CN109223143B - Embryo transplanter for test-tube baby - Google Patents

Embryo transplanter for test-tube baby Download PDF

Info

Publication number
CN109223143B
CN109223143B CN201811331453.7A CN201811331453A CN109223143B CN 109223143 B CN109223143 B CN 109223143B CN 201811331453 A CN201811331453 A CN 201811331453A CN 109223143 B CN109223143 B CN 109223143B
Authority
CN
China
Prior art keywords
embryo
rod
pipeline
hollow shaft
test
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201811331453.7A
Other languages
Chinese (zh)
Other versions
CN109223143A (en
Inventor
王爱华
李玉新
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wang Aihua
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201811331453.7A priority Critical patent/CN109223143B/en
Publication of CN109223143A publication Critical patent/CN109223143A/en
Application granted granted Critical
Publication of CN109223143B publication Critical patent/CN109223143B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/42—Gynaecological or obstetrical instruments or methods
    • A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
    • A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/16—Magnesium; Mg chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Surgery (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Pregnancy & Childbirth (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Medical Informatics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses an embryo transplanter for test-tube infants, which relates to the technical field of gynecological family planning and comprises a fixed rod, a holding handle, a hose, a hollow shaft, a first pipeline and a second pipeline; the rotating gear II drives the rack I to move upwards so as to drive the pushing rod to move upwards, the protective liquid enters the pipeline I from the pipeline II and then reaches the front end of the hose, when the protective liquid overflows from the position of the needle head, the rotating gear I enables the needle head to penetrate into the uterus of the mother body, when the needle head penetrates into the uterus of the mother body, the rotating wheel disc enables the head of the hose to extend out of the needle head, after the pushing button is pushed to discharge the liquid in the embryo bin, the gear II is rotated again so as to discharge all the residual liquid in the protective liquid bin, and the device is taken down.

