JP2019536448A5 - - Google Patents
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- Publication number
- JP2019536448A5 JP2019536448A5 JP2019522971A JP2019522971A JP2019536448A5 JP 2019536448 A5 JP2019536448 A5 JP 2019536448A5 JP 2019522971 A JP2019522971 A JP 2019522971A JP 2019522971 A JP2019522971 A JP 2019522971A JP 2019536448 A5 JP2019536448 A5 JP 2019536448A5
- Authority
- JP
- Japan
- Prior art keywords
- item
- scavenger
- flow cell
- labeled
- oxidative
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 52
- 239000003153 chemical reaction reagent Substances 0.000 claims description 25
- 235000011178 triphosphate Nutrition 0.000 claims description 22
- 239000001226 triphosphate Substances 0.000 claims description 22
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical class OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims description 21
- 230000001590 oxidative effect Effects 0.000 claims description 20
- 239000002516 radical scavenger Substances 0.000 claims description 19
- 238000003776 cleavage reaction Methods 0.000 claims description 17
- 230000007017 scission Effects 0.000 claims description 17
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical group OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 16
- 238000009396 hybridization Methods 0.000 claims description 12
- 239000000872 buffer Substances 0.000 claims description 10
- 125000006239 protecting group Chemical group 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- GKJQHSSYBJHANH-UHFFFAOYSA-N 1,1-bis(sulfanyl)propane-1-sulfonic acid Chemical compound CCC(S)(S)S(O)(=O)=O GKJQHSSYBJHANH-UHFFFAOYSA-N 0.000 claims description 8
- 239000011541 reaction mixture Substances 0.000 claims description 8
- 239000007983 Tris buffer Substances 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 5
- 239000002773 nucleotide Substances 0.000 claims description 5
- 125000003729 nucleotide group Chemical group 0.000 claims description 5
- 238000001712 DNA sequencing Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- LSXWFXONGKSEMY-UHFFFAOYSA-N di-tert-butyl peroxide Chemical group CC(C)(C)OOC(C)(C)C LSXWFXONGKSEMY-UHFFFAOYSA-N 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 150000007523 nucleic acids Chemical class 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
- 239000008000 CHES buffer Substances 0.000 claims description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 3
- XJLXLCWHYSPAJZ-UHFFFAOYSA-N dithiirane Chemical compound C1SS1 XJLXLCWHYSPAJZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- VEVZDKMDCHTMIA-UHFFFAOYSA-N oxadithietane Chemical compound O1CSS1 VEVZDKMDCHTMIA-UHFFFAOYSA-N 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims 2
- -1 triphosphate compound Chemical class 0.000 claims 2
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 1
- 230000003197 catalytic effect Effects 0.000 claims 1
- HMPSOEYFMTWOFC-UHFFFAOYSA-N propane-2,2-dithiol Chemical compound CC(C)(S)S HMPSOEYFMTWOFC-UHFFFAOYSA-N 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 description 7
- 150000003573 thiols Chemical class 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 2
- MKWKNSIESPFAQN-UHFFFAOYSA-N N-cyclohexyl-2-aminoethanesulfonic acid Chemical group OS(=O)(=O)CCNC1CCCCC1 MKWKNSIESPFAQN-UHFFFAOYSA-N 0.000 description 2
- 125000005647 linker group Chemical group 0.000 description 2
- 150000004662 dithiols Chemical group 0.000 description 1
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15/343,279 | 2016-11-04 | ||
| US15/343,279 US10273539B2 (en) | 2015-11-06 | 2016-11-04 | Methods of using nucleotide analogues |
| US15/457,344 | 2017-03-13 | ||
| US15/457,344 US11421264B2 (en) | 2015-11-06 | 2017-03-13 | Thiol-containing cleave reagents and oxidative wash |
