CN109223143A - A kind of embryo transplanter for test-tube baby - Google Patents

A kind of embryo transplanter for test-tube baby Download PDF

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CN109223143A
CN109223143A CN201811331453.7A CN201811331453A CN109223143A CN 109223143 A CN109223143 A CN 109223143A CN 201811331453 A CN201811331453 A CN 201811331453A CN 109223143 A CN109223143 A CN 109223143A
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embryo
storehouse
pipeline
protection liquid
test
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CN109223143B (en
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李玉新
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Wang Aihua
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Weifang Nursing Occupational Institute
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Abstract

The invention discloses a kind of embryo transplanters for test-tube baby, are related to gynaecology's family planning technical field, including fixed link, support handle, hose, hollow shaft, pipeline one, pipeline two;Rack rails one is driven to move upwards by rotate gear two, to drive pushing ram two to move upwards, liquid is protected to be introduced into pipeline one from pipeline two, then hose front end is reached, when liquid to be protected is overflowed from needle position, syringe needle is pierced into inside maternal uterine by rotate gear one, when syringe needle is pierced into maternal uterine, rotating wheel disk makes inside the extended needle of hose head, it pushes after promoting button discharge embryo storehouse internal liquid, rotate gear two makes after protecting liquid storehouse remaining liq to be all discharged again, remove the present apparatus, the present invention greatly enhances protection of the embryo transfer in the process to embryo, so as to avoid the injury during embryo transfer to embryo.

Description

A kind of embryo transplanter for test-tube baby
Technical field
The present invention relates to gynaecology's family planning technical fields, are specifically related to a kind of embryo transplanter for test-tube baby.
Background technique:
" test-tube baby " often is " In vitro fertilization-embryo transplantation " (IVF-ET) China is civil.And in fact, body Outer fertilization is a kind of special technology, is that ovum and sperm are all taken to allow the environment of their manual controls in vitro in vitro Middle completion fertilization process, is then transplanted to body early embryo in the uterus of women, breeds in uterus as child.Using external The baby that fertilization technique generates is known as test-tube baby, these children are also to grow up in the intrauterine of mother.It can be said that " test tube Baby's technology " is equal to " in vitro fertilization.
In the treatment means of sterile disease, test-tube baby is to common are one of effect means.But in actual operation, Since the tool used when embryo transfer is improper, thus cause cannot will to have cultivated successful embryo transfer enters inside uterus and It leads to the failure.Especially for those parents with cervical canal deformity, embryo is sent into son by the cervical canal of deformity It is just more difficult in uterine cavity, it selects most healthy embryo's injection and becomes pregnant into maternal uterine, do not allow because selecting healthy embryo Easily, so the safety of embryo is critically important during injection, but embryo injection tool is too simple at present, safety coefficient compared with It is low.
Summary of the invention
Against the above technical problems, the present invention provides a kind of embryo transplanters for test-tube baby.
The technical scheme is that
A kind of embryo transplanter for test-tube baby, including fixed link, support handle, hose, hollow shaft, pipeline one, pipeline Two, the fixed link front end is connected with fixed disk by card slot, and channel is transversely provided in the middle part of fixed link, and the channel interior is set There is syringe needle, rack rails one is equipped on the downside of the syringe needle rear end, lower end is rotatably connected to gear by rotating bar in the middle part of the fixed link One, one engaged transmission of the gear one and rack rails, the fixed rod rear end is rotatably connected to wheel disc by the hollow shaft, described Support asks the rear side upper end of handle to set embryo storehouse, is connected with embryo storehouse below the embryo storehouse being connected to below the rear end of fixed link Propulsion device, is fixed with protection liquid storehouse below the embryo storehouse, and the protection liquid storehouse lower end, which is connected with protection liquid storehouse and promotes, to be filled Set, front end is connected with gear two by rotating bar by support, gear two and protection liquid storehouse propulsion device engaged transmission, before the hose End is laterally set on needle wall, is wrapped on wheel disc in the middle part of hose, hose end and hollow axis connection, embryo storehouse and hollow shaft Between communicated by the pipeline one, pass through the pipeline two between the protection liquid storehouse and pipeline one and communicate.
