JP2011177331A - Blood pressure and respiration measuring apparatus for small animal - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To attach a strain sensor of a respiration measuring device to an implement for fixing the body of a waking animal when a non-invasive sphygmomanometer for small animals is used. <P>SOLUTION: The blood pressure and respiration measuring apparatus includes a portion 11 for fixing the head, chest and forelegs of an animal from the head side and side surfaces, and a portion 12 for fixing the belly, loins and hind legs from side surfaces. By attaching the strain sensor 24 of the respiration measuring device to the chest and belly where the body of the animal is partially exposed, the respiration can be simultaneously measured while the blood pressure is measured with the body of the waking animal fixed by the apparatus. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a method for measuring respiration and blood pressure of awake small animals.

Conventionally, a simple method for measuring the blood pressure of a test animal (mainly a rat) at awakening is to use a non-invasive sphygmomanometer with a cuff wrapped around the tail. At that time, it is necessary to immobilize the body and tail of the subject animal in some way in order to measure blood pressure stably and stably with little stress. For this purpose, a device for fixing the body of the test animal as shown in 26 of FIG. 2 is used.
As a simple method for monitoring respiration, for example, a method of wrapping a strain sensor around the body of a subject animal under anesthesia and detecting its movement as shown in 24 of FIG. 2 is known, but this method is exclusively used. Performed on anesthetized animals, awake animals could not be measured by movements other than animal breathing caused by attaching the sensor.

  In FIG. 2, 22 is connected to 21. The animal is trapped in 26, the tail is brought out, the tail is fixed at 25, 22 is worn, and blood pressure is measured. In respiratory measurement, an animal under anesthesia, 24 is wound around its torso, and 24 is connected to 23.

Special table 2008-529585 gazette

  When investigating physiological phenomena in experimental animals as models, it is desirable to be able to observe normal phenomena. However, in many cases, observation of physiological phenomena is carried out in an anesthetized animal, or a procedure is used in which observation is performed after various sensors are attached and recovered by a surgical operation that requires skilled techniques. We could not observe the phenomenon of time easily. However, for example, in blood pressure measurement, there has been known a method for measuring blood pressure during normal times using an instrument for fixing an animal body. This can measure blood pressure relatively easily, but this conventional method has a problem that it cannot be used together with measuring the respiration by movement of the chest and abdomen because of the fixing device.

  The present invention solves the above-mentioned conventional problems, and an object thereof is to easily realize blood pressure and respiration measurement.

  In order to solve the above-mentioned conventional problems, a blood pressure respiration measuring device for small animals of the present invention is a device for simultaneously measuring the blood pressure and respiration of a small animal, and includes a head, a chest, a part for fixing a forelimb, and a lower abdomen. Awakening by attaching a sensor / cuff to the chest and abdomen that exposes the sensor that measures the respiratory rate of the rat, and a part that fixes the lumbar and hind limbs, and a blood pressure sensor / cuff to the tail It is a device that measures the respiration and blood pressure of rats.

  According to the blood pressure respiration measuring apparatus for small animals of the present invention, anyone can easily measure normal blood pressure and respiration of rats.

Schematic of blood pressure respiration measuring device for small animals Schematic diagram of a conventional instrument for fixing a small animal body, blood pressure measuring device, and respiratory measuring device The figure which shows the procedure when fixing the small animal of the fixing device in embodiment of this invention. The figure which equipped the non-invasive blood pressure measuring device and distortion | strain detection type | mold respiration measuring device (d) or the displacement meter respiration measuring device (e) in embodiment of this invention The figure which equip | installed the non-invasive blood pressure measuring device and displacement meter respiration measuring device in embodiment of this invention, and equipped with the brain signal detection electrode which responds to an odor stimulus

  Embodiments of the present invention will be described below with reference to the drawings.

(Embodiment)
FIG. 1 is a schematic view of a fixture according to an embodiment of the present invention. In FIG. 1, 11 is a part for fixing the head, chest, and forelimbs of the animal from the head side and side, and 12 is a part for fixing the lower abdomen, waist, and hind limbs of the animal from the side. 14 is a pedestal, and 15 is a leg fixed to the pedestal. 11 and 12 are movably fixed by screws 15 installed on 14. The side surface 13 is used so as to be sandwiched by 15, and 11 and 12 can move in the horizontal direction of the head-to-tail axis. 16 can fit into 12 and the animal's tail passes through the hole drilled in 16. 16 can be detached from the top of 12 at any time. Reference numerals 12 and 16 are components that function to fix the lower abdomen, lower back and hind limbs of the animal from the side and the caudal side.

  In FIG. 3, a method of fixing an animal is illustrated. Here, 14 and 16 shown in FIG. 1 are omitted. First, invite the animal into 11 and confirm that the head has reached the tip of 11 and then quickly pass the tail through the 16 holes in 12 (A) and move 12 from the tail toward the head. (B) Adjust the distance between 11 and 12 so that the body can be held stably without causing stress to the animal, and finally 12 is stopped at 15 (c).