Description

Embryo transplanter for test-tube baby
Technical Field
The invention relates to the technical field of gynecological family planning, in particular to an embryo transplanter for test-tube infants.
Background art:
in China, in vitro fertilization and embryo transfer (IVF-ET) is often called as a test-tube infant. In fact, in vitro fertilization is a special technique in which both the ovum and the sperm are brought outside the body, allowed to complete the fertilization process in an in vitro manually controlled environment, and then the early embryo is transferred into the uterus of a female where it is gestated into a child. Infants produced using in vitro fertilization techniques are called tube infants, and these infants also grow in the uterus of mothers. It can be said that "tube infant technique" is equivalent to "in vitro fertilization.
Among the treatments for infertility diseases, tube infants are one of the common effective treatments. However, in practice, the embryo transfer is not performed properly, so that the cultured embryo cannot be successfully transferred into the uterus, which results in failure. Especially for those mothers who suffer from cervical canal deformity, it is more difficult to deliver embryos into the uterine cavity through the deformed cervical canal, and the selection of the healthiest embryo for injection into the mother's uterus for conception is not easy, so the safety of the embryo during injection is important, but the current embryo injection tools are too simple and have lower safety factors.
Disclosure of Invention
In view of the above technical problems, the present invention provides an embryo transfer device for test-tube infants.
The technical scheme of the invention is as follows:
an embryo transplanter for test-tube babies comprises a fixed rod, a support handle, a hose, a hollow shaft, a first pipeline and a second pipeline, wherein the front end of the fixed rod is connected with a fixed disc through a clamping groove, the middle part of the fixed rod is transversely provided with a channel, a needle head is arranged in the channel, a first toothed rail is arranged on the lower side of the rear end of the needle head, the lower end of the middle part of the fixed rod is rotatably connected with a first gear through the rotary rod, the first gear is in meshing transmission with the first toothed rail, the rear end of the fixed rod is rotatably connected with a wheel disc through the hollow shaft, the support handle is connected below the rear end of the fixed rod, an embryo bin is arranged at the upper end of the rear side of the support handle, an embryo bin propelling device is connected below the embryo bin, a protection liquid bin is fixed below the embryo bin, the lower end of the protection liquid bin is connected with a protection liquid bin, the front end of the hose transversely penetrates through the needle head, the middle of the hose is wound on the wheel disc, the tail end of the hose is connected with the hollow shaft, the embryo chamber is communicated with the hollow shaft through the first pipeline, and the protection liquid chamber is communicated with the first pipeline through the second pipeline.
Further, the fixed disk includes disk body, centre bore, sucking disc, spliced pole, the centre bore is located the center of disk body, the spliced pole has four, and four spliced poles are at the disk body front end along circumference evenly distributed, the sucking disc has four, and four sucking discs are fixed respectively at the front end of spliced pole, reduce the internal removal of syringe needle at the parent through the fixed disk.
Further, rim plate center and hollow shaft fixed connection, the left end of hollow shaft passes through draw-in groove and a rotation connection of pipeline, and the right-hand member of hollow shaft is equipped with spacing post, the right-hand member of spacing post and the inner wall fixed connection of dead lever, the left end of spacing post is used for spacing the hollow shaft, stretches out the hose inside the syringe needle through the rim plate, avoids the syringe needle to embryo scratch.
