WO2023102772A1 - Apparatus for facilitating detection of physiological indicators of small animals and use method thereof - Google Patents

Abstract

An apparatus for facilitating the detection of physiological indicators of small animals and a use method thereof, the apparatus comprising a support structure (10), and an apparatus main body (20), an elastic tail root fixing assembly (30), and a tail sleeve (40) supported on the support structure (10). The apparatus main body (20) is made of a transparent material, so that the real-time state of a small animal can be conveniently observed and recorded at multiple angles. The apparatus main body (20) is also provided with an opening (221) at the bottom thereof, so that the tail of the small animal can naturally extend out, and excreta of the small animal can be conveniently collected in real time. The elastic tail root fixing assembly (30) secures the small animal in an unrestrained elastic fixing manner, so that the small animal can maintain a quiet and stable state in the apparatus main body, thereby preventing the small animal from experiencing a stress reaction and rolling freely, avoiding the entanglement or disconnection of various device wires connected to the body of the small animal. The tail sleeve (40) can stay within a certain temperature range, allowing the tail of the small animal to be comfortably placed and facilitating experimental sampling operations.

Description

Auxiliary device for detecting physiological indicators of small animals and its application method technical field

The invention relates to the technical field of animal nerve function research, in particular to an auxiliary device for detecting physiological indicators of small animals and a usage method thereof.

Background technique

In animal neurological function research, it is often necessary to detect changes in animal physiological indicators. However, in the process of detecting animal physiological indicators, the grasping operation of researchers often causes the animal's stress response, which leads to inaccurate measurement results, especially small animals such as experimental mice, which are especially prone to stress response. In addition, in neuroscience research, it is often necessary to connect external devices to awake animals for wired stimulation or detection. Animals moving around after connecting wired devices may easily cause external wires to be entangled or even interrupt the connection, and animals may also feel nervous after the wires are entangled. stress response.

At present, in this field, there are also some devices for immobilizing small animals when detecting changes in animal physiological indicators, such as restraint devices and light-regulated animal blood sugar real-time monitoring devices, wherein restraint devices are used to fix small animals for intravenous injection. Injection and blood collection, however, this type of device performs experimental operations and tests by completely restraining small animals, and it is obvious that this type of device will cause stress to small animals. The device for real-time monitoring of blood glucose in light-regulated animals is used for real-time detection of tail vein blood glucose concentration in animals stimulated by optogenetics. Specifically, the light-regulated animal blood glucose real-time monitoring device buckles the animal in an opaque cylinder with a lower opening twice the volume of the animal, and opens several small holes around the top and lower end of the cylinder to facilitate light stimulation and tail detection of blood glucose. In this environment, the small animal faces a closed cover with little light, which cannot meet the needs of real-time observation of the whole state, and the small animal can freely rotate inside. When the small animal is connected with a relatively light and fragile equipment line It is easy to cause the equipment line to be entangled or broken.

Generally speaking, the current devices used to fix small animals to assist in the detection of changes in animal physiological indicators have problems such as easily causing stress reactions and inconvenient observation in animals, and there are problems such as poor animal fixation, and animals can move freely and turn around. However, the problem of entanglement or disconnection of the equipment lines connected to the body cannot ensure the accuracy of the detection of changes in physiological indicators of animals.

Contents of the invention

Based on this, an object of the present invention is to provide an auxiliary device for the detection of small animal physiological indicators and a method of use thereof. The device can open and elastically fix the small animal, so that the small animal's body is loose and semi-free, and the field of vision is wide. After a short period of adaptation, it can stay in place naturally and quietly, which can prevent the small animal from producing a stress response and avoiding the small animal spinning around, which is conducive to accurately detecting changes in the physiological indicators of the small animal.

An auxiliary device for detecting physiological indicators of small animals, comprising a support structure, a device main body supported on the support structure, an elastic tail root fixing component, and a tail sleeve tube;

Wherein the device main body includes a support part movably supported on the support structure and a funnel part extending from the support part, the funnel part is used for accommodating small animals and the bottom is provided with a bottom opening, and the bottom opening It is used for the tail of small animals to fall naturally and to facilitate the collection of excrement of small animals;

Wherein the elastic tail root fixing component is arranged between the bottom opening and the tail sleeve tube, and is used to fix the tail root of the small animal;

Wherein the tail sleeve tube is used to fix the rear part of the tail of the small animal, and the temperature of the tail sleeve tube is set and maintained within a certain temperature range to ensure that the tail of the small animal can be placed comfortably and to facilitate experimental sampling operations.

