JP2019535321A - タンパク質ライゲーションの方法及びその使用 - Google Patents

タンパク質ライゲーションの方法及びその使用 Download PDF

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Publication number
JP2019535321A
JP2019535321A JP2019547066A JP2019547066A JP2019535321A JP 2019535321 A JP2019535321 A JP 2019535321A JP 2019547066 A JP2019547066 A JP 2019547066A JP 2019547066 A JP2019547066 A JP 2019547066A JP 2019535321 A JP2019535321 A JP 2019535321A
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Japan
Prior art keywords
amino acid
peptide
protein
reactive
glutamate
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JP2019547066A
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Japanese (ja)
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JP2019535321A5 (enExample
Inventor
ジョン スクワイア クリストファー
ジョン スクワイア クリストファー
ニール ベイカー エドワード
ニール ベイカー エドワード
ゲイリー ヤング ポール
ゲイリー ヤング ポール
ヨサアットマディヤ ユリアナ
ヨサアットマディヤ ユリアナ
Original Assignee
オークランド ユニサービシーズ リミティド
オークランド ユニサービシーズ リミティド
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Publication of JP2019535321A publication Critical patent/JP2019535321A/ja
Publication of JP2019535321A5 publication Critical patent/JP2019535321A5/ja
Priority to JP2022181857A priority Critical patent/JP2023016842A/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2019547066A 2016-11-16 2017-11-16 タンパク質ライゲーションの方法及びその使用 Pending JP2019535321A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022181857A JP2023016842A (ja) 2016-11-16 2022-11-14 タンパク質ライゲーションの方法及びその使用

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NZ726413 2016-11-16
NZ72641316 2016-11-16
PCT/NZ2017/050147 WO2018093274A1 (en) 2016-11-16 2017-11-16 Methods for protein ligation and uses thereof

Related Child Applications (1)

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JP2022181857A Division JP2023016842A (ja) 2016-11-16 2022-11-14 タンパク質ライゲーションの方法及びその使用

Publications (2)

Publication Number Publication Date
JP2019535321A true JP2019535321A (ja) 2019-12-12
JP2019535321A5 JP2019535321A5 (enExample) 2020-12-24

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JP2019547066A Pending JP2019535321A (ja) 2016-11-16 2017-11-16 タンパク質ライゲーションの方法及びその使用
JP2022181857A Pending JP2023016842A (ja) 2016-11-16 2022-11-14 タンパク質ライゲーションの方法及びその使用

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Country Status (5)

Country Link
US (1) US11807671B2 (enExample)
EP (1) EP3541848A4 (enExample)
JP (2) JP2019535321A (enExample)
CN (1) CN110177811B (enExample)
WO (1) WO2018093274A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024071043A1 (ja) * 2022-09-30 2024-04-04 国立大学法人山形大学 ヘテロ多量体タンパク質の製造方法、タンパク質、核酸、発現ベクター、形質転換体、およびタンパク質の製造方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3837553A1 (en) 2018-08-17 2021-06-23 Regeneron Pharmaceuticals, Inc. Methods for de novo protein sequencing
GB201903479D0 (en) * 2019-03-14 2019-05-01 Univ Oxford Innovation Ltd Polypeptide with enchanced rate of spontaneous isopeptide bond formation with it's peptide tag partner and uses thereof
US20220371986A1 (en) * 2019-04-04 2022-11-24 The Regents Of The University Of California Method to generate biochemically reactive amino acids
CN111462813B (zh) * 2020-03-18 2020-10-20 华东师范大学 一种基于α螺旋融合两种蛋白质且保持各自亚基活性的蛋白质融合设计方法
CN112063642B (zh) * 2020-09-04 2022-11-29 湖北大学 一种依赖t5核酸外切酶构建重组质粒的预混液及其应用
WO2024099368A1 (en) * 2022-11-08 2024-05-16 Westlake University Peptide tags, compositions and methods for site-specific protein conjugation
WO2025081445A1 (zh) * 2023-10-20 2025-04-24 南京工业大学 一种高酯键形成效率的Catcher/Tag肽连接体

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2011098772A1 (en) * 2010-02-11 2011-08-18 Isis Innovation Limited Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds

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DK2571510T3 (en) * 2010-05-21 2018-11-19 Xl Protein Gmbh BIOSYNTHETIC PROLIN / ALANIN-RANDOM COIL POLYPEPTIDES AND THEIR APPLICATIONS
FR2973032A1 (fr) 2011-03-24 2012-09-28 Commissariat Energie Atomique Peptides aptes a former un complexe covalent et leurs utilisations
WO2013160453A2 (en) * 2012-04-26 2013-10-31 Iba Gmbh Adapter molecule capable of reversibly equipping a fusion protein carrying an oligohistidine affinity tag with a further affinity tag and methods of using the same

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2011098772A1 (en) * 2010-02-11 2011-08-18 Isis Innovation Limited Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
EDWARD N. BAKER ET AL.: "Self-generated covalent cross-links in the cell-surface adhesins of Gram-positive bacteria", BIOCHEM. SOC. TRANS., vol. 43, JPN6021038949, 2015, pages 787 - 794, ISSN: 0004608584 *
GIANLUCA VEGGIANIA ET AL.: "Programmable polyproteams built using twin peptide superglues", PNAS, vol. 113, no. 5, JPN6021038948, 2 February 2016 (2016-02-02), pages 1202 - 1207, ISSN: 0004608585 *
HANNA KWON ET AL.: "Autocatalytically generated Thr-Gln ester bond cross-links stabilize the repetitive Ig-domain shaft", PNAS, vol. 111, no. 4, JPN6021038952, 28 January 2014 (2014-01-28), pages 1367 - 1372, ISSN: 0004975226 *
MARLENE PROSCHEL ET AL.: "Engineering of metabolic pathways by artificial enzyme channels", FRONTIERS IN BIOENGNEERING AND BIOTECHNOLOGY, vol. 3, JPN6021038950, October 2015 (2015-10-01), pages 168 - 1, ISSN: 0004975227 *
P. G. YOUNG ET AL.: "Harnessing ester bond chemistry for protein ligation", CHEM. COMMUN., vol. 53, JPN6021038947, 3 January 2017 (2017-01-03), pages 1502 - 1505, ISSN: 0004608586 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024071043A1 (ja) * 2022-09-30 2024-04-04 国立大学法人山形大学 ヘテロ多量体タンパク質の製造方法、タンパク質、核酸、発現ベクター、形質転換体、およびタンパク質の製造方法

Also Published As

Publication number Publication date
EP3541848A4 (en) 2020-05-27
CN110177811B (zh) 2024-01-09
EP3541848A1 (en) 2019-09-25
US20190352343A1 (en) 2019-11-21
JP2023016842A (ja) 2023-02-02
CN110177811A (zh) 2019-08-27
WO2018093274A1 (en) 2018-05-24
US11807671B2 (en) 2023-11-07

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