Further, embryo storehouse advancing device is including sealed one, pushing ram one, push button, sealed one is located the inside lower extreme in embryo storehouse, a pushing ram upper end and sealed one fixed connection that fills up, a pushing ram lower extreme pass the support outer wall with push button fixed connection, through the inside liquid of push button quick discharge embryo storehouse.
Further, protection fluid storehouse advancing device is including sealed two, pushing ram two, bar groove, sheetmetal, cogged rail two sealed pad is two in the inside lower extreme of protection fluid storehouse, two upper ends of pushing ram and sealed two fixed connection of pad, the bar groove is located the right side of gear two, two vertical establishments of cogged rail are inside the bar inslot, the vertical right side inner wall of fixing in the bar groove of sheetmetal, the lower extreme left side of pushing ram two passes sheetmetal lower extreme and two lower extreme fixed connection of cogged rail, through the discharge amount of two accurate control protection fluids of gear.
Further, the embryo storehouse is used for storing embryo and the required nutrient solution of embryo development, the nutrient solution comprises the following components by weight percent: 0.02% penicillin, 5% compound amino acid injection, 6.3% human serum albumin, 1.2% potassium chloride, 0.5% magnesium sulfate, 6% glucose, 0.08% gentamicin, 3.2% ethylene diamine tetraacetic acid, 2.2% sodium pyruvate, 1.5% alanyl glutamine, 0.85% taurine, 1.2% D-sorbitol, 0.9% calcium chloride, 0.5% sodium dihydrogen phosphate, 0.7% potassium dihydrogen phosphate, 5% sodium carbonate, 6% lipoic acid, 3.2% N-acetyl-L-cysteine, and the balance water, thereby enhancing the embryo activity and enhancing the conception probability of the mother body.
Further, a protection solution is injected into the protection solution bin, and the protection solution comprises the following components in percentage by weight: 0.84% of creatinine, 0.54% of linoleic acid, 0.5% of beta-carotene, 0.8% of palmitic acid, 0.45% of L-glutamine, 0.32% of polylactic acid-glycolic acid copolymer, 0.16% of beta-mercaptoethanol, 2.3% of human serum albumin, 1.2% of vitamin B, 0.02% of epidermal growth factor, 0.12% of insulin and 0.23% of hydrocortisone; testosterone 0.04%, progesterone 0.94%, L-glutamine 0.74%, HEPES 0.84%, and protective solution for stimulating mother body to perform pregnancy reaction.
Further, the pH value of the nutrient solution is 6.9-7.2, and the osmotic pressure is 312-322KPa, so that the healthy development of the embryo is ensured.
Further, the pH value of the protective solution is 6.9-7.2, and the osmotic pressure is 312-322KPa, so that the healthy development of the embryo is ensured.
The working method of the invention comprises the following steps:
s1: the injection position is determined through the inspection equipment, the embryo and the nutrient solution thereof are injected into the embryo bin through the injector, the protective solution is injected into the protective solution bin, the air in the embryo bin and the air in the protective solution bin are exhausted through the first push rod and the second push rod, and then the fixed disc is attached to the skin of the mother injection position.
S2: drive rack one upward movement through rotating gear two to drive the pushing ram and move up, protection liquid enters pipeline one from pipeline two first, then reachs the hose front end, when waiting that protection liquid to spill over from the syringe needle position, rotating gear one pierces the syringe needle inside the mother uterus, when the syringe needle pierces the mother uterus, it makes the hose head stretch out inside the syringe needle to rotate the rim plate, promote behind the inside liquid in push button discharge embryo storehouse, after rotating gear two again makes the whole dischargings of protection liquid storehouse surplus liquid, take off this device.
The invention has the beneficial effects that:
