JP2019196333A - Periodontal disease inhibitor and oral composition using the same - Google Patents

Abstract

To provide periodontal disease inhibitors and oral compositions that can be used in the prevention of intraoral diseases in the progression of aging and can be satisfied overall in terms of effectiveness, safety, feeling of use, and the like.SOLUTION: Provided is a periodontal disease inhibitor containing a pressed solution, extract or distillate of a plant belonging to Pinaceae Abies genus as an active ingredient, and an oral composition containing the above-mentioned compressed liquid, extract or distillate and an oral composition base.SELECTED DRAWING: None

Description

  The present invention relates to a novel periodontal disease bacteria inhibitor and oral composition.

  In the human oral cavity, a very wide variety of bacteria exceeding 500 to 700 species has been found, and an aggregate, that is, a bacterial flora is constantly formed on the mucosal surface. This intraoral flora is maintained in a balance by complex interactions, and has a function of preventing invasion infection, that is, imparting resistance by antagonizing foreign bacteria.

  However, on the other hand, there are periodontal bacteria that cause infections in the oral cavity, and the periodontal disease caused by these bacteria is said to be the most common oral infection.

  Periodontal diseases caused by periodontal disease bacteria include gingivitis, periodontitis and occlusal trauma. In addition, it is known that periodontal disease becomes chronic and causes infections such as respiratory diseases, heart diseases, diabetes, and premature birth due to periodontal disease bacteria.

  The main cause of periodontal disease is bacteria in the plaque that accumulate in the periodontal pocket. Usually, Gram-positive bacteria occupy most of the periodontal pockets of healthy individuals, but as periodontal disease progresses, such as Porphylomonas gingivalis and Fusobacterium nucleatum, It is known that gram-negative bacteria called periodontal disease bacteria increase.

  Numerous studies have been conducted on the suppression of periodontal disease bacteria, and toothpastes and gargles containing antibacterial substances effective against these bacteria have been developed. For example, as an antibacterial agent against periodontal disease bacteria, an active ingredient containing cocoa contained in cacao mass (Patent Document 1), an anti-cariogenic agent such as Koganebana extract (Patent Document 2), allyl isothiocyanate Anti-periodontal disease agent (patent document 3) containing isothiocyanate compound such as mint essential oil or its essential oil component, anti-periodontal disease agent (patent document 5) containing cinnamon extract as an active ingredient, thyme extraction Periodontal disease prevention composition (patent document 6) etc. containing a thing, and 1 type, or 2 or more types of essential oil and an oleoresin group are known.

  However, there are few things that have been developed so far in terms of effectiveness, safety, usability, etc., and prevention of oral diseases is an important issue as aging progresses. Therefore, there is a demand for the development of superior periodontal disease bacteria inhibitors.

  On the other hand, there are reports that sesquiterpenes contained in conifers suppress the antibacterial effect against mutans bacteria and biofilm formation (Patent Document 4 and Non-Patent Document 1). In addition, it has been reported that 1,8-cineole is effective against mutans (Non-Patent Document 2). However, these are not for periodontal bacteria and do not suggest application to periodontal bacteria.

International Publication No. 2003/99304 Pamphlet JP 2001-97835 A JP 2007-308378 A JP 2012-229191 A Japanese Patent Laid-Open No. 4-29933 Japanese Patent Laid-Open No. 3-255031

2017 Annual Meeting of the 44th Annual Meeting of the Japanese Bar Metal Protection Society Abstract P185 Abstracts of the 57th Annual Meeting of the Japanese Society of Chemotherapy P164

  Accordingly, an object of the present invention is to provide a highly practical periodontal disease inhibitor and a composition for oral cavity using the same, from the viewpoints of effectiveness, safety, and feeling of use.

  In order to solve the above problems, the present inventors have been searching for an antibacterial component in natural products, and found that a substance having an effect of significantly suppressing periodontal disease bacteria exists in the branches and leaves of a specific conifer. The headline and the present invention were completed.