Further, the fixed disk includes disk body, centre bore, sucker, connecting column, and the centre bore is located at the disk body Center, there are four the connecting columns, and four connecting columns are uniformly distributed circumferentially in disk body front end, and there are four the suckers, four A sucker is separately fixed at the front end of connecting column, reduces syringe needle in the intracorporal movement of parent by fixed disk.
Further, the wheel disk center is fixedly connected with hollow shaft, and the left end of hollow shaft passes through card slot and one turn of pipeline The right end of dynamic connection, hollow shaft is equipped with limited post, and the right end of the limited post is fixedly connected with the inner wall of fixed link, limited post Left end, by wheel disc by flexible pipe extending needle wall, avoids syringe needle from scratching embryo for limiting to hollow shaft.
Further, embryo storehouse propulsion device includes gasket one, pushing ram one, promotes button, the gasket One is located at the lower end inside embryo storehouse, and one upper end of pushing ram is fixedly connected with gasket one, and one lower end of pushing ram passes through support Outer wall is fixedly connected with the propulsion button, by promoting button that embryo storehouse internal liquid is quickly discharged.
Further, protection liquid storehouse propulsion device includes gasket two, pushing ram two, strip groove, sheet metal, rack rails Two, the gasket two is located at the lower end inside protection liquid storehouse, and two upper end of pushing ram is fixedly connected with gasket two, described Strip groove is located at the right side of gear two, and the rack rails two is located inside strip groove vertically, and the sheet metal is fixed on bar shaped vertically The right side inner wall of slot, the lower end left side of pushing ram two pass through sheet metal lower end and are fixedly connected with two lower end of rack rails, pass through gear two Accurately control the discharge rate of protection liquid.
Further, the embryo storehouse is for nutrient solution needed for storing embryo and embryonic development, the nutrient solution by with Lower weight percent at being grouped as: 0.02% penicillin, compound amido acid injection 5%, human serum albumin 6.3%, chlorination Potassium 1.2%, magnesium sulfate 0.5%, glucose 6%, gentamicin 0.08%, ethylenediamine tetra-acetic acid 3.2%, Sodium Pyruvate 2.2%, Alanyl glutamine 1.5%, taurine 0.85%, D-glucitol 1.2%, calcium chloride 0.9%, sodium dihydrogen phosphate 0.5%, phosphorus Acid dihydride potassium 0.7%, sodium carbonate 5%, lipoic acid 6%, n-acetyl-L-cysteine 3.2%, surplus are water, enhance embryo Activity is become pregnant probability to enhance parent.
Further, protection liquid storehouse internal injection has protection liquid, the protection liquid by following weight percent at It is grouped as: creatinine 0.84%, vernix caseosa linoleic acid 0.54%, beta carotene 0.5%, palmitinic acid 0.8%, L-Glutamine 0.45%, polylactic-co-glycolic acid 0.32%, beta -mercaptoethanol 0.16%, human serum albumin 2.3%, vitamin B1.2%, epidermal growth factor 0.02% and insulin 0.12%, hydrocortisone 0.23,;Cortisol 0.04%, progesterone 0.94%, L-Glutamine 0.74%, HEPES0.84% stimulate parent by protection liquid, carry out pregnancy reaction.
Further, the pH of the described nutrient solution sum is 6.9-7.2, osmotic pressure 312-322KPa, it is ensured that embryo health hair It educates.
Further, the pH of the protection liquid is 6.9-7.2, osmotic pressure 312-322KPa, it is ensured that embryo health development.
Working method of the invention:
S1: determining injection position by inspection equipment, embryo and its nutrient solution be injected into embryo storehouse by syringe, will Protection liquid is injected into inside protection liquid storehouse, and embryo storehouse is discharged by pushing ram one and pushing ram two and protects the air in liquid storehouse, Then fixed disk is adjacent at the skin of parent injection position.
