JP2020103683A - Needle puncture aid - Google Patents

Abstract

To provide a puncture aid that fixes a tail part in order to puncture a needle to an outside tail vein of the tail part of an experimental animal belonging to rodents.SOLUTION: A needle puncture aid is provided in which a tail fixing block with a V-shaped groove being exposed of both of a front end cross section and a rear edge cross section given on the surface is fixed on a base along with a syringe holding block for placing a syringe and having a slide groove capable of sliding the syringe so as to make a needle move along a V-shape opening of the V-shaped groove from the rear edge cross section given on the surface. The needle puncture aid is characterized in that the tail fixing block has a slope extending toward the base in the syringe holding block direction from the bottom vicinity of the V-shaped groove of the rear edge cross section, and the syringe holding block has a height adjustment mechanism for giving adjustment of a distance from the base of the slide groove and capable of adjusting a distance between the needle and the opening of the V-shaped groove.SELECTED DRAWING: Figure 1

Description

The present invention relates to an auxiliary device for puncturing an outer tail vein of a tail of an experimental animal belonging to a rodent eye with an injection needle, and in particular, fixing the tail of the experimental animal without requiring anesthesia to fix the outer tail. The present invention relates to an injection needle puncture assisting device for surely puncturing a vein with an injection needle.

BACKGROUND ART An injection needle is pierced into the tail of an experimental animal belonging to a rodent order such as a mouse or a rat to administer a drug or collect blood. Such a puncture is often performed by the operator's technique, and there have been proposed a retaining device for securing the experimental animal to ensure the puncture, and a fixing device for fixing the tail portion which is the puncture portion.

For example, in Patent Document 1, a hole for a hind limb that allows the hind limb of a mouse to pass to a knee is provided at a position separated by a predetermined distance on the left and right from a center line passing through the center of a plate-shaped body, and a mouse tail is inserted in the center. A retainer having a tail hole is disclosed. When the mouse is held on such a retainer and an injection needle is to be punctured in the tail, the mouse instinctively moves forward, which makes it difficult for the person in charge to bite the hand.

Further, in Patent Document 2, a retainer that retains and accommodates the body of the mouse between the recess of the lower mold block and the recess of the upper mold block, and extends and exposes the tail portion from the rear hole of the upper and lower mold blocks. Is disclosed. Further, Patent Document 3 discloses a fixture provided with a plurality of fixing members for sandwiching and fixing the puncture target site on the tail of the mouse in order to fix the puncture target site in a stable state.

By the way, there is a thin vein in the outer part of the tail of an experimental animal belonging to the rodent order, and an injection needle is pierced into the outer tail vein to administer a drug, or blood is collected. Since such a lateral tail vein is subcutaneous, skill is required for accurate puncture.

For example, Patent Document 4 discloses an injection needle with a guide that can easily and surely puncture the lateral caudal vein of a rat and collect blood without skill. That is, by providing a small-diameter fixing portion on the outer periphery of the proximal end portion of the blood collecting needle, the blood collecting needle formed by the line connecting the tip of the blood collecting needle and the outer peripheral surface of the small diameter fixing portion and the axial center line of the blood collecting needle. The authors say that the puncture angle can be made smaller and it will be easier to puncture by matching the tip of the blood collection needle to be punctured with the thin lateral tail vein of the rat. Here, the blood sampling needle is punctured from the tangential direction while slightly bending the tail of the rat.

JP, 2008-212410, A JP, 2011-153914, A JP, 2013-244095, A JP, 2006-223334, A

Avoid long guideline punctures or long-term anesthesia administration of anesthetic needles in experimental animals, which may cause unnecessary pain to experimental animals and should be avoided as a guideline. Should be On the other hand, in particular, the lateral tail vein of mice is very thin, about 0.3 mm, and its acquisition is not easy. Furthermore, the failure is not allowed in the case of tail vein injection of a small amount of expensive drug. Therefore, there is a need for an auxiliary device that can fix the tail of an experimental animal and puncture an injection needle with a high probability so that the tail vein can be injected reliably in a short time.

The present invention has been made in view of the above circumstances, and an object thereof is to provide a puncture assist for fixing a tail portion of a laboratory animal belonging to a rodent order in order to puncture the outer tail vein of the tail portion with an injection needle. To provide equipment.