  That is, this invention is a periodontal disease microbe inhibitor characterized by containing the pressing liquid, extract, or distillate of the plant which belongs to the pine family Fir genus as an active ingredient.

  Moreover, this invention is a composition for oral cavity characterized by containing the pressing liquid of the plant which belongs to the pine family Fir genus as an active ingredient, an extract or a distillate, and an oral composition base.

  Furthermore, the present invention is a method for inhibiting periodontal disease bacteria, characterized by applying the above-mentioned periodontal disease inhibitor or oral composition to the oral cavity.

  According to the periodontal disease inhibitor of the present invention, the growth of periodontal disease bacteria in the oral cavity can be remarkably suppressed, which is effective in the treatment and prevention of periodontal disease.

  Moreover, since the composition for oral cavity of this invention is a dosage form of the form used on a daily basis, since it contains the periodontal disease bacteria suppression component, it can prevent periodontal disease easily without being especially conscious. Is.

It is a figure which shows typically the structure of the microwave distillation apparatus used in order to obtain the distillate of the plant which belongs to the pine family Fir genus used as an active ingredient of the periodontal disease bacteria inhibitor of this invention.

  The active ingredient of the agent for inhibiting periodontal disease of the present invention is a pressed solution, extract or distillate (hereinafter sometimes referred to as “periodontal disease inhibiting ingredient”) of a plant belonging to the genus Pinaceae.

  Plants belonging to the pine family Fir genus, which is a raw material for this periodontal disease inhibitory ingredient, are Todomatsu, Fir, Virgin Fir, Silaviso, Greater Silviso, Silabe, Balsam Fur, Mitsumine Fir, White Fur, Amabilis Fur, Aotodomatsu, California Red Fur, Grand Fur, noble fur and the like. Of these, Todomatsu is preferably used because of its excellent effect on periodontal disease bacteria.

  In preparing the periodontal disease inhibitory component of the present invention, all or part of the plant belonging to the genus Firaceae (for example, fruit, seed, leaf, bark, rhizome, flower, etc.) is used. Of these, the leaf portion is preferably used because of the effective use of trees that are thinned and pruned and the high suppression effect. The leaf portion may be used as it is, but is preferably used after being pulverized and crushed by a pulverizer or a crusher.

  The said periodontal disease suppression component is obtained as a squeezed liquid, an extract, or a distillate from the whole plant part of the plant which belongs to Pinaceae fir genus, or its part. Among these, the production of the juice can be performed using a known press. In addition, although the extract can be produced according to a conventional method, it is preferable to use a polar solvent as the extraction solvent, specifically, water, ethanol, methanol, butyl alcohol, ethylene glycol, propylene glycol, glycerin. Examples thereof include alcohol solvents such as chloroform; halogen-containing solvents such as chloroform; ether solvents such as dioxane; carbonyl solvents such as acetone and ethyl acetate, and mixtures thereof. The distillate can be obtained by atmospheric distillation, vacuum distillation, steam distillation or the like.

  Of these, the distillate is preferable as the active ingredient of the periodontal disease inhibitor of the present invention, and those obtained by heating and distillation under reduced pressure are particularly preferable. This product contains monoterpenes at a high concentration and has a refreshing fragrance without requiring time and effort for concentration, purification, etc., so it has a high palatability and a high periodontal disease inhibitory effect. Therefore, it is preferable.

  The heating for distillation may be heating by a general heater or the like, but heating by microwave is preferable from the viewpoint of heating efficiency, and heating by microwave irradiation is particularly preferable without adding water. By irradiating microwaves, water molecules contained in plants are directly heated to generate water vapor, which acts as a mobile phase and distills volatile components in the plants. It is considered to include a vacuum distillation element depending on the boiling point of the component and a steam distillation element.

  Such a distillate by microwave irradiation can be obtained, for example, by using the apparatus shown in FIG. In the figure, 1 is a microwave distillation apparatus, 2 is a distillation tank, 3 is a microwave heating apparatus, 4 is a stirring splash, 5 is an airflow inflow pipe, 6 is a distillate outflow pipe, 7 is a cooling apparatus, and 8 is heating control. Device, 9 is a pressure reducing pump, 10 is a pressure regulating valve, 11 is a pressure control device, 12 is an object to be distilled, and 13 is a distillate.

