The General Hospital Corporation | Crispr-cas enzymes with enhanced on-target activity |
| WO2021151073A2 (en) * | 2020-01-24 | 2021-07-29 | The General Hospital Corporation | Unconstrained genome targeting with near-pamless engineered crispr-cas9 variants |
| EP4114937A4 (en) | 2020-03-04 | 2024-09-04 | Flagship Pioneering Innovations VI, LLC | METHODS AND COMPOSITIONS FOR MODULATING A GENOME |
| US12576151B2 (en) | 2020-09-25 | 2026-03-17 | Beam Therapeutics Inc. | Fratricide resistant modified immune cells and methods of using the same |
| CN112430613A (zh) * | 2020-12-08 | 2021-03-02 | 安徽省农业科学院水稻研究所 | 一种宽编辑范围的SpG基因及其应用 |
| WO2022152746A1 (en) | 2021-01-13 | 2022-07-21 | Alia Therapeutics Srl | K526d cas9 variants and applications thereof |
| KR20260037664A (ko) | 2021-09-08 | 2026-03-18 | 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 | 게놈을 조절하기 위한 방법 및 조성물 |
| WO2023039435A2 (en) | 2021-09-08 | 2023-03-16 | Flagship Pioneering Innovations Vi, Llc | Pah-modulating compositions and methods |
| CN116286736B (zh) * | 2022-01-17 | 2026-05-01 | 四川大学 | 一种具有扩大的pam识别范围的融合蛋白及其应用 |
| WO2025087413A1 (zh) * | 2023-10-25 | 2025-05-01 | 上海津曼特生物科技有限公司 | 靶向ttr的基因编辑组合物 |
| WO2025158391A2 (en) * | 2024-01-24 | 2025-07-31 | The General Hospital Corporation | Generation of custom crispr-cas9 pam variant enzymes |
| CN118048343B (zh) * | 2024-03-01 | 2025-01-28 | 上海交通大学 | Cas9核酸酶及其制备方法和用途 |
| CN120005854B (zh) * | 2025-04-18 | 2025-08-05 | 广州瑞风生物科技有限公司 | Cas9突变体、基因编辑系统及应用 |
| CN120665840A (zh) * | 2025-05-30 | 2025-09-19 | 浙江大学 | Cas9蛋白突变体及其应用 |
Family Cites Families (106)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005090607A1 (en) | 2004-03-08 | 2005-09-29 | Rubicon Genomics, Inc. | Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation |
| CA2615323A1 (en) | 2005-06-06 | 2007-12-21 | 454 Life Sciences Corporation | Paired end sequencing |
| ATE510930T1 (de) | 2005-08-02 | 2011-06-15 | Rubicon Genomics Inc | Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion |
| NZ597299A (en) | 2005-08-26 | 2013-03-28 | Dupont Nutrition Biosci Aps | Use of a cas gene in combination with CRISPR repeats for modulating resistance in a cell |
| WO2007059041A2 (en) | 2005-11-11 | 2007-05-24 | Pfizer, Inc. | Combinations and methods of using an immunomodulatory oligodeoxynucleotide |
| PT2126130E (pt) | 2007-03-02 | 2015-08-03 | Dupont Nutrition Biosci Aps | Culturas com resistência melhorada a fagos |
| WO2009027978A1 (en) | 2007-08-30 | 2009-03-05 | Hadasit Medical Research Services & Development Ltd. | NUCLEIC ACID SEQUENCES COMPRISING NF-ϰB BINDING SITE WITHIN O(6)-METHYLGUANINE-DNA-METHYLTRANSFERASE (MGMT) PROMOTER REGION AND USES THEREOF FOR THE TREATMENT OF CANCER AND IMMUNE-RELATED DISORDERS |
| US8029993B2 (en) | 2008-04-30 | 2011-10-04 | Population Genetics Technologies Ltd. | Asymmetric adapter library construction |
| WO2010011961A2 (en) | 2008-07-25 | 2010-01-28 | University Of Georgia Research Foundation, Inc. | Prokaryotic rnai-like system and methods of use |
| US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| US20100076057A1 (en) | 2008-09-23 | 2010-03-25 | Northwestern University | TARGET DNA INTERFERENCE WITH crRNA |
| US9404098B2 (en) | 2008-11-06 | 2016-08-02 | University Of Georgia Research Foundation, Inc. | Method for cleaving a target RNA using a Cas6 polypeptide |
| EP2367938B1 (en) | 2008-12-12 | 2014-06-11 | DuPont Nutrition Biosciences ApS | Genetic cluster of strains of streptococcus thermophilus having unique rheological properties for dairy fermentation |
