JP2019510502A5 - - Google Patents
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- JP2019510502A5 JP2019510502A5 JP2018552760A JP2018552760A JP2019510502A5 JP 2019510502 A5 JP2019510502 A5 JP 2019510502A5 JP 2018552760 A JP2018552760 A JP 2018552760A JP 2018552760 A JP2018552760 A JP 2018552760A JP 2019510502 A5 JP2019510502 A5 JP 2019510502A5
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- 210000004027 cells Anatomy 0.000 description 26
- 210000000130 stem cell Anatomy 0.000 description 6
- 238000010899 nucleation Methods 0.000 description 5
- 102100011593 PSMA7 Human genes 0.000 description 3
- 101710033516 PSMA7 Proteins 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 2
- 210000003751 DN2 alpha-beta immature T lymphocyte Anatomy 0.000 description 2
- 210000001086 DN3 alpha-beta immature T lymphocyte Anatomy 0.000 description 2
- 210000001671 Embryonic Stem Cells Anatomy 0.000 description 2
- 210000004263 Induced Pluripotent Stem Cells Anatomy 0.000 description 2
- 210000001778 pluripotent stem cell Anatomy 0.000 description 2
- 210000004504 Adult Stem Cells Anatomy 0.000 description 1
- 210000003719 B-Lymphocytes Anatomy 0.000 description 1
- 210000004700 Fetal Blood Anatomy 0.000 description 1
- 108010002586 Interleukin-7 Proteins 0.000 description 1
- 210000000066 Myeloid Cells Anatomy 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000002771 cell marker Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 230000003247 decreasing Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 101700008686 sca1 Proteins 0.000 description 1
- 101700071491 scaA Proteins 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000001519 tissues Anatomy 0.000 description 1
Description
実施例８：スケールアップ可能なＴ前駆細胞の分化誘導
本明細書において「幹細胞」は、さらに特化した細胞に分化することが可能な、自己複製能を有する細胞を指す。幹細胞としては、胚性幹細胞（ESC）や人工多能性幹細胞（iPSC）などの多能性幹細胞（PSC）；および臍帯血幹細胞や成体幹細胞などの、様々な組織で見出され、複数の細胞種に分化可能な幹細胞が挙げられる。
まず、無血清IMDM+BIT培地中において、10μg/mLのリガンドDL4を吸着させたプレートに播種するソーティング後のsca1+ckit+HSPCの細胞密度を調整した。1000個／ウェル（3125個／cm2）未満の細胞密度では、増殖した総細胞数のばらつきが大きいことが観察された（図４ａ）。さらに、3.1×103個／cm2よりも細胞密度を高くすると、増殖した総細胞数は有意に低下した（図４ａ）。これは、HSPCコンパートメントが本質的にばらつきを持っていることに起因すると考えられ、供給源として播種する細胞をさらに精製することによって、このようなばらつきを排除することができると考えられる。しかし、播種密度を1000個／ウェル以上とすると、増殖した総細胞数の相対値のばらつきは最小となった。播種する細胞密度を様々に変えて試験を行ったところ、各DNサブセットの頻度に有意差は見られなかった（図５ａ）。さらに、播種密度を1000個／ウェル（3125個／cm2）とすると、別の細胞種である骨髄系細胞およびＢ細胞への分化に傾倒した細胞が最も少なくなった（図５ａ）。また、播種密度を1000個／ウェルよりも多くすると、細胞増殖が低下することが観察された。これらを踏まえ、アッセイ最適化の次の段階では、最初に播種するHSPCの播種密度を1000個／ウェル（3125個／cm2）とした。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022018520A JP2022058939A (ja) | 2016-04-08 | 2022-02-09 | 幹細胞および／または前駆細胞からｔ前駆細胞を作製する方法ならびに該ｔ前駆細胞の使用 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662320005P | 2016-04-08 | 2016-04-08 | |
| US62/320,005 | 2016-04-08 | ||
| PCT/CA2017/050428 WO2017173551A1 (en) | 2016-04-08 | 2017-04-07 | Method for generating progenitor t cells from stem and/or progenitor cells and use of same |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022018520A Division JP2022058939A (ja) | 2016-04-08 | 2022-02-09 | 幹細胞および／または前駆細胞からｔ前駆細胞を作製する方法ならびに該ｔ前駆細胞の使用 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019510502A JP2019510502A (ja) | 2019-04-18 |