the invention pushes nutrient solution containing embryo to advance to mother uterus through protective solution, after the needle head enters the uterus, the head of the hose extends out of the interior of the needle head through the rotating wheel disc, thus when the needle head is pulled out, the needle head is prevented from scratching embryo cells to influence the health condition of the embryo, the fixed disc can stabilize the device, and the needle head is prevented from generating large-amplitude displacement in the mother body, thereby scratching the inner wall of the uterus.
Drawings
FIG. 1 is a block diagram of the invention;
FIG. 2 is a block diagram of the wheel disc of the present invention;
FIG. 3 is a left side view of the inventive stationary disk;
fig. 4 is a connection diagram of the metal sheet and the second push rod of the invention.
Wherein, 1-a fixed rod, 2-a holding handle, 3-a hose, 4-a hollow shaft, 5-a first pipeline, 6-a second pipeline, 11-a fixed disk, 12-a channel, 13-a needle, 14-a rack rail, 15-a rotating rod, 16-a first gear, 17-a wheel disc, 21-an embryo chamber, 22-an embryo chamber propelling device and 23-a protective liquid chamber, 24-protection liquid bin propelling device, 25-gear II, 111-disc body, 112-center hole, 113-sucker, 114-connecting column, 41-limiting column, 221-sealing gasket I, 222-propelling rod I, 223-propelling button, 241-sealing gasket II, 242-propelling rod II, 243-strip groove, 244-metal sheet and 245-toothed rail II.
Detailed Description
For the convenience of understanding the technical solution of the present invention, the following is further explained with reference to fig. 1-2, and the embodiments do not limit the scope of the present invention.
Example 1:
as shown in figure 1, the embryo transplanter for the test tube babies comprises a fixed rod 1, a support handle 2, a hose 3, a hollow shaft 4, a first pipeline 5 and a second pipeline 6, wherein the front end of the fixed rod 1 is connected with a fixed disk 11 through a clamping groove, as shown in figure 3, the fixed disk 11 comprises a disk body 111, a central hole 112, suckers 113 and connecting columns 114, the central hole 112 is positioned in the center of the disk body 111, the four connecting columns 114 are uniformly distributed at the front end of the disk body 111 along the circumferential direction, the suckers 113 are four, the four suckers 113 are respectively fixed at the front end of the connecting columns 114, the movement of a needle head in a mother body is reduced through the fixed disk, a channel 12 is transversely arranged in the middle of the fixed rod 1, a needle head 13 is arranged in the channel 12, a first rack 14 is arranged on the lower side of the rear end of the needle head 13, a first, the rear end of the fixed rod 1 is rotatably connected with a wheel disc 17 through a hollow shaft 4, as shown in fig. 2, the center of the wheel disc 17 is fixedly connected with the hollow shaft 4, the left end of the hollow shaft 4 is rotatably connected with a first pipeline 5 through a clamping groove, the right end of the hollow shaft 4 is provided with a limiting column 41, the right end of the limiting column 41 is fixedly connected with the inner wall of the fixed rod 1, the left end of the limiting column 41 is used for limiting the hollow shaft 4, a hose extends out of the inside of a needle through the wheel disc to avoid the needle from scratching embryos, the holder 2 is connected below the rear end of the fixed rod 1, the upper end of the rear side of the holder 2 is provided with an embryo chamber 21, the lower end of the embryo chamber 21 is connected with an embryo chamber propelling device 22, the embryo chamber propelling device 22 comprises a first sealing gasket 221, a first propelling rod 222 and a propelling button 223, the first sealing gasket 221 is positioned at the lower end of the inside of the embryo chamber 21, liquid in the embryo bin is quickly discharged through a push button, a protective liquid bin 23 is fixed below the embryo bin 21, the embryo bin 21 is used for storing embryos and nutrient liquid required by embryo development, the activity of the embryos is enhanced through the nutrient liquid, so that the pregnancy probability of a mother body is enhanced, the protective liquid is injected into the protective liquid bin 23 and stimulates the mother body to perform pregnancy reaction, the pH value of the protective liquid is 6.9-7.2, the osmotic pressure is 312-322KPa, the