WO2016101226A1 - Embryo transplantation device and operation method thereof - Google Patents
Embryo transplantation device and operation method thereof Download PDFInfo
- Publication number
- WO2016101226A1 WO2016101226A1 PCT/CN2014/095014 CN2014095014W WO2016101226A1 WO 2016101226 A1 WO2016101226 A1 WO 2016101226A1 CN 2014095014 W CN2014095014 W CN 2014095014W WO 2016101226 A1 WO2016101226 A1 WO 2016101226A1
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- inner core
- embryo
- joint
- injection device
- uterus
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
Definitions
- the present invention relates to the field of bioengineering, and in particular to an embryo transfer device and a method of operating the same. Background technique
- Embryo transfer is the biotechnology of transplanting mammalian early embryos produced in vitro and in vivo into the reproductive tract of the same female physiological state, and continuing to develop into normal individuals. It is genetics, development, reproductive biology, and An important technology in the field of animal breeding and breeding is an important part of the breeding of IVF, genetically modified animals, cloned animals and livestock.
- the individual providing the embryo is called the donor, and the individual receiving the embryo is called the recipient.
- Embryo transfer technology mainly involves mice, humans, cows, sheep, rabbits, horses, pigs, dogs, cats, rats, monkeys, deer, camels, etc., especially the former four species.
- a blastocyst (a specific stage of early embryonic development) is transplanted into the uterus of the recipient to continue its development
- the Chinese patent application No. CN201210379983.5 discloses an embryo transfer device and a method for using the same, which can accurately place an embryo into a correct position in the uterus under ultrasound guidance, thereby improving the success rate of embryo transfer.
- the embryo transfer device comprises a rigid support core and a through-transplant tube and a guide tube. The key point is that the front end of the guide tube is provided with an echogenic steel ring.
- the method of using the embryo transfer device includes the following steps: A. placing the support core into the guide tube, then inserting the guide tube into the uterus, observing the position of the echo rim of the guide tube by using an ultrasonic device, and adjusting the guide tube until the guide tube is at The appropriate position of the transplanted embryo, exit the support core; B.
- Non-surgical embryo transfer requires no surgery, is quick and easy, has fast receptor recovery, is less susceptible to infection, and has low cost of transplanting.
- mice and sheep are mainly transplanted by surgery because of their small size and physiological structure, while humans and cattle are mainly transplanted by non-surgical methods.
- the success rate of transplanting in mice is about 80% (the number of births accounts for the percentage of transplanted blastocysts); the success rate of sheep surgical transplantation is about 65%; the success rate of human non-surgical transplantation Less than 30%; the success rate of non-surgical transplantation of cattle is about 50%; the success rate of non-surgical transplantation in mice is about 30%.
- embryo transfer media In the field of embryo transfer research, a lot of research work has focused on embryo culture media, embryo implantation (or planting), embryo development, hormone regulation, etc., and there are few studies on embryo transfer devices and transplantation methods. We found: the damage caused by the transplanter to the endometrium of the recipient during embryo transfer, the volume of the transplant medium, the volume of the transplanted air, the location of the embryo to the uterus, the shape and material of the transplanter, and the operation of the transplanter. Methods and the like all significantly affect the success rate of embryo transfer.
- mice non-surgical embryo transfer devices have greater damage to the endometrium, more transplanted fluids, and improper operation methods, resulting in easy embryo loss and early embryo implantation death.
- the transplant success rate is only about 30%.
- Human and large animals The main disadvantages of non-surgical embryo transfer technology are that the transplanter is thicker and the liquid and air injected into the uterus are more. Improper transplantation methods result in the embryos easily overflowing the uterus or the implantation position is not ideal, reducing the success rate of transplantation.
- the transplanting device used for sheep embryo transfer is relatively thick, and the liquid and air are injected more, and the damage to the uterus is large and the embryo easily overflows the uterus. Summary of the invention
- the technical problem to be solved by the present invention is how to overcome the damage of the endometrium caused by the existing embryo transfer, the embryo easily overflowing the uterus and the improper implantation position of the embryo, resulting in the defect of the success rate of the transplantation.