An injection needle puncture assisting device according to the present invention is an assisting device for puncturing an injection needle into the lateral tail vein of the tail of an experimental animal belonging to rodent, and exposes both the front end section and the rear end section. A tail fixing block provided with the V-shaped groove on the surface thereof, and a slide on which the syringe is placed and the syringe can be slid so as to move the injection needle from the rear end section along the opening of the V-shaped groove. A syringe holding block provided with a groove on its surface is fixed on a base, and the tail fixing block is located in the direction of the syringe holding block from the vicinity of the bottom of the V-shaped groove of the rear end section. The syringe holding block has an inclined surface extending toward the base, and the syringe holding block can adjust the distance of the slide groove from the base to adjust the distance between the injection needle and the opening of the V-shaped groove. It is characterized by having a height adjusting mechanism that operates.

According to this invention, the tail portion can be fixed in order to puncture the lateral tail vein of the tail portion of the experimental animal belonging to the rodent order with the injection needle.

The invention described above may be characterized by having a temperature adjusting mechanism for heating the tail fixing block. According to such an invention, the distension of the outer tail vein can be promoted so as to facilitate puncturing of the outer tail vein of the tail portion of the experimental animal fixed to the fixing block with the injection needle.

In the above invention, the temperature adjusting mechanism may be characterized in that a heater is attached to the tail fixing block. Further, the tail fixing block may be provided with a temperature-sensitive material whose color changes with temperature on the surface of the tail fixing block. According to this invention, it is easy to heat the temperature of the tail of the experimental animal fixed to the fixing block and adjust its temperature.

In the above-mentioned invention, the inclined surface is a curved surface, and when the tail portion arranged along the V-shaped groove is fixed along the inclined surface, a tangent line of the tail portion that bends at the rear end section is formed. It may be characterized in that the injection needle can be moved along. According to this invention, the injection needle can be moved in the direction in which the lateral tail vein extends along the surface of the tail portion, and the puncture into the lateral tail vein can be facilitated.

In the invention described above, the syringe holding block is composed of two block bodies that have a slidable plane inclined with respect to the surface thereof and can be divided, and move along the slidable plane to parallelize the syringe. The distance of the slide groove from the base may be adjusted while maintaining the above value. According to this invention, the position of the injection needle with respect to the lateral tail vein can be easily adjusted, and the puncture of the injection needle into the lateral tail vein can be facilitated.

In the invention described above, the tail fixing block may include a front fixing portion that fixes the tail portion in front of the front end section cross section and allows the tail portion to be arranged along the V-shaped groove. Further, the front fixing part may be characterized in that it includes a clip for holding the tail part and providing hemostasis and fixing the tail part. According to this invention, it is possible to easily fix the tail portion, promote distension of the lateral tail vein, and easily puncture the lateral tail vein with an injection needle.

1 is a side view of an injection needle puncture assisting device according to an embodiment of the present invention. It is (a) side view and (b) front view of a tail fixed block. It is (a) side view and (b) front view of a syringe holding block. It is a side view which shows the usage example of the injection needle puncture assistance instrument. It is a side view of the principal part which shows the usage example of the injection needle puncture assistance device. It is a table|surface which shows the injection time by the presence or absence of use of the needle puncture assistance device.

Hereinafter, the injection needle puncture assisting device, which is one embodiment of the present invention, will be described in detail with reference to FIGS. 1 to 3.

As shown in FIG. 1, an injection needle puncture assisting device 100 includes a tail fixing block 10 fixed to the front on a substantially flat plate-like base 1, a syringe holding block 20 arranged rearward, and a tail fixing block 10. And a front fixing portion 5 arranged in front of. The injection needle puncture assisting device 100 fixes the tail 50 of an experimental animal belonging to a rodent order such as a rat or mouse to the tail fixing block 10, and causes the syringe holding block 20 to hold the syringe 30. The injection needle puncture assisting device 100 is an auxiliary device for puncturing the outer tail vein 51 of the fixed tail portion 50 with the injection needle 31 of the syringe 30.

In order to simplify the description, of the main surface of the base 1, the surface on which the tail fixing block 10 and the syringe holding block 20 are arranged is defined as the upper surface, and the top and bottom are defined, and the tail fixing block 10 side is the front, The side of the syringe holding block is the rear side, and the left and right are determined accordingly. However, since the injection needle puncture assisting device 100 may be used while being tilted, this vertical direction does not always coincide with the gravity direction.