S2: driving rack rails one to move upwards by rotate gear two, so that pushing ram two be driven to move upwards, protection liquid from Pipeline two is introduced into pipeline one, then reaches hose front end, when liquid to be protected is overflowed from needle position, rotate gear one is by syringe needle It is pierced into inside maternal uterine, when syringe needle is pierced into maternal uterine, rotating wheel disk makes inside the extended needle of hose head, pushes to promote and press After embryo storehouse internal liquid is discharged in button, rotate gear two makes after protecting liquid storehouse remaining liq to be all discharged again, removes the present apparatus.
The beneficial effects of the present invention are:
The present invention pushes the nutrient solution comprising embryo to advance to maternal uterine by protection liquid, after syringe needle enters intrauterine, Made inside the extended needle of hose head by rotating wheel disk, in this way when extracting syringe needle, syringe needle avoided to scratch embryonic cell, And influence the health condition of embryo, fixed disk can stabilising arrangement, avoid syringe needle from generating in maternal and be displaced by a relatively large margin, from And uterus inner wall is scratched, the present invention greatly enhances protection of the embryo transfer in the process to embryo, so as to avoid embryo's shifting To the injury of embryo during plant.
Detailed description of the invention
Fig. 1 is the structure chart of invention;
Fig. 2 is the structure chart at invention wheel disc;
Fig. 3 is the left view of the fixed disk of invention;
Fig. 4 is the sheet metal of invention and the connection figure of pushing ram two.
Wherein, 1- fixed link, 2- hold in the palm handle, 3- hose, 4- hollow shaft, 5- pipeline one, 6- pipeline two, 11- fixed disk, 12- Channel, 13- syringe needle, 14- rack rails one, 15- rotating bar, 16- gear one, 17- wheel disc, 21- embryo storehouse, 22- embryo storehouse promote dress Set, 23- protection liquid storehouse, 24- protection liquid storehouse propulsion device, 25- gear two, 111- disk body, 112- centre bore, 113- sucker, 114- connecting column, 41- limited post, 221- gasket one, 222- pushing ram one, 223- promote button, 241- gasket two, 242- Pushing ram two, 243- strip groove, 244- sheet metal, 245- rack rails two.
Specific embodiment
To be further explained explanation below with reference to Fig. 1-2, embodiment is simultaneously convenient for the understanding to technical solution of the present invention Do not constitute limiting the scope of the present invention.
Embodiment 1:
As shown in Figure 1, a kind of embryo transplanter for test-tube baby, including fixed link 1, support are 2, hose 3, hollow shaft 4, pipeline 1, pipeline 26,1 front end of fixed link are connected with fixed disk 11 by card slot, as shown in figure 3, fixed disk 11 includes disk Body 111, centre bore 112, sucker 113, connecting column 114, centre bore 112 are located at the center of disk body 111, and there are four connecting columns 114, Four connecting columns 114 are uniformly distributed circumferentially in 111 front end of disk body, and there are four suckers 113, and four suckers 113 are separately fixed at The front end of connecting column 114 reduces syringe needle in the intracorporal movement of parent by fixed disk, and the middle part of fixed link 1 is transversely provided with channel 12, it is equipped with syringe needle 13 inside channel 12, is equipped with rack rails 1 on the downside of 13 rear end of syringe needle, 1 middle part lower end of fixed link passes through rotating bar 15 are rotatably connected to gear 1, and one 14 engaged transmission of gear 1 and rack rails, 1 rear end of fixed link is connected by the rotation of hollow shaft 4 It is connected to wheel disc 17, as shown in Fig. 2, 17 center of wheel disc is fixedly connected with hollow shaft 4, the left end of hollow shaft 4 passes through card slot and pipeline The right end of one 5 rotation connections, hollow shaft 4 is equipped with limited post 41, and the right end of limited post 41 is fixedly