JP2016094354A - Anti-inflammatory external preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an anti-inflammatory external preparation which can give the motivation for exercise to achieve exercise continuously, and which can forestall localized inflammation caused by exercise by being applied before the exercise.SOLUTION: The invention provides an anti-inflammatory external preparation which can give the motivation for exercise and which can forestall localized inflammation caused by exercise by being applied before the exercise, the external preparation containing an oil-extracted fraction or water-extracted fraction extracted from Pinaceae Abies branches and/or leaves as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to an anti-inflammatory external preparation, and more specifically, by containing an oily extract fraction or an aqueous extract fraction as an active ingredient, it can provide motivation for exercise and can be applied before exercise. The present invention relates to an anti-inflammatory external preparation that can suppress local inflammation caused by exercise.

  It has been a long time since lifestyle-related diseases such as hypertension, diabetes, dyslipidemia, and locomotive syndrome have been regarded as problems. Everyone admits that continuous exercise is necessary to prevent these lifestyle-related diseases. On the other hand, everyone also realizes that continuous exercise is difficult.

  There are a number of techniques that motivate the athletic behavior itself for the purpose of achieving continuous exercise. For example, Patent Document 4 discloses an exercise motivation system that enables continuation of walking by distributing music that matches a user's preference and exercise amount target, and Patent Document 5 detects and stores a plurality of user's exercise events. In addition, it provides a motion promoting robot designed to output sound at a timing that matches each motion and to move in response to the motion.

  These are technologies based on the hypothesis that the user can always exercise in response to motivation. However, in fact, exercise has created new factors that inhibit continuous exercise, and local inflammation is a particularly serious factor. Local inflammation causes pain such as muscle pain and inhibits continuous exercise as mental stress as well as physical stress. Thus, in order to be able to continue exercise, it is necessary not only to provide motivation for the aforementioned exercise but also to provide local inflammatory care to maintain optimal physical conditions.

  Although local inflammatory care can be performed during and after exercise, it is generally performed after exercise, and non-steroidal anti-inflammatory external preparations are widely used. Non-steroidal anti-inflammatory topical drugs, which are pharmaceuticals, are generally used after recognizing local inflammation, and inevitably cannot suppress pain caused by exercise, and physical and mental stress caused by pain Because of this, continuous exercise is difficult.

  In recent years, anti-inflammatory external preparations derived from natural products different from the non-steroidal anti-inflammatory external preparations have been widely used. For example, Patent Document 3 discloses a skin external preparation characterized by containing a mixed essential oil composed of mint leaf oil, lemon oil, Italian cedar oil, Labandula hybrida oil, and Kitus sura danifel oil. An anti-inflammatory agent containing Camellia plant extract is provided in Patent Document 5, and an external preparation for skin characterized by containing an extract of Pinaceae Larix plant in Patent Document 5 is provided. Yes. However, none of these have yet suppressed local inflammation caused by exercise. Then, the anti-inflammatory external preparation which can suppress the local inflammation which arises by exercise | movement by applying before exercise | movement was desired.

  In view of the above, an anti-inflammatory external preparation that can provide motivation for exercise and can suppress local inflammation caused by exercise by applying it before exercise in order to realize continuous exercise. Development was desired.

JP 2003-305146 A JP 2013-090758 A Japanese Patent Laying-Open No. 2005-075827 JP 2014-080409 A JP2013-010698A

  The present invention has been made in view of the above-described situation, and its purpose is to provide motivation for exercise in order to realize continuous exercise, and to suppress local inflammation caused by exercise in advance. It is to provide an anti-inflammatory external preparation that can be used.

As a result of diligent research to solve the above-mentioned problems, the present inventors include an oily extract or an aqueous extract fraction extracted from branches and / or leaves of the pine family, as an active ingredient in an external preparation. Thus, it was found that an anti-inflammatory external preparation that exhibits a high anti-inflammatory effect and a motivational addition effect to exercise can be obtained, and based on this finding, the present invention has been completed.
That is, the present invention is as shown in the following (1) to (7).
(1) An anti-inflammatory external preparation characterized by containing an oily extract fraction or an aqueous extract fraction extracted from branches and / or leaves of a plant of the genus Piraceae as an active ingredient that exhibits an anti-inflammatory effect.
(2) The anti-inflammatory external preparation according to (1), wherein the plant belonging to the genus Fir is a Todomatsu.
(3) The anti-inflammatory external preparation according to (1) or (2), wherein the content of the oily extract fraction is 0.1 to 10% by mass in the base material.
(4) The anti-inflammatory external preparation according to (1) or (2) above, wherein the content of the aqueous extract fraction is 25 to 99.9% by mass in the base material.
(5) The anti-inflammatory external preparation according to any one of (1) to (4), wherein the shape is gel or stick.
(6) The anti-inflammatory external preparation according to any one of (1) to (5), which adds motivation to exercise.