- the present invention provides an embryo transfer device comprising a joint, a sealing tube and an inner core, the joint having a hollow tapered structure and having a thin end and a thick end, the thick end of the joint being used for An injection device is connected, one end of the sealing tube is connected to the thin end of the joint, and the other end of the sealing tube is connected to one end of the inner core, the inner core is a hollow tubular structure and has a curvature, and the inner core has a flat end Smooth, the inner core is smooth.
- the inner core is a circular arc having an arc of 20°-40°, the inner core has a length of 2-2.45 cm, and the inner core has an inner diameter of 0.31-0.49 mm, and the inner core is outside.
- the diameter is 0.61-0.79 mm.
- the inner core is a plastic material or a glass material.
- the invention also provides an embryo transfer device, comprising a protection tube, an outer sleeve, a joint and an inner core, wherein the protection tube is swelled at one end and used for being placed in a uterus or a uterine horn, and the outer sleeve is covered
- An outer surface of the core, the joint having a hollow tapered structure and having a thin end and a thick end, the thick end of the joint being for connection with an injection device, the thin end of the joint being connected to one end of the inner core, the inner
- the core is a hollow tubular structure and the inner wall is smooth, and the other end of the inner core is blunt and smooth.
- the inner core has a length of 20-70 cm, the inner core has an inner diameter of 0.2-0.4 mm, and the inner core has an outer diameter of 0.5-0.7 mm.
- the inner core is a plastic material or a glass material
- the outer sleeve and the protection tube are stainless steel materials or plastic materials.
- the invention also provides an embryo transfer device comprising a joint, a sealing tube and an inner core, the joint has a hollow tapered structure and has a thin end and a thick end, and the thick end of the joint is used for connecting with an injection device, One end of the sealing tube is connected to the thin end of the joint, the other end of the sealing tube is connected to one end of the inner core, the inner core is a hollow tubular structure, and the inner core has an inner diameter of 0.3-0.5 mm. The outer diameter of the core is 0.7-0.8 mm.
- the inner core is a plastic material or a glass material, and the inner core of the inner core is smooth and the top end is pure.
- the present invention also provides a method of operating an embryo transfer device, the method comprising the steps of:
- the inner core is sent into the cervix, and after the transplanter enters the cervix 8-10 mm, the injection device is rotated 90 degrees clockwise, and the rear end is pressed 30 degrees, and the transplanter slowly enters the uterus;
- the embryo transfer recipient level is allowed to stand for 2-3 min;
- the present invention provides a method of operating an embryo transfer device, the method comprising the steps of:
- the inner core is sent to the protection tube.
- the inner core is fixed, and the protection tube is retracted, so that the inner core is placed at a proper position in the uterus;
- the inner core of the embryo transfer device of the invention has a certain curvature, the inner wall is smooth, the embryo is easily sucked, and the material is soft, and the damage of the transplanter to the uterus can be minimized;
- the blunt garden is smooth, and the damage to the uterus is also reduced;
- the inner core has a certain length, and the embryo can be sent to a better position;
- the inner diameter is small, and the volume of the transplant solution can be reduced;
- the volume can be precisely controlled, the volume of the transplanted liquid is minimized, the embryo is properly implanted in the uterus, the embryo is prevented from overflowing the uterus, and the efficiency of embryo implantation is improved.
- the top end of the protective tube is swollen and blunt, and the inner core is left in the proper position of the uterus by the back protection tube to minimize the damage of the uterus; Smoothing the blunt body, also reducing the damage to the uterus; the inner core of the inner core is smooth, and the embryo can be easily sucked; the volume of the transplanter can be precisely controlled, the volume of the transplanted liquid is minimized, and the embryo implantation is improved. effectiveness.
- the transplanter of the present invention can seal the inner core and the joint by using a sealing tube, and autoclave When the sealing tube is more secure.
- the embryo transfer device of the present invention slowly rotates out of the uterus and stays, thereby maximally preventing the embryo from overflowing the uterus and improving the efficiency of embryo implantation.