connected with the inner wall of fixed link 1, The left end of limited post 41, by wheel disc by flexible pipe extending needle wall, avoids syringe needle to embryo for limiting to hollow shaft 4 It scratches, support is connected to below the rear end of fixed link 12, and 2 rear side upper end is set embryo storehouse 21 by support, is connected below embryo storehouse 21 There is embryo storehouse propulsion device 22, embryo storehouse propulsion device 22 includes gasket 1, pushing ram 1, promotes button 223, close Packing 1 is located at the lower end inside embryo storehouse 21, and one 222 upper end of pushing ram is fixedly connected with gasket 1, pushing ram one 222 lower ends pass through support and 2 outer walls are fixedly connected with button 223 is promoted, by promoting button that embryo storehouse internal liquid is quickly discharged, The lower section in embryo storehouse 21 is fixed with protection liquid storehouse 23, and embryo storehouse 21 is for nutrient solution needed for storing embryo and embryonic development, battalion Nutrient solution enhances embryo viability, becomes pregnant probability to enhance parent, and protection 23 internal injection of liquid storehouse has protection liquid, and protection liquid stimulation is female Body carries out pregnancy reaction, and protecting the pH of liquid is 6.9-7.2, and osmotic pressure 312-322KPa, the pH of nutrient solution sum are 6.9-7.2, Osmotic pressure is 312-322KPa, it is ensured that embryo health development, it is ensured that embryo health development, protection 23 lower end of liquid storehouse are connected with protection Liquid storehouse propulsion device 24, protection liquid storehouse propulsion device 24 include gasket 2 241, pushing ram 2 242, strip groove 243, sheet metal 244, rack rails 2 245, gasket 2 241 are located at the lower end inside protection liquid storehouse 23,2 242 upper end of pushing ram and gasket two 241 are fixedly connected, and strip groove 243 is located at the right side of gear 2 25, and rack rails 2 245 is located inside strip groove 243 vertically, sheet metal 244 are fixed on the right side inner wall of strip groove 243 vertically, as shown in figure 4, passing through sheet metal 244 on the left of the lower end of pushing ram 2 242 Lower end is fixedly connected with 2 245 lower end of rack rails, the discharge rate of protection liquid is accurately controlled by gear two, support is 2 front ends by turning Lever 15 is connected with gear 2 25, and 24 engaged transmission of gear 2 25 and protection liquid storehouse propulsion device, 3 front end of hose is laterally set on Inside syringe needle 13, be wrapped in the middle part of hose 3 on wheel disc 17,3 end of hose is connect with hollow shaft 4, embryo storehouse 21 and hollow shaft 4 it Between communicated by pipeline 1, protect and communicated between liquid storehouse 23 and pipeline 1 by pipeline 26.
Embodiment 2:
As shown in Figure 1, a kind of embryo transplanter for test-tube baby, including fixed link 1, support are 2, hose 3, hollow shaft 4, pipeline 1, pipeline 26,1 front end of fixed link are connected with fixed disk 11 by card slot, as shown in figure 3, fixed disk 11 includes disk Body 111, centre bore 112, sucker 113, connecting column 114, centre bore 112 are located at the center of disk body 111, and there are four connecting columns 114, Four connecting columns 114 are uniformly distributed circumferentially in 111 front end of disk body, and there are four suckers 113, and four suckers 113 are separately fixed at The front end of connecting column 114 reduces syringe needle in the intracorporal movement of parent by fixed disk, and the middle part of fixed link 1 is transversely provided with channel 12, it is equipped with syringe needle 13 inside channel 12, is equipped with rack rails 1 on the downside of 13 rear end of syringe needle, 1 middle part lower end of fixed link passes through rotating bar 15 are rotatably connected to gear 1, and one 14 engaged transmission of gear 1 and rack rails, 1 rear end of fixed link is connected by the rotation of hollow shaft 4 It is connected to wheel disc 17, as