pH value of the nutrient liquid is 6.9-7.2, the osmotic pressure is 312-322KPa, the healthy development of the embryos is ensured, the lower end of the protective liquid bin 23 is connected with a protective liquid bin propelling device 24, the protective liquid bin propelling device 24 comprises a second sealing gasket 241, a second push rod 242, a strip-shaped groove 243, a metal sheet 244, a second rack 245 and a second sealing gasket 241, the upper end of the second push rod 242 is fixedly connected with the second sealing gasket 241, the strip-shaped groove 243 is located on the right side of the second gear 25, the second rack 245 is vertically arranged inside the strip-shaped groove 243, the metal sheet 244 is vertically fixed on the inner wall of the right side of the strip-shaped groove 243, as shown in fig. 4, the left side of the lower end of the second push rod 242 penetrates through the lower end of the metal sheet 244 and is fixedly connected with the lower end of the second rack 245, the discharge amount of the protective liquid is accurately controlled through the second gear, the front end of the carrying handle 2 is connected with the second gear 25 through the rotating rod 15, the second gear 25 is in meshing transmission with the protective liquid bin pushing device 24, the front end of the hose 3 is transversely sleeved inside the needle 13 in a penetrating mode, the middle of the hose 3 is wound on the wheel disc 17, the tail end of the hose 3 is connected with the hollow shaft 4, the.
Example 2:
as shown in figure 1, the embryo transplanter for the test tube babies comprises a fixed rod 1, a support handle 2, a hose 3, a hollow shaft 4, a first pipeline 5 and a second pipeline 6, wherein the front end of the fixed rod 1 is connected with a fixed disk 11 through a clamping groove, as shown in figure 3, the fixed disk 11 comprises a disk body 111, a central hole 112, suckers 113 and connecting columns 114, the central hole 112 is positioned in the center of the disk body 111, the four connecting columns 114 are uniformly distributed at the front end of the disk body 111 along the circumferential direction, the suckers 113 are four, the four suckers 113 are respectively fixed at the front end of the connecting columns 114, the movement of a needle head in a mother body is reduced through the fixed disk, a channel 12 is transversely arranged in the middle of the fixed rod 1, a needle head 13 is arranged in the channel 12, a first rack 14 is arranged on the lower side of the rear end of the needle head 13, a first, the rear end of the fixed rod 1 is rotatably connected with a wheel disc 17 through a hollow shaft 4, as shown in fig. 2, the center of the wheel disc 17 is fixedly connected with the hollow shaft 4, the left end of the hollow shaft 4 is rotatably connected with a first pipeline 5 through a clamping groove, the right end of the hollow shaft 4 is provided with a limiting column 41, the right end of the limiting column 41 is fixedly connected with the inner wall of the fixed rod 1, the left end of the limiting column 41 is used for limiting the hollow shaft 4, a hose extends out of the inside of a needle through the wheel disc to avoid the needle from scratching embryos, the holder 2 is connected below the rear end of the fixed rod 1, the upper end of the rear side of the holder 2 is provided with an embryo chamber 21, the lower end of the embryo chamber 21 is connected with an embryo chamber propelling device 22, the embryo chamber propelling device 22 comprises a first sealing gasket 221, a first propelling rod 222 and a propelling button 223, the first sealing gasket 221 is positioned at the lower end of the inside of the embryo chamber 21, the liquid in the embryo storehouse is discharged rapidly by pushing the button, the protection liquid storehouse 23 is fixed below the embryo storehouse 21, the embryo storehouse 21 is used for storing embryos and nutrient solution required by embryo development, and the nutrient solution consists of the following components in percentage by weight: 0.02% of penicillin, 5% of compound amino acid injection, 6.3% of human serum albumin, 1.2% of potassium chloride, 0.5% of magnesium sulfate, 6% of glucose, 0.08% of