CN210301174U - Embryo moving needle and embryo moving device capable of maintaining three-dimensional structure of blastocyst - Google Patents
Embryo moving needle and embryo moving device capable of maintaining three-dimensional structure of blastocyst Download PDFInfo
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- CN210301174U CN210301174U CN201920736237.4U CN201920736237U CN210301174U CN 210301174 U CN210301174 U CN 210301174U CN 201920736237 U CN201920736237 U CN 201920736237U CN 210301174 U CN210301174 U CN 210301174U
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Abstract
The utility model discloses a can maintain embryo spatial structure move embryo needle and move embryo ware. The embryo moving needle comprises a thin opening end and a thick opening end, and the opening diameter of the thick opening end is larger than that of the thin opening end; the diameter of the inner circle of the thin opening end is 0.20 mm-0.28 mm, the wall thickness is 0.01 mm-0.05 mm, and the end opening of the thin opening end is level and smooth. The thick opening end of the embryo moving needle is connected with one end of a mouth suction pipe, and the other end of the mouth suction pipe is connected with a liquid-transferring suction head, so that the mouth suction type embryo moving device is formed by assembling. The wide open end of the embryo moving needle is connected with the nozzle of the injector through a connecting pipe, thus forming the hand-held embryo moving device. The utility model provides a special tool-embryo moving needle for collecting blastocysts and maintaining the three-dimensional structure of the blastocysts. The embryo transfer needle is made of common and easily available test materials, and the manufacturing method is simple, convenient and fast, and easy to operate and master. The embryo transferring needle can greatly improve the work efficiency of blastocyst collection.
Description
Technical Field
The utility model relates to a can maintain embryo spatial structure move embryo needle to and mouth is inhaled formula and is moved embryo ware and hand-held type and move embryo ware.
Background
In recent years, with the vigorous development of embryo engineering technology, embryo transplantation technology is becoming mature increasingly, and in the aspect of reproductive medicine, more and more patients with infertility choose to perform 'test tube babies', namely, sperms and ova are fertilized in vitro to develop into embryos and then transferred into maternal uterus to develop into fetuses. Typically, embryos that develop to the cleavage stage or the blastocyst stage will be selected for transfer. With the development of embryo culture systems and many years of clinical experience, there is a greater tendency to select blastocysts for embryo transfer. Firstly, the blastocyst is developed further on the basis of the embryo in the cleavage stage, the embryo is optimized again in the process, and the implantation of the embryo in the parent body is more convenient; in addition, the blastocyst has stronger activity and adaptability than the embryo in the cleavage stage, only 1-2 blastocysts need to be transplanted in the embryo transplantation process, and the incidence rate of multiple pregnancy is reduced; more importantly, embryo cleavage occurs in the oviduct under the physiological state of normal in vivo fertilization, and the embryo enters the uterus for implantation after the development of the blastocyst stage, so that the embryo implantation can be quickly realized by selecting blastocyst transplantation, the opportunity of embryo migration in the oviduct is reduced, and the incidence rate of extrauterine pregnancy is reduced. Because the blastocyst has strong capability of repairing freezing damage and small influence on the development potential of embryos, the blastocyst culture occupies an increasingly important position in an embryo culture system and an embryo transplantation process. In the field of animal husbandry production, people also often perform in vitro fertilization and embryo transplantation on livestock for breeding and breed conservation, and although embryo development processes of different animals are different, more blastocysts are selected for transplantation in the embryo transplantation process, and since test-tube infants are developed on the basis of researching physiological mechanisms of various animals, the blastocyst culture of animals is favored by people.