| JP5387839B2 (ja) | 2009-09-04 | 2014-01-15 | アイシン・エィ・ダブリュ株式会社 | ナビゲーション装置、ナビゲーション方法、及びナビゲーションプログラム |
| US9023769B2 (en) | 2009-11-30 | 2015-05-05 | Complete Genomics, Inc. | cDNA library for nucleic acid sequencing |
| US9493827B2 (en) | 2010-01-14 | 2016-11-15 | Deutsches Krebsforschungszentrum | Determination of in vivo DNA double-strand break localization and application thereof |
| JP2013518602A (ja) | 2010-02-09 | 2013-05-23 | サンガモ バイオサイエンシーズ， インコーポレイテッド | 部分的に一本鎖のドナー分子による標的化ゲノム改変 |
| US10087431B2 (en) | 2010-03-10 | 2018-10-02 | The Regents Of The University Of California | Methods of generating nucleic acid fragments |
| EA024121B9 (ru) | 2010-05-10 | 2017-01-30 | Дзе Реджентс Ов Дзе Юниверсити Ов Калифорния | Композиции эндорибонуклеаз и способы их использования |
| AU2011325956B2 (en) | 2010-11-12 | 2016-07-14 | The General Hospital Corporation | Polycomb-associated non-coding RNAs |
| WO2012164565A1 (en) | 2011-06-01 | 2012-12-06 | Yeda Research And Development Co. Ltd. | Compositions and methods for downregulating prokaryotic genes |
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| WO2013078470A2 (en) | 2011-11-22 | 2013-05-30 | MOTIF, Active | Multiplex isolation of protein-associated nucleic acids |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| EP2847353B1 (en) | 2012-05-10 | 2022-01-19 | The General Hospital Corporation | Methods for determining a nucleotide sequence |
| LT3401400T (lt) | 2012-05-25 | 2019-06-10 | The Regents Of The University Of California | Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui |
| JP6181751B2 (ja) | 2012-06-18 | 2017-08-16 | ニューゲン テクノロジーズ， インコーポレイテッド | 望まれない核酸配列のネガティブ選択のための組成物および方法 |
| US20140024542A1 (en) | 2012-07-17 | 2014-01-23 | Counsyl, Inc. | Methods and compositions for enrichment of target polynucleotides |
| AU2012385961B9 (en) | 2012-07-24 | 2017-05-18 | Natera, Inc. | Highly multiplex PCR methods and compositions |
| WO2014071070A1 (en) | 2012-11-01 | 2014-05-08 | Pacific Biosciences Of California, Inc. | Compositions and methods for selection of nucleic acids |
| CN104903466B (zh) | 2012-11-05 | 2016-11-23 | 鲁比康基因组学公司 | 条形编码核酸 |
| EP2931898B1 (en) | 2012-12-12 | 2016-03-09 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| EP2896697B1 (en) | 2012-12-12 | 2015-09-02 | The Broad Institute, Inc. | Engineering of systems, methods and optimized guide compositions for sequence manipulation |
| JP2016504026A (ja) | 2012-12-12 | 2016-02-12 | ザ・ブロード・インスティテュート・インコーポレイテッド | 配列操作のための系、方法および最適化ガイド組成物のエンジニアリング |
| US20140310830A1 (en) | 2012-12-12 | 2014-10-16 | Feng Zhang | CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| WO2014093701A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| IL239317B (en) | 2012-12-12 | 2022-07-01 | Broad Inst Inc | Providing, engineering and optimizing systems, methods and compositions for sequence manipulation and therapeutic applications |
| ES2576126T3 (es) | 2012-12-12 | 2016-07-05 | The Broad Institute, Inc. | Modificación por tecnología genética y optimización de sistemas, métodos y composiciones enzimáticas mejorados para la manipulación de secuencias |
| CN114634950A (zh) | 2012-12-12 | 2022-06-17 | 布罗德研究所有限公司 | 用于序列操纵的crispr-cas组分系统、方法以及组合物 |
| AU2013359146B2 (en) | 2012-12-13 | 2017-12-07 | Corteva Agriscience Llc | DNA detection methods for site specific nuclease activity |
| CA2897932A1 (en) | 2013-01-14 | 2014-07-17 | Recombinetics, Inc. | Hornless livestock |
