CN110013292A - A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure - Google Patents
A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
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Abstract
The invention discloses a kind of shifting embryo needles for being able to maintain that blastaea stereochemical structure.The shifting embryo needle includes thin open end and thick open end, and the opening diameter of thick open end is greater than the opening diameter of thin open end；The interior circular diameter of thin open end is 0.20mm~0.28mm, and wall thickness is 0.01mm~0.05mm, and the port of thin open end is concordantly smooth.The thick open end for moving embryo needle is connect with one end of mouth suction pipe, the other end of mouth suction pipe is connect with pipetting tip, and thus assembling constitutes a kind of mouthful of suction and moves embryo device.The ozzle of the thick open end for moving embryo needle and syringe is connected by connecting tube, then constitutes a kind of hand-held shifting embryo device.The present invention provides a kind of specific purpose tools for collecting blastaea and can maintain blastaea stereochemical structure --- move embryo needle.The shifting embryo needle system is made of the common test material being easy to get, and production method is simple and convenient, easy grasp easy to operate.The working efficiency of blastaea collection can be greatly improved using the shifting embryo needle.
Description
Technical field
The present invention relates to a kind of shifting embryo needles and preparation method thereof for being able to maintain that blastaea stereochemical structure.
Background technique
Recently as flourishing for Embryo engineering technology, embryo transfer technology also increasingly tends to be mature, cures in reproduction
Learn aspect, more and more infertile patients can select to carry out " test-tube baby ", i.e., sperm and ovum are carried out in vitro by
Essence, which develops into embryo and moves into maternal uterine again, develops into fetus.Under normal conditions, development can be selected to cleavage stage or blastula stage
Embryo transplant.With the clinical experience of Embryo Culture system improved and save bit by bit for many years, people are more likely to selection capsule
Embryo carries out embryo transfer.Blastaea is further developed on the basis of cleavage stage embryo first, this process just to embryo into
Go again less preferred, the embryo that is more convenient for is in parent implantation；Furthermore blastaea has stronger vigor and adaptability than cleavage stage embryo,
It only needs to transplant 1~2 piece of blastaea during embryo transfer, reduces the incidence of multifetation；Importantly, normal
Embryo's spilting of an egg is occurred in fallopian tubal under the physiological status of internal fertilization, and development just enters uterus to blastaea period and carries out
Thus bed selects blastaea transplanting that embryo nidation cracking can be made to decrease embryo in the chance of fallopian tubal migration, reduces
The incidence of ectopic pregnancy.Since the ability that blastaea repairs freezing injury is strong, the influence to embryonic development potential is small, so blastaea
Culture occupies more and more important position during Embryo Culture system and embryo transfer.In terms of Animal husbandry production, people
For breeding and conservation, also can usually to domestic animal carry out In vitro fertilization-embryo transplantation, although the embryonic development of different animals
Process can be variant, but is also more blastaea to be selected to be transplanted during embryo transfer, since test-tube baby is
It is developed on the basis of having studied the physiological mechanism of various animals, so the blastocyst culture of animal is even more to receive people's
Favor.