The formation of the blastocyst is morphologically subjected to cell division, cell densification, the appearance of the blastocyst cavity and the expansion process of the blastocyst cavity, and gene regulation is converted from maternal factor regulation to embryo gene regulation, so that during the experiment of verifying gene expression, researchers can collect the blastocyst which is subjected to microinjection or inhibitor treatment in the oocyte period and is cultured and developed in vitro to be completely expanded, and perform immunofluorescence staining or real-time quantitative fluorescence qPCR for further verification and analysis. Because of the expansion of the cavity of the blastocyst, the volume of the blastocyst is larger than that of the common blastocyst, and if a common blastocyst transferring needle is used for collecting the blastocyst, the blastocyst can be shrunk, the original three-dimensional morphological structure of the blastocyst cannot be maintained, and the experimental result is influenced to a certain degree. Particularly, in the test for verifying cell lineage differentiation, the embryo develops to a blastocyst and undergoes the first cell differentiation in the development process of mammals, two cell lines of trophectoderm (a monolayer of epithelial cells wrapping the cavity of the blastocyst) and inner cell mass (a plurality of oval cells gathered together in the cavity) appear, and in order to verify the expression and positioning of genes related to cell differentiation, the three-dimensional structure of the blastocyst needs to be ensured so as to observe the expression forms of the two cell lines. The requirement on the three-dimensional structure of the blastocyst is higher when a laser confocal picture is shot, sufficient and clear evidence can be provided for an experimental result of people only, and the three-dimensional structure of the blastocyst can be clearly expressed in the shot picture. Therefore, a special embryo transferring needle is needed for collecting blastocysts. The utility model provides a simple, convenient and easy-to-operate manufacturing method for embryo moving needle which is easy to master and maintain the three-dimensional structure of blastocyst.
Disclosure of Invention
The to-be-solved technical problem of the utility model is to provide a can maintain embryo three-dimensional structure move embryo needle.
The to-be-solved technical problem of the utility model is to provide an embryo ware is moved to mouth suction type.
The utility model discloses still another technical problem who solves provides a hand-held type moves embryo ware.
For embryo moving needle, the technical scheme adopted by the utility model is that the embryo moving needle capable of maintaining the three-dimensional structure of the blastocyst comprises a thin opening end and a thick opening end, and the diameter of the circular opening of the thick opening end is larger than that of the circular opening of the thin opening end; the diameter of the inner circle of the thin opening end is 0.20 mm-0.28 mm, the wall thickness is 0.01 mm-0.05 mm, and the end opening of the thin opening end is level and smooth.
Preferably, the embryo transfer needle is made of a thin glass tube.
To the mouth inhale the formula and move embryo ware, the technical scheme of the utility model be, a mouth inhale the formula and move embryo ware, including mouth straw, move liquid suction head and foretell embryo needle that moves, the one end of mouth straw is connected with the thick open end that moves embryo needle, and the other end of mouth straw is connected with moving liquid suction head.
Preferably, the embryo transfer needle is made of a thin glass tube.
To the hand-held type embryo ware that moves, the utility model discloses a technical scheme be, a hand-held type moves embryo ware, including connecting pipe, syringe and foretell embryo needle that moves, the thick open end that moves embryo needle passes through the connecting pipe with the mouthpiece of syringe and is connected.
Preferably, the embryo transferring needle is made of a thin glass tube, and the connecting tube is a rubber hose.
The utility model has the advantages that:
provides a special tool, namely a embryo transferring needle, which is used for collecting the blastula and can maintain the three-dimensional structure of the blastula. The embryo transfer needle is made of common and easily available test materials, and the manufacturing method is simple, convenient and fast, and easy to operate and master. The embryo transferring needle can greatly improve the work efficiency of blastocyst collection.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Fig. 1 is a schematic structural view of a embryo transfer needle according to an embodiment of the present invention.
Fig. 2 is a schematic structural view of a mouth-suction type embryo transfer device according to an embodiment of the present invention.
Fig. 3 is a schematic structural view of a handheld embryo transfer device according to an embodiment of the present invention.
The labels in the figure are: 1-embryo transferring needle, 101-thin open end, 102-thick open end, 2-mouth suction pipe, 3-liquid transferring suction head, 4-filtering cavity, 5-silica gel hose and 6-injector.
Detailed Description
FIG. 1 shows a embryo transfer needle 1 capable of maintaining the three-dimensional structure of a blastocyst, which is composed of a straight circular glass tube having both ends open and penetrating, wherein one end is a thin open end 101, the other end is a thick open end 102, and the opening diameter of the thick open end is larger than that of the thin open end. The diameter of the inner circle of the thin opening end of the embryo transferring needle is 0.20mm, the wall thickness is 0.02mm, and meanwhile, the port of the thin opening end is required to be parallel and smooth. The diameter of the inner circle of the thick opening end of the embryo transferring needle is 2.0mm, and the diameter of the outer circle is 2.5 mm.
