JP2020172536A - 創傷治癒のための幹細胞 - Google Patents
創傷治癒のための幹細胞 Download PDFInfo
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Abstract
Description
本発明は、本出願に記載される細胞を適用することによって創傷を処置するための方法を提供する。一態様では、前記方法は、皮膚創傷の処置を提供する。一般的な実施形態では、細胞は、送達ビヒクル中の官能化基材に結合されずに、創傷に送達される。
皮膚は、傷害および微生物からの身体防御の最前線であり、身体機能において重要な役割を果たす。外傷性傷害、火傷および慢性潰瘍は、皮膚障壁の主な免疫機能に影響を及ぼす重度の皮膚損傷を引き起こし、全身リスクを伴い得る。
本発明の実施形態の例として、以下の項目が挙げられる。
(項目1)
創傷治癒を促進するために十分な有効量および時間で細胞(I)を投与することによって、被験体における創傷治癒を促進する方法であって、該細胞(I)が官能化基材から送達されず、該細胞(I)が、oct4またはテロメラーゼを発現する非胚性非生殖細胞であり、形質転換されておらず、腫瘍原性ではなく、正常核型を有する、方法。
(項目2)
前記細胞(I)が、rex1、rox1またはsox2の1つまたはそれよりも多くをさらに発現する、項目1に記載の方法。
(項目3)
前記細胞(I)が、内胚葉性胚系統、外胚葉性胚系統および中胚葉性胚系統の少なくとも2つのうちの少なくとも1つの細胞型に分化し得る、項目1または2のいずれかに記載の方法。
(項目4)
前記細胞(I)が、内胚葉性胚系統、外胚葉性胚系統および中胚葉性胚系統のそれぞれの少なくとも1つの細胞型に分化し得る、項目3に記載の方法。
(項目5)
前記創傷が、皮膚および下層組織の創傷である、項目1に記載の方法。
(項目6)
前記創傷が潰瘍である、項目1または2のいずれかに記載の方法。
(項目7)
前記潰瘍が、足、手、下肢または腕に見られる皮膚潰瘍および静脈性下肢潰瘍からなる群より選択される、項目6に記載の方法。
(項目8)
前記皮膚潰瘍が、糖尿病および鎌状赤血球貧血からなる群より選択される疾患によって引き起こされる、項目7に記載の方法。
(項目9)
前記細胞(I)が遺伝子操作されていない、項目1または2のいずれかに記載の方法。
(項目10)
前記細胞(I)が骨髄に由来する、項目1または2のいずれかに記載の方法。
(項目11)
前記細胞(I)がヒトに由来する、項目1または2のいずれかに記載の方法。
(項目12)
前記被験体がヒトである、項目1または2のいずれかに記載の方法。
(項目13)
前記創傷が、末梢血またはリンパ系の不十分な循環によって引き起こされる、項目6に記載の方法。
(項目14)
前記細胞(I)を前記創傷に局所投与する、項目1または2のいずれかに記載の方法。
(項目15)
前記細胞(I)を皮下送達する、項目1または2のいずれかに記載の方法。
(項目16)
注射によって前記細胞(I)を前記創傷に投与する、項目1または2のいずれかに記載の方法。
(項目17)
液体細胞懸濁液で前記細胞(I)を投与する、項目1または2のいずれかに記載の方法。
(項目18)
リザーバーを使用して前記細胞(I)を前記創傷に投与する、項目1または2のいずれかに記載の方法。
(項目19)
前記細胞(I)が同種のものである、項目1または2のいずれかに記載の方法。
本発明は、本明細書に記載される特定の方法論、プロトコールおよび試薬などに限定されず、従って、変化し得ると理解すべきである。本明細書で使用される専門用語は、特定の実施形態を説明することのみを目的としたものであり、開示される本発明の範囲(これは、特許請求の範囲によってのみ規定される)を限定することを意図しない。
定義
MAPC
MAPCの単離および成長
米国特許第7,015,037号に記載されているヒト骨髄由来のMAPC
さらなる培養方法
医薬製剤
MAPCは、リンパ脈管形成潜在能およびリンパ脈管新生潜在能を有する