gentamicin, 3.2% of ethylenediamine tetraacetic acid, 2.2% of sodium pyruvate, 1.5% of alanylglutamine, 0.85% of taurine, 1.2% of D-sorbitol, 0.9% of calcium chloride, 0.5% of sodium dihydrogen phosphate, 0.7% of potassium dihydrogen phosphate, 5% of sodium carbonate, 6% of lipoic acid, 3.2% of N-acetyl-L-cysteine and the balance of water, so that the embryo activity is enhanced, the pregnancy probability of a mother body is enhanced, and a protective solution is injected into the protective solution bin 23 and consists of the following components in percentage by weight: 0.84% of creatinine, 0.54% of vernix caseosa linoleic acid, 0.5% of beta-carotene, 0.8% of palmitic acid, 0.45% of L-glutamine, 0.32% of polylactic acid-glycolic acid copolymer, 0.16% of beta-mercaptoethanol, 2.3% of human serum albumin L, 1.2% of vitamin B, 0.02% of epidermal growth factor, 0.12% of insulin, 0.23% of hydrocortisone, 0.04% of testosterone, 0.94% of progesterone, 0.74% of L-glutamine and 0.84% of HEPES (high performance liquid), stimulating the parent through a protective solution to perform a pregnancy reaction, wherein the pH of the protective solution is 6.9-7.2, the osmotic pressure is 312-322KPa, the pH of the nutrient solution and the pH of the nutrient solution are 6.9-7.2, the osmotic pressure is 312-322KPa, the healthy development of the embryo is ensured, the lower end of the protective solution cabin 23 is connected with a protective solution cabin propelling device 24, and the protective solution cabin propelling device, The strip-shaped groove 243, the metal sheet 244, the second toothed rail 245 and the second sealing gasket 241 are positioned at the lower end inside the protective liquid bin 23, the upper end of the second pushing rod 242 is fixedly connected with the second sealing gasket 241, the strip-shaped groove 243 is positioned at the right side of the second gear 25, the second toothed rail 245 is vertically arranged inside the strip-shaped groove 243, the metal sheet 244 is vertically fixed on the inner wall of the right side of the strip-shaped groove 243, as shown in fig. 4, the left side of the lower end of the second push rod 242 passes through the lower end of the metal sheet 244 and is fixedly connected with the lower end of the second rack 245, the discharge amount of the protective liquid is accurately controlled through the second gear, the front end of the carrying handle 2 is connected with the second gear 25 through the rotating rod 15, the second gear 25 is in meshing transmission with the protective liquid bin propelling device 24, the front end of the hose 3 transversely penetrates through the needle head 13, the middle of the hose 3 is wound on the wheel disc 17, the tail end of the hose 3 is connected with the hollow shaft 4, the embryo bin 21 is communicated with the hollow shaft 4 through the first pipeline 5, and the protective liquid bin 23 is communicated with the first pipeline 5 through the second pipeline 6.
The working method of the embodiment comprises the following steps:
s1: the injection position is determined through the inspection equipment, the embryo and the nutrient solution thereof are injected into the embryo bin through the injector, the protective solution is injected into the protective solution bin, the air for matching the Tai bin and the protective solution bin is discharged through the first push rod and the second push rod, and then the fixed disc is attached to the skin of the mother injection position.
S2: drive rack one upward movement through rotating gear two to drive the pushing ram and move up, protection liquid enters pipeline one from pipeline two first, then reachs the hose front end, when waiting that protection liquid to spill over from the syringe needle position, rotating gear one pierces the syringe needle inside the mother uterus, when the syringe needle pierces the mother uterus, it makes the hose head stretch out inside the syringe needle to rotate the rim plate, promote behind the inside liquid in push button discharge embryo storehouse, after rotating gear two again makes the whole dischargings of protection liquid storehouse surplus liquid, take off this device.
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.