The formation of blastaea morphologically experienced the expansion of cell division, cell densification, the appearance of blastocoele and blastocoele
The process of opening, gene regulation experienced from maternal factor and adjust the conversion adjusted to embryonic gene, so carrying out verifying gene table
Up to experiment when, researchers can collect egg mother cell period by microinjection or inhibitor processing and in vitro culture be developed to
Fully expanded blastaea, carries out immunofluorescence dyeing or real-time quantitative fluorescence qPCR does further verifying analysis.Due to capsule
The expansion of embryo chamber, so that blastaea is bigger than general embryo's volume, it, may if be collected blastaea with common shifting embryo needle
Make blastaea shrinkage, the solid shape structure of blastaea script cannot be maintained, has a degree of influence to experimental result.Especially exist
When verifying cell lineage test, embryonic development to blastaea experienced the first time cell point during mammalian development
Change, the trophectoderm adhesion of blastocoele (package) and inner cell mass occur, (what is flocked together in cavity is more
A oval cell) two cell lines in order to verify the expression and positioning that have correlation gene with cell differentiation need to guarantee blastaea
Stereochemical structure is in order to observing the expression form of two cell lines.Blastaea stereochemical structure is wanted when clapping laser co-focusing picture
It asks higher, abundant and clear evidence could be only in this way provided to our experimental result, and ensure the vertical of blastaea
Body structure clearly express in the picture taken.So we need a kind of special shifting embryo needle to be collected capsule
Embryo.The present invention provides a kind of simple and convenient easy system grasped and blastaea stereochemical structure is maintained to move embryo needle easy to operate to collect blastaea
Make method.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of shifting embryo needles for being able to maintain that blastaea stereochemical structure.
The invention solves another technical problem be to provide a kind of mouthful of suction and move embryo device.
The present invention another also to be solved technical problem is to provide a kind of hand-held shifting embryo device.
For moving embryo needle, the technical solution adopted by the present invention is that, a kind of shifting embryo needle being able to maintain that blastaea stereochemical structure moves
Embryo needle includes thin open end and thick open end, and the circular open diameter of thick open end is straight greater than the circular open of thin open end
Diameter；The interior circular diameter of thin open end is 0.20mm~0.28mm, and wall thickness is 0.01mm~0.05mm, and the port of thin open end is flat
It is neat smooth.
It is made preferably, moving embryo needle of thin glass tube.
As further preferred, above-mentioned shifting embryo needle is prepared by the following steps to obtain:
(1) take that a root long degree is 100mm~150mm and cross section is circular straight thin glass tube, the thin glass tube
Interior circular diameter be 0.20mm~0.28mm, wall thickness be 0.01mm~0.05mm；
(2) both hands level holds the both ends of thin glass tube, by the middle part position of thin glass tube in the flame envelope of alcolhol burner
Carry out calcination；
(3) thin glass tube is slowly rotated while calcination, so that thin glass tube is all heated equal by the calcination position whole body
It is even；Until being softened by abundant calcination in the middle part of thin glass tube and being enough moulding；
(4) thin glass tube is quickly withdrawn from from alcolhol burner flame, at the same with the hands by thin glass tube to both sides elongate and in
Between position attenuate, the intermediate increased length of position drawn be 14cm ± 2cm；
(5) cool down to the calcination position of thin glass tube, be placed in a planar object surface, protect thin glass tube both ends
It holds shape in line and prevents from deforming；
(6) thin glass tube is cut in two at the place right in the middle for stretching position with grinding wheel, and every section of thin glass tube becomes one
End is thick open end and the other end is the shifting embryo needle needle embryo of thin open end；The thin open end diameter of needle embryo is measured, thin opening is selected
The interior circular diameter at end is 0.20mm~0.28mm, and the needle embryo that wall thickness is 0.01mm~0.05mm is qualified needle embryo；
(7) the thin open end of qualified needle embryo is modified: first observes the end of the thin open end of needle embryo under the microscope
Whether mouth is concordant, if port clips one section with grinding wheel not concordant is made a new and concordant thin open end again；If thin opening
The thin open end for holding Qi Zeyong needle embryo level with both hands is gently brushed away in smooth frosting, will if scratch occurs in frosting
Thin open end is placed in alcolhol burner internal flame roasting 1~2s and quickly withdraws from, and is drawn again with thin open end in frosting after cooling
It draws, if continuing to repeat aforesaid operations there are also scratch on frosting, until no longer there is scratch in frosting, i.e., thin opening
The port at end is concordantly smooth；
(8) the thick open end of qualified needle embryo is modified: the thick open end of needle embryo is first placed on to the flame envelope of alcolhol burner
It withdraws from after upper slowly rotation 20 ± 5s of calcination, then cools down to calcination position；With hand go to touch thick open end port whether
It is concordant smooth, it is repeated the above steps if unsmooth until the port of thick open end is smooth；So far, a shifting embryo needle finished product production
It completes.