本発明者らは、MAPCが、LECを生じさせる固有能力を有するかを調査した。最初に、本発明者らは、VEGF−A曝露により、MAPCが一般的なECマーカーの発現を獲得することを確認した(図1A、B)。内皮分化の2週間(2w)でMAPCでは、Prox1(リンパ管分化のマスタースイッチ)が有意に誘導され、その発現レベルは、1週間後まで依然として安定していた(図1A、B)。Prox1誘導はまた、強制的なProx1発現によってアップレギュレートされることが公知のさらなるリンパ系遺伝子(すなわち、PdpnおよびItg9a)(36)の発現をトリガーした可能性がある。VEGF−Aに曝露した一定割合(21±6%)のMAPCはまた、LYVE1を発現した(mMAPCについては、タンパク質レベルで示されている;図1C)。特に、リンパ脈管新生GF VEGF−Cの存在下では、hMAPCにおけるリンパ系マーカー遺伝子発現の誘導はさらに改善されなかった(図1DにおいてLYVE1について示されている;VEGF−A、VEGF−Cまたは組み合わせに曝露すると、0日目に対するPROX1誘導倍率も同程度であった:それぞれ26±10、26±14および26±11;n=4)。COUP−TFII(ECのリンパ能力(lymphatic competence)を共同決定する転写因子)(36、37)は、分化過程を通して比較的高い一定レベルで発現していた(示さず)。したがって、MAPCは、LEC分化プログラムを開始させる固有能力を有する。
MAPCは、創傷治癒中の生理学的リンパ脈管新生に寄与する
MAPCは、二次リンパ浮腫モデルにおけるリンパ管を再生する
)がより頻繁に観察されたが(図8I)、皮膚リンパの大部分(67±5%)は依然として、αSMAコーティングを欠いていた。それにもかかわらず、hMAPC移植は、排出(プレ)集合管(collector)の数を有意に3倍増加させた(表1)。したがって、MAPCは、創傷境界を越えて既存のリンパネットワークのギャップを埋めることによって、二次リンパ浮腫におけるリンパ機能不全を回復させた。
MAPCは、移植リンパ節を宿主リンパネットワークに再接続する
方法
MAPCの誘導および分化
RNA単離、cDNA調製、qRT−PCRおよびフローサイトメトリー
インビトロLEC機能アッセイ
マウスモデル
組織学および形態計測
統計
拡張された方法
MAPCの誘導および分化
RNA単離、cDNA調製、qRT−PCRおよびフローサイトメトリー
インビトロLEC機能アッセイ
マウスモデル
組織学および形態計測
統計
表
Claims (1)
- 本願明細書に記載の発明。
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JP2022163207A (ja) | 2022-10-25 |
JP2019506396A (ja) | 2019-03-07 |
AU2022211794A1 (en) | 2022-08-25 |
US11918609B2 (en) | 2024-03-05 |
AU2016387339B2 (en) | 2022-11-17 |
US20220031756A1 (en) | 2022-02-03 |
SG10201914102UA (en) | 2020-03-30 |
HK1256413A1 (zh) | 2019-09-20 |
WO2017127123A1 (en) | 2017-07-27 |
CN108884437A (zh) | 2018-11-23 |
AU2016387339A1 (en) | 2018-08-23 |
KR20180102661A (ko) | 2018-09-17 |
EP3405566B1 (en) | 2023-10-18 |
EP3405566A1 (en) | 2018-11-28 |
US10967006B2 (en) | 2021-04-06 |
CA3012330A1 (en) | 2017-07-27 |
SG11201806245TA (en) | 2018-08-30 |
EP3405566A4 (en) | 2019-07-31 |
NZ745530A (en) | 2023-03-31 |
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