1. Preparing a embryo transfer needle:
a whole thin glass tube (model is BJ-40) with the length of 100mm, the diameter of the outer circle of 2.5mm and the diameter of the inner circle of 2.0mm is adopted, two hands horizontally hold two ends of the thin glass tube, the middle part of the thin glass tube is placed in the outer flame of an alcohol lamp to be burnt and softened, and then the thin glass tube is properly stretched and thinned to prepare the embryo moving needle meeting the requirement of the thin opening end. Therefore, the embryo transferring needle is gradually reduced from the outer circle diameter of the thick opening end to the outer circle diameter of the thin opening end by 2.5mm (namely, the inner circle diameter of the thin opening end is 0.20mm), and the wall thickness is correspondingly gradually reduced from the outer circle diameter of the thick opening end to the outer circle diameter of the thin opening end by 0.25mm to the outer circle diameter of the thin opening end by 0.02 mm.
2. Assembly of embryo transfer device
The embryo transfer needle of the embodiment can be assembled into the following two forms of embryo transfer devices:
a mouth-suction embryo transfer device as shown in FIG. 2 is composed of an embryo transfer needle 1 and a liquid transfer suction head 3 which are combined together through a mouth suction pipe 2. The specific assembling method is that the thin open end 101 of the embryo moving needle 1 is arranged at the forefront, the thick open end 102 of the embryo moving needle 1 is inserted into one end of the oral pipette 2, the other end of the oral pipette 2 is connected with the liquid-transfer pipette tip 3, and the oral suction type embryo moving device which controls the operation by blowing and sucking air flow to the liquid-transfer pipette tip through a mouth is assembled. In order to filter the air sucked or discharged by the mouth, a filter chamber 4 filled with absorbent cotton is arranged at the two ends of the suction pipe 2.
A hand-held embryo transfer device as shown in fig. 3 is formed by combining an embryo transfer needle 1 and an injector 6 through a silica gel hose 5. The specific assembling method is that the thin open end 101 of the embryo moving needle 1 is arranged at the forefront, the thick open end 102 of the embryo moving needle 1 is inserted into one end of the silica gel hose 4, the other end of the silica gel hose 4 is connected with the nozzle at the front end of the injector 5, and the handheld embryo moving device which can be held by hand and controlled by the injector is assembled. Because the diameter difference between the thick opening end 102 of the embryo moving needle 1 and the silica gel hose is large, a bell-mouth-shaped reducing joint is connected between the thick opening end and the silica gel hose.
3. Technical characteristics
The technical features of the embryo transfer needle of this embodiment will be described below by taking the collection of day seven pig blastocysts as an example.
The expanded blastocyst diameter of the pigs grown to day seven was measured using a microscope with a scale. What the microscope used for measurement adopted is the eyepiece chi of 0.1mm scale, and the magnification of eyepiece is 8, and the computational formula according to the eyepiece chi is: the diameter of the pig blastocyst is measured by dividing the scale value of an ocular lens ruler by the multiplying power of an objective lens, and is 0.16 mm-0.25 mm.
Based on the above measured expanded blastocyst diameters of the pigs, the present example designed the embryo-moving needle to: the diameter of the inner circle is 0.20 mm-0.28 mm, and the wall thickness is 0.01 mm-0.05 mm. By using the embryo transferring needle with the specification, the collection of the pig blastocysts on the seventh day can be conveniently carried out.
From the practical aspect of use requirements, the most important of the specifications of the embryo transferring needle is that the diameter of the inner circle is slightly larger than that of the blastocyst, the length of the embryo transferring needle is only required to be suitable for the working requirement, the working place and the working environment, and the embryo transferring needle can also be used by being slightly bent in the length direction.
