KR20170033950A

KR20170033950A - Gathering eggs for livestock and animals

Info

Publication number: KR20170033950A
Application number: KR1020150131650A
Authority: KR
Inventors: 조경만
Original assignee: 주식회사 지엠엠씨
Priority date: 2015-09-17
Filing date: 2015-09-17
Publication date: 2017-03-28

Abstract

The present invention relates to a livestock and animal harvesting apparatus capable of acquiring a large amount of eggs at a time without complicated gathering process, being operable by a practitioner, and shortening the picking time. The present invention relates to a probe having an ultrasonic sensor at a tip thereof and inserted into a uterus of a target livestock; A suction needle unit inserted into the probe so as to be able to move back and forth along the inside of the probe and to suck in the uterus or the embryo; A collecting container connected to the suction needle unit for collecting eggs or embryos collected from the suction needle unit; A vacuum pump for sucking an oocyte or a fertilized egg into the suction needle unit by forming a suction force inside the suction needle unit by forming a negative pressure inside the packed vessel; And an ultrasound imaging unit receiving and displaying an image captured by the ultrasonic sensor.

Description

[0001] Gathering eggs for livestock and animals [0002]

The present invention relates to a livestock and animal harvesting apparatus, and more particularly, to a livestock and animal harvesting apparatus capable of acquiring a large amount of eggs at one time without any complicated gathering process, And more particularly, to a charging device.

At present, domestic egg-laying farms have a significantly lower egg-laying rate and fertilization efficiency than foreign countries due to the lack of systematic egg-laying and embryo transfer program. In addition, for high-quality Korean beef, the production system is mainly composed of hydrogen, so that the quality of high-quality seeds is not only slow but also ineffective.

On the other hand, in case of production of high-ability Hanwoo, the hereditary ability evaluation is conducted mainly as a result of verifying the ability of the sow moth (high ability), that is, the bull, and the ability of the bull ow (pregnant cattle) In addition, there are cases in which the offspring do not have excellent traits because they are stopped to be evaluated. Therefore, Korean domestic cattle have not only a small number of proven traits and proven sheep but also very low use of them.

In addition, the conventional method of collecting oocytes from slaughtered livestock farms has a problem that the quality of the oocytes is lowered, the survival rate and the fertilization rate are low, the fertilized eggs are degraded, and the fertility rate is lowered.

Thus, conventional catheterization techniques for livestock have been used in surgical and non-surgical procedures. Among these, embryo harvesting has been performed using a 3-way catheter and a 2-way catheter as non-surgical procedures. Surgical procedures were performed by inserting a fertilized ovum and revealing a magnetic reproductive tract. After inserting a tube at the bulbous or bulbous isthmus junction, the perfusion fluid was flown and inserted into the site where the tubal canal and uterine tip were joined. There is a method of collecting the embryos that flowed into the perfusion fluid and a method of collecting the embryos by collecting the perfusion fluid from the bottom of the uterus after inserting the tube into the uterine tip.

Such a conventional catheterization method requires a laparotomy during the procedure, which is costly and takes a long time compared with the non-surgical procedure in the catheterization procedure. In addition, many procedures are needed to fertilize the embryo, and it is not only difficult for the practitioner to perform the procedure by himself, but also has the disadvantage that it is difficult to collect reproductive eggs repeatedly from one livestock. Due to these problems, for example, in case of cattle slaughter, most of them are carried out by non-surgical methods mostly at home and abroad

In the non-surgical procedure, there is an embryo transfer method using an improved two-way catheter in the embryo transfer method using a three-way catheter. However, since the procedure is complicated and the construction of the surgical instruments is too many, In order to reduce the damage of the uterus, it is necessary to compensate the target livestock used for the chick in order to flow the perfused liquid for about 1 hour or more. Therefore, stress on the livestock used for chicketing due to long- There has been a problem in that a problem occurs.

In order to solve the above problem, the present invention can acquire a large amount of oocyte at one time without complex completing procedure, can be performed by the practitioner alone, shortens the picking time and minimizes the stress to which the cattle is subjected during the picking operation The present invention has been made in view of the above problems.