shown in Fig. 2, 17 center of wheel disc is fixedly connected with hollow shaft 4, the left end of hollow shaft 4 passes through card slot and pipeline The right end of one 5 rotation connections, hollow shaft 4 is equipped with limited post 41, and the right end of limited post 41 is fixedly connected with the inner wall of fixed link 1, The left end of limited post 41, by wheel disc by flexible pipe extending needle wall, avoids syringe needle to embryo for limiting to hollow shaft 4 It scratches, support is connected to below the rear end of fixed link 12, and 2 rear side upper end is set embryo storehouse 21 by support, is connected below embryo storehouse 21 There is embryo storehouse propulsion device 22, embryo storehouse propulsion device 22 includes gasket 1, pushing ram 1, promotes button 223, close Packing 1 is located at the lower end inside embryo storehouse 21, and one 222 upper end of pushing ram is fixedly connected with gasket 1, pushing ram one 222 lower ends pass through support and 2 outer walls are fixedly connected with button 223 is promoted, by promoting button that embryo storehouse internal liquid is quickly discharged, The lower section in embryo storehouse 21 is fixed with protection liquid storehouse 23, and embryo storehouse 21 is for nutrient solution needed for storing embryo and embryonic development, battalion Nutrient solution is by following weight percent at being grouped as: 0.02% penicillin, compound amido acid injection 5%, human serum albumin 6.3%, potassium chloride 1.2%, magnesium sulfate 0.5%, glucose 6%, gentamicin 0.08%, ethylenediamine tetra-acetic acid 3.2%, acetone Sour sodium 2.2%, alanyl glutamine 1.5%, taurine 0.85%, D-glucitol 1.2%, calcium chloride 0.9%, biphosphate Sodium 0.5%, potassium dihydrogen phosphate 0.7%, sodium carbonate 5%, lipoic acid 6%, n-acetyl-L-cysteine 3.2%, surplus are Water enhances embryo viability, becomes pregnant probability to enhance parent, and protection 23 internal injection of liquid storehouse has protection liquid, protects liquid by following Weight percent at being grouped as: creatinine 0.84%, vernix caseosa linoleic acid 0.54%, beta carotene 0.5%, palmitinic acid 0.8%, L-Glutamine 0.45%, polylactic-co-glycolic acid 0.32%, beta -mercaptoethanol 0.16%, human serum albumin 2.3%L, Vitamin B1 .2%, epidermal growth factor 0.02% and insulin 0.12%, hydrocortisone 0.23%, cortisol 0.04%, Progesterone 0.94%, L-Glutamine 0.74%, HEPES0.84% stimulate parent by protection liquid, carry out pregnancy reaction, protect The pH for protecting liquid is 6.9-7.2, and osmotic pressure 312-322KPa, the pH of nutrient solution sum are 6.9-7.2, osmotic pressure 312- 322KPa, it is ensured that embryo health development, it is ensured that embryo health development, protection 23 lower end of liquid storehouse are connected with protection liquid storehouse propulsion device 24, protection liquid storehouse propulsion device 24 includes gasket 2 241, pushing ram 2 242, strip groove 243, sheet metal 244, rack rails two 245, gasket 2 241 is located at the lower end inside protection liquid storehouse 23, and 2 242 upper end of pushing ram is fixedly connected with gasket 2 241, Strip groove 243 is located at the right side of gear 2 25, and rack rails 2 245 is located inside strip groove 243 vertically, and sheet metal 244 is fixed vertically In the right side inner wall of strip groove 243, as shown in figure 4, passing through 244 lower end of sheet metal and rack rails on the left of the lower end of pushing ram 2 242 2 245 lower ends are fixedly connected, and the discharge rate of protection liquid is accurately controlled by gear two, and support is connected 2 front ends by rotating bar 15 There is gear 2 25,24 engaged transmission of gear 2 25 and protection liquid storehouse propulsion device, 3 front end of hose is laterally set in syringe needle 13 Portion is wrapped on wheel disc 17 in the middle part of hose 3, and 3 end of hose is connect with hollow shaft 4, passes through pipe between embryo storehouse 21 and hollow shaft 4 Road 1 communicates, and protects and is communicated between liquid storehouse 23 and pipeline 1 by pipeline 26.