Claims (9)

1. The embryo transplanter for the test tube babies is characterized by comprising a fixed rod (1), a holder (2), a hose (3), a hollow shaft (4), a first pipeline (5) and a second pipeline (6), wherein the front end of the fixed rod (1) is connected with a fixed disk (11) through a clamping groove, the middle part of the fixed rod (1) is transversely provided with a channel (12), a needle (13) is arranged inside the channel (12), a first rack (14) is arranged on the lower side of the rear end of the needle (13), a first gear (16) is rotatably connected to the lower end of the middle part of the fixed rod (1) through a rotating rod (15), the first gear (16) is in meshing transmission with the first rack (14), the rear end of the fixed rod (1) is rotatably connected with a wheel disc (17) through the hollow shaft (4), the holder (2) is connected below the rear end of the fixed rod (1), and an embryo bin (21) is arranged at the upper, embryo storehouse (21) below is connected with embryo storehouse advancing device (22), the below in embryo storehouse (21) is fixed with protection liquid storehouse (23), protection liquid storehouse (23) lower extreme is connected with protection liquid storehouse advancing device (24), holds in the palm and is connected with two (25) gears (2) front end through dwang (15), two (25) gears with protection liquid storehouse advancing device (24) meshing transmission, hose (3) front end transversely wears to overlap inside syringe needle (13), and hose (3) middle part winding is on rim plate (17), and hose (3) end is connected with hollow shaft (4), passes through between embryo storehouse (21) and hollow shaft (4) pipeline (5) communicate with each other, pass through between protection liquid storehouse (23) and the pipeline (5) pipeline two (6) communicate with each other.
2. The embryo transfer device for the test-tube baby as claimed in claim 1, wherein the fixed tray (11) comprises a tray body (111), a central hole (112), four connecting columns (114), four sucking discs (113) and four connecting columns (114), the central hole (112) is located at the center of the tray body (111), the four connecting columns (114) are evenly distributed at the front end of the tray body (111) along the circumferential direction, the four sucking discs (113) are arranged, and the four sucking discs (113) are respectively fixed at the front ends of the connecting columns (114).
3. The embryo transfer device for test-tube babies, according to claim 1, wherein the center of the wheel disc (17) is fixedly connected with the hollow shaft (4), the left end of the hollow shaft (4) is rotatably connected with the first pipeline (5) through a clamping groove, the right end of the hollow shaft (4) is provided with a limiting column (41), the right end of the limiting column (41) is fixedly connected with the inner wall of the fixing rod (1), and the left end of the limiting column (41) is used for limiting the hollow shaft (4).
4. The embryo transfer device for test-tube babies as claimed in claim 1, wherein the embryo chamber propelling device (22) comprises a first sealing gasket (221), a first propelling rod (222) and a propelling button (223), the first sealing gasket (221) is arranged at the lower end inside the embryo chamber (21), the upper end of the first propelling rod (222) is fixedly connected with the first sealing gasket (221), and the lower end of the first propelling rod (222) penetrates through the outer wall of the holder (2) and is fixedly connected with the propelling button (223).
5. The embryo transfer device for the test-tube baby as claimed in claim 1, wherein the protective solution chamber propulsion unit (24) comprises a second sealing gasket (241), a second propulsion rod (242), a strip-shaped groove (243), a metal sheet (244) and a second toothed rail (245), the second sealing gasket (241) is located at the lower end inside the protective solution chamber (23), the upper end of the second propulsion rod (242) is fixedly connected with the second sealing gasket (241), the strip-shaped groove (243) is located at the right side of the second gear (25), the second toothed rail (245) is vertically arranged inside the strip-shaped groove (243), the metal sheet (244) is vertically fixed on the inner wall of the right side of the strip-shaped groove (243), and the left side of the lower end of the second propulsion rod (242) penetrates through the lower end of the metal sheet (244) and is fixedly connected with the lower end of the second toothed rail (245).
6. The embryo transfer device for test-tube babies according to claim 1, wherein the embryo chamber (21) is used for storing embryos and nutrient solution required for embryo development, and the nutrient solution comprises the following components by weight percentage: 0.02% penicillin, 5% compound amino acid injection, 6.3% human serum albumin, 1.2% potassium chloride, 0.5% magnesium sulfate, 6% glucose, 0.08% gentamicin, 3.2% ethylene diamine tetraacetic acid, 2.2% sodium pyruvate, 1.5% alanylglutamine, 0.85% taurine, 1.2% D-sorbitol, 0.9% calcium chloride, 0.5% sodium dihydrogen phosphate, 0.7% potassium dihydrogen phosphate, 5% sodium carbonate, 6% lipoic acid, 3.2% N-acetyl-L-cysteine, and the balance of water.
7. The embryo transfer device for test-tube infants as claimed in claim 6, wherein the pH of the nutrient solution is 6.9-7.2, and the osmotic pressure is 312-322 KPa.
8. The embryo transfer device for test-tube infants according to claim 1, characterized in that the protection liquid chamber (23) is injected with a protection liquid, and the protection liquid comprises the following components by weight percentage: 0.84% of creatinine, 0.54% of vernix caseosa linoleic acid, 0.5% of beta-carotene, 0.8% of palmitic acid, 0.45% of L-glutamine, 0.32% of polylactic acid-glycolic acid copolymer, 0.16% of beta-mercaptoethanol, 2.3% of human serum albumin, 1.2% of vitamin B, 0.02% of epidermal growth factor, and 0.12% of insulin, 0.23% of hydrocortisone, 0.04% of testosterone, 0.94% of progesterone, 0.74% of L-glutamine, and 0.84% of HEPES.
9. The embryo transfer device for test-tube infants as claimed in claim 8, wherein the pH of the protective solution is 6.9-7.2, and the osmotic pressure is 312-322 KPa.
CN201811331453.7A 2018-11-09 2018-11-09 Embryo transplanter for test-tube baby Expired - Fee Related CN109223143B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811331453.7A CN109223143B (en) 2018-11-09 2018-11-09 Embryo transplanter for test-tube baby