Preferably, if uneven to position calcination among thin glass tube, leading to the needle embryo pulled out in step (3)
Thickness is uneven；In case of the non-uniform situation of calcination, the calcination position of thin glass tube can redden, at this moment slight in flame
Ground moves up and down the position of thin glass tube, until redden position gradually move back into it is colourless, and to thin glass tube calcination position soften
Thin glass tube can be drawn and attenuated immediately after withdrawing from alcolhol burner flame.
Preferably, the calcination duration at position is 18~22 seconds among thin glass tube in step (3).
Preferably, in step (4), as the method for inspection, with the hand horizontally thin glass tube of grab tensile in place
One end illustrates that thin glass tube has reached if the thin glass tube other end hangs down naturally and both ends are at 160 °~175 ° of angle
It is required to stretching.
Preferably, being cooled down in step (5) and step (7) using calcination position of the alcohol swab to thin glass tube.
Preferably, the attenuation velocities at thin glass tube calcination position are preferably slow and stretch small with Etech in step (6).
Embryo device is moved for mouth suction, the technical solution adopted by the present invention is that, a kind of mouthful of suction shifting embryo device, including mouth suction pipe,
Pipetting tip and above-mentioned shifting embryo needle, one end of mouth suction pipe are connect with the thick open end for moving embryo needle, the other end of mouth suction pipe and shifting
The connection of liquid suction nozzle.
Embryo device is moved for hand-held, the technical solution adopted by the present invention is that, a kind of hand-held shifting embryo device, including connecting tube,
The ozzle of syringe and above-mentioned shifting embryo needle, the thick open end and syringe of moving embryo needle is connected by connecting tube.
Preferably, connecting tube is colloid hose.
The beneficial effects of the present invention are:
Provide a kind of specific purpose tool for collecting blastaea and blastaea stereochemical structure can be maintained --- move embryo needle.The shifting embryo
Needle system is made of the common test material being easy to get, and production method is simple and convenient, easy grasp easy to operate.Use the shifting embryo needle energy
Enough greatly improve the working efficiency of blastaea collection.
Detailed description of the invention
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Fig. 1 is the shifting embryo needle construction schematic diagram of the embodiment of the present invention.
Fig. 2 is that the mouth suction of the embodiment of the present invention moves embryo device structural schematic diagram.
Fig. 3 is that the hand-held of the embodiment of the present invention moves embryo device structural schematic diagram.
Marked in the figure: 1- moves embryo needle, the thin open end 101-, the thick open end 102-, 2- mouthfuls of suction pipes, 3- pipetting tip, 4- mistake
Filter chamber, 5- silica gel hose, 6- syringe.
Specific embodiment
Fig. 1 is a kind of shifting embryo needle 1 for being able to maintain that blastaea stereochemical structure, by one section of both ends open and the circular glass of perforation
Straight tube is constituted, and wherein one end is thin open end 101, and the other end is thick open end 102, and the opening diameter of thick open end is greater than
The opening diameter of thin open end.The interior circular diameter for moving the thin open end of embryo needle is 0.20mm, and wall thickness takes 0.02mm, while requiring carefully to open
The port at mouth end is concordant and smooth.The interior circular diameter for moving the thick open end of embryo needle is 2.0mm, outside diameter 2.5mm.
Entire shifting embryo needle, which is attenuated using one whole thin glass tube by appropriate stretching, to be made, therefore shifting embryo needle is from thick opening
End outside diameter 2.5mm gradually decreases to the outside diameter 0.24mm of thin open end, and (circular diameter is in i.e. thin open end
0.20mm), and wall thickness also correspondingly gradually decreases to the 0.02mm of thin open end from the 0.25mm of thick open end.