In order to achieve the above-mentioned object, the present invention provides a probe for a catheter, comprising: a probe having an ultrasonic sensor at a distal end thereof; A suction needle unit inserted into the probe so as to be able to move back and forth along the inside of the probe and to suck in the uterus or the embryo; A collecting container connected to the suction needle unit for collecting eggs or embryos collected from the suction needle unit; A vacuum pump for sucking an oocyte or a fertilized egg into the suction needle unit by forming a suction force inside the suction needle unit by forming a negative pressure inside the packed vessel; And an ultrasound imaging unit for receiving and displaying an image captured by the ultrasound sensor.

Wherein the suction needle unit comprises: a medical tube connected to the filler container; A pipe into which the medical tube is inserted; A suction needle holder to which the medical tube and the pipe are detachably connected; And a suction needle connected to the suction needle holder to communicate with the medical tube through the suction needle holder.

The suction needle may be coated with a silicon layer on the outer periphery and inside of the tip portion. The suction needle may be formed to have a first angle (?) Of 15 to 16 degrees and a second angle (?) Of 56 to 57 degrees. The suction needle may be formed with a blast treatment portion on the outer periphery of the distal end portion.

The pipe may be formed in a round shape of a metal material.

The probe may include a guide holder detachably disposed inside the tip of the probe to guide the forward and backward movement of the suction needle.

The vacuum pump can be electrically connected to a foot switch that can turn on and off the driving of the vacuum pump by the operator pressing the foot.

As described above, in the present invention, the livestock and animal harvesting apparatus can collect a large amount of oocytes or embryos at a time without complicated gathering process, can be performed by the practitioner alone, and can shorten the picking time.

In addition, the present invention does not use the hormone for collecting eggs for livestock to be harvested, so that the health and improvement of livestock and the safety of the reproductive organs can be ensured, and eggs and fertilized eggs can be effectively harvested even from cows with difficulty in pregnancy.

According to the present invention, the shape of the pointed tip of the suction needle is formed at first and second angles suitable for sticking to the skin of the livestock, the outer and inner peripheries of the suction needle tip are coated with silicone to secure durability of the needle, It is possible to increase the survival rate of the oocyte or embryo when moving the oocyte or embryo through the suction needle.

Further, according to the present invention, blast treatment is applied to the tip of a suction needle to prevent the ultrasound image from being distorted or reflected accurately on the tip surface of a suction needle, which is a metal material, during the ultrasound imaging, .

In addition, since the present invention enables the fertilization of a large number of eggs and fertilized eggs compared with the prior art, it is possible to enhance the competitiveness and industrialization of the livestock farming farm by establishing a high capacity livestock farming and animal improvement system. In addition, in the case of domestic wooshu, high-yielded high-grade Korean livestock products can contribute to securing superior Hanwoo genetic resources and enhancing competitiveness.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing a picking apparatus for livestock and non-livestock animals according to an embodiment of the present invention.
2 and 3 are a side view and a plan view showing the tip of the suction needle shown in Fig.
4 is a cross-sectional view taken along the line IV-IV shown in FIG.
FIG. 5 is a view showing an ultrasound imaging unit and a photographing screen shown in FIG. 1. FIG.
6 is a view showing a state in which a probe is inserted into a genital unit of a suction needle unit and a target livestock.
7 is a view showing a state in which a suction needle unit is inserted into a probe inserted in a target livestock reproductive system so that the tip of the suction needle is arranged at a position adjacent to the palm and the preparation for the procedure is completed.
Fig. 8 is a schematic diagram of an OPU oocyte retrieval process by a practitioner for cattle surgery.
9 is a view showing a state in which an ovum is taken through a suction needle in a state in which the ovary of the subject livestock is lifted with a hand inserted into the rectum.
FIG. 10 is a view showing a state in which the embryo is collected through the suction needle in a state in which the distal end of the uterus of the target livestock is lifted with the hand inserted into the rectum.

The present invention will become more apparent by describing in detail preferred embodiments thereof with reference to the accompanying drawings. Also, for ease of understanding of the invention, the appended drawings are not drawn to scale, but the dimensions of some of the components may be exaggerated.

