CN110177811B - 用于蛋白质连接的方法及其用途 - Google Patents

用于蛋白质连接的方法及其用途 Download PDF

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Publication number
CN110177811B
CN110177811B CN201780083490.8A CN201780083490A CN110177811B CN 110177811 B CN110177811 B CN 110177811B CN 201780083490 A CN201780083490 A CN 201780083490A CN 110177811 B CN110177811 B CN 110177811B
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China
Prior art keywords
protein
amino acid
binding partner
reactive
peptide
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CN201780083490.8A
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English (en)
Chinese (zh)
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CN110177811A (zh
Inventor
C·J·斯夸尔
E·N·贝克
P·G·扬
Y·约萨特马贾
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Auckland Uniservices Ltd
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Auckland Uniservices Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201780083490.8A 2016-11-16 2017-11-16 用于蛋白质连接的方法及其用途 Expired - Fee Related CN110177811B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NZ726413 2016-11-16
NZ72641316 2016-11-16
PCT/NZ2017/050147 WO2018093274A1 (en) 2016-11-16 2017-11-16 Methods for protein ligation and uses thereof

Publications (2)

Publication Number Publication Date
CN110177811A CN110177811A (zh) 2019-08-27
CN110177811B true CN110177811B (zh) 2024-01-09

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CN201780083490.8A Expired - Fee Related CN110177811B (zh) 2016-11-16 2017-11-16 用于蛋白质连接的方法及其用途

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US (1) US11807671B2 (enExample)
EP (1) EP3541848A4 (enExample)
JP (2) JP2019535321A (enExample)
CN (1) CN110177811B (enExample)
WO (1) WO2018093274A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3837553A1 (en) 2018-08-17 2021-06-23 Regeneron Pharmaceuticals, Inc. Methods for de novo protein sequencing
GB201903479D0 (en) * 2019-03-14 2019-05-01 Univ Oxford Innovation Ltd Polypeptide with enchanced rate of spontaneous isopeptide bond formation with it's peptide tag partner and uses thereof
US20220371986A1 (en) * 2019-04-04 2022-11-24 The Regents Of The University Of California Method to generate biochemically reactive amino acids
CN111462813B (zh) * 2020-03-18 2020-10-20 华东师范大学 一种基于α螺旋融合两种蛋白质且保持各自亚基活性的蛋白质融合设计方法
CN112063642B (zh) * 2020-09-04 2022-11-29 湖北大学 一种依赖t5核酸外切酶构建重组质粒的预混液及其应用
JPWO2024071043A1 (enExample) * 2022-09-30 2024-04-04
WO2024099368A1 (en) * 2022-11-08 2024-05-16 Westlake University Peptide tags, compositions and methods for site-specific protein conjugation
WO2025081445A1 (zh) * 2023-10-20 2025-04-24 南京工业大学 一种高酯键形成效率的Catcher/Tag肽连接体

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011144756A1 (en) * 2010-05-21 2011-11-24 Xl-Protein Gmbh Biosynthetic proline/alanine random coil polypeptides and their uses
WO2012127060A1 (fr) * 2011-03-24 2012-09-27 Commissariat à l'énergie atomique et aux énergies alternatives Peptides aptes à former un complexe covalent et leurs utilisations
EP2534484A1 (en) * 2010-02-11 2012-12-19 Isis Innovation Limited Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds
WO2013160453A2 (en) * 2012-04-26 2013-10-31 Iba Gmbh Adapter molecule capable of reversibly equipping a fusion protein carrying an oligohistidine affinity tag with a further affinity tag and methods of using the same

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
US6766333B1 (en) * 2000-11-08 2004-07-20 Citrix Systems, Inc. Method and apparatus for synchronizing a user interface element displayed on a client and a software application component executing on a web server

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2534484A1 (en) * 2010-02-11 2012-12-19 Isis Innovation Limited Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds
WO2011144756A1 (en) * 2010-05-21 2011-11-24 Xl-Protein Gmbh Biosynthetic proline/alanine random coil polypeptides and their uses
WO2012127060A1 (fr) * 2011-03-24 2012-09-27 Commissariat à l'énergie atomique et aux énergies alternatives Peptides aptes à former un complexe covalent et leurs utilisations
US9481715B2 (en) * 2011-03-24 2016-11-01 Commissariat A L'energie Atomique Et Aux Energies Alternatives Peptides capable of forming a convalent complex, and uses thereof
WO2013160453A2 (en) * 2012-04-26 2013-10-31 Iba Gmbh Adapter molecule capable of reversibly equipping a fusion protein carrying an oligohistidine affinity tag with a further affinity tag and methods of using the same

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
Autocatalytically generated Thr-Gln ester bond cross-links stabilize the repetitive Ig-domain shaft of a bacterial cell surface adhesin;Hanna Kwon等;《PNAS》;20140128;第111卷(第4期);第1367-1372页 *
Engineering of metabolic pathways by artificial enzyme channels;Marlene Pröschel等;《Frontiers in Bioengineering and Biotechnology》;20151021;第3卷(第168期);第1-13页 *
Harnessing ester bond chemistry for protein ligation;P. G. Young等;《ChemComm》;20170103(第9期);第1-4页 *
Programmable polyproteams built using twin peptide superglues;Gianluca Veggiani等;《PNAS》;20160202;第113卷(第5期);第1202–1207页 *
Self-generated covalent cross-links in the cell-surface adhesins of Gram-positive bacteria;Edward N. Baker等;《Biochemical Society Transactions》;20150430;第43卷(第5期);第787–794页 *
Synthetic Protein Scaffolds Based on Peptide Motifs and Cognate Adaptor Domains for Improving Metabolic Productivity;Anselm H. C.Horn等;《Frontiers inBioengineeringandBiotechnology》;20151123;第3卷(第191期);第1-7页 *
蛋白质二硫键异构酶分子间异肽键交联的研究;何红伟;《中国优秀硕士学位论文全文数据库 (基础科学辑)》;20030115(第1期);第1-46页 *
蛋白质交联的研究进展;刘凤华等;《生物技术通报》;20140826(第4期);第1-4页 *

Also Published As

Publication number Publication date
JP2019535321A (ja) 2019-12-12
EP3541848A4 (en) 2020-05-27
EP3541848A1 (en) 2019-09-25
US20190352343A1 (en) 2019-11-21
JP2023016842A (ja) 2023-02-02
CN110177811A (zh) 2019-08-27
WO2018093274A1 (en) 2018-05-24
US11807671B2 (en) 2023-11-07

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