| JP2019510502A5 true JP2019510502A5 (ja) | 2020-05-07 |
| JP7049261B2 JP7049261B2 (ja) | 2022-04-06 |
Family
ID=60000120
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018552760A Active JP7049261B2 (ja) | 2016-04-08 | 2017-04-07 | 幹細胞および／または前駆細胞からｔ前駆細胞を作製する方法ならびに該ｔ前駆細胞の使用 |
| JP2022018520A Pending JP2022058939A (ja) | 2016-04-08 | 2022-02-09 | 幹細胞および／または前駆細胞からｔ前駆細胞を作製する方法ならびに該ｔ前駆細胞の使用 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022018520A Pending JP2022058939A (ja) | 2016-04-08 | 2022-02-09 | 幹細胞および／または前駆細胞からｔ前駆細胞を作製する方法ならびに該ｔ前駆細胞の使用 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20190142867A1 (ja) |
| EP (1) | EP3440198A4 (ja) |
| JP (2) | JP7049261B2 (ja) |
| CN (1) | CN109312307A (ja) |
| CA (1) | CA3019845A1 (ja) |
| WO (1) | WO2017173551A1 (ja) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110506110A (zh) * | 2017-02-13 | 2019-11-26 | 巴黎公共救济院 | 产生t细胞祖细胞的方法 |
| CA3077344A1 (en) * | 2017-09-29 | 2019-04-04 | Regents Of The University Of Minnesota | Methods of making, expanding, and using a human progenitor t cell |
| CN112041431A (zh) * | 2018-02-14 | 2020-12-04 | 桑尼布鲁克研究所 | 产生t细胞谱系细胞的方法 |
| KR20210008047A (ko) * | 2018-05-11 | 2021-01-20 | 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 | 활성을 증가시키기 위한 면역 세포의 변형 |
| CN112930393A (zh) * | 2018-08-28 | 2021-06-08 | 福瑞德哈金森癌症研究中心 | 结合诱导的Notch信号传导的过继T细胞疗法的方法和组合物 |
| WO2020124256A1 (en) * | 2018-12-21 | 2020-06-25 | Stemcell Technologies Canada Inc. | Media and methods for differentiating natural killer cells |
| CA3142947A1 (en) * | 2019-06-12 | 2020-12-17 | The Regents Of The University Of California | Engineered off-the-shelf immune cells and methods of use thereof |
| EP4058562A4 (en) * | 2019-11-14 | 2023-12-20 | Zandstra, Peter William | MEDIA FORMULATIONS AND METHODS FOR PRODUCING PRECURSOR CELLS |
| US11788065B2 (en) * | 2019-12-23 | 2023-10-17 | Boston Medical Center Corporation | Human iPSC-based derivation of NK and T-cells using early Notch induction |
| EP4352208A1 (en) * | 2021-05-18 | 2024-04-17 | The University of British Columbia | A method for producing blood progenitor and progenitor t cells, resulting cells and methods and uses thereof |
| EP4373920A1 (en) * | 2021-07-19 | 2024-05-29 | Repairon Immuno GmbH | Method of producing a population of immune cells from pluripotent stem cells |
| CN114324126B (zh) * | 2022-01-07 | 2024-03-19 | 南京鼓楼医院 | 一种改变早期b细胞分化的方法 |
| CN117567651B (zh) * | 2024-01-15 | 2024-03-26 | 中国人民解放军东部战区总医院 | 协同表达血管内皮黏附分子vcam1的嵌合抗原受体及其应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2352816B1 (en) * | 2008-11-07 | 2015-06-24 | Sunnybrook Health Sciences Centre | Human progenitor t-cells |
-
2017
- 2017-04-07 CA CA3019845A patent/CA3019845A1/en active Pending
- 2017-04-07 WO PCT/CA2017/050428 patent/WO2017173551A1/en active Application Filing
- 2017-04-07 EP EP17778524.3A patent/EP3440198A4/en active Pending
- 2017-04-07 US US16/091,266 patent/US20190142867A1/en active Pending
- 2017-04-07 CN CN201780036073.8A patent/CN109312307A/zh active Pending
- 2017-04-07 JP JP2018552760A patent/JP7049261B2/ja active Active
-
2022
- 2022-02-09 JP JP2022018520A patent/JP2022058939A/ja active Pending
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