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811331453.7A CN109223143B (en) 2018-11-09 2018-11-09 Embryo transplanter for test-tube baby

Publications (2)

Publication Number Publication Date
CN109223143A CN109223143A (en) 2019-01-18
CN109223143B true CN109223143B (en) 2021-04-16

Family

ID=65077733

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811331453.7A Expired - Fee Related CN109223143B (en) 2018-11-09 2018-11-09 Embryo transplanter for test-tube baby

Country Status (1)

Country Link
CN (1) CN109223143B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111529025B (en) * 2020-05-25 2021-04-23 河南省人民医院 Test tube infant transplanting device and embryo transplanting method

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2510393A1 (en) * 1981-07-31 1983-02-04 Bertrand Cassou APPARATUS FOR TRANSFERRING ANIMAL REPRODUCTIVE ELEMENTS, SUCH AS EMBRYOS
JPS6297545A (en) * 1985-10-24 1987-05-07 オリンパス光学工業株式会社 Transplantation needle
US4731052A (en) * 1987-01-14 1988-03-15 Seitz Jr H Michael Method for removing tissue and living organisms
BR9405395A (en) * 1993-06-04 1999-09-08 Kwahak Internacional Co Ltda Device for artificial insemination and embryo transfer.
US6050935A (en) * 1997-05-09 2000-04-18 Biofertec Container assembly for intravaginal fertilization and culture and embryo transfer and method of intravaginal fertilization and culture employing such a container
ITRM20020230A1 (en) * 2002-04-29 2003-10-29 Univ Siena DEVICE FOR INTRAUTERINE, INTRATUBARIC OR INTRAPERITONEAL TRANSFER OF ORGANIC MATERIAL.
GB0221744D0 (en) * 2002-09-19 2002-10-30 Smiths Group Plc Oocyte recovery apparatus an systems
CN201658407U (en) * 2010-04-21 2010-12-01 马博 Embryo transfer device
CN203898495U (en) * 2014-03-31 2014-10-29 中国医学科学院医学生物学研究所 Embryo transfer device for rat and mouse embryo transfer
WO2016101226A1 (en) * 2014-12-25 2016-06-30 河北农业大学 Embryo transplantation device and operation method thereof
CN204744353U (en) * 2015-02-13 2015-11-11 浙江大学医学院附属妇产科医院 Directional embryo transfer ware in uterus
CN204765845U (en) * 2015-06-06 2015-11-18 刘金勇 Embryo transfer pipe
CN106344132B (en) * 2016-10-09 2018-12-07 上海市第一妇婴保健院 A kind of embryo transplantation tube
CN206508074U (en) * 2016-11-21 2017-09-22 青岛松良基因科技有限公司 A kind of embryo transfer device component and embryo transfer device
CN207286179U (en) * 2017-02-17 2018-05-01 中国农业科学院特产研究所 A kind of embryo transplanter and automation embryo transplanter
CN107485464B (en) * 2017-09-13 2019-06-07 马博 A kind of embryo transplanter

Also Published As

Publication number Publication date
CN109223143A (en) 2019-01-18

Similar Documents

Publication Publication Date Title
CN109223143B (en) Embryo transplanter for test-tube baby
CN109497040A (en) A kind of human oocytes and embryo vitrifying freeze thawing device and its application method
RU2014150657A (en) APPLICATION OF N-ACETYL-5-METHOXYTRIPTAMINE OR ITS ANALOGUES TO ACTIVATE THE EMBRYON IMPLANTATION MECHANISM, AND ALSO THE RELATED COMPOSITIONS AND CULTURAL MEDIA
US5759513A (en) Apparatus and process for preparing radioisotope-labeled reagent
CN107384854A (en) The ripe optimization liquid of human oocytes endochylema
CN204520782U (en) A kind of ultrasound guidance amniocentesis device
US20180036461A1 (en) Device and method for handling human breast milk
CN216294684U (en) Traditional chinese medical science acupuncture branch of academic or vocational study assistor
CN108614098B (en) Kit for activating ICSI (intracytoplasmic sperm injection) post-unfertilized oocyte and application method
CN103637856A (en) Single-handed direct-pressing type continuous quantitative liquid sucking and discharging device
CN202821556U (en) Embryo transplantation device
CN203576692U (en) One-hand direct pressure continuous quantitative liquid sucking and draining device
CN206923569U (en) A kind of liquid nitrogen container pail and its system for being applied to frozen embryo or freezing egg mother cell
CN217772584U (en) Be used for horse pipe for embryo transfer
Joo et al. Comparison of mechanical artificial shrinkage methods in mouse blastocyst vitrification
CN212316111U (en) Line marking mechanism for bacteria-containing material
CN216726973U (en) Poultry animal doctor uses medicine dispensing device
Dimitriadis et al. Artificial intelligence assistance for the quality assessment of embryo vitrification, warming and transfers in the IVF laboratory
CN204468291U (en) A kind of nursing tray
CN218856890U (en) Workstation for medical science experiments
CN220568263U (en) Adjustable weighing device for weighing biological materials
CN211300094U (en) Novel gynaecology's cervical sample ware of dosing
CN117089455A (en) Microgravity culture safety environment box
CN211586071U (en) Nutrient solution preparation equipment
McGee Jr et al. A new capillary tube system for measuring the uptake and release of materials from cultured cells

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Wang Aihua

Inventor after: Li Yuxin

Inventor before: Li Yuxin

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20210331

Address after: 080 Jiankang Road, Sishui County, Jining City, Shandong Province 273200

Applicant after: Wang Aihua

Address before: South head of yunmenshan South Road, cultural industry park, Qingzhou City, Weifang City, Shandong Province

Applicant before: WEIFANG NURSING VOCATIONAL College

GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20210416

Termination date: 20211109