The present invention employs an ovum pick-up method (OPU: ovum pick-up) of livestock and non-livestock animals. Hereinafter, referring to Figs. 1 to 5, The configuration of the bulking device 1 for non-livestock animals will be described in detail.

FIG. 1 is a schematic view showing a picking apparatus for livestock and non-livestock animals according to an embodiment of the present invention, FIGS. 2 and 3 are a side view and a plan view showing the tip of the suction needle shown in FIG. 1, 3 is a sectional view taken along the line IV-IV in FIG. 3, and FIG. 5 is a view showing the ultrasound imaging unit and the imaging screen shown in FIG.

1, the filler device 1 of the present invention may include a probe 10, a suction needle unit 30, a filler container 50, a vacuum pump 70 and an ultrasound imaging unit 90 have.

The probe 10 is inserted into the uterus of the target livestock to take an ultrasound image and guides the suction needle unit 30 into the uterus of the target livestock. The probe 10 may include a body 11, an ultrasonic sensor 13, first and second connection cables 15a and 15b, and a guide holder 17.

The body 11 is formed to have a predetermined length into which the suction needle unit 30 can be inserted. An ultrasonic sensor 13 for imaging an embryo of an ovary and an uterus in an ovary of a subject livestock can be disposed in a front lower portion of the body 11 and a guide holder 17 can be removably arranged.

The ultrasonic sensor 13 transmits a detection signal to the ultrasound imaging unit 90 through the first and second connection cables 15a and 15b. The first connection cable 15a is disposed inside the body 11, and one end thereof is electrically connected to the ultrasonic sensor 13. The second connection cable 15b is located outside the body 11 and has one end connected to the other end of the first connection cable 15a and the other end electrically connected to the ultrasound imaging unit 90.

The guide holder 17 is made of a metal material and has a through hole through which the suction needle 31 is inserted and guided. In this case, the through hole has an inner diameter corresponding to the outer diameter of the suction needle 31 to such an extent that the suction needle 31 does not shake when moving back and forth.

The guide holder 17 may be provided to have various sizes corresponding to the outer diameters of the suction needles 30 so that any one of the suction needles 30 having various sizes of outer diameters can be used. Accordingly, when the suction needle 30 is replaced, the guide holder 17 having an inner diameter corresponding to the outer diameter of the suction needle 30 can be used in replacement.

The body 11 may be composed of two symmetrically formed pieces and may be detachably coupled to each other by a plurality of fastening screws 12. [ The body 11 is detached from the body 11 by securing the replacement guide holder 17 to the body 11 after removing the guide holder 17 provided with the plurality of screws 12 when the guide holder 17 is replaced, The body 11 made of two pieces can be coupled through the plurality of fastening screws 12.

In this case, the body 11 is formed with a mounting groove 14 into which the guide holder 17 can be inserted, and the shape of the mounting groove 14 is formed to correspond to the outer shape of the guide holder 17 desirable. The mounting groove 14 is formed with a through-hole 14a through which the suction needle 31 passes. The through-hole 14a allows the suction needle 31 to protrude outward from the front of the body 11.

The body 11 is formed with a guide hole 18 into which the suction needle unit 30 can be inserted from the rear of the body 11 forward. The guide hole 18 guides the suction needle unit 30 so as to be movable back and forth.

Since the probe 10 constructed as described above is easy to insert and separate the suction needle unit 30, the suction needle unit 30 is pulled out from the guide hole 18 during the operation and the suction needle unit 30 is inserted into the suction needle unit 30, After the replacement of the second guide 31, the guide hole 18 is inserted again and the procedure can be continued.

In addition, in the case of carrying out the picking from a plurality of livestock, the animal condom 15 wrapped around the tip of the probe 10 without re-washing, drying and re-sterilization of the picking device 1, Since the operation can be continuously performed by exchanging only the replacement suction needle 31, the operation time and cost can be reduced.

The suction needle unit 30 may include a suction needle 31, a suction needle holder 33, a pipe 35, and a medical tube 37.

