JP2020130181A5 - - Google Patents
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- Publication number
- JP2020130181A5 JP2020130181A5 JP2020024006A JP2020024006A JP2020130181A5 JP 2020130181 A5 JP2020130181 A5 JP 2020130181A5 JP 2020024006 A JP2020024006 A JP 2020024006A JP 2020024006 A JP2020024006 A JP 2020024006A JP 2020130181 A5 JP2020130181 A5 JP 2020130181A5
- Authority
- JP
- Japan
- Prior art keywords
- injection pipette
- injection
- tip
- pipette
- egg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007924 injection Substances 0.000 claims description 407
- 238000002347 injection Methods 0.000 claims description 403
- 235000013601 eggs Nutrition 0.000 claims description 161
- 239000000243 solution Substances 0.000 claims description 114
- 239000007788 liquid Substances 0.000 claims description 79
- 230000002093 peripheral Effects 0.000 claims description 59
- 239000011521 glass Substances 0.000 claims description 42
- 210000000170 Cell Membrane Anatomy 0.000 claims description 35
- 210000000805 Cytoplasm Anatomy 0.000 claims description 29
- 210000000681 Sperm Head Anatomy 0.000 claims description 16
- 210000004027 cells Anatomy 0.000 claims description 16
- 239000002480 mineral oil Substances 0.000 claims description 11
- 235000010446 mineral oil Nutrition 0.000 claims description 11
- 230000037250 Clearance Effects 0.000 claims description 10
- 241000124008 Mammalia Species 0.000 claims description 10
- 230000035512 clearance Effects 0.000 claims description 10
- 230000035939 shock Effects 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 4
- 238000001574 biopsy Methods 0.000 claims description 4
- 238000010449 nuclear transplantation Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 40
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 40
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 40
- 239000003921 oil Substances 0.000 description 37
- 238000005259 measurement Methods 0.000 description 31
- 230000029803 blastocyst development Effects 0.000 description 29
- 241000282898 Sus scrofa Species 0.000 description 23
- 210000004340 Zona Pellucida Anatomy 0.000 description 22
- 238000011156 evaluation Methods 0.000 description 19
- 210000002459 Blastocyst Anatomy 0.000 description 17
- 241000282887 Suidae Species 0.000 description 11
- 238000010586 diagram Methods 0.000 description 11
- 239000001963 growth media Substances 0.000 description 11
- 240000007742 Raphanus sativus Species 0.000 description 10
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 10
- 238000007796 conventional method Methods 0.000 description 10
- 239000012528 membrane Substances 0.000 description 10
- 230000004083 survival Effects 0.000 description 10
- 238000005452 bending Methods 0.000 description 7
- RVZRBWKZFJCCIB-UHFFFAOYSA-N Perfluorotributylamine Chemical compound FC(F)(F)C(F)(F)C(F)(F)C(F)(F)N(C(F)(F)C(F)(F)C(F)(F)C(F)(F)F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F RVZRBWKZFJCCIB-UHFFFAOYSA-N 0.000 description 6
- 238000005498 polishing Methods 0.000 description 6
- 210000004681 Ovum Anatomy 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000002257 embryonic structures Anatomy 0.000 description 5
- 238000000520 microinjection Methods 0.000 description 5
- 210000004508 polar body Anatomy 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229920000160 (ribonucleotides)n+m Polymers 0.000 description 3
- 210000001161 Embryo, Mammalian Anatomy 0.000 description 3
- 210000004940 Nucleus Anatomy 0.000 description 3
- 230000000875 corresponding Effects 0.000 description 3
- 229920003013 deoxyribonucleic acid Polymers 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000003204 osmotic Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 230000013080 embryo development ending in birth or egg hatching Effects 0.000 description 2
- 230000013144 embryo development ending in seed dormancy Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 210000004369 Blood Anatomy 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000030120 acrosome reaction Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000002068 genetic Effects 0.000 description 1
- 238000010362 genome editing Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002609 media Substances 0.000 description 1
- 230000003287 optical Effects 0.000 description 1
- 230000000149 penetrating Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000010374 somatic cell nuclear transfer Methods 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019027726 | 2019-02-19 | ||
| JP2019027726 | 2019-02-19 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020130181A JP2020130181A (ja) | 2020-08-31 |
| JP2020130181A5 true JP2020130181A5 (de) | 2020-11-12 |
| JP6870178B2 JP6870178B2 (ja) | 2021-05-12 |
Family
ID=72145002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020024006A Active JP6870178B2 (ja) | 2019-02-19 | 2020-02-17 | 卵細胞質内注入法 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP6870178B2 (de) |
| WO (1) | WO2020171038A1 (de) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112553057A (zh) * | 2020-12-11 | 2021-03-26 | 东蕴医疗科技(上海)有限公司 | 一种用于压电式单精子注射的显微操作针及其制作方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4664097A (en) * | 1984-05-09 | 1987-05-12 | The Wistar Institute Of Anatomy & Biology | Nuclear transplantation in the mammalian embryo by microsurgery and cell fusion |
| JPH0690770A (ja) * | 1991-03-29 | 1994-04-05 | Shimadzu Corp | マイクロマニピュレータ用微小器具 |
| JP2002204945A (ja) * | 2000-10-16 | 2002-07-23 | Ngk Insulators Ltd | マイクロピペット、分注装置及びバイオチップの製造方法 |
| JP2004325836A (ja) * | 2003-04-25 | 2004-11-18 | Suruga Seiki Kk | マイクロマニピュレータ |
| JP2005074586A (ja) * | 2003-09-01 | 2005-03-24 | Seoul National Univ Industry Foundation | 改選された卵細胞質内精子注入法（Ａｎｉｍｐｒｏｖｅｄｍｅｔｈｏｄｏｆｉｎｔｒａｃｙｔｏｐｌａｓｍｉｃｓｐｅｒｍｉｎｊｅｃｔｉｏｎ） |
| JP5051617B2 (ja) * | 2007-12-17 | 2012-10-17 | 富士電機株式会社 | 遠心分離方法及び遠心分離装置 |
| WO2018021103A1 (ja) * | 2016-07-26 | 2018-02-01 | コニカミノルタ株式会社 | ピペットチップ、送液方法および送液システム |
| JP6862624B2 (ja) * | 2018-03-13 | 2021-04-21 | プライムテック株式会社 | 卵細胞質内注入法 |
-
2020
- 2020-02-17 JP JP2020024006A patent/JP6870178B2/ja active Active
- 2020-02-18 WO PCT/JP2020/006168 patent/WO2020171038A1/ja active Application Filing
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