  FIG. 4 shows a state where a blood pressure measurement device and a respiration measurement device are attached to a fixed animal. Here, 13, 14, 15, and 16 shown in FIG. 1 are omitted. The blood pressure measuring device is preferably a non-invasive type device with a cuff and a sensor attached to the tail. In (d), the respiration measurement device is a contact-type device, in which 24 is wound around the animal's chest and abdomen. Of course, the movement of the chest and abdomen exposed from 11 and 12 is not hindered. In (e), the respiration measurement device is a non-contact type device, and measures the movement of the chest and abdomen accompanying the breathing of the animal with a displacement meter using light or sound. In order to measure the most sensitive part 14, a hole is made in 14 so that the functioning of 41 is not hindered.

According to such a component, the animal body is sandwiched and fixed from both the head side and the tail side, and further, as shown in FIG. 4, the tail is fixed to 25, and a part of the chest, abdomen and tail are exposed. By doing so, a respiratory sensor can be attached to a part of the chest and abdomen, and a blood pressure sensor can be attached to a part of the tail, and anyone can measure respiration and blood pressure simultaneously in an awake animal.
11 may be fixed to 14.
The fixing of 11 and 12 to 15 may be fixed by a pin or a vise in addition to screwing.

  Although the tail was passed through 16 holes in 12 and 12 was moved toward the head from the tail side, 12 and 11 in the state where 16 was first removed were placed adjacent to each other, and the animal was placed on the head. After making it approach in order of 12 and 11 from a part, it is good to adjust the position of 12 and attach 16 to 12 after that, and to fix a body as mentioned above.

  Although the tail was passed through 16 holes in 12 and 12 was moved toward the head from the tail side, the animal's tail and lower body were first fitted into 12 with 16 attached, and then the head and The upper body may be advanced to 11 and the distance between 11 and 12 may be adjusted to fix the body as described above.

  The animals may be rodent small animals such as rats, mice, guinea pigs, hamsters, and gerbils.

  It should be noted that although a part of the hole is formed in 14, a distance may be created between 14, 11, and 12, and 41 may be installed there.

  In addition, the screwing may be one or two or more screws per one place.

  In FIG. 5, 51, 52, 53 instead of 11 shows a state in which the head, chest, and forelimbs are fixed. Here, 13, 14, 15, and 16 shown in FIG. 1 are omitted. 51 and 52 are fixed to 14, and 53 is reversibly fixed to 52. The head is fixed to 14 through 53 and 52, and the chest and forelimbs are fixed to 14 through 51. As a result, the same effect as that of 11 is fixed. 55 is attached to cover the animal's nose and can present any gas. Thereby, in the awakened animal, the olfactory stimulus is presented through 55 while the head is fixed, and the response of the brain to it is measured through 54. The arrow extending from 54 indicates the signal flow of the brain activity to be measured, and the point leads to the recording device. At the same time, blood pressure is measured via 21 and 22 and respiration is measured via 41 and 42.

  It should be noted that the fixing of 53 to 52 may be pin fixing or vise fixing in addition to screwing.

 According to the apparatus for measuring respiratory blood pressure of small animals of the present invention, anyone can easily grasp the physiological state of an animal by simultaneously measuring the blood pressure and respiration of an awakened animal. Physiological response when adding or manipulation. In addition to blood pressure and breathing, other physiological responses such as brain activity can also be measured.

DESCRIPTION OF SYMBOLS 11 Head, chest, forelimb fixing device 12 Lumbar, lower abdominal region, hindlimb fixing device 13 Guide rail 14 Base 15 Leg 16 Lumbar plate 21 Blood pressure measuring device main body 22 Cuff sensor portion 23 Respiratory measuring device main body 24 Sensor portion 25 Tail fixing anchor 26 Body Fixing Device 41 Displacement Sensor Unit 41 Displacement Meter Body 51 Chest / Forelimb Fixing Device 52 Leg 53 Head Fixing Aid 54 Brain Activity Recording Probe 55 Stimulation Mask

Claims (4)

A device for measuring the respiration and blood pressure of small animals,
The head, the chest, the part that fixes the forelimbs,
The lower abdomen, lower back, have a part to fix the hind limbs,
The device can rest the body of an awake animal at rest and measure the respiration and blood pressure of the small animal, exposing a part of the chest, abdomen and tail,
Measure respiration from the movement of the chest and abdomen of the small animal,
An apparatus for measuring blood pressure and respiration of an awake small animal, wherein the blood pressure is measured noninvasively from the tail of the small animal. A device for measuring the respiration and blood pressure of small animals,
The chest, the part that fixes the forelimbs,
Having an auxiliary tool to fix the head,
The lower abdomen, lower back, have a part to fix the hind limbs,
The device can rest the body of an awake animal at rest, exposing part of the head, chest / abdomen, and tail,
Presenting sensory stimuli to the small animals,
Measure brain activity from the head of the small animal,
Measure respiration from the movement of the chest and abdomen of the small animal,
An apparatus for measuring blood pressure and respiration of an awake small animal, wherein the blood pressure is measured noninvasively from the tail of the small animal. The apparatus according to claim 2, wherein the sensory stimulus is visual, auditory, tactile sense including sinus hair, olfactory sense, taste sense, temperature, and pain stimulus. The measurement of respiration is a contact type using a strain sensor, a laser beam, a non-contact type measurement device using sound,
The apparatus of claim 2.

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