In one embodiment of the present invention, the main body of the device is made of transparent material, and the inner and outer surfaces are smooth, so as to avoid the small animals from being blocked, and facilitate the observation and recording of the real-time status of the small animals.

In one embodiment of the present invention, the elastic tail root fixing assembly includes an adhesive tape, a clip, an elastic member and a tail root fixing ring, wherein the adhesive tape is used to stick the tail root protruding from the bottom opening, the The clip is used to clamp the clampable part of the adhesive tape, and the elastic member is used to bind and fix the clip to the tail root fixing ring, so as to keep the small animal within an autonomous elastic range, And the head of the small animal can face the fixed side of the funnel without turning around, so as to keep all kinds of connections on the small animal quiet and stable.

In an embodiment of the present invention, the tail root fixing ring has two fixing ears, and after the elastic member binds and fixes the clip, two ends thereof are respectively fixed to the corresponding fixing ears.

In one embodiment of the present invention, the supporting structure includes a supporting base, an L-shaped bracket connected to the supporting base, and a supporting ring connected to the L-shaped bracket, and the supporting part of the device body is a cylinder shaped structure and the top is extended to form an extension flange, the support part is movably sleeved in the support ring, and can form a state where the bottom surface of the extension flange is opposed to the top surface of the support ring, In order to be able to adjust the state of the main body of the device by lifting the supporting part, it is convenient to induce small animals to actively climb and protrude their tails from the bottom opening of the funnel part.

In an embodiment of the present invention, the support part has a top opening, so that the connecting wire of the external detection device can be introduced into the funnel part through the top opening and connected to the small animal.

In an embodiment of the present invention, the support structure further includes a first support column connecting the support base and the tail root fixing ring, and a second support column connecting the support base and the tail sleeve tube.

A method for using an auxiliary device for detecting physiological indicators of small animals, comprising steps:

S1. Place the small animal in the funnel of the auxiliary device, so that the tail of the small animal naturally sticks out of the funnel;

S2. Adhere the base of the tail of the small animal with sticky tape, clamp the grippable part of the sticky tape with a clip, bind the clip with an elastic piece and fix both ends of the elastic piece to the tail root fixing ring superior;

S3, inserting the rear part of the tail of the small animal into the tail sleeve tube, and maintaining the preset temperature of the tail sleeve tube; and

S4. Connect the connection line of the detection device to the connection end of the small animal.

In an embodiment of the present invention, the step S1 includes the steps of:

Putting small animals into the infundibulum for many times to move freely to familiarize themselves with the environment of the infundibulum; and

The main body of the device is lifted obliquely so that the small animal actively climbs to induce it to protrude its tail through the bottom opening of the funnel.

In an embodiment of the present invention, the method for using the auxiliary device for detection of small animal physiological indicators further includes the step: S5, after the small animal adapts to the connection line connected to the detection device, perform optogenetic stimulation, electrical stimulation, etc. Any one or more of the experimental operations of stimulation, catheter administration, catheter collection of body fluids, tail blood collection, and electromyography collection.

The auxiliary device for the detection of small animal physiological indicators of the present invention adopts an open elastic fixation method to fix the small animal, so that the small animal can be naturally and elastically maintained in a quiet and stable state, thereby preventing the small animal from producing stress responses and freedom. Rotating, thereby avoiding entanglement or disconnection of various equipment cables connected to small animals, especially light-weight filament wires will not be bitten off by small animals or torn off due to small animals spinning and winding, which can be done To reduce the foreign body sensation on small animals and smoothly connect various precision testing equipment.

The device main body of the auxiliary device for small animal physiological index detection of the present invention is made of transparent materials, which can facilitate multi-angle naked eye observation or use a camera to record the real-time state of small animals in the device main body, satisfying real-time Observe the full state as needed.

The main body of the auxiliary device for the detection of small animal physiological indicators of the present invention is provided with the bottom opening, which not only facilitates the natural extension of the tail of the small animal, but also facilitates the real-time collection of excrement of the small animal.