  In this apparatus 1, a plant belonging to the genus Pinaceae, which is a raw material to be distilled 12, is placed in a distillation tank 2, and stirred with a stirring splash 4, and a microwave heating apparatus provided on the upper surface of the distillation tank 2. Microwaves are emitted from 3 to heat the raw material. The distillation tank 2 communicates with the air flow inlet 5 and the distillate outflow pipe 6. The airflow inlet pipe 5 is for introducing an inert gas such as air or nitrogen gas into the distillation tank 2, and this airflow is introduced from the lower part of the distillation tank 2. The distillate outflow pipe 6 leads the distillate from the raw material to the outside from the upper part of the distillation tank 2.

  The inside of the distillation tank 2 is such that the temperature and pressure are measured by a temperature sensor and a pressure sensor (both not shown) attached thereto, and a heating control device 8, a pressure control device 11, a pressure regulating valve 10 are adjusted respectively.

  The gaseous distillate flowing out from the distillation tank 2 through the distillate outflow pipe 6 is replaced with a liquid by the cooling device 7 and obtained as a distillate 13. The distillate 13 includes an oily fraction 13a and an aqueous fraction 13b. Of these, the oily fraction 13a is preferably used.

  In the distillation, the pressure in the distillation tank 2 may be 3 to 95 kilopascals, preferably 3 to 40 kilopascals, more preferably 3 to 20 kilopascals, and the steam temperature at that time is 40 ° C. To 100 ° C. When the pressure is 3 kilopascals or less, the increase in vapor pressure of volatile components in the plant is suppressed, and the vacuum distillation component due to the boiling point of each component is the main component from the steam distillation component. Therefore, it is not preferable in that a component having a boiling point higher than that of water is not efficiently extracted, and that a high boiling component having a low repellent efficiency and inferior in aroma preference is extracted. Moreover, since the temperature of a raw material becomes high at 95 kilopascals or more, it is not preferable at the point that an energy loss is large and the oxidation of a raw material will be accelerated | stimulated.

  The distillation time may be about 0.2 to 8 hours, preferably about 0.4 to 6 hours. If it is 0.2 hours or less, a large amount of unextracted components remain in the plant, and if it is 8 hours or more, the raw material becomes nearly dry, which is not preferable in that the extraction efficiency decreases.

  Furthermore, the gas introduced into the distillation tank 2 may be air, but an inert gas such as nitrogen gas, helium gas, or argon gas is preferable, and the flow rate per minute is the distillation tank 2. It may be about 0.001 to 0.1 times the capacity.

  As the periodontal disease inhibitory component of the present invention, the juice, extract or distillate of the plant belonging to the genus Pinaceae obtained as described above can be used as it is, but if necessary, by a conventional method. Further, it may be concentrated or purified before use.

  In the periodontal disease inhibitor of the present invention, the above-mentioned periodontal disease bacteria-inhibiting component may be used in that state, but may further be used in combination with an appropriate solvent.

  Examples of the solvent used in the periodontal disease inhibitor of the present invention include water, ethanol, isopropyl alcohol, propyl alcohol and other alcohol solvents, propylene glycol, ethylene glycol and other glycol solvents, ethylene glycol monomethyl ether, propylene Examples include glycol ether solvents such as glycol monomethyl ether, propylene alcohol monoethyl ether, and 3-methoxy-3-methyl-1-butanol.

  In the case of using a solvent as a periodontal disease inhibitor, the amount of the periodontal disease inhibitor component is 0.01% by mass (hereinafter simply referred to as “%”) to about 99%, preferably Is between 0.1% and 20%.

  The compounding amount of the periodontal disease suppressing component in the periodontal disease bacterial inhibitor of the present invention is not particularly limited as long as the effect can be confirmed, but for example, a preferable amount when using a Todomatsu extract is generally used. Is set in the range of 0.001 mg / g to 200 mg / g in the preparation, and preferably in the range of 0.05 mg / g to 20 mg / g.