Referring also to FIG. 2, the tail fixing block 10 comprises a V-shaped groove 11 extending from a front end cross section 15a of the upper surface 15 to a rear end cross section 15b and having an opening 11' on the upper side. That is, the V-shaped groove 11 is provided so as to be exposed on both the front end section 15a and the rear end section 15b. From the vicinity of the bottom of the V-shaped groove 11 of the rear end cross section 15b, an inclined surface 12 extending toward the rear base 1 is provided. The inclined surface 12 is a surface along which the tail portion 50 is fixed by applying tension toward the tip, and when the tail portion 50 is fixed along the inclined surface 12, the injection needle 31 is attached to the outer tail vein 51. The tail 50 is provided with a shape that facilitates puncturing at the rear end cross section 15b of the V-shaped groove 11 that is the starting point of the tangent line L. Here, an arcuate curved surface is formed in a side view with a straight line continuous from the bottom of the V-shaped groove 11 as a tangent line. For example, the tangent line L (see FIG. 5) of the tail portion 50 bent at the rear end cross section 15b of the V-shaped groove 11 is thereby also substantially parallel to the straight line from the bottom of the V-shaped groove 11. The inclined surface 12 is preferably a curved surface having a curvature equivalent to that of a side surface of a cylinder having a diameter of 15 mm so that the tail portion 50 has a curvature similar to that obtained by a technique of a skilled injection person.

The tail fixing block 10 further includes a foot portion 13 for mounting the tail fixing block 10 on the base 1, and a protrusion for mounting a rubber band 52 as one means for fixing the tail portion 50 in the V-shaped groove 11. 14 and. Since the foot portion 13 is flush with the tail fixing block 10, it also has a role of increasing the contact area with the heater 2 to enhance heat transfer. A sheet-type heater 2 is attached to the lower surface of the tail fixing block 10 as a temperature adjusting mechanism for heating the tail fixing block 10. Further, a temperature-sensitive liquid crystal sheet 3 is attached as a temperature-sensitive material to the side surface near the surface 15, and the temperature can be estimated by the change in the color. That is, the heating output of the heater 2 is adjusted based on the temperature estimated by the color change due to the temperature of the temperature-sensitive liquid crystal sheet 3, and the heating temperature of the V-shaped groove 11 is adjusted.

Referring also to FIG. 3, the syringe holding block 20 includes a base block body 21 arranged on the base 1, and a position adjustment block body 25 capable of adjusting the distance from the base 1 to the base block body 21. And two block bodies that can be divided from each other. The base block body 21 is pivotally attached at the front end of an arm portion 22 extending forward to the tail fixing block 10 via a pivot shaft 19 and is rotatable in a direction away from the base 1. That is, the base block body 21 can adjust the angle in the front-rear direction with respect to the tail fixing block 10.

Further, the base block body 21 slidably holds the position adjusting block body 25 between the left and right wall portions 23a and 23b, and on the sliding plane 24 inclined in the front-rear direction between them. The elongated hole 23c extending in parallel with the sliding plane 24 provided in the wall 23a accommodates the pin body 27 protruding from the position adjustment block body 25. This stabilizes the sliding direction of the position adjusting block body 25 and regulates the slidable distance. Further, in order to fix the slide position of the position adjustment block body 25, a screw 28 is threadably fitted into the wall portion 23a so that the tip can be pressed against the side surface of the position adjustment block body 25. It should be noted that the sliding plane 24 can be, for example, a plane that inclines upward at an angle of 20 degrees with respect to the bottom surface of the base block body 21.

That is, the position adjustment block body 25 can slide along the sliding plane 24 to adjust the height with respect to the base block body 21 according to the inclination of the sliding plane 24. That is, the height of the position adjustment block body 25 with respect to the base 1 can also be adjusted. Further, the position adjustment block body 25 is provided with a slide groove 26 on the upper surface thereof, which holds the syringe 30 slidably back and forth along the opening 11 ′ of the V-shaped groove 11. The slide groove 26 is preferably a groove having a curvature that matches the syringe barrel of the syringe 30, and may be a V-shaped groove so that it can slide while holding syringe barrels having different diameters. Thereby, the distance of the slide groove 26 from the base 1 can be adjusted, and the height of the injection needle 31 of the syringe 30 held in the slide groove 26 can be adjusted. Further, the distance including the height of the injection needle 31 with respect to the opening 11 ′ of the V-shaped groove 11 of the tail fixing block 10 fixed to the base 1 can be adjusted.