The tail sleeve tube used in the auxiliary device for the detection of small animal physiological indicators of the present invention can be kept within a certain temperature range, such as within 25°C to 37°C, that is, kept at a relatively comfortable temperature for small animals Within the range, the tail of the small animal can be comfortably placed, and at the same time, it is convenient for experimental operations, such as blood collection from the tail of the small animal.

Further objects and advantages of the invention will fully appear from an understanding of the ensuing description and accompanying drawings.

Description of drawings

Fig. 1 is a schematic structural diagram of the auxiliary device for detecting physiological indicators of small animals according to a preferred embodiment of the present invention.

Fig. 2 is a flow chart of the method for using the auxiliary device for detecting physiological indicators of small animals according to the above selected embodiment of the present invention.

Explanation of reference numerals: support structure 10; support base 11; L-shaped bracket 12; support ring 13; first support column 14; second support column 15; device main body 20; ; Funnel 22; Bottom opening 221; Elastic tail root fixing assembly 30; Adhesive tape 31; Clip 32;

Detailed ways

The following description serves to disclose the present invention to enable those skilled in the art to carry out the present invention. The preferred embodiments described below are only examples, and those skilled in the art can devise other obvious variations. The basic principles of the present invention defined in the following description can be applied to other embodiments, variations, improvements, equivalents and other technical solutions without departing from the spirit and scope of the present invention.

Those skilled in the art should understand that, in the disclosure of the present invention, the terms "vertical", "transverse", "upper", "lower", "front", "rear", "left", "right", The orientations or positional relationships indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present invention and simplified description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, so the above terms should not be construed as limiting the present invention.

It can be understood that the term "a" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element can be one, while in another embodiment, the number of the element The quantity can be multiple, and the term "a" cannot be understood as a limitation on the quantity.

In the description of the present invention, it should be noted that unless otherwise specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection. Connected, or integrally connected; it can be mechanically connected, or electrically connected, or can communicate with each other; it can be directly connected, or indirectly connected through an intermediary, and it can be the internal communication of two components or the interaction of two components relation. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention according to specific situations.

The present invention provides an open elastic auxiliary device for fixing small animals, so as to achieve loose and semi-free bodies of small animals, wide field of vision, and to stay in place naturally and quietly after a short period of adaptation, thereby avoiding stress reactions of small animals And free rotation, to ensure accurate detection of changes in the physiological indicators of small animals, to provide accurate experimental basis for the study of animal nerve function.

As shown in FIG. 1 , the specific structure of an auxiliary device for detecting physiological indicators of small animals according to the present invention is clarified. The auxiliary device for small animal physiological index detection includes a support structure 10, a device main body 20 supported on the support structure 10, an elastic tail root fixing assembly 30, and a tail sleeve tube 40; wherein the device main body 20 includes a Movably supported on the support part 21 of the support structure 10 and the funnel part 22 extending from the support part 21, the funnel part 22 is used to accommodate small animals and the bottom is provided with a bottom opening 221, the bottom opening 221 It is used for the tail of the small animal to fall naturally and to facilitate the collection of excrement of the small animal; wherein the elastic tail root fixing assembly 30 is arranged between the bottom opening 221 and the tail sleeve tube 40 for fixing the small animal. The root of the tail of the animal; wherein the tail sleeve 40 is used to fix the rear part of the tail of the small animal, and the temperature of the tail sleeve 40 is set and kept within a certain temperature range to ensure that the tail of the small animal can be comfortable Place and facilitate experimental sampling operations.

In particular, the main body 20 of the device is made of transparent material, and the inner and outer surfaces are smooth, so as to avoid the small animal being blocked, and most of the body of the small animal can be seen with the naked eye from multiple angles, thereby facilitating multi-angle naked eye observation. Alternatively, a camera is used to record the real-time state of the small animal in the device main body 20 to meet the needs of real-time observation of the whole state.

Preferably, the device main body 20 is made of acrylic material.

It is worth mentioning that the tail sleeve tube 40 can be connected with an external heating device or coated with a heat preservation bag so that its temperature can be kept within a certain temperature range. The specific temperature maintenance of the tail sleeve tube 40 in the present invention The method is not limited.