- the protective tube and the inner core of the embryo transfer device of the present invention slowly rotate out of the uterus and stay, so as to prevent the embryo from overflowing the uterus and improve the efficiency of embryo implantation.
- the invention may also be used to inject a drug or liquid into the uterus for research or treatment purposes.
- FIG. 1 is a schematic view showing the structure of a mouse used in an embryo transfer device according to an embodiment of the present invention
- FIG. 2 is a schematic structural view showing an open tube in an embryo transfer device according to an embodiment of the present invention
- FIG. 3 is a schematic view showing the structure of an embryo transfer device for sheep according to an embodiment of the present invention.
- FIG. 4 is a schematic view showing the structure of an embryo transfer device for humans and large animals according to an embodiment of the present invention.
- Embodiment 1 is a diagrammatic representation of Embodiment 1:
- the present invention provides an embryo transfer device for use in a rat, including a joint 1, a sealing tube 2, and an inner core 3, which has a hollow tapered structure and has a fine The end and the thick end, the thick end of the joint 1 is connected to the injection device, one end of the sealing tube 2 is connected to the thin end of the joint 1, and the other end is connected to one end of the inner core 3, and the inner core 3 is Hollow tubular knot It has a certain curvature.
- the inner core 3 is a circular arc having an arc of 20°, the inner core 3 has a length of 2 cm, the inner core 3 has an inner diameter of 0.31 mm, and the inner core 3 has an outer diameter of 0.61 mm.
- the material of the inner core 3 is plastic, and its top end is blunt and smooth.
- the present invention also provides an embryo transfer device for use in humans or large animals such as cattle and horses, including a protective tube 6, an outer sleeve 5, a joint 1 and an inner core 3, said protection
- the tube 6 is expanded at one end to be placed in the uterus or the uterine horn.
- the outer sleeve 5 is covered on the outer surface of the inner core 3.
- the joint 1 has a hollow tapered structure and has a thin end and a thick end.
- the thick end of the joint 1 is for connection with an injection device, the thin end of which is connected to one end of the inner core 3, the inner core 3 is a hollow tubular structure, and the other end of the inner core 3 is blunt and smooth.
- the inner core 3 When the embryo transfer device is used in a human, the inner core 3 has a length of 22 cm, the inner core 3 has an inner diameter of 0.2 mm, and the inner core 3 has an outer diameter of 0.5 mm; the inner core 3 The top end is smoothed by simmering, the inner core 3 is made of plastic, and the outer sleeve 5 and the protective tube 6 are made of plastic.
- the inner core 3 When the embryo transfer device is used in a large animal such as a cow or a horse, the inner core 3 has a length of 40 cm, the inner core 3 has an inner diameter of 0.3 mm, and the inner core 3 has an outer diameter of 0.6 mm.
- the top end of the inner core 3 is made smooth by boring, the inner core 3 is made of plastic, and the outer sleeve 5 and the protective tube 6 are made of stainless steel.
- the protective tube has a length of 45 cm and a diameter of 3 mm.
- the outer tube has a length of 55 cm and a diameter of 1 mm.
- the present invention also provides an embryo transfer device for use in an embodiment of a sheep comprising a joint 1, a sealing tube 2 and an inner core 3, the joint 1 having a hollow tapered structure and having a thin end and a thick end, the thick end of the joint 1 is for connection with an injection device, one end of the sealing tube 2 is connected to the thin end of the joint 1, and the other end is connected to one end of the inner core 3, the inner core 3
- the inner core 3 In the hollow tubular structure, the inner core 3 has an inner diameter of 0.3 mm and the inner core 3 has an outer diameter of 0.7 mm.
- the joint 1 is connected to an injection device, and the inner core 3 is made of plastic and has a top end ⁇ Bake into a blunt round shape.
- the present invention also provides a method of operating an embryo transfer device for use in a mouse, the method comprising the steps of:
- the inner core 3 is sent into the cervix, and after the transplanter enters the cervix 8 mm, the injection device rotates 90 degrees clockwise, and the rear end is pressed 30 degrees, and the transplanter slowly enters the uterus;
- the embryo transfer recipient level is allowed to stand for 2-3 min;
- the present invention also provides a method of operating an embryo transfer device for use in humans and large animals, the method comprising the steps of:
- the embryo is sucked to the top of the inner core 3 covered with the outer sleeve 5;
- the protective tube 6 is placed in the human or animal uterus through the cervix;
- the inner core 3 is sent to the protection tube 6.