  In addition, the other periodontal pathogen control component of this invention may be mix | blended with the periodontal pathogen control agent of this invention, and, thereby, the outstanding periodontal pathogen control effect can be exhibited. Examples of these other periodontal disease inhibitory ingredients include cetylpyridinium chloride, isopropylmethylphenol, lauroyl sarcosine salt, chlorhexidine, tranexamic acid, tetracycline, minocycline, lemongrass mastick oil, chamomile tincture, latania tincture, hinokitiol, sandalwood , Cassia, Cinnamon, Guayac, Patchouli Essential Oil, Gallic Acid, Catechin, Red Ganoderma, Black Ganoderma, Twilight Leaf, Green Tea, Artemisia, Karin, Ginger Pear, Gymnema, Rooibos Tea, Hawthorn, Turmeric, Lacanca, Silymarin, Eggplant , Purple brown rice, eleuterokock, moon peach leaf, dokudami, large coral, ganoderma, garlic, chimpanzee, licorice, chamomile, chasensou, kyoto, keihi, fennel, hops, valerian, gon, shikon, sage, ginkgo Bamboo, mulberry bark, litchi, Akashi ginger, mention may be made of rice extract or the like, can be used by mixing one or two or more of these.

  The periodontal disease inhibitor of this invention can also be made into the composition for oral cavity combined with the well-known oral composition base. The oral composition may be in any form such as liquid, solid, powder, paste, etc., and an optimal dosage form can be arbitrarily selected for carrying out the present invention.

  Specific oral compositions include dentifrices, toothpastes, toothpastes, liquid toothpastes, mouthwashes, mouth fresheners, gargle tablets, denture cleaners, mouthwashes, troches, chewing gums. And various dosage forms.

  As the oral composition base used for the preparation of the oral composition of the present invention, known abrasives, surfactants, and thickeners as long as they are of the kind and range that do not impair the effects of the present invention. Ingredients and additives such as sweeteners can be mentioned, and these can be selected and used in combination.

  Among these, for example, silica-based abrasives such as silicic anhydride, aluminum hydroxide, aluminum oxide, aluminosilicate, calcium hydrogen phosphate, dicalcium phosphate, insoluble sodium metaphosphate, insoluble potassium metaphosphate, titanium oxide A synthetic resin or the like is preferably used as necessary. These abrasives can be compounded by mixing one or more kinds.

  Examples of the surfactant include water-soluble salts of higher alkyl sulfates having 8 to 18 carbon atoms in the alkyl group, such as sodium lauryl sulfate and sodium myristyl sulfate, sodium lauryl monoglyceride sulfonate, sodium coconut monoglyceride sulfonate, and the like. Of fatty acid groups such as higher fatty acid monoglyceride sulfonic acid having 10 to 18 carbon atoms, olefin sulfonic acid, paraffin sulfonic acid, other anionic activators, stearyl monoglyceride, sucrose mono and dilaurate Sucrose fatty acid ester having 12 to 18 carbon atoms, lactose fatty acid ester, lactitol fatty acid ester, maltitol fatty acid ester, stearic acid monoglyceride, polyoxyethylene sorbitan monolaure Nonionic activators such as polyoxyethylene hydrogenated castor oil, sorbitan monostearate condensate with about 60 mol of ethylene glycol added, polymer of ethylene oxide and propylene oxide, and derivatives such as polyoxyethylene polyoxypropylene monolauryl ester In addition, surfactants such as amphoteric surfactants such as betaine type and amino acid type can be used, and one or more of these can be mixed and blended.

  Furthermore, as the thickener, for example, carrageenan, cellulose derivatives such as sodium carboxymethylcellulose, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, sodium carboxymethylhydroxyethylcellulose, alkali metal alginates such as sodium alginate, propylene glycol alginate, xanthan gum, tragacanth gum, Gums such as karaya gum and gum arabic, synthetic thickeners such as polyvinyl alcohol, sodium polyacrylate, carboxyvinyl polymer, polyvinylpyrrolidone, etc., inorganic thickeners such as gelling silica, gelling aluminum silica, beegum and laponite Etc., and one or more of these can be blended.