The present embodiment is using thin glass tube and the shifting embryo needle for meeting above-mentioned specification is prepared according to following step.
1. material
Specification is 2.5 × 2.0 × 100 thin glass tubes (model BJ-40), stereomicroscope (Nikon, SMZ1000), wine
Smart lamp, alcohol swab, grinding wheel, 9cm Tissue Culture Dish or clean smooth plastic plate.
2. moving the production of embryo needle
2.1. needle embryo is drawn
(1) both hands level holds the both ends of thin glass tube, and the intermediate position (2~3cm) of thin glass tube is placed on alcolhol burner
Flame envelope in carry out calcination.
(2) thin glass tube is slowly rotated while calcination, the calcination duration is 18~22 seconds, until in thin glass tube
Portion is softened by abundant calcination and is enough moulding.
(3) thin glass tube is quickly withdrawn from from alcolhol burner flame, and simultaneously with the hands elongates thin glass tube to both sides, it will
Total length becomes 24cm ± 2cm by the thin glass tube of original 10cm after elongating, wherein the increased tensile elongation in intermediate position is
14cm±2cm.The speed and power for drawing needle are controlled, i.e., speed wants slow and power is preferably small.
As a kind of inspection thin glass tube stretch whether satisfactory method, reach both fixed length to thin glass tube drawn
After degree, horizontally hold one end of thin glass tube with hand and the other end can nature hang down, and thin glass tube both ends at 160 °~
175 ° of angle illustrates that thin glass tube has had reached stretching and required.
(4) cooled down with alcohol swab to (i.e. calcination position) at the thickness bore gradual change at both ends, thin glass tube is placed
In desktop, so that thin glass tube is kept into a rectilinear form and prevent its deformation.
(5) thin glass tube is cut in two from the intermediate position drawn and attenuated through calcination with grinding wheel, such one has one end thick
The shifting embryo needle needle embryo of open end and the thin open end of the other end just completes.The thin open end diameter for measuring needle embryo, picks out and carefully opens
It is 0.20mm~0.28mm that mouth end, which meets interior circular diameter, and wall thickness is that the needle embryo of 0.01mm~0.05mm specification enters repairing for next step
Whole, the needle embryo for not meeting this specification is then eliminated.
2.2. needle embryo is modified
(6) next it is exactly to be modified to shifting embryo needle needle embryo, first thick open end is modified, thick open end is placed on
20 ± 5s of calcination is slowly rotated on the flame envelope of alcolhol burner, is withdrawn from and is moved embryo needle, is cooled down with alcohol swab to calcination position, then used
Whether hand goes to touch open end smooth, is repeated the above steps if unsmooth until thick open end is smooth, then again to thin opening
It is modified at end.
(7) the thin open end for moving embryo needle needle embryo is modified, whether the first port for observing thin open end under the microscope
Concordantly, if it find that port is not concordant, new port is exposed after just clipping one section again with grinding wheel, until thin open end is concordant；
It is gently brushed away with the thin open end for moving embryo needle in 9cm Tissue Culture Dish, because the surface of Tissue Culture Dish is smooth plastics
Surface, its surface will appear scratch after thin open end is streaked, and then be placed on thin open end after baking 1~2s in alcolhol burner internal flame
Needle quickly is removed, then is brushed away in 9cm Tissue Culture Dish, if continuing to repeat aforesaid operations, directly there are also scratch on Tissue Culture Dish
To not occurring scratch on Tissue Culture Dish, then illustrate that the port of thin open end is concordantly smooth, such one complete
Embryo needle is moved just to complete.
2.3. points for attention
(1) it in aforesaid operations step (2), has to when carrying out calcination to the intermediate position of thin glass tube while slowly turning
Dynamic thin glass tube, so that the whole body calcination of thin glass tube is uniform, if calcination is uneven, the needle embryo thickness of pull-out is uneven
It is even.In case of the non-uniform situation of calcination, the calcination position of thin glass tube can redden, and at this moment can slightly move up and down thin
The position of glass tube, and needle embryo can be stretched while removing needle to thin glass tube softening.