2 and 3, the suction needle 31 is disposable and has a length of about 76 mm and an outer diameter of 1.5 mm. The suction needle 31 is coupled to a hub 32 that can be connected to the suction needle holder 33 at a rear end thereof. 2, the suction needle 31 is formed to have a first angle?, For example, about 15 to 16 degrees, so that the tip 31a is suitable for piercing the livestock skin, and the second angle? For example, from about 56 degrees to about 57 degrees. Accordingly, the suction needle 31 minimizes the resistance to repetitive operations of sucking the egg while piercing it several tens of times when the egg is inhaled from the ovary, and greatly improves durability.

The suction needle 31 forms a blast treatment section 31b through blast treatment at its tip end. Accordingly, it is possible to prevent the light from being reflected on the front end surface of the suction needle 30 when the ultrasonic imaging is performed, thereby preventing the ultrasound image from being distorted and preventing the operation site from being seen properly.

Referring to Fig. 4, the suction needle 31 is coated with the silicon layer 31c on the outer periphery and the inner periphery of the distal end portion 31a. Such a cone cone layer 31c is formed in such a manner that even when the distal end portion 31a of the suction needle 31 is repeatedly stuck to the ovaries f1 and f2 (see FIG. 6) and the follicle (see FIG. 9) 31a can be prevented from being dulled. In addition, when the egg (X) or the embryo (Y) flows into the suction needle (31) of the metal material, it passes safely through the suction needle (31) by the smooth silicon layer (31c) (Y) survival rate can be reduced.

The suction needle holder 33 penetrates the inside of the suction needle holder 33 and serves to interconnect the suction needle 31 and the medical tube 37.

The tip 33a of the suction needle holder 33 is detachably inserted into the hub 32 of the suction needle 31. [ After use, the disposable suction needle 31 is separated into a suction needle holder 33 and discarded. As the suction needle 31 is guided by the guide holder 17 in a state where the suction needle unit 30 is inserted into the probe 10, the operation can be performed safely and easily.

The rear end portion 33b of the suction needle holder 33 is inserted and fixed to one end of the pipe 35 and the extension portion 33c extended to the rear end portion 33b is inserted and fixed to the distal end of the medical tube 37. [ Accordingly, the pipe 35 and the medical tube 37 are simultaneously fixed to the suction needle holder 33.

The pipe 35 is in the shape of a barrel made of a metal material, and the medical tube 37 is inserted into the inside and the length thereof can be made about 600 mm. The pipe 35 can be inserted into the guide hole 31 of the body 11 such that the pipe 35 can be moved back and forth. The pipe 35 may be inserted into the ovary f1 or f2 and the follicle e with the distal end portion 31a of the suction needle 31 in order to suck the ovary X or the embryo Y during the procedure, 31).

The medical tube 37 may be made of a flexible transparent polyvinyl chloride (PVC). The length of the medical tube 37 may be about 1500 mm, but the present invention is not limited thereto.

Since the vessel 50 is formed of a transparent material capable of containing the egg X and the egg Y taken in through the suction needle unit 30, the collecting amount of the inoculated egg X or the egg Y It can be judged.

The container vessel 50 is detachably coupled to the stopper 51 at the opening 50a. The stopper 51 is connected to the first connection pipe 53 connected to the medical tube 37 and the second connection pipe 55 connected to the air tube 71 to be described later,

The first connection pipe 53 guides the egg X or the embryo Y sucked through the suction needle unit 30 to the vessel 50. The second connection pipe 55 communicates the vacuum pump 70 and the packed container 50 so that a negative pressure is formed inside the packed container 50. In this case, when a negative pressure is formed in the vessel 50, a suction force acts on the inside of the suction needle unit 30 to suck the egg X and the fertilization egg Y,

Since the survival rate of the egg (X) or embryo (Y) collected by inhalation from the genital organs of the subject livestock is sensitive to the external temperature, in order to keep the outside of the vessel 50 at a constant temperature It is preferable to place the water in the water tank 57 containing water maintained at a temperature of 36.2 degrees during the procedure.

The vacuum pump 70 is connected to the inside of the vessel 50 through an air tube 81 and is electrically connected to the foot switch 73 through a cable 75. The foot switch 73 can be operated by the operator pressing the foot and can turn on / off the driving of the vacuum pump 70.