  Furthermore, as a thickener, 1 or more types, such as sorbit, glycerin, ethylene glycol, propylene glycol, 1, 3- butylene glycol, polyethylene glycol, polypropylene glycol, xylite, maltite, lactite, can be mix | blended, for example.

  Still further, as sweeteners, for example, saccharin sodium, stepioside, xylitol, neohesperidyl dihydrochalcone, glycyrrhizin, perilartine, thaumatin, aspartylphenylalanine methyl ester, p-methoxycinnamic aldehyde, sodium cyclamate, etc. For example, p-hydroxymethyl benzoic acid, p-hydroxyethyl benzoic acid, p-hydroxypropyl benzoic acid, p-hydroxybutyl benzoic acid, sodium benzoate, lower fatty acid monoglyceride, etc. Green oil, spearmint oil, peppermint oil, sassafras oil, clove oil, eucalyptus oil can be listed, and these are blended as needed Rukoto can.

  In addition, other components such as gelatin, peptone, albumin, brightener, silicone, pigment, fluorides such as sodium fluoride, sodium monofluorophosphate, tin fluoride, epsilon aminocaproic acid, dextranase, Enzymes such as protease, mutanase, lysozyme, lytic enzyme, lytechenzyme, chlorhexidine salts, dihydrocholesterol, glycyrrhetin salts, glycyrrhetinic acid, caropeptides, vitamins, antibacterial agents, and other medicinal components such as hyposensitive agents such as potassium nitrate One or more kinds may be blended.

  The pH of the composition for oral cavity of the present invention is not particularly limited as long as it is in a range that does not cause safety problems in the oral cavity and the human body, but is preferably pH 6-9, more preferably 7-9. is there. If necessary, acetic acid, hydrochloric acid, sulfuric acid, nitric acid, citric acid, phosphoric acid, sodium hydroxide, potassium hydroxide, sodium acetate, sodium carbonate, sodium citrate, sodium hydrogen citrate, phosphorus An appropriate amount of sodium acid, sodium hydrogen phosphate, or the like can be blended.

  An example of a preferred embodiment of the oral composition of the present invention is a toothpaste dosage form, and examples of the oral composition base include calcium hydrogen phosphate, glycerin and water.

  Moreover, as an example of another preferable embodiment, what contains ethanol and water as an oral composition base can be mentioned as a mouthwash dosage form.

  Furthermore, as an example of another preferable embodiment, it is possible to cite a humectant dosage form containing oral glycerin and water as the oral composition base.

  The periodontal disease inhibitor described above may be prepared by, for example, including a liquid preparation containing an effective amount of a periodontal disease inhibitor component in the mouth about 1 to 3 times a day, and rinsing and rinsing. Moreover, the composition for oral cavity should just use the composition containing an effective amount of a periodontal disease suppression component by the normal usage corresponding to the dosage form.

  Hereinafter, the present invention will be described in more detail with reference to Production Examples, Examples and Formulation Examples, but the present invention is not limited thereto.

Manufacturing example 1
Using Todomatsu leaves as a raw material, Todomatsu essential oil was obtained as follows. That is, about 50 kg of Todomatsu leaves pulverized with a crushing crusher (manufactured by KYB Seisakusho) is put into a distillation tank of a microwave distillation apparatus as shown in FIG. The oil was kept under reduced pressure of 20 KPa, and the essential oil was distilled by microwave irradiation for 1 hour (vapor temperature is about 67 ° C.). The amount of the essential oil obtained was 180 mL, and the ratio of the essential oil to the input sample was 0.34%.

Manufacturing example 2
Using the same Todomatsu leaves as in Production Example 1, Todomatsu essential oil was obtained by the following steam distillation method. That is, about 101 g of Todomatsu leaves pulverized by a squeezing type pulverizer (manufactured by KYB Seisakusho) was placed in a Pyrex (registered trademark) glass flask, 5 to 8 times the amount of water was added, and then the flask was placed in a hot water bath. Heated to ~ 100 ° C and boiled. The essential oil sampling tube was filled with water up to the baseline before heating. When the essential oil was distilled by continuing to boil for 6 hours, 0.8 mL of the essential oil was obtained. The ratio of essential oil to the input sample was 0.79%.