| US20140212869A1 (en) | 2013-01-25 | 2014-07-31 | Agilent Technologies, Inc. | Nucleic Acid Proximity Assay Involving the Formation of a Three-way junction |
| CA2900338C (en) | 2013-02-07 | 2024-11-05 | The General Hospital Corporation | TRANSCRIPTIONAL ACTIVATORS TALE |
| EP2958996B1 (en) | 2013-02-25 | 2019-10-16 | Sangamo Therapeutics, Inc. | Methods and compositions for enhancing nuclease-mediated gene disruption |
| SG11201507378UA (en) | 2013-03-14 | 2015-10-29 | Caribou Biosciences Inc | Compositions and methods of nucleic acid-targeting nucleic acids |
| US20140273230A1 (en) | 2013-03-15 | 2014-09-18 | Sigma-Aldrich Co., Llc | Crispr-based genome modification and regulation |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| US11332719B2 (en) | 2013-03-15 | 2022-05-17 | The Broad Institute, Inc. | Recombinant virus and preparations thereof |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US20140349400A1 (en) | 2013-03-15 | 2014-11-27 | Massachusetts Institute Of Technology | Programmable Modification of DNA |
| US10378027B2 (en) | 2013-03-15 | 2019-08-13 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| RU2723130C2 (ru) | 2013-04-05 | 2020-06-08 | ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи | Способы и композиции для встраивания экзогенной последовательности в геном растений |
| DK2986729T3 (en) | 2013-04-16 | 2018-10-29 | Regeneron Pharma | TARGETED MODIFICATION OF ROOT THROUGH |
| CA2910427C (en) | 2013-05-10 | 2024-02-20 | Sangamo Biosciences, Inc. | Delivery methods and compositions for nuclease-mediated genome engineering |
| EP3004339B1 (en) | 2013-05-29 | 2021-07-07 | Cellectis | New compact scaffold of cas9 in the type ii crispr system |
| WO2014204724A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
| JP6665088B2 (ja) | 2013-06-17 | 2020-03-13 | ザ・ブロード・インスティテュート・インコーポレイテッド | 配列操作のための最適化されたＣＲＩＳＰＲ−Ｃａｓ二重ニッカーゼ系、方法および組成物 |
| JP6482546B2 (ja) | 2013-07-19 | 2019-03-13 | ラリクス・バイオサイエンス・リミテッド・ライアビリティ・カンパニーＬａｒｉｘ Ｂｉｏｓｃｉｅｎｃｅ， Ｌｌｃ | 二重対立遺伝子ノックアウトを生成するための方法および組成物 |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| WO2015074017A1 (en) | 2013-11-18 | 2015-05-21 | Rubicon Genomics | Degradable adaptors for background reduction |
| WO2015089486A2 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems |
| WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
| WO2015089473A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation |
| DK3604544T3 (en) | 2014-01-31 | 2022-02-14 | Swift Biosciences Inc | Improved methods for processing dna substrates |
| EP3919621A1 (en) | 2014-06-23 | 2021-12-08 | The General Hospital Corporation | Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq) |
| AU2016206870B2 (en) | 2015-01-12 | 2022-02-17 | Massachusetts Institute Of Technology | Gene editing through microfluidic delivery |
| MA41349A (fr) | 2015-01-14 | 2017-11-21 | Univ Temple | Éradication de l'herpès simplex de type i et d'autres virus de l'herpès associés guidée par arn |
| JP6817215B2 (ja) * | 2015-03-03 | 2021-01-20 | ザ ジェネラル ホスピタル コーポレイション | 変更ＰＡＭ特異性を有する遺伝子操作ＣＲＩＳＰＲ−Ｃａｓ９ヌクレアーゼ |
| IL293323B2 (en) | 2015-06-18 | 2024-01-01 | Massachusetts Inst Technology | Crispr enzyme mutations reducing off-target effects |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| WO2017015015A1 (en) | 2015-07-17 | 2017-01-26 | Emory University | Crispr-associated protein from francisella and uses related thereto |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| AU2016316845B2 (en) | 2015-08-28 | 2022-03-10 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| CN108350453A (zh) | 2015-09-11 | 2018-07-31 | 通用医疗公司 | 核酸酶dsb的完全查询和测序(find-seq) |