The ultrasound imaging unit 90 is electrically connected to the second connection cable 15b of the probe 10. The ultrasound imaging unit 90 includes a fog e in the ovaries f1 and f2 of the target livestock taken by the ultrasonic sensor 13 disposed inside the distal end portion of the probe 10, And a monitor 91 for displaying an image at a position where the embryo Y is located. The ultrasound imaging unit 90 may include an operation panel 93 that allows a practitioner to perform various operations for ultrasound imaging.

The ultrasound imaging unit 90 is a diagnostic apparatus that is necessary for OPU operation and is manufactured to be portable enough to be moved to the operation site. Such an ultrasound imaging unit 90 may be used in various ways such as early determination of the pregnancy diagnosis of livestock and horses, diagnosis of breeding period disease, confirmation of pregnancy diagnosis after embryo transfer, and the like.

Hereinafter, with reference to FIG. 6 to FIG. 10, the process of filling through the animal husbandry apparatus and the animal husbandry apparatus 1 according to an embodiment of the present invention will be sequentially described.

FIG. 6 is a view showing a state in which a probe is inserted into a genital unit of a suction needle unit and a target livestock, FIG. 7 is a view showing a state in which a suction needle unit is inserted into a probe inserted in a target livestock genital unit, Fig. 8 is a view showing a state in which a practitioner inserts one hand into a rectum of a target livestock for the purpose of performing a procedure. Fig. 9 is a view showing a state in which a preparation for a procedure is completed. FIG. 10 is a view showing a state in which an embryo is picked up through a suction needle in a state in which the distal end of the uterus of the target livestock is lifted with the hand inserted into the rectum .

Before starting the procedure, prepare the cleaning and disinfecting agents, sedatives and anesthetics and syringes, cleaning gauze, work gloves, latex gloves, and animal condoms necessary for the picking work as a preliminary preparation. The second connection cable 15b of the probe 10 is connected to the ultrasound imaging unit 90 to check the operation state and the medical tube 37 is attached to the first connection pipe 53 of the container 70 After connecting the air tube 71 to the second connection pipe 55, the vacuum pump 70 is operated by the foot switch 73 to check whether the air is sucked.

6, the practitioner connects the medical tube 37 to the extension 33c of the suction needle holder 33 and connects the pipe 35 to the rear end 33b of the suction needle holder 33 , And the disposable suction needle (31) is connected to the distal end (33a) of the suction needle holder (33).

For the livestock to be collected, the livestock is calibrated in the treatment correction band (not shown), the feces is removed from the rectum by putting the gloves on the one hand, and the tail of the livestock is lifted and the sediment (rumun) is syringe of about 0.75 to 0.80 ml And 20 ~ 30 seconds later, local anesthesia (ritokine) is applied to the subdural extracapsular anesthesia. Next, clean the vulva of the target livestock thoroughly, clean it with a towel, disinfect it with alcohol, apply gel to the vulva, and finish the preparations for preparation of the target livestock to facilitate the procedure.

The probe 10 puts an animal condom 95 on the distal end of the probe 10 for reuse and hygiene and inserts the prepared probe 10 into the subject's livestock vial by spreading the sequence of the vocal cords of the target livestock prepared for the surgical correction. At this time, the probe 10 moves to a position where the ultrasonic sensor 13 can reach the genital uterus (b) of the target livestock.

Referring to FIG. 7, the prepared suction needle unit 30 is inserted through the guide hole 18 of the probe 10. At this time, the tip of the suction needle (30) is inserted from the tip of the suction needle (31), and the tip end of the suction needle (30) is arranged to a position adjacent to the through groove (14a).

8, the practitioner then administers a bovine correction and stabilizer, inserts an ultrasound probe into the tail portion after local anesthesia, inserts a gloved hand into the rectum of the subject livestock, (f1, f2), the ovary f1 is held up by the fingers so that the follicle (e) can easily be pierced, and is made to correspond to the tip of the approximate suction needle (31).

After the inhalation of the follicles, the eggs are temporarily stored in a container, and then transferred to a laboratory for processing and storing the eggs.

9, the operator searches for the follicle (e) or the egg (X) in the ovary f1 while watching the image displayed on the monitor 91 of the ultrasound imaging unit 90 in this state. When the follicle (e) or the egg (X) is found, the follicle (e) surrounding the oocyte (X) is transferred to the suction needle tip When the foot switch 73 of the vacuum pump 70 is operated by foot, the egg X is naturally collected by the negative pressure formed inside the vessel vessel 50 into the vessel vessel 70, .