If the diameter of the inner circle of the embryo transferring needle is too large or too small, the following disadvantages can be caused during use:
when the CDX2 antibody is used for verifying blastocyst trophectoderm expression experiments in the immunofluorescence staining process, a blastocyst needs to be subjected to a zona pellucida, and if the thin open end of the embryo transfer needle used is too thin (namely the diameter of the thin open end is smaller than the specification), the blastocyst can be immediately shrunk into a mass, so that the trophectoderm structure which is required to be observed by people cannot be observed; if the thin opening end of the embryo transferring needle is too thick (namely, the diameter of the thin opening end is larger than the specification), not only colored impurities but also excessive other liquid can be brought in during tabletting, the impurities can be diffused to the periphery of the blastocyst to influence the later photographing effect and the position of the blastocyst, so that the expression condition of the whole blastocyst can not be seen clearly, the excessive liquid can easily quench fluorescence to influence the effect of the anti-quenching agent, and the blastocyst can be crushed to be unusable. In addition, when the quantitative qPCR is performed, redundant liquid and impurities are also brought in, so that on one hand, the cracking effect is not completely influenced by cracking, on the other hand, the result of RNA extraction is influenced, and finally, the whole result is inaccurate. The embryo moving needle manufactured by the embodiment can effectively avoid the problems, so that the experimental operation is more convenient and simpler, the problem of blastocyst collection is solved, the three-dimensional structure of the blastocyst can be maintained, and the blastocyst collection efficiency is greatly improved.
The embryo transfer needle of the embodiment is very simple in manufacturing method, and the most common experimental materials are used. Although the size of the blastocysts is not uniform due to various reasons in the process of blastocyst development, the embryo transfer needle of the present embodiment can maintain the three-dimensional structure of the blastocysts of various diameters within the diameter range satisfying the above specifications. The embryo moving needle maintains the original three-dimensional structure of the blastocyst in the initial sample collection experiment process, so that researchers can carry out the next experiment operation, and the accuracy of the experiment result is further ensured. Therefore, the embryo transferring needle capable of maintaining the three-dimensional structure of the blastocyst is very useful and necessary to manufacture, and the manufacturing method of the embryo transferring needle is simple and feasible and easy to operate, and is a simple and practical method which can be widely popularized.
The above-mentioned embodiments of the present invention do not limit the scope of the present invention. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (7)
1. The embryo moving needle capable of maintaining the three-dimensional structure of a blastocyst is characterized by comprising a thin opening end and a thick opening end, wherein the diameter of a circular opening of the thick opening end is larger than that of the circular opening of the thin opening end; the diameter of the inner circle of the thin opening end is 0.20 mm-0.28 mm, the wall thickness is 0.01 mm-0.05 mm, and the end opening of the thin opening end is parallel and smooth.
2. The embryo transfer needle of claim 1 wherein the embryo transfer needle is made of a thin glass tube.
3. The mouth-suction type embryo transfer device is characterized by comprising the embryo transfer needle, a mouth suction pipe and a liquid transfer suction head, wherein the embryo transfer needle is as claimed in claim 1, the thick opening end of the embryo transfer needle is connected with one end of the mouth suction pipe, and the other end of the mouth suction pipe is connected with the liquid transfer suction head.
4. The mouth-suction embryo transfer device according to claim 3, wherein the embryo transfer needle is made of a thin glass tube.
5. A hand-held embryo transfer device is characterized by comprising the embryo transfer needle as claimed in claim 1, a connecting pipe and an injector, wherein the wide open end of the embryo transfer needle is connected with the nozzle of the injector through the connecting pipe.
6. The hand-held embryo-moving device according to claim 5, wherein the embryo-moving needle is made of a thin glass tube.
7. The hand-held embryo-transfer device according to claim 5, wherein the connecting tube is a rubber hose.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920736237.4U CN210301174U (en) | 2019-05-21 | 2019-05-21 | Embryo moving needle and embryo moving device capable of maintaining three-dimensional structure of blastocyst |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920736237.4U CN210301174U (en) | 2019-05-21 | 2019-05-21 | Embryo moving needle and embryo moving device capable of maintaining three-dimensional structure of blastocyst |
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| CN210301174U true CN210301174U (en) | 2020-04-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110013292A (en) * | 2019-05-21 | 2019-07-16 | 安徽农业大学 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110013292A (en) * | 2019-05-21 | 2019-07-16 | 安徽农业大学 | A kind of shifting embryo needle being able to maintain that blastaea stereochemical structure |
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