In addition, it is also worth mentioning that the temperature of the tail sleeve 40 is preferably kept at 25°C to 37°C, so as to be close to the body temperature of the small animal, and kept within a relatively comfortable temperature range for the small animal. The tail can be comfortably placed and convenient for experimental sampling operations, such as blood collection operations on small animal tails.

Further, the elastic tail root fixing assembly 30 includes an adhesive tape 31, a clip 32, an elastic member 33 and a tail root fixing ring 34, wherein the adhesive tape 31 is used to stick the tail root protruding from the bottom opening 221, so The clip 32 is used to clamp the clampable part of the adhesive tape 31, and the elastic member 33 is used to bind and fix the clip 32 on the tail root fixing ring 34, so as to keep the small animal in a Within the scope of autonomy, and the head of the small animal can face the fixed side of the funnel 22 without turning around, so as to keep all kinds of connections on the small animal quiet and stable.

It is worth mentioning that the adhesive tape 31 is preferably a medical adhesive tape, and the clampable part of the adhesive tape 31 is the part of the adhesive tape 31 other than the tail base of the small animal. The elastic member 33 Preferably a rubber band.

Furthermore, the tail root fixing ring 34 has two fixing ears 341 , and the elastic member 33 binds and fixes the clip 32 and fixes both ends to the corresponding fixing ears 341 respectively.

It can be understood that, by elastically fixing the tail of the small animal through the elastic tail root fixing assembly 30, the small animal can be in a loose and semi-free state, and since the main body 20 of the device is a transparent device, the small animal has a wide field of vision, After a short period of semi-free activities, the small animals can adapt and stay in place naturally and quietly, naturally and flexibly maintain a quiet and stable state, avoiding stress reactions. Simultaneously, because the tail root of the small animal is fixed on the tail root fixing ring 34 via the clip 32 and the elastic member 33, it can keep the small animal with its head facing the fixed side of the funnel within an autonomous elastic range without spinning , so as to keep the connection of various detection equipment quiet and stable.

That is to say, the auxiliary device for the detection of small animal physiological indicators adopts an open elastic fixation method to fix the small animal, so that the small animal can be naturally and elastically maintained in a quiet and stable state, thereby avoiding the stress response and Rotate freely, thereby avoiding entanglement or disconnection of various equipment cables connected to small animals, especially light-weight thin wires, which will not be bitten off by small animals or torn off due to small animals spinning and entangled. To reduce the foreign body sensation on small animals and smoothly connect various precision testing equipment.

It is worth mentioning that the support structure 10 includes a support base 11, an L-shaped bracket 12 connected to the support base 11, and a support ring 13 connected to the L-shaped bracket 12. The support part 21 is a cylindrical structure with an extension flange 211 extending from the top, the support part 21 is movably sleeved in the support ring 13, and can form the bottom surface of the extension flange 211 and the support In a state where the top surfaces of the ring 13 are supported, the device main body 20 can be movably supported on the support structure 10 .

It can be understood that since the main body 20 of the device is movably supported on the L-shaped bracket 12, when the tail of the small animal is inside the funnel, the device can be adjusted by lifting the supporting part 21 The state of the main body 20 , for example, can lift the main body 20 of the device obliquely, so as to induce small animals to actively climb and protrude their tails from the bottom opening 221 of the funnel 22 .

In other words, by setting the device body 20 to be movably supported on the support structure 10, the posture of the small animal inside the device body 20 can be adjusted by adjusting the posture of the device body 20, so as to To meet the needs of different experiments, while avoiding the stress response of small animals caused by adjustment methods such as direct grasping.

It is worth mentioning that the support part 21 has a top opening 212, so that the connecting wire of the external detection equipment can be introduced into the funnel part 22 through the top opening 212, and connected to the small animal.

Further, the support structure 10 also includes a first support column 14 connecting the support base 11 and the tail root fixing ring 34 and a second support column 15 connecting the support base 11 and the tail sleeve tube 40 .

It is worth mentioning that the tail root fixing ring 34 , the tail sleeve tube 40 and the support structure 10 are all preferably made of stainless steel.

In addition, it is also worth mentioning that the small animal used by the auxiliary device of the present invention to assist detection is a mouse, or other small animals, and the present invention does not limit the specific type of small experimental animals.