The front fixing part 5 fixes the tail part 50 so that it may be arrange|positioned along the V-shaped groove 11, for example, the support|pillar part 6 extended upwards from the base 1, and the clip 7 fixed to the upper end. Including. It is preferable that the clip 7 can fix the tail portion 50 as described above and that the tail portion 50 is pinched and pressed to stop the bleeding. The hemostasis may promote expansion of the lateral tail vein 51 and facilitate the puncture of the lateral tail vein 51 with the injection needle 31. The pressure holding the tail portion 50 of the clip 7 in this range is from 0.12 MPa to 0.18 MPa so that the tail portion 50 can be fixed while bleeding is stopped without causing unnecessary pain to the experimental animal. The optimum value is preferably about 0.16 MPa.

Next, a method of using the injection needle puncture assisting device 100 will be described with reference to FIGS. 1 and 2 in addition to FIGS. 4 and 5.

First, as shown in FIG. 4, the experimental animal 60 is put in the retainer 101, its posture is stabilized, and the tail portion 50 is projected rearward. The injection needle puncture assisting device 100 is installed on the inclined table 8 so that the base 1 is inclined about 30 degrees with respect to the horizontal plane, and the tail 50 is fixed to the tail fixing block 10. Tilting the base 1 by 30 degrees reproduces the injection posture of a skilled person who fixes the tail 50 coming out of the retainer 101 placed on the desk only with the forefinger and the thumb, and to the tail 50 of the injection needle 31. This is because it is easy to observe the invasion of.

Referring to FIG. 1 and FIG. 2 together, the tail portion 50 is fixed to the front fixing portion 5 by the clip 7 and also fixed to the rubber band 52, and is disposed inside the V-shaped groove 11 along the V-shaped groove 11. Further, it is preferable to attach a rubber band to the tip of the tail portion 50 and fix the tail portion 50 so that the tail portion 50 is tensioned downward. Alternatively, the tail portion 50 may be pulled from the hole (not shown) to the lower portion of the base 1. Alternatively, the tip of the tail portion 50 may be along the tail fixing block 10 with a band body similar to the rubber band 52. Then, the outer tail vein 51 is directed upward with respect to the base 1. As a result, the outer tail vein 51 is arranged above the center of the V-shaped groove 11, and the left and right positions of the injection needle 31 can be matched. Further, the fixation by the clip 7 stops the outer tail vein 51 of the tail portion 50 and urges angry.

Further, the tail fixing block 10 is heated by the heater 2. The output of the heater 2 is controlled by a transformer (not shown). The tail fixing block 10 is preferably heated in the range of 37°C to 45°C. Ethanol may be sprayed if the temperature is to be lowered quickly. The heating of the tail fixing block 10 also heats the fixed tail 50, further encouraging the expansion of the lateral tail vein 51. In order to perform such heating and temperature adjustment, the tail fixing block 10 is preferably made of a material having a relatively high thermal conductivity and an appropriate heat capacity, and for example, brass can be used.

For example, at room temperature of 25° C., when the tail fixing block 10 made of brass and having a weight of 135 g is heated using a silicon rubber heater MG 50×100 mm manufactured by As One Co., Ltd., the tail fixing block 10 is heated to 40° C. with an output of AC 50 V. It was possible to heat in 10 minutes. After that, if the output is set to about 30 V, a constant temperature of about 40° C. can be maintained. The temperature of the tail fixing block 10 may be manually adjusted while observing the color change of the temperature-sensitive liquid crystal sheet 3.

Next, the position of the syringe 30 is adjusted by the syringe holding block 20. In the syringe holding block 20, the height of the injection needle 31 of the syringe 30 is adjusted by sliding the position adjustment block body 25 along the sliding plane 24 to adjust the height with respect to the base block body 21. Since the position adjustment block body 25 moves in parallel, the height can be adjusted without changing the direction of the injection needle 31 of the syringe 30 held in the slide groove 26 on the upper surface thereof.

As shown in FIG. 5, the syringe 30 can be adjusted in height and its angle. That is, the syringe holding block 20 can be angled so as to separate the rear end of the syringe holding block 20 from the base 1 about the pivot shaft 19 of the arm portion 22. Along with this, the syringe 30 and its injection needle 31 are also given an angle.