The working method of the present embodiment:
S1: determining injection position by inspection equipment, embryo and its nutrient solution be injected into embryo storehouse by syringe, will Protection liquid is injected into inside protection liquid storehouse, and is matched granary by pushing ram one and the discharge of pushing ram two and protected the air in liquid storehouse, Then fixed disk is adjacent at the skin of parent injection position.
S2: driving rack rails one to move upwards by rotate gear two, so that pushing ram two be driven to move upwards, protection liquid from Pipeline two is introduced into pipeline one, then reaches hose front end, when liquid to be protected is overflowed from needle position, rotate gear one is by syringe needle It is pierced into inside maternal uterine, when syringe needle is pierced into maternal uterine, rotating wheel disk makes inside the extended needle of hose head, pushes to promote and press After embryo storehouse internal liquid is discharged in button, rotate gear two makes after protecting liquid storehouse remaining liq to be all discharged again, removes the present apparatus.
The above described is only a preferred embodiment of the present invention, being not the limitation for making other forms to the present invention, appoint What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc. Imitate embodiment.But without departing from the technical solutions of the present invention, according to the technical essence of the invention to above embodiments institute Any simple modification, equivalent variations and the remodeling made, still fall within the protection scope of technical solution of the present invention.

Claims (10)

1. a kind of embryo transplanter for test-tube baby, which is characterized in that including fixed link (1), support (2), hose (3), Hollow shaft (4), pipeline one (5), pipeline two (6), fixed link (1) front end are connected with fixed disk (11) by card slot, fixed It is transversely provided in the middle part of bar (1) channel (12), is equipped with syringe needle (13) inside the channel (12), on the downside of syringe needle (13) rear end Equipped with rack rails one (14), lower end is rotatably connected to gear one (16), the tooth by rotating bar (15) in the middle part of the fixed link (1) One (16) and rack rails one (14) engaged transmission are taken turns, fixed link (1) rear end is rotatably connected to wheel disc by the hollow shaft (4) (17), the support is connected to (2) below the rear end of fixed link (1), and support sets the rear side upper end of (2) embryo storehouse (21), described It is connected with embryo storehouse propulsion device (22) below embryo storehouse (21), protection liquid storehouse is fixed with below the embryo storehouse (21) (23), protection liquid storehouse (23) lower end is connected with protection liquid storehouse propulsion device (24), and (2) front end is passed through rotating bar (15) by support It is connected with gear two (25), gear two (25) and protection liquid storehouse propulsion device (24) engaged transmission, hose (3) front end are lateral It is internal to be set on syringe needle (13), is wrapped on wheel disc (17) in the middle part of hose (3), hose (3) end is connect with hollow shaft (4), embryo It is communicated between tire storehouse (21) and hollow shaft (4) by the pipeline one (5), between the protection liquid storehouse (23) and pipeline one (5) It is communicated by the pipeline two (6).
2. a kind of embryo transplanter for test-tube baby as described in claim 1, which is characterized in that the fixed disk (11) Including disk body (111), centre bore (112), sucker (113), connecting column (114), the centre bore (112) is located at the disk body (111) center, there are four the connecting columns (114), and four connecting columns (114) are uniformly distributed circumferentially before disk body (111) End, there are four the suckers (113), and four suckers (113) are separately fixed at the front end of connecting column (114).
3. a kind of embryo transplanter for test-tube baby as described in claim 1, which is characterized in that in the wheel disc (17) The heart is fixedly connected with hollow shaft (4), and the left end of hollow shaft (4) is rotatablely connected by card slot and pipeline one (5), hollow shaft (4) Right end is equipped with limited post (41), and the right end of the limited post (41) is fixedly connected with the inner wall of fixed link (1), limited post (41) Left end is for limiting hollow shaft (4).