In this embodiment of the present invention, the specific dimensions of the auxiliary device for the detection of small animal physiological indicators are: the support base 11 is a cuboid structure with a length of 50 cm, a width of 30 cm, and a height of 8 cm. The diameter of the top opening 212 of 20 is 13 cm, the depth of the support part 21 is 6.5 cm, and the depth of the funnel part 22 is 12.5 cm. After the funnel part 22 is expanded, it is fan-shaped, and the radius of the fan is 15 cm. The diameter of the bottom opening 221 is 1 cm, and the distance between the bottom opening 221 and the support base 11 is 15 cm.

It should be understood that, according to different experimental requirements, the auxiliary device for detecting physiological indicators of small animals may have other sizes, which are not limited in the present invention.

In this embodiment of the present invention, the specific steps of using the auxiliary device for the detection of small animal physiological indicators are as follows:

First, the small animal needs to move freely in the funnel 22 of the device main body 20 to get familiar with the environment of the funnel 22, for three consecutive days, about one hour per day.

It is worth mentioning that after the small animals are familiar with the main body 20 of the device, they will climb quietly and stay at the bottom of the funnel 22, some will actively extend their tails out of the bottom opening 221 of the funnel, and some will wrap their tails around the funnel. Inside, the main body 20 of the device can be raised separately and slightly tilted so that the small animal actively climbs and induces its tail to protrude from the opening 221 at the bottom of the funnel.

Secondly, stick the base of the tail with a small piece of medical adhesive tape, and the adhesive tape has a handle so that the small clip is clamped.

Once again, after the tail passes through the tail root fixing ring 34, the tail is inserted into the tail sleeve tube 40, and the heat preservation pouch is assisted on the tail sleeve tube 40 to maintain a certain temperature, preferably close to the body temperature of the small animal.

Finally, connect the equipment that needs to be connected to the small animal to the connection end of the small animal. After the small animal adapts for 40 minutes, specific experimental operations can be performed, such as: optogenetic stimulation, electrical stimulation, catheter administration, catheter collection of body fluids, tail Blood collection, EMG collection, etc.

That is to say, as shown in FIG. 2, the present invention also provides a method for using the auxiliary device for detecting physiological indicators of small animals in another aspect, including steps:

S1. Place the small animal in the funnel 22 of the auxiliary device, so that the tail of the small animal naturally sticks out of the funnel 22;

S2. Stick the base of the tail of the small animal with adhesive tape 31, clamp the grippable part of the adhesive tape 31 with a clip, bind the clip 32 with an elastic member 33 and connect the two ends of the elastic member 33 Be fixed on the tail root fixing ring 34;

S3. Insert the rear part of the tail of the small animal into the tail sleeve 40, and keep the tail sleeve 40 at a preset temperature; and

S4. Connect the connection line of the detection device to the connection end of the small animal.

Specifically, the step S1 includes the steps of:

Putting small animals into the funnel 22 for many times to move freely to be familiar with the environment of the funnel 22; and

The device main body 20 is tilted and lifted so that the small animal actively climbs and induces its tail to stretch out through the bottom opening 221 of the funnel portion 22.

Further, the method for using the auxiliary device for detection of small animal physiological indicators also includes the step: S5, after the small animal adapts to the connection line connected to the detection device, perform optogenetic stimulation, electrical stimulation, catheter administration, Any one or more of the experimental operations of catheter collection of body fluids, tail blood collection, and electromyography collection.

Generally speaking, the auxiliary device for the detection of small animal physiological indicators has the following advantages:

(1) The small animal is fixed in an open elastic fixing method, so that the small animal can be naturally and elastically kept in a quiet and stable state, thereby preventing the small animal from producing a stress response and spinning around freely;

(2) All kinds of equipment connected to small animals will not be entangled or disconnected, especially light-weight filament wires will not be entangled, torn off or bitten off by small animals, which can reduce foreign objects on small animals Sensitive and smooth connection of various precision equipment;

(3) The main body 20 of the device adopts a transparent material, so as to observe and record the real-time state of the small animal from multiple angles with the naked eye or an ordinary camera;

(4) The tail of the small animal can be elastically stable in the described tail sleeve tube 40 kept within a certain temperature range, and the tail can be comfortably placed and convenient for experimental sampling operation;

(5) The bottom opening 221 of the device main body 20 can facilitate the natural drop and extension of the tail of the small animal, and can also collect the excrement of the small animal in real time.