Example 1
Preparation of periodontal disease inhibitor:
Each component was taken in the ratio shown in the prescription of Table 1 below, and these were mixed to prepare a periodontal disease inhibitor of the present invention. In addition, as comparative products 1-3, instead of Todomatsu essential oil, eucalyptus oil, tea tree oil or tea extract, which is generally said to be effective against periodontal disease bacteria, is further added as a control product. A surfactant dissolved in a water-ethanol mixture (control product 1) and a water-ethanol mixture only (control product 2) were prepared.

<Treatment>

Example 2
Periodontal disease suppression effect test:
Using 10 g of each sample prepared with the composition of Example 1, and using the following test method, the oral cavity microorganism containing the periodontal disease inhibitor of the present invention (product of the present invention), each comparative product, and the control product of periodontal disease bacteria The antibacterial action against was investigated.

<Test method>
To the wells of the 96-well plate, the specified media corresponding to the test microorganisms were taken, and the solutions of the present invention product, each comparative product, and each control product diluted to 31.25 to 4000 ppm were added thereto.

  Subsequently, the precultured bacteria of Table 2 below were inoculated into each well, and cultured for 48 hours at the culture temperature and culture conditions corresponding to the test microorganisms. After completion of the culture, the presence or absence of bacterial growth was visually confirmed, and the minimum concentration at which no growth was observed was defined as the minimum growth inhibitory concentration (MIC). The results are shown in Table 3.

  From the above results, it was confirmed that the product of the present invention has a remarkable inhibitory action against two periodontal bacteria (P. gingivalis and F. nucleatum). This was stronger antibacterial activity than eucalyptus oil, tea tree oil, and tea extract, which have been conventionally effective for periodontal disease bacteria. On the other hand, the product of the present invention was not effective against fungi such as bait-causing bacteria (gram-positive bacteria) and Candida.

Product example 1
Toothpaste:
(Component) Mass%
1. Calcium hydrogen phosphate 40.0
2. Glycerin 20.0
3. Carrageenan 1.0
4. Sodium lauryl sulfate 1.5
5. Saccharin 0.2
6. Todomatsu Extract from Production Example 1 1.0
7. Chlorhexidine digluconate 0.1
8. Preservative (Butyl paraoxybenzoate) Appropriate amount 9. Fragrance (Lemon water) Appropriate amount 10. Colorant Appropriate amount 11. Purified water balance

Product example 2
Toothpaste
(Component) Mass%
1. Calcium hydrogen phosphate 30.0
2. Glycerin 20.0
3. Cetylpyridinium chloride 0.2
4. Sodium lauryl sulfate 1.5
5. Saccharin sodium 0.1
6. Todomatsu Extract of Production Example 2 2.0
7. Sodium monofluorophosphate 4.0
8. Preservative (methylparaben) appropriate amount 9. Perfume (orange water) appropriate amount 10. Colorant appropriate amount 11. Purified water balance

Product example 3
Mouthwash:
(Component) Mass%
1. Ethanol 20.0
2. Sodium saccharin 0.1
3. Sodium fluoride 0.1
4. Ascorbic acid 0.1
5. Sodium pyrithione 0.5
6. Benzethonium chloride 0.1
7. Surfactant (sucrose fatty acid ester) 3.0
8. Todomatsu Extract from Production Example 1 1.0
9. Preservative (Methylparaben) Appropriate amount 10. Perfume (Mint water) Appropriate amount 11. Colorant Appropriate amount 12. Purified water balance

Product example 4
Mouth freshener:
(Component) Mass%
1. Ethanol 20.0
2. Glycerin 15.0
3. Polyoxyethylene hydrogenated castor oil 1.0
4. Saccharin 0.2
5. Chlorohexidine 0.01
6. Todomatsu Extract from Production Example 1 1.0
7. Surfactant (Neugen HC-600) 3.0
8. Preservative (methyl paraben) appropriate amount 9. Colorant appropriate amount 10. Purified water balance