(2) overlong time centainly is unable to the thick open end for moving embryo needle and the finishing of thin open end, otherwise will cause bore mistake
Carefully, it on the one hand will cause that gas flow optimized is bad when in use in this way, slow down the speed for collecting blastaea, on the other hand blastaea can be made to wrinkle
Contracting.
3. moving the assembling of embryo device
The shifting embryo needle of the present embodiment can be assembled into the shifting embryo device of following two form:
A kind of mouthful of suction as shown in Figure 2 moves embryo device, is that will move embryo needle 1 to combine by mouth suction pipe 2 one with pipetting tip 3
It rises and constitutes mouth suction shifting embryo device.Specific practice is that the thin open end 101 for moving embryo needle 1 is placed in foremost, will move slightly opening for embryo needle 1
One end of 102 insert port suction pipe 2 of mouth end, the other end of mouth suction pipe 2 are connect with pipetting tip 3, are assembled into one and are utilized mouth pair
Pipetting tip Push-pull Flow moves embryo device to control the mouth suction of operation.In order to filter the air of mouth inspiration or discharge, inhaled in mouth
The both ends of pipe 2 are equipped with the filter chamber 4 of filling absorbent cotton.
A kind of hand-held as shown in Figure 3 moves embryo device, is that will move embryo needle 1 to combine by silica gel hose 5 one with syringe 6
It rises and constitutes hand-held shifting embryo device.Specific practice is that the thin open end 101 for moving embryo needle 1 is placed in foremost, will move slightly opening for embryo needle 1
One end of silica gel hose 4 is inserted at mouth end 102, and the other end of silica gel hose 4 is connect with the ozzle of syringe 5, and being assembled into one can
With hand-held shifting embryo device that is hand-held and being controlled by syringe.Due to moving thick open end 102 and the silica gel hose diameter difference of embryo needle 1
It is different larger, therefore the reducer union of one bell shape of intermediate access in the two.
4. technical characterstic
Below for collecting the 7th day pig blastocyst, illustrate that the present embodiment moves the use and advantage of embryo needle.
Utilize the Blastocysts diameter that the 7th day pig is grown to measurement under graduated microscope.Measurement is adopted with microscope
It is the eyepiece ruler of 0.1mm scale, the enlargement ratio of eyepiece is 8, by the calculation formula of eyepiece ruler are as follows: eyepiece ruler scale value ÷
Object lens multiplying power, thus measuring pig blastocyst diameter is 0.16mm~0.25mm.
According to the Blastocysts diameter of the pig measured above, the present embodiment will move the design of embryo needle are as follows: inner circle diameter is
0.20mm~0.28mm, wall thickness are 0.01mm~0.05mm.Using the shifting embryo needle of above-mentioned specification, can easily carry out the 7th day
The collection of pig blastocyst.
From requirement actually from the point of view of, move embryo needle specification in most importantly inner circle diametric requirements than blastaea diameter omit
Greatly, as long as length is adapted with job requirement, workplace and working environment, and the length direction for moving embryo needle slightly has
Bending also can be used.
