JP2019519243A5 - - Google Patents

Info

Publication number

JP2019519243A5

JP2019519243A5 JP2018568435A JP2018568435A JP2019519243A5 JP 2019519243 A5 JP2019519243 A5 JP 2019519243A5 JP 2018568435 A JP2018568435 A JP 2018568435A JP 2018568435 A JP2018568435 A JP 2018568435A JP 2019519243 A5 JP2019519243 A5 JP 2019519243A5

Authority

JP

Japan

Prior art keywords

seq

host cell

promoter

genetically engineered

item

Prior art date

2017-06-27

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
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• 108091033319 polynucleotide Proteins 0.000 claims description 60
• 239000002157 polynucleotide Substances 0.000 claims description 60
• 102000040430 polynucleotide Human genes 0.000 claims description 60
• 108090000623 proteins and genes Proteins 0.000 claims description 42
• 238000000034 method Methods 0.000 claims description 36
• 230000000593 degrading effect Effects 0.000 claims description 19
• 239000002773 nucleotide Substances 0.000 claims description 17
• 125000003729 nucleotide group Chemical group 0.000 claims description 17
• 239000002028 Biomass Substances 0.000 claims description 14
• 229920006395 saturated elastomer Polymers 0.000 claims description 14
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 12
• 241000186216 Corynebacterium Species 0.000 claims description 6
• 238000004519 manufacturing process Methods 0.000 claims description 4
• 125000003275 alpha amino acid group Chemical group 0.000 claims 2
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 16
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
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• 235000001014 amino acid Nutrition 0.000 description 8
• 150000001413 amino acids Chemical class 0.000 description 8
• ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 8
• 150000007524 organic acids Chemical class 0.000 description 8
• 108091081024 Start codon Proteins 0.000 description 7
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 4
• 241000186226 Corynebacterium glutamicum Species 0.000 description 4
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 4
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 4
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• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 4
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• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 4
• 239000004472 Lysine Substances 0.000 description 4
• LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 4
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 4
• 239000004473 Threonine Substances 0.000 description 4
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 4
• 150000001298 alcohols Chemical class 0.000 description 4
• 229960001230 asparagine Drugs 0.000 description 4
• 235000009582 asparagine Nutrition 0.000 description 4
• 235000003704 aspartic acid Nutrition 0.000 description 4
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 4
• 235000019441 ethanol Nutrition 0.000 description 4
• 229960000310 isoleucine Drugs 0.000 description 4
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 4
• 229930182817 methionine Natural products 0.000 description 4
• 235000005985 organic acids Nutrition 0.000 description 4
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 4
• KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 4
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 4
• 230000035772 mutation Effects 0.000 description 3
• 238000012258 culturing Methods 0.000 description 1
• 238000012986 modification Methods 0.000 description 1
• 230000004048 modification Effects 0.000 description 1