  As described above, the present invention adds a high anti-inflammatory effect and exercise to an external preparation by adding an oily extract or aqueous extract extracted from branches and / or leaves of Pinaceae as an active ingredient. It is possible to provide an anti-inflammatory external preparation that exerts a motivational additional effect.

An example of the structure of the microwave distillation apparatus used in order to obtain the distillate of the plant which belongs to the pine family Fir genus used as an active ingredient of the anti-inflammatory external preparation of this invention is shown typically.

  Hereinafter, the anti-inflammatory external preparation of the present invention will be described in detail. Note that the examples described in the present specification do not particularly limit the present invention.

  The “anti-inflammatory effect” of the present invention means an “edema suppression rate” calculated from the “edema rate” of each test topical agent application group, and those having an edema suppression rate of 15% or more have an anti-inflammatory effect. It was judged. “Edema rate” refers to the edema volume of the limb volume before the edema, that is, edema when the limb volume is measured by dropping 100 g of the weight for the upper pan balance from the height of 1 m on the right hind limb of the rat. It means the ratio of the difference between the hind limb volume and the edema-induced paw volume. In addition, “edema suppression rate” refers to a test for the edema rate of the control topical agent application group that does not contain an oily extract or aqueous extract extracted from branches and / or leaves of a plant of the genus Firaceae. It means the ratio of the difference between the edema rate reduced by the application of the external agent, that is, the edema rate of the control external agent application group and the test external agent application group.

  The “motivation addition effect” for the exercise of the present invention means “motivation addition rate” of each test topical agent application group, and the motivation addition rate is 60% (3 out of 5) or more. It was judged that there was a motivational additional effect. “Motivational addition rate” means the rate at which a subject wants to exercise due to olfactory stimulation of an oily extract or aqueous extract extracted from branches and / or leaves of a plant of the genus Firaceae.

The active ingredient used in the anti-inflammatory topical preparation of the present invention is an oily extract or an aqueous extract fraction extracted from branches and / or leaves of a plant of the genus Piraceae.
Plants of the genus Firaceae include, for example, Todomatsu, Fir, Virgin Fir, Silaviso, Greater Silvia, Silabe, Balsam Fir, Mitsune Fir, White Fur, Amabilis Fur, Aoto Domatsu, California Red Fur, Grand Fur and Noble Fur, etc. Todomatsu is preferable.

  As a method for obtaining an oily extract or an aqueous extract from branches and / or leaves of a tree of the genus Piraceae, it can be carried out according to conventional methods such as solvent extraction and distillation. The extraction solvent is preferably a polar solvent, for example, alcohol solvents such as water, ethanol, methanol, butyl alcohol, ethylene glycol, propylene glycol and glycerine; halogen-containing solvents such as chloroform; ether solvents such as dioxane; acetone And carbonyl solvents such as ethyl acetate, and mixed solutions thereof. The distillate can be obtained by atmospheric distillation, vacuum distillation, steam distillation or the like.

  Of these, distillates are preferred, and those obtained by distillation under reduced pressure are particularly preferred. Heating may be performed by a heater, but microwave heating is preferable, and it is particularly preferable to irradiate microwaves without adding water. By irradiating microwaves, water molecules contained in plants are directly heated to generate water vapor, which acts as a mobile phase and distills volatile components in the plants. It is considered to include a vacuum distillation element depending on the boiling point of the component and a steam distillation element.