10, in the case of harvesting the embryo Y, the uterine tip end portion (d) is held by the hand inserted into the rectum of the target livestock, and the position of the embryo Y is approximately positioned at the distal end of the suction needle 31 Match.

The foot switch 73 of the vacuum pump 70 is operated with the foot to form the inside of the picking container 50 when the embryo transfer unit Y is found through the monitor 91 of the ultrasound imaging unit 90 The embryo Y can naturally be collected by the suction needle unit 30 through the suction cup unit 70 by the negative pressure applied thereto.

The operator repeatedly observes the monitor 91 of the ultrasound imaging unit 90 and repeats the operation until the follicle e is on the monitor 91 until there is no egg Y. As a result, ) Or embryo (Y) can be completed.

The high-functioning oocytes obtained by the procedure of the present invention can produce fertilized eggs of high-ability superior lineage by using sperm (frozen semen) having superior blood line and extracorporeal artificial insemination to contribute to embryo weighing and mass propagation of high-ability blood line . In addition, the fertilized eggs of the high-yielding herb line (pregnant cows) collected by the procedure of the present invention are transplanted into a large number of surrogate mothers to produce a large number of calves having a high-abundance blood line, It can contribute to securing the hard work of livestock farmers.

The practitioner can perform the operation while ensuring the health of the target animal and the safety of the reproductive organs while watching the image displayed on the monitor 91 as described above. In addition, it is possible to secure a large amount of eggs (X) or fertilized eggs (Y) by a single operation, and to simplify the picking process and shorten the picking time, it is possible to remarkably improve the efficiency of weighing and reproduction of livestock and animals have.

In the foregoing, preferred embodiments of the present invention have been described with reference to the accompanying drawings. Hereinafter, terms and words used in the specification and claims should not be construed as limited to ordinary or dictionary terms, and should be construed as a concept of beauty that meets the technical idea of the present invention. In addition, since the embodiments described in the present specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention, It should be understood that various equivalents and modifications are possible.

10: probe 11: body
13: ultrasonic sensors 15a, 15b: first and second connection cables
17: guide holder 30: suction needle unit
31: suction needle 33: suction needle holder
35: Pipe 37: Medical tube
50: Charging vessel 70: Vacuum pump
90: ultrasound imaging unit

Claims

A probe having an ultrasonic sensor at its tip, the probe being inserted into a uterus of a target animal;
A suction needle unit inserted into the probe so as to be able to move back and forth along the inside of the probe and to suck in the uterus or the embryo;
A collecting container connected to the suction needle unit for collecting eggs or embryos collected from the suction needle unit;
A vacuum pump for sucking an oocyte or a fertilized egg into the suction needle unit by forming a suction force inside the suction needle unit by forming a negative pressure inside the packed vessel; And
And an ultrasound imaging unit for receiving and displaying an image captured by the ultrasonic sensor.

The method according to claim 1,
Wherein the suction needle unit comprises:
A medical tube connected to the container vessel;
A pipe into which the medical tube is inserted;
A suction needle holder to which the medical tube and the pipe are detachably connected; And
And a suction needle connected to the suction needle holder to communicate with the medical tube through the suction needle holder.

3. The method of claim 2,
Wherein the suction needle is coated with a silicon layer on the outer periphery and inside of the tip portion.

3. The method of claim 2,
Wherein the suction needle is formed to have a first angle (?) Of 15 to 16 degrees and a second angle (?) Of 56 to 57 degrees.

3. The method of claim 2,
Wherein the suction needle has a blast treatment portion formed on the outer periphery of the tip portion.

3. The method of claim 2,
Wherein the pipe is formed in a shape of a barrel of a metal material.

3. The method of claim 2,
Wherein the probe includes a guide holder detachably disposed inside the tip of the probe to guide the forward and backward movement of the suction needle.

The method according to claim 1,
Wherein the vacuum pump is electrically connected to a foot switch capable of turning on and off the driving of the vacuum pump by the operator pressing the foot.