If the interior circular diameter that production moves embryo needle is excessive or too small, when use, can generate following drawback:
Such as during carrying out immunofluorescence dyeing, when with the verifying blastaea trophectoderm expression experiment of CDX2 antibody, need
Zona-free is carried out to blastaea, (i.e. thin open end diameter is less than above-mentioned rule if the thin open end of shifting embryo needle used is meticulous
Lattice), blastaea can be crimped to one immediately, so that the trophectoderm structure observed required for can't see us；If moving embryo
The thin open end of needle is excessively thick (i.e. thin open end diameter is greater than above-mentioned specification), can not only bring the impurity of coloring into tableting processes
Other excessive liquid can be brought into, impurity may be diffused into the effect of taking pictures in blastaea ambient influence later period, it is also possible to spread
To blastaea position so that do not see the expression of entire blastaea, excessive liquid fluorescence can be made to be easy to be quenched influence is anti-to quench
It goes out the effect of agent, blastaea can also crushed so that piece pressed is from using.For another example when being fluorescent quantitation qPCR, equally
Also extra liquid and impurity can be brought into, on the one hand making to crack not exclusively influences lytic effect, on the other hand influences what RNA was extracted
As a result, it is all inaccurate to eventually lead to entire result.And embryo needle is moved made of the present embodiment then can effectively avoid the above
Variety of problems, keep experimental implementation more convenient succinct, not only solved the problems, such as blastaea collection, but also be able to maintain that the solid of blastaea
Structure substantially increases blastaea collection efficiency.
The production method of the shifting embryo needle of the present embodiment is very simple, uses the most common experimental material.Although blastaea
Various reasons in growth course, can make the size disunity of blastaea, and the shifting embryo needle of the present embodiment can meet above-mentioned rule
In the diameter range of lattice, it is ensured that maintain the stereochemical structure of various diameter blastaeas.This shifting embryo needle collects experimentation in original sample
In maintain the stereochemical structure of blastaea script, just make researchers be able to carry out lower step experimental implementation, so ensure test knot
The accuracy of fruit.So one shifting embryo needle for being able to maintain that blastaea stereochemical structure of production is highly useful and necessary, and this shifting
The production method simple possible of embryo needle and easy to operate is a kind of Simple And Practical method that can carry out extensively.
The embodiments of the present invention described above are not intended to limit the scope of the present invention.It is any in the present invention
Spirit and principle within made modifications, equivalent substitutions and improvements etc., should be included in claim protection model of the invention
Within enclosing.
Claims (10)
1. a kind of shifting embryo needle for being able to maintain that blastaea stereochemical structure, which is characterized in that the shifting embryo needle includes thin open end and thick
Open end, and the circular open diameter of thick open end is greater than the circular open diameter of thin open end；The inner circle of the thin open end
Diameter is 0.20mm~0.28mm, and wall thickness is 0.01mm~0.05mm, and the port of thin open end is concordantly smooth.
2. moving embryo needle as described in claim 1, which is characterized in that be prepared by the following steps to obtain:
(1) take that a root long degree is 100mm~150mm and cross section is circular straight thin glass tube, the thin glass tube it is interior
Circular diameter is 0.20mm~0.28mm, and wall thickness is 0.01mm~0.05mm；
(2) both hands level holds the both ends of thin glass tube, and the middle part position of thin glass tube is carried out in the flame envelope of alcolhol burner
Calcination；
(3) thin glass tube is slowly rotated while calcination, so that thin glass tube is all heated evenly by the calcination position whole body；Directly
Softened in the middle part of to thin glass tube by abundant calcination and is enough moulding；
(4) thin glass tube is quickly withdrawn from from alcolhol burner flame, at the same with the hands by thin glass tube to both sides elongate and middle part
Position attenuates, and the intermediate increased length of position drawn is 14cm ± 2cm；
(5) cool down to the thin glass tube calcination position after stretching, be placed in a planar object surface, make thin glass tube both ends
It is kept into a rectilinear form and prevents from deforming；
(6) thin glass tube is cut in two at the place right in the middle for stretching position with grinding wheel, and every section of thin glass tube is as one end
Thick open end and the other end are the shifting embryo needle needle embryo of thin open end；The thin open end diameter for measuring needle embryo, selects thin open end
Interior circular diameter is 0.20mm~0.28mm, and the needle embryo that wall thickness is 0.01mm~0.05mm is qualified needle embryo；
(7) modify to the thin open end of qualified needle embryo: the port for first observing the thin open end of needle embryo under the microscope is
It is no concordant, if port clips one section with grinding wheel not concordant is made a new and concordant thin open end again；If thin opening is held level with both hands
The thin open end of Qi Zeyong needle embryo is gently brushed away in smooth frosting, if scratch occurs in frosting, will carefully be opened
Mouth end is placed in alcolhol burner internal flame roasting 1~2s and quickly withdraws from, and is brushed away again with thin open end in frosting after cooling, if
There are also scratch on frosting, continue to repeat aforesaid operations, until no longer there is scratch, i.e., the end of thin open end in frosting
Mouth is concordant smooth；
(8) the thick open end of qualified needle embryo is modified: first the thick open end of needle embryo is placed on the flame envelope of alcolhol burner and is delayed
Slow-speed is withdrawn from after moving 20 ± 5s of calcination, then is cooled down to calcination position；Go to the port for touching thick open end whether concordant with hand
It is smooth, it is repeated the above steps if unsmooth until the port of thick open end is smooth；So far, a shifting embryo needle finished product has made
At.