- the inner core 3 is fixed, and the protection tube 6 is retracted, so that the inner core 3 is placed at the proper position of the uterus;
- Embodiment 2 is a diagrammatic representation of Embodiment 1:
- the present invention provides an embryo transfer device for use in a rat, including a joint 1, a sealing tube 2, and an inner core 3, which has a hollow tapered structure and has a fine The end and the thick end, the thick end of the joint 1 is connected to the injection device, one end of the sealing tube 2 is connected to the thin end of the joint 1, and the other end is connected to one end of the inner core 3, and the inner core 3 is Hollow tubular structure with a certain degree of curvature.
- the inner core 3 is a circular arc having an arc of 30°, the inner core 3 has a length of 2.45 cm, the inner core 3 has an inner diameter of 0.49 mm, and the inner core 3 has an outer diameter of 0.79 mm.
- the material of the inner core 3 is glass, and its top end is blunt and smooth.
- the present invention also provides an embryo transfer device for use in humans or large animals such as cattle and horses, including a protective tube 6, an outer sleeve 5, a joint 1 and an inner core 3, said protection
- the tube 6 is expanded at one end to be placed in the uterus or the uterine horn.
- the outer sleeve 5 is covered on the outer surface of the inner core 3.
- the joint 1 has a hollow tapered structure and has a thin end and a thick end.
- the thick end of the joint 1 is for connection with an injection device, the thin end of which is connected to one end of the inner core 3, the inner core 3 is a hollow tubular structure, and the other end of the inner core 3 is blunt and smooth.
- the inner core 3 When the embryo transfer device is used in a human, the inner core 3 has a length of 30 cm, the inner core 3 has an inner diameter of 0.25 mm, and the inner core 3 has an outer diameter of 0.55 mm; the inner core 3 The top end is smoothed by simmering, the inner core 3 is made of plastic, and the outer sleeve 5 and the protective tube 6 are made of plastic.
- the inner core 3 When the embryo transfer device is used in a large animal such as a cow or a horse, the inner core 3 has a length of 55 cm, the inner core 3 has an inner diameter of 0.35 mm, and the inner core 3 has an outer diameter of 0.7 mm.
- the top end of the inner core 3 is made smooth by simmering, the inner core 3 is made of plastic, and the outer sleeve 5 and the protective tube 6 are made of stainless steel.
- the protective tube has a length of 50 cm and a diameter of 3.5 mm.
- the outer sleeve is 60 cm in length and 1.2 mm in diameter.
- the present invention also provides an embryo transfer device for use in an embodiment of a sheep comprising a joint 1, a sealing tube 2 and an inner core 3, the joint 1 having a hollow tapered structure and having a thin end and a thick end, the thick end of the joint 1 is for connection with an injection device, one end of the sealing tube 2 is connected to the thin end of the joint 1, and the other end is connected to one end of the inner core 3, the inner core 3
- the inner core 3 In the hollow tubular structure, the inner core 3 has an inner diameter of 0.5 mm and the inner core 3 has an outer diameter of 0.8 mm.
- the inner core 3 is made of glass and its top end is baked into a blunt circular shape.
- the present invention also provides a method of operating an embryo transfer device for use in a mouse, the method comprising the steps of:
- the sputum tube 4 is sent to the rat vagina;
- the inner core 3 is sent into the cervix, and after the transplanter enters the cervix 9 mm, the injection device rotates 90 degrees clockwise, and the rear end is pressed 30 degrees, and the transplanter slowly enters the uterus;
- the embryo transfer recipient level is allowed to stand for 2-3 min;
- the present invention also provides a method of operating an embryo transfer device for use in humans and large animals, the method comprising the steps of:
- the protective tube 6 is placed in the human or animal uterus through the cervix;
- the inner core 3 is sent to the protection tube 6.