Such a distillate can be obtained, for example, by using the apparatus shown in FIG.
In this apparatus 1, a branch and / or leaf of a pine family, which is a raw material to be distilled 12, is placed in a distillation tank 2 and stirred with a stirring splash 4 while being provided on the upper surface of the distillation tank 2. Microwaves are emitted from the heating device 3 to heat the raw material. The distillation tank 2 communicates with the air flow inlet 5 and the distillate outflow pipe 6. The airflow inlet pipe 5 introduces an inert gas such as air or nitrogen gas into the reaction tank 2, and this airflow is introduced from the lower part of the reaction tank 2. The distillate outflow pipe 6 leads the distillate from the raw material to the outside from the upper part of the reaction tank 2.
The inside of the reaction vessel 2 can be measured for temperature and pressure by a temperature sensor and a pressure sensor (both not shown) attached thereto, and can be adjusted via the heating control device 8, the pressure control device 11, and the pressure regulating valve 10, respectively. .
Further, the gaseous distillate flowing out from the distillation tank 2 through the distillate outflow pipe 6 is replaced with a liquid by the cooling device 7 to obtain a distillate 13. The distillate 13 includes an oily fraction 13a and an aqueous fraction 13b, and among these, the aqueous fraction 13b is preferably used.

  In the distillation, the pressure in the distillation tank 2 may be 10 to 95 kPa, preferably 15 to 60 kPa, more preferably about 20 to 40 kPa, and the vapor temperature at that time is 40 ° C to 100 ° C. When the pressure is 10 kPa or less, the increase in the vapor pressure of the volatile components in the plant is suppressed, and the vacuum distillation component due to the boiling point of each component is mainly from the steam distillation component, and the components flow out in order from the lowest boiling point. Therefore, it is not preferable in that extraction of components having a boiling point higher than that of water cannot be performed efficiently. Moreover, if it is 95 kPa or more, since the temperature of a raw material becomes high, an energy loss is large and it is not preferable at the point which oxidizes raw material. The distillation time may be about 0.2 to 8 hours, preferably about 0.4 to 6 hours. If it is 0.2 hours or less, a large amount of unextracted components remain in the plant, and if it is 8 hours or more, the raw material becomes nearly dry, which is not preferable in that the extraction efficiency decreases.

The oily extract fraction or aqueous extract fraction obtained by such a method is preferable because it contains a high concentration of monoterpene and can give a refreshing aroma component.
Further, the gas introduced into the distillation tank 2 may be air, but inert gas such as nitrogen gas, helium gas and argon gas is preferable, and the flow rate per minute is the distillation tank 2. About 0.001 to 0.1 times the capacity.

  The content of the oily extract fraction used in the anti-inflammatory external preparation of the present invention is preferably in the range of 0.1 to 20% by mass, more preferably 1.0 to 10% by mass of the total content of the external preparation. It is. If the content of the oily extracted fraction is less than 0.1% by mass, the motivational effect on exercise and the anti-inflammatory effect cannot be obtained, which is not preferable. Moreover, when there is more content of an oily extract fraction than 20 mass%, since there exists a possibility of causing skin irritation and skin sensitization, it is not preferable.

  The content of the aqueous extract fraction used in the anti-inflammatory external preparation of the present invention is preferably in the range of 25 to 99.9 mass%, more preferably 30 to 98 mass% of the total content of the external preparation. . If the content of the oily extract is less than 25% by mass, the motivational effect on exercise and the anti-inflammatory effect cannot be obtained, which is not preferable. Moreover, when there is more content of an aqueous extraction fraction than 99.9 mass%, since it is difficult to comprise an external preparation, it is unpreferable.

  Examples of the dosage form of the anti-inflammatory external preparation of the present invention include ointment, gel, stick, cream, tape, pap and lotion. When directly applying to the skin with bare hands, It is preferable that the gel form is sticky because it is not sticky, and a stick form that can withstand the coating by integrating the container and the external preparation when the container is applied to the skin.

  When the anti-inflammatory external preparation of the present invention is in the form of a gel, an oily extract fraction or an aqueous extract fraction, a gelling agent and water are essential components.

  Examples of the gelling agent used in the gel-like anti-inflammatory external preparation include xanthan gum, carboxyvinyl polymer, pectin, polyethylene, beeswax, barafine, sodium polyacrylate, etc., and these may be used alone or in combination of two or more. Can do. The content of the gelling agent is preferably in the range of 0.01 to 10% by mass, more preferably 0.1 to 5% by mass with respect to the total content of the external preparation. When the content of the gelling agent is less than 0.01% by mass, it is not preferable because a sufficient viscosity cannot be obtained as a gel composition. Moreover, when there is more content of a gelatinizer than 10 mass%, since a viscosity is too high as a gel composition and stickiness is conspicuous, it is unpreferable.