4. a kind of embryo transplanter for test-tube baby as described in claim 1, which is characterized in that the embryo storehouse promotes Device (22) includes gasket one (221), pushing ram one (222), promotes button (223), and the gasket one (221) is located at embryo The internal lower end in tire storehouse (21), pushing ram one (222) upper end are fixedly connected with gasket one (221), pushing ram one (222) Lower end passes through support and (2) outer wall is fixedly connected with the propulsion button (223).
5. a kind of embryo transplanter for test-tube baby as described in claim 1, which is characterized in that the protection liquid storehouse pushes away It include gasket two (241), pushing ram two (242), strip groove (243), sheet metal (244), rack rails two into device (24) (245), the gasket two (241) is located at the internal lower end of protection liquid storehouse (23), pushing ram two (242) upper end and sealing Pad two (241) is fixedly connected, and the strip groove (243) is located at the right side of gear two (25), and the rack rails two (245) is located at vertically Strip groove (243) is internal, and the sheet metal (244) is fixed on the right side inner wall of strip groove (243), pushing ram two (242) vertically Lower end on the left of pass through sheet metal (244) lower end be fixedly connected with rack rails two (245) lower end.
6. a kind of embryo transplanter for test-tube baby as described in claim 1, which is characterized in that the embryo storehouse (21) For nutrient solution needed for storing embryo and embryonic development, the nutrient solution is by following weight percent at being grouped as: 0.02% penicillin, compound amido acid injection 5%, human serum albumin 6.3%, potassium chloride 1.2%, magnesium sulfate 0.5%, grape Sugared 6%, gentamicin 0.08%, ethylenediamine tetra-acetic acid 3.2%, Sodium Pyruvate 2.2%, alanyl glutamine 1.5%, ox sulphur Acid 0.85%, D-glucitol 1.2%, calcium chloride 0.9%, sodium dihydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.7%, sodium carbonate 5%, Lipoic acid 6%, n-acetyl-L-cysteine 3.2%, surplus are water.
7. a kind of embryo transplanter for test-tube baby as described in claim 1, which is characterized in that the protection liquid storehouse (23) internal injection has protection liquid, and the protection liquid is by following weight percent at being grouped as: creatinine 0.84%, vernix caseosa sub- oil Sour 0.54%, beta carotene 0.5%, palmitinic acid 0.8%, L-Glutamine 0.45%, polylactic-co-glycolic acid 0.32%, beta -mercaptoethanol 0.16%, human serum albumin 2.3%, vitamin B1 .2%, epidermal growth factor 0.02% and pancreas Island element 0.12%, hydrocortisone 0.23%, cortisol 0.04%, progesterone 0.94%, L-Glutamine 0.74%, HEPES0.84%.
8. a kind of embryo transplanter for test-tube baby as claimed in claim 6, which is characterized in that the nutrient solution sum PH is 6.9-7.2, osmotic pressure 312-322KPa.
9. a kind of embryo transplanter for test-tube baby as claimed in claim 6, which is characterized in that the nutrient solution sum PH is 6.9-7.2, osmotic pressure 312-322KPa.
10. a kind of embryo transplanter for test-tube baby as claimed in claim 7, which is characterized in that the protection liquid PH is 6.9-7.2, osmotic pressure 312-322KPa.