- the inner core 3 is fixed, and the protection tube 6 is retracted, so that the inner core 3 is placed at the proper position of the uterus;
- Embodiment 3 is a diagrammatic representation of Embodiment 3
- the present invention provides an embryo transfer device for use in a rat, including a joint 1, a sealing tube 2, and an inner core 3, which has a hollow tapered structure and has a fine The end and the thick end, the thick end of the joint 1 is connected to the injection device, one end of the sealing tube 2 is connected to the thin end of the joint 1, and the other end is connected to one end of the inner core 3, and the inner core 3 is Hollow tubular structure with a certain degree of curvature.
- the inner core 3 is a circular arc having an arc of 40°, the inner core 3 has a length of 2.2 cm, the inner core 3 has an inner diameter of 0.4 mm, and the inner core 3 has an outer diameter of 0.7 mm.
- the material glass of the inner core 3 has a blunt top end that is smooth.
- the present invention also provides an embryo transfer device for use in large animals such as cattle, horses, donkeys, camels, red deer, etc., including a protective tube 6, an outer sleeve 5, a joint 1 and an inner core.
- the outer sleeve 5 is coated on the outer surface of the inner core 3
- the joint 1 is hollow tapered structure and has a thin End and thick end, said connection The thick end of the head 1 is for connection with an injection device, the thin end of which is connected to one end of the inner core 3
- the inner core 3 is a hollow tubular structure, and the other end of the inner core 3 is blunt and smooth.
- the inner core 3 has a length of 65 cm, the inner core 3 has an inner diameter of 0.4 mm, and the inner core 3 has an outer diameter of 0.7 mm.
- the top end of the inner core 3 is made smooth by simmering, the material of the inner core 3 is plastic, and the outer sleeve 5 and the protective tube 6 are made of stainless steel.
- the protective tube has a length of 55 cm and a diameter of 4.0 mm.
- the outer sleeve is 65 cm in length and 1.0 mm in diameter.
- the present invention also provides an embryo transfer device for use in an embodiment of a sheep comprising a joint 1, a sealing tube 2 and an inner core 3, the joint 1 having a hollow tapered structure and having a thin end and a thick end, the thick end of the joint 1 is for connection with an injection device, one end of the sealing tube 2 is connected to the thin end of the joint 1, and the other end is connected to one end of the inner core 3, the inner core 3
- the inner core 3 has an inner diameter of 0.4 mm, and the inner core 3 has an outer diameter of 0.6 mm.
- the inner core 3 is made of glass and its top end is baked into a blunt circle. .
- the present invention also provides a method of operating an embryo transfer device for use in a mouse, the method comprising the steps of:
- the sputum tube 4 is sent to the rat vagina;
- the inner core 3 is sent into the cervix, and after the transplanter enters the cervix 10 mm, the injection device is rotated 90 degrees clockwise, and the rear end is pressed 30 degrees, and the transplanter slowly enters the uterus;
- the embryo transfer recipient level is allowed to stand for 2-3 min;
- the present invention also provides a method of operating an embryo transfer device for use in humans and large animals, the method comprising the steps of:
- the protective tube 6 is placed in the human or animal uterus through the cervix;
- the inner core 3 is sent to the protection tube 6.
- the inner core 3 is fixed, and the protection tube 6 is retracted, so that the inner core 3 is placed at the proper position of the uterus;
- the mammalian embryo transfer device of the present invention and the method for the same have the following beneficial effects: 1.
- the inner core of the mouse embryo transfer device of the invention has a certain curvature, the inner wall is smooth, the top end is blunt and the material is soft, and the maximum is Reducing damage to the uterus by the transplanter;
- the inner core has a certain length to deliver the embryo to a better position;
- the inner diameter is small, and the volume of the transplant solution can be reduced;
- the volume of the transplanter can be precisely controlled Minimize the volume of the transplanted liquid, maximize the embryo's correct position in the uterus, prevent the embryo from overflowing the uterus, and improve the efficiency of embryo implantation. 2.