Product example 5
Chewing gum:
(Component) Mass%
1. Plate gum base 24.0
2. Minamata 13.0
3. Powdered sugar 60.0
4. Todomatsu Extract from Production Example 1 1.0
5. Preservative (methyl paraben) appropriate amount 6. Fragrance (lemon water) appropriate amount 7. Purified water balance

Product example 6
Candy:
(Component) Mass%
1. Granulated sugar 45.0
2. Todomatsu Extract from Production Example 1 1.0
3. Preservative (methyl paraben) appropriate amount 4. Fragrance (apple water) appropriate amount 5. Purified water balance

Product example 7
Oral moisturizer:
(Component) Mass%
1. Glycerin 30.0
2. Todomatsu Extract from Production Example 1 1.0
3. Kitansan gum appropriate amount 4. Xylitol appropriate amount Perfume (Mint) Appropriate amount 6. Purified water balance

  The present invention is a periodontal fungus inhibitor using a substance that remarkably suppresses periodontal disease bacteria in the branches and leaves of a specific conifer and a composition for oral cavity, but since the origin of the ingredients is natural products, It is highly safe.

  And since this component is highly effective against various periodontal pathogens, it can be widely used as a quasi-drug, daily toothpaste, mouthwash, mouthwash, etc. is there.

DESCRIPTION OF SYMBOLS 1 ...... Microwave distillation apparatus 2 ... Distillation tank 3 ...... Microwave heating apparatus 4 ...... Stirring splash 5 ...... Airflow inflow pipe 6 ...... Distillate outflow pipe 7 ...... Cooling apparatus 8 ...... Heating control apparatus 9 …… Pressure reducing pump 10…… Pressure regulating valve 11…… Pressure control device 12…… Distillation object

Claims (13)

  A periodontal fungus inhibitor characterized by containing a pressed solution, extract or distillate of a plant belonging to the genus Piraceae as an active ingredient.   The periodontal fungus inhibitor according to claim 1, wherein the pressed solution, extract or distillate of a plant belonging to the genus Pinaceae is a pressed solution, extract or distillate of a plant belonging to the genus Pinaceae.   The active ingredient is a distillate of a plant belonging to the genus Piraceae, and is a distillate obtained by performing distillation by heating a plant belonging to the genus Piraceae under reduced pressure. Periodontal disease bacteria inhibitor.   The periodontal disease bacteria inhibitor of Claim 3 whose active ingredient is an oily fraction of the distillate obtained by heating and distilling the plant which belongs to Pinaceae fir genus under reduced pressure.   The periodontal disease bacteria inhibitor of Claim 3 or 4 whose heating is performed by microwave heating.   The plant belonging to the genus Piraceae is Todomatsu, The periodontal disease bacteria inhibitor according to any one of claims 1 to 5.   The periodontopathic fungus according to any one of claims 1 to 6, which has an inhibitory action on at least one of the periodontal pathogens of Porphylomonas gingivalis or Fusobacterium nucleatum. Inhibitor.   A composition for oral cavity, comprising a pressed solution, extract or distillate of a plant belonging to the genus Firaceae as an active ingredient, and an oral composition base.   The composition for oral cavity according to claim 8, which is a toothpaste dosage form and contains calcium hydrogen phosphate, glycerin and water as an oral composition base.   The composition for oral cavity according to claim 8, which is a mouthwash dosage form and contains ethanol and water as an oral composition base.   The composition for oral cavity of Claim 8 which is a dosage form of an intraoral moisturizer and contains glycerin and water as an oral composition base.   A method for inhibiting periodontal disease bacteria, comprising applying the periodontal disease inhibitor according to any one of claims 1 to 7 to the oral cavity. A method for suppressing periodontal disease bacteria, comprising applying the composition for oral cavity according to any one of claims 8 to 11 to the oral cavity.

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