3. moving embryo needle as claimed in claim 2, which is characterized in that in step (3), if to position calcination among thin glass tube
Unevenly, then cause the needle embryo thickness pulled out uneven；In case of the non-uniform situation of calcination, the calcination position of thin glass tube
Can redden, the position of thin glass tube is at this moment slightly moved up and down in flame, until redden position gradually move back into it is colourless, and to
Thin glass tube calcination position, which softens, immediately to be drawn and attenuated thin glass tube after withdrawing from alcolhol burner flame.
4. moving embryo needle as claimed in claim 2, which is characterized in that in step (3), the calcination at position is held among thin glass tube
The continuous time is 18~22 seconds.
5. moving embryo needle as claimed in claim 2, which is characterized in that in step (4), as the method for inspection, horizontally with hand
One end of the thin glass tube of grab tensile in place, if the thin glass tube other end hangs down naturally and both ends are at 160 °~175 ° of folder
Angle illustrates that thin glass tube has reached stretching and requires.
6. moving embryo needle as claimed in claim 2, which is characterized in that in step (5) and step (7), using alcohol swab to thin
The calcination position of glass tube cools down.
7. moving embryo needle as claimed in claim 2, which is characterized in that in step (6), the drawing-down speed at thin glass tube calcination position
Degree is preferably slow and stretches small with Etech.
8. a kind of mouthful of suction moves embryo device, which is characterized in that inhaled including moving embryo needle, mouth suction pipe and liquid relief as described in claim 1
Head, one end and connection, the other end of the mouth suction pipe of the thick open end for moving embryo needle and mouth suction pipe are connect with pipetting tip.
9. a kind of hand-held moves embryo device, which is characterized in that including moving embryo needle, connecting tube and syringe as described in claim 1,
The ozzle of the thick open end for moving embryo needle and syringe is connected by connecting tube.
10. hand-held as claimed in claim 9 moves embryo device, which is characterized in that the connecting tube is colloid hose.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910426993.1A CN110013292A (en) | 2019-05-21 | 2019-05-21 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910426993.1A CN110013292A (en) | 2019-05-21 | 2019-05-21 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
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| CN110013292A true CN110013292A (en) | 2019-07-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910426993.1A Pending CN110013292A (en) | 2019-05-21 | 2019-05-21 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
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| CN203898495U (en) * | 2014-03-31 | 2014-10-29 | 中国医学科学院医学生物学研究所 | Embryo transfer device for rat and mouse embryo transfer |
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| CN203898495U (en) * | 2014-03-31 | 2014-10-29 | 中国医学科学院医学生物学研究所 | Embryo transfer device for rat and mouse embryo transfer |
| CN203988494U (en) * | 2014-08-22 | 2014-12-10 | 黑龙江省畜牧研究所 | For beef cattle embryo transfer mouthful suction embryo manipulation device |
| WO2016101226A1 (en) * | 2014-12-25 | 2016-06-30 | 河北农业大学 | Embryo transplantation device and operation method thereof |
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