JP2019528762A5 - - Google Patents

Info

Publication number

JP2019528762A5

JP2019528762A5 JP2019517311A JP2019517311A JP2019528762A5 JP 2019528762 A5 JP2019528762 A5 JP 2019528762A5 JP 2019517311 A JP2019517311 A JP 2019517311A JP 2019517311 A JP2019517311 A JP 2019517311A JP 2019528762 A5 JP2019528762 A5 JP 2019528762A5

Authority

JP

Japan

Prior art keywords

sequence

capture

oligonucleotide

cassetteable

hybridization probe

Prior art date

2017-09-29

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 108091034117 Oligonucleotide Proteins 0.000 claims 102
• 238000009396 hybridization Methods 0.000 claims 45
• 239000000523 sample Substances 0.000 claims 45
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 33
• 238000002955 isolation Methods 0.000 claims 29
• 210000004027 cell Anatomy 0.000 claims 27
• 238000000034 method Methods 0.000 claims 26
• 108020004414 DNA Proteins 0.000 claims 17
• 239000007850 fluorescent dye Substances 0.000 claims 15
• 230000000295 complement effect Effects 0.000 claims 13
• 238000004720 dielectrophoresis Methods 0.000 claims 13
• 150000007523 nucleic acids Chemical class 0.000 claims 12
• 108020004707 nucleic acids Proteins 0.000 claims 11
• 102000039446 nucleic acids Human genes 0.000 claims 11
• 230000037452 priming Effects 0.000 claims 11
• 239000002299 complementary DNA Substances 0.000 claims 8
• 239000003153 chemical reaction reagent Substances 0.000 claims 7
• 239000012634 fragment Substances 0.000 claims 6
• 239000002773 nucleotide Substances 0.000 claims 6
• 125000003729 nucleotide group Chemical group 0.000 claims 6
• 238000011065 in-situ storage Methods 0.000 claims 5
• 238000003780 insertion Methods 0.000 claims 5
• 230000037431 insertion Effects 0.000 claims 5
• 108090000623 proteins and genes Proteins 0.000 claims 4
• 108091008875 B cell receptors Proteins 0.000 claims 2
• 108091026890 Coding region Proteins 0.000 claims 2
• 108090000790 Enzymes Proteins 0.000 claims 2
• 102000004190 Enzymes Human genes 0.000 claims 2
• 108091008874 T cell receptors Proteins 0.000 claims 2
• 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims 2
• 238000001514 detection method Methods 0.000 claims 2
• 210000000633 nuclear envelope Anatomy 0.000 claims 2
• 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
• 108020004635 Complementary DNA Proteins 0.000 claims 1
• 210000000170 cell membrane Anatomy 0.000 claims 1
• 108091092330 cytoplasmic RNA Proteins 0.000 claims 1
• 239000012530 fluid Substances 0.000 claims 1
• 230000002934 lysing effect Effects 0.000 claims 1
• 230000002101 lytic effect Effects 0.000 claims 1
• 108020004999 messenger RNA Proteins 0.000 claims 1
• 239000000203 mixture Substances 0.000 claims 1
• 238000012163 sequencing technique Methods 0.000 claims 1
• 238000006467 substitution reaction Methods 0.000 claims 1
• 239000013589 supplement Substances 0.000 claims 1
• 230000001502 supplementing effect Effects 0.000 claims 1
• 238000013518 transcription Methods 0.000 claims 1
• 230000035897 transcription Effects 0.000 claims 1