| PCT/US2017/058908 WO2018085156A1 (en) | 2016-11-04 | 2017-10-28 | Thiol-containing cleave reagents and oxidative wash |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019536448A JP2019536448A (ja) | 2019-12-19 |
| JP2019536448A5 true JP2019536448A5 (enExample) | 2020-11-12 |
| JP7091325B2 JP7091325B2 (ja) | 2022-06-27 |
Family
ID=62076119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019522971A Active JP7091325B2 (ja) | 2016-11-04 | 2017-10-28 | チオール含有切断試薬および酸化的洗浄液 |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP3535407B1 (enExample) |
| JP (1) | JP7091325B2 (enExample) |
| CN (1) | CN110506121A (enExample) |
| AU (1) | AU2017354876A1 (enExample) |
| CA (1) | CA3041055A1 (enExample) |
| WO (1) | WO2018085156A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2022516684A (ja) * | 2019-01-08 | 2022-03-01 | シンギュラー・ゲノミクス・システムズ・インコーポレイテッド | ヌクレオチドの開裂可能なリンカーおよびその使用 |
| GB2580963C (en) | 2019-02-01 | 2025-09-03 | Hemispherian As | Cancer therapies |
| CN114031476B (zh) * | 2021-11-23 | 2023-12-08 | 北京中医药大学 | 一种将甲硫基亚甲基氧衍生物转化为羟基化合物的绿色方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO873956L (no) * | 1986-09-25 | 1988-03-28 | Bp Chem Int Ltd | Rensemidler inneholdende peroksyder for fremstilling av flyktige sulfider. |
| US6046039A (en) * | 1998-08-19 | 2000-04-04 | Battelle Memorial Institute | Methods for producing partially digested restriction DNA fragments and for producing a partially modified PCR product |
| US6440705B1 (en) * | 1998-10-01 | 2002-08-27 | Vincent P. Stanton, Jr. | Method for analyzing polynucleotides |
| US6566059B1 (en) * | 1998-10-01 | 2003-05-20 | Variagenics, Inc. | Method for analyzing polynucleotides |
| US6465628B1 (en) * | 1999-02-04 | 2002-10-15 | Isis Pharmaceuticals, Inc. | Process for the synthesis of oligomeric compounds |
| US6309836B1 (en) * | 1999-10-05 | 2001-10-30 | Marek Kwiatkowski | Compounds for protecting hydroxyls and methods for their use |
| US6498189B1 (en) * | 1999-11-10 | 2002-12-24 | University Of New Mexico | Method for induction of L-selectin shedding |
| US7057026B2 (en) * | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| JP4230364B2 (ja) * | 2002-04-04 | 2009-02-25 | バイオタージ アクチボラゲット | 新しい方法 |
| US8481259B2 (en) * | 2007-02-05 | 2013-07-09 | Intelligent Bio-Systems, Inc. | Methods and devices for sequencing nucleic acids in smaller batches |
| US8101353B2 (en) * | 2007-12-18 | 2012-01-24 | Advanced Analytical Technologies, Inc. | System and method for nucleotide sequence profiling for sample identification |
| US20100330569A1 (en) * | 2009-04-23 | 2010-12-30 | Intelligent Bio-Systems, Inc. | Hydroxymethyl Linkers For Labeling Nucleotides |
| KR101107315B1 (ko) * | 2009-09-29 | 2012-01-20 | 한국과학기술연구원 | ３′―하이드록실기에 형광을 띄는 장애그룹이 부착된 뉴클레오시드 삼인산을 가역적 종결자로서 이용한 ｄｎａ 염기서열 분석 방법 |
| WO2012149472A2 (en) * | 2011-04-27 | 2012-11-01 | Ignite Institute For Individualized Health | Methods, compositions, and kits for treating and preventing neurological conditions |
| WO2015006811A2 (en) * | 2013-07-19 | 2015-01-22 | The Walter And Eliza Hall Institute Of Medical Research | Diagnostic methods |
| EP2876166B1 (en) * | 2013-11-20 | 2016-12-14 | Roche Diagnostics GmbH | New compound for sequencing by synthesis |
-
2017
- 2017-10-28 CA CA3041055A patent/CA3041055A1/en active Pending
- 2017-10-28 EP EP17868018.7A patent/EP3535407B1/en active Active
- 2017-10-28 JP JP2019522971A patent/JP7091325B2/ja active Active
- 2017-10-28 AU AU2017354876A patent/AU2017354876A1/en not_active Abandoned
- 2017-10-28 CN CN201780080043.7A patent/CN110506121A/zh active Pending
- 2017-10-28 WO PCT/US2017/058908 patent/WO2018085156A1/en not_active Ceased
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