- the top end of the protective tube is swollen and blunt, and the inner core is left in the proper position of the uterus by the back protection tube to minimize the damage of the uterus;
- the top is smoothed by simmering, which also reduces the damage to the uterus;
- the volume of the transplanter can be precisely controlled, the volume of the transplanted liquid is minimized, and the embryo is properly implanted in the uterus. Prevent embryos from overflowing the uterus and improve embryo implantation efficiency.
- the sheep embryo transfer device of the present invention can seal the inner core and the joint by using a sealing tube, and the sealing tube is more firm when autoclaved; the inner diameter of the inner core is small, and the volume of the transplanting liquid can be reduced; The top is smoothed by simmering to reduce the damage to the uterus; the volume of the transplanter can be precisely controlled, the volume of the transplanted liquid is minimized, and the embryo is properly implanted in the uterus to prevent it from being implanted. The embryo overflows the uterus and improves the efficiency of embryo implantation.
- the inner core of the murine embryo transfer device of the present invention slowly rotates out of the uterus and stays, thereby maximally preventing the embryo from overflowing the uterus.
- the human and large animal embryo transfer device protection tube and inner core of the present invention slowly rotate out of the uterus and stay, so as to prevent the embryo from overflowing the uterus to the utmost extent.
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PCT/CN2014/095014 WO2016101226A1 (en) | 2014-12-25 | 2014-12-25 | Embryo transplantation device and operation method thereof |
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PCT/CN2014/095014 WO2016101226A1 (en) | 2014-12-25 | 2014-12-25 | Embryo transplantation device and operation method thereof |
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Cited By (4)
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---|---|---|---|---|
CN109223143A (en) * | 2018-11-09 | 2019-01-18 | 潍坊护理职业学院 | A kind of embryo transplanter for test-tube baby |
CN110013292A (en) * | 2019-05-21 | 2019-07-16 | 安徽农业大学 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
CN110063776A (en) * | 2018-01-23 | 2019-07-30 | 河北农业大学 | A kind of human embryos grafting device |
CN110257229A (en) * | 2019-07-08 | 2019-09-20 | 河南省农业科学院畜牧兽医研究所 | It is a kind of simply to inhale fetus device and preparation method thereof |
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CN201227315Y (en) * | 2008-07-30 | 2009-04-29 | 毕宜法 | Embryo transplantation hose |
CN203017052U (en) * | 2012-12-27 | 2013-06-26 | 广州品致医疗器械有限公司 | Embryo transfer tube casing |
CN203089450U (en) * | 2012-12-21 | 2013-07-31 | 河北农业大学 | Non-surgical mice embryo transplantation device |
CN203898495U (en) * | 2014-03-31 | 2014-10-29 | 中国医学科学院医学生物学研究所 | Embryo transfer device for rat and mouse embryo transfer |
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FR2647668A1 (en) * | 1989-06-06 | 1990-12-07 | Medizin Labortechnik Veb K | Transfer pipette for embryos |
CN2360049Y (en) * | 1998-05-08 | 2000-01-26 | 上海市儿童医院上海医学遗传研究所 | Sheep multicelluter embryo tramsplanter |
EP1721579A1 (en) * | 2005-05-10 | 2006-11-15 | Hans-Peter Steiner | Embryo transfer catheter |
CN201227315Y (en) * | 2008-07-30 | 2009-04-29 | 毕宜法 | Embryo transplantation hose |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110063776A (en) * | 2018-01-23 | 2019-07-30 | 河北农业大学 | A kind of human embryos grafting device |
CN109223143A (en) * | 2018-11-09 | 2019-01-18 | 潍坊护理职业学院 | A kind of embryo transplanter for test-tube baby |
CN110013292A (en) * | 2019-05-21 | 2019-07-16 | 安徽农业大学 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
CN110257229A (en) * | 2019-07-08 | 2019-09-20 | 河南省农业科学院畜牧兽医研究所 | It is a kind of simply to inhale fetus device and preparation method thereof |
CN110257229B (en) * | 2019-07-08 | 2023-11-07 | 河南省农业科学院畜牧兽医研究所 | Simple embryo suction device and manufacturing method thereof |
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