The adjustment of the height and angle of the syringe 30 as described above is a tail portion on the rear end cross section 15b of the V-shaped groove 11 which is a starting point of bending of the tail portion 50 that is bent and fixed along the inclined surface 12. The position of the tip of the injection needle 31 is adjusted so that the surface of the injection needle 31 is placed on the surface of the injection needle 31, and at the insertion position, the injection needle 31 is set at a predetermined angle with respect to the surface of the tail portion 50. Done to give. It is preferable that the predetermined angle be equal to the injection posture by the skill of a skilled person. For example, it is a position and an angle at which the injection needle 31 can be pierced along the tangent line L of the bent tail 50 into the outer tail vein 51 at a depth of 0.1 to 0.3 mm under the skin of the tail 50. For example, the injection needle 31 with the name “29G” is pressed to such an extent that the surface of the tail portion 50 is slightly recessed, bends slightly upward, and faces the direction along the tangent line L.

After the puncturing position is determined in this manner, the syringe 30 is slid forward along the slide groove 26, the injection needle 31 is punctured into the tail 50 from the puncturing position, and the lateral tail vein 51 is punctured. You can

According to the injection needle puncture assisting device 100 as described above, the tail portion 50 can be fixed to puncture the injection needle 31 into the outer tail vein 51 of the tail portion 50 of the experimental animal 60 belonging to the rodent order. With the help of, the possibility that even an unskilled person can puncture the lateral tail vein 51 with the injection needle 31 can be improved.

For example, as shown in FIG. 6, the outside tail of the tail of a C57BL/6J black mouse (male, 10 weeks old) is compared between the case where an unskilled person does not use the needle stick assistance device 100 and the case where the device is used. The time taken for intravenous injection was measured. As a result, the working time required for the injection was shortened by using the needle puncture assist device 100, and the variation in the time for each time was also reduced.

Although the embodiment of the present invention and the modification based on the embodiment have been described above, the present invention is not necessarily limited to this, and a person skilled in the art deviates from the gist of the present invention or the scope of the appended claims. Without doing so, various alternatives and modifications could be found.

1 Base 5 Front Fixing Part 10 Tail Fixing Block 20 Syringe Holding Block 30 Syringe 100 Injection Needle Puncture Assist Device

Claims (8)

An auxiliary device for puncturing a lateral tail vein of an experimental animal belonging to Rodentia with an injection needle,
A tail fixing block provided with a V-shaped groove on its surface exposing both a front end section and a rear end section, and a syringe on which a syringe is placed and the injection needle is placed along the opening of the V-shaped groove to the rear end. A syringe holding block provided with a slide groove on the surface for sliding the syringe so that the syringe can be moved from the cross section, is fixed on a base,
The tail fixing block has an inclined surface extending from the vicinity of the bottom of the V-shaped groove of the rear end section toward the base in the direction of the syringe holding block, and the syringe holding block has the base of the slide groove. An injection needle puncture assisting device, which has a height adjusting mechanism that can adjust the distance from the table to adjust the distance between the injection needle and the opening of the V-shaped groove. The needle puncture assisting device according to claim 1, further comprising a temperature adjusting mechanism for heating the tail fixing block. 3. The needle puncture assisting device according to claim 2, wherein the temperature adjusting mechanism has a heater attached to the tail fixing block. The needle puncture assisting device according to claim 2 or 3, wherein a temperature sensitive material that changes color depending on temperature is provided on the surface of the tail fixing block. The inclined surface is a curved surface, and the injection needle is along a tangent line of the tail portion that bends at the rear end cross section when the tail portion arranged along the V-shaped groove is fixed along the inclined surface. The needle puncture assisting device according to any one of claims 1 to 4, characterized in that it can be moved. The syringe holding block is composed of two separable block bodies each having a slidable plane inclined with respect to the surface thereof and moving along the slidable plane to maintain the syringe in parallel with each other. The needle puncture aid according to any one of claims 1 to 5, wherein the slide groove is adjusted in distance from the base. 7. A front fixing part for fixing the tail part in front of the front end cross section of the tail part fixing block and for arranging the tail part along the V-shaped groove, according to one of claims 1 to 6. A needle puncture assisting device according to item 3. The needle puncture assisting device according to claim 7, wherein the front fixing portion includes a clip that holds the tail portion and holds the tail portion for hemostasis and fixing the tail portion.

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