| JP2018529353A (ja) | 2015-09-30 | 2018-10-11 | ザ ジェネラル ホスピタル コーポレイション | 配列決定法による切断事象の包括的生体外報告（ＣＩＲＣＬＥ−ｓｅｑ） |
| SG10202104041PA (en) * | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| EP3845668A1 (en) | 2015-10-30 | 2021-07-07 | New England Biolabs, Inc. | Compositions and methods for determining modified cytosines by sequencing |
| AU2016348439B2 (en) | 2015-11-04 | 2023-03-09 | Atreca, Inc. | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
| EP3374501B1 (en) | 2015-11-11 | 2023-07-12 | Lonza Ltd | Crispr-associated (cas) proteins with reduced immunogenicity |
| US9896696B2 (en) | 2016-02-15 | 2018-02-20 | Benson Hill Biosystems, Inc. | Compositions and methods for modifying genomes |
| CN105543195A (zh) * | 2016-03-01 | 2016-05-04 | 中国水稻研究所 | 植物Cas9变体蛋白VQR及其编码基因与应用 |
| SG11201810179RA (en) | 2016-04-19 | 2018-12-28 | Broad Inst Inc | Novel crispr enzymes and systems |
| CN109312386B (zh) * | 2016-06-15 | 2022-10-25 | 株式会社图尔金 | 使用中靶靶标和脱靶靶标的多重靶标系统筛选靶特异性核酸酶的方法及其用途 |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| WO2018052247A1 (ko) | 2016-09-13 | 2018-03-22 | 주식회사 툴젠 | 시토신 디아미나제에 의한 dna에서의 염기 교정 확인 방법 |
| CA3039409A1 (en) | 2016-10-07 | 2018-04-12 | Integrated Dna Technologies, Inc. | S. pyogenes cas9 mutant genes and polypeptides encoded by same |
| US11242542B2 (en) | 2016-10-07 | 2022-02-08 | Integrated Dna Technologies, Inc. | S. pyogenes Cas9 mutant genes and polypeptides encoded by same |
| SG10201913505WA (en) | 2016-10-17 | 2020-02-27 | Univ Nanyang Tech | Truncated crispr-cas proteins for dna targeting |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| AU2018273986A1 (en) | 2017-05-25 | 2019-12-12 | The General Hospital Corporation | Bipartite base editor (BBE) architectures and type-II-C-Cas9 zinc finger editing |
| CN110914423B (zh) * | 2017-05-31 | 2024-02-06 | 国立大学法人东京大学 | 经修饰的Cas9蛋白及其用途 |
| US20200140835A1 (en) | 2017-06-06 | 2020-05-07 | The General Hospital Corporation | Engineered CRISPR-Cas9 Nucleases |
| BR112020000310A2 (pt) | 2017-07-07 | 2020-07-14 | Toolgen Incorporated | variantes de crispr alvo específicas |
| CA3073448A1 (en) | 2017-08-23 | 2019-02-28 | The General Hospital Corporation | Engineered crispr-cas9 nucleases with altered pam specificity |
| WO2019075197A1 (en) | 2017-10-11 | 2019-04-18 | The General Hospital Corporation | METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES |
| EP3707252A1 (en) | 2017-11-10 | 2020-09-16 | Novozymes A/S | Temperature-sensitive cas9 protein |
| EP3781585A4 (en) | 2018-04-17 | 2022-01-26 | The General Hospital Corporation | SENSITIVE IN VITRO TESTS FOR SUBSTRATE PREFERENCES AND SITE FOR NUCLEIC ACID BINDERS, MODIFIERS AND CLEAVAGE AGENTS |
| KR20250133482A (ko) | 2018-05-11 | 2025-09-05 | 빔 테라퓨틱스, 인크. | 프로그래밍가능한 염기 편집기 시스템을 이용하여 단일 뉴클레오티드 다형성을 편집하는 방법 |
| US20230021641A1 (en) | 2018-08-23 | 2023-01-26 | The Broad Institute, Inc. | Cas9 variants having non-canonical pam specificities and uses thereof |
| US20220411777A1 (en) | 2019-08-30 | 2022-12-29 | The General Hospital Corporation | C-to-G Transversion DNA Base Editors |
| WO2021151073A2 (en) | 2020-01-24 | 2021-07-29 | The General Hospital Corporation | Unconstrained genome targeting with near-pamless engineered crispr-cas9 variants |
| WO2021151085A2 (en) | 2020-01-24 | 2021-07-29 | The General Hospital Corporation | Crispr-cas enzymes with enhanced on-target activity |
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