The technical features of the above embodiments can be combined arbitrarily. To make the description concise, all possible combinations of the technical features in the above embodiments are not described. However, as long as there is no contradiction in the combination of these technical features, they should be It is considered to be within the range described in this specification.

The above examples only express the preferred implementation of the present invention, and the descriptions thereof are relatively specific and detailed, but should not be construed as limiting the scope of the patent for the invention. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.

Claims (10)

1. An auxiliary device for detecting physiological indicators of small animals, characterized in that it includes a support structure, a device main body supported on the support structure, an elastic tail root fixing component, and a tail sleeve tube;

   Wherein the device main body includes a support part movably supported on the support structure and a funnel part extending from the support part, the funnel part is used for accommodating small animals and the bottom is provided with a bottom opening, and the bottom opening It is used for the tail of small animals to fall naturally and to facilitate the collection of excrement of small animals;

   Wherein the elastic tail root fixing component is arranged between the bottom opening and the tail sleeve tube, and is used to fix the tail root of the small animal;

   Wherein the tail sleeve tube is used to fix the rear part of the tail of the small animal, and the temperature of the tail sleeve tube is set and maintained within a certain temperature range to ensure that the tail of the small animal can be placed comfortably and to facilitate experimental sampling operations.
2. The auxiliary device for detecting physiological indicators of small animals according to claim 1, wherein the main body of the device is made of transparent material, and the inner and outer surfaces are smooth.
3. The auxiliary device for small animal physiological index detection according to claim 1 or 2, wherein the elastic tail root fixation assembly includes an adhesive tape, a clip, an elastic member and a tail root fixing ring, wherein the adhesive tape is used for Adhere the root of the tail protruding from the bottom opening, the clip is used to clamp the clampable part of the adhesive tape, and the elastic member is used to bind and fix the clip to the tail root fixing ring .
4. The auxiliary device for detecting physiological indicators of small animals according to claim 3, wherein the tail base fixing ring has two fixing ears, and the elastic member fixes the two ends of the clip respectively after binding and fixing the clip. corresponding to the fixed ears.
5. The auxiliary device for detecting physiological indicators of small animals according to claim 3, wherein the supporting structure comprises a supporting base, an L-shaped bracket connected to the supporting base, and a support connected to the L-shaped bracket ring, the support part of the device main body is a cylindrical structure and the top is extended to form an extension flange, the support part is movably sleeved in the support ring, and can form the bottom surface of the extension flange The state of supporting against the top surface of the support ring.
6. The auxiliary device for the detection of small animal physiological indicators according to claim 5, wherein the support part has a top opening, so that the connection line of the external detection equipment can be introduced into the funnel part from the top opening, And connected to small animals.
7. The auxiliary device for detecting physiological indicators of small animals according to claim 5, wherein the support structure further includes a first support column connecting the support base and the tail root fixing ring and a first support column connecting the support base. and the second support post of the tail cannula.
8. A method for using an auxiliary device for the detection of small animal physiological indicators, characterized in that it comprises the steps of:

   S1. Place the small animal in the funnel of the auxiliary device, so that the tail of the small animal naturally sticks out of the funnel;

   S2. Adhere the base of the tail of the small animal with sticky tape, clamp the grippable part of the sticky tape with a clip, bind the clip with an elastic piece and fix both ends of the elastic piece to the tail root fixing ring superior;

   S3, inserting the rear part of the tail of the small animal into the tail sleeve tube, and maintaining the preset temperature of the tail sleeve tube; and

   S4. Connect the connection line of the detection device to the connection end of the small animal.
9. The method for using an auxiliary device for detecting physiological indicators of small animals according to claim 8, wherein said step S1 comprises the steps of:

   Putting small animals into the infundibulum for many times to move freely to familiarize themselves with the environment of the infundibulum; and

   The main body of the device is lifted obliquely so that the small animal actively climbs to induce it to protrude its tail through the bottom opening of the funnel.
10. The method for using the auxiliary device for the detection of small animal physiological indicators according to claim 8, further comprising steps: S5, after the small animal adapts to the connection line connected to the detection device, performing optogenetic stimulation on the small animal, Any one or more experimental operations of electrical stimulation, catheter drug delivery, catheter collection of body fluids, tail blood collection, and electromyography collection.

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