CN201811331453.7A 2018-11-09 2018-11-09 Embryo transplanter for test-tube baby Expired - Fee Related CN109223143B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111529025A (en) * 2020-05-25 2020-08-14 河南省人民医院 Test tube infant transplanting device and embryo transplanting method

Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0071538A1 (en) * 1981-07-31 1983-02-09 Bertrand Cassou Apparatus for transferring animal reproduction elements, embryos or the like
JPS6297545A (en) * 1985-10-24 1987-05-07 オリンパス光学工業株式会社 Transplantation needle
US4731052A (en) * 1987-01-14 1988-03-15 Seitz Jr H Michael Method for removing tissue and living organisms
CN1110469A (en) * 1993-06-04 1995-10-18 (株)科学畜产 Artificial insemination and embryo transfer device
CN1452473A (en) * 1997-05-09 2003-10-29 比奥费泰克有限公司 Container assembly for fertilization and culture and embryo transfer and method for fertilization and culture employing such container
EP1358856A1 (en) * 2002-04-29 2003-11-05 Universita Degli Studi Di Siena Device for intrauterine, intrabutal and intraperitoneal transfer of biological material
GB2399290A (en) * 2002-09-19 2004-09-15 Smiths Group Plc Oocyte recovery apparatus
CN201658407U (en) * 2010-04-21 2010-12-01 马博 Embryo transfer device
CN203898495U (en) * 2014-03-31 2014-10-29 中国医学科学院医学生物学研究所 Embryo transfer device for rat and mouse embryo transfer
CN204744353U (en) * 2015-02-13 2015-11-11 浙江大学医学院附属妇产科医院 Directional embryo transfer ware in uterus
CN204765845U (en) * 2015-06-06 2015-11-18 刘金勇 Embryo transfer pipe
WO2016101226A1 (en) * 2014-12-25 2016-06-30 河北农业大学 Embryo transplantation device and operation method thereof
CN106344132A (en) * 2016-10-09 2017-01-25 上海市第妇婴保健院 Embryo transplantation tube
CN206508074U (en) * 2016-11-21 2017-09-22 青岛松良基因科技有限公司 A kind of embryo transfer device component and embryo transfer device
CN107485464A (en) * 2017-09-13 2017-12-19 马博 A kind of embryo transplanter
CN207286179U (en) * 2017-02-17 2018-05-01 中国农业科学院特产研究所 A kind of embryo transplanter and automation embryo transplanter

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0071538A1 (en) * 1981-07-31 1983-02-09 Bertrand Cassou Apparatus for transferring animal reproduction elements, embryos or the like
JPS6297545A (en) * 1985-10-24 1987-05-07 オリンパス光学工業株式会社 Transplantation needle
US4731052A (en) * 1987-01-14 1988-03-15 Seitz Jr H Michael Method for removing tissue and living organisms
CN1110469A (en) * 1993-06-04 1995-10-18 (株)科学畜产 Artificial insemination and embryo transfer device
CN1452473A (en) * 1997-05-09 2003-10-29 比奥费泰克有限公司 Container assembly for fertilization and culture and embryo transfer and method for fertilization and culture employing such container
EP1358856A1 (en) * 2002-04-29 2003-11-05 Universita Degli Studi Di Siena Device for intrauterine, intrabutal and intraperitoneal transfer of biological material
GB2399290A (en) * 2002-09-19 2004-09-15 Smiths Group Plc Oocyte recovery apparatus
CN201658407U (en) * 2010-04-21 2010-12-01 马博 Embryo transfer device
CN203898495U (en) * 2014-03-31 2014-10-29 中国医学科学院医学生物学研究所 Embryo transfer device for rat and mouse embryo transfer
WO2016101226A1 (en) * 2014-12-25 2016-06-30 河北农业大学 Embryo transplantation device and operation method thereof
CN204744353U (en) * 2015-02-13 2015-11-11 浙江大学医学院附属妇产科医院 Directional embryo transfer ware in uterus
CN204765845U (en) * 2015-06-06 2015-11-18 刘金勇 Embryo transfer pipe
CN106344132A (en) * 2016-10-09 2017-01-25 上海市第妇婴保健院 Embryo transplantation tube
CN206508074U (en) * 2016-11-21 2017-09-22 青岛松良基因科技有限公司 A kind of embryo transfer device component and embryo transfer device
CN207286179U (en) * 2017-02-17 2018-05-01 中国农业科学院特产研究所 A kind of embryo transplanter and automation embryo transplanter
CN107485464A (en) * 2017-09-13 2017-12-19 马博 A kind of embryo transplanter

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111529025A (en) * 2020-05-25 2020-08-14 河南省人民医院 Test tube infant transplanting device and embryo transplanting method

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