  Examples of the water used for the anti-inflammatory external preparation having a gel shape according to the present invention include purified water, sterilized purified water, and water for injection, and these can be used alone or in combination of two or more.

  In addition to the above essential components, the anti-inflammatory external preparation having a gel shape according to the present invention includes various components generally used for gel base materials such as cosmetics and pharmaceuticals, that is, fats and oils, as long as the effects of the present invention are not impaired. , Waxes, ester oils, hydrocarbon oils, silicones, surfactants, lower alcohols, humectants, water-soluble polymers, UV absorbers, sequestering agents, pH adjusters, antioxidants, antibacterial agents, etc. An anti-inflammatory agent and a fragrance can be appropriately blended.

  Examples of fats and oils include linseed oil, camellia oil, macadamia nut oil, corn oil, mink oil, coconut oil, palm oil, palm kernel oil, cocoa butter, beef tallow, sheep fat, pork fat, horse fat, hydrogenated oil and hydrogenated castor An oil etc. are mentioned, It can use individually or in combination of 2 or more types.

  Examples of the waxes include beeswax, candelilla wax, cotton wax, carnauba wax, bayberry wax, ibota wax, whale wax, montan wax, nuka wax, lanolin, reduced lanolin, hard lanolin, kapok wax, sugar cane wax, and jojoba wax. Two or more types can be used in combination.

  Examples of the ester oil include octanoic acid ester, tri-2-ethylhexaenoic acid glycerin, isooctanoic acid ester, lauric acid ester, isopropyl myristate, myristic acid ester, palmitic acid ester, stearic acid ester, isostearic acid ester, A palmitic acid ester, an oleic acid ester, a sebacic acid diester, etc. are mentioned, It can use individually or in combination of 2 or more types.

  Examples of the hydrocarbon oil include liquid paraffin, ozokerite, squalane, squalene, pristane, paraffin, isoparaffin, ceresin, petrolatum, and microcrystalline wax, and these can be used alone or in combination of two or more.

  Examples of the silicone include chain silicones such as dimethylpolysiloxane, methylphenylpolysiloxane, and methylhydrogenpolysiloxane, octamethylcyclotetrasiloxane, decamethylcyclopentasiloxane, and dodecamethylcyclohexasiloxane. Two or more types can be used in combination.

  Examples of the surfactant include sodium laurate, benzalkonium chloride, 2-undecyl-N, N, N- (hydroxyethylcarboxymethyl) -2-imidazoline sodium, sorbitan monooleate, and the like. Two or more types can be used in combination.

  Examples of the lower alcohol include methanol, ethanol, propanol, isopropanol, and butanol, and these can be used alone or in combination of two or more.

  Examples of the humectant include sorbitol, polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin and diglycerin, and these can be used alone or in combination of two or more.

  Examples of water-soluble polymers include gum arabic, gum tragacanth, galactan, carob gum, guar gum, xanthan gum, caraya gum, carrageenan, pectin, agar, collagen, casein, albumin, gelatin, methylcellulose, nitrocellulose, ethylcellulose, methylhydroxypropylcellulose, Examples include sodium cellulose sulfate, hydroxypropyl cellulose, sodium carboxymethyl cellulose, crystalline cellulose, sodium alginate, propylene glycol alginate, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyethyl acrylate, polyacrylamide, bentonite, and magnesium aluminum silicate. , Single or two or more It can be used in conjunction seen.

  Examples of the UV absorber include benzoic acid UV absorbers such as paraaminobenzoic acid, methyl anthranilate, octyl salicylate, phenyl salicylate, homomethyl salicylate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, paramethoxysilicate. Examples include 2-ethylhexyl cinnamate, glyceryl mono-2-ethylhexanoate diparamethoxycinnamate, 2,4-dihydroxybenzophenone, and 2-hydroxy-4-methoxybenzophenone, which should be used alone or in combination of two or more. Can do.

  Examples of the sequestering agent include sodium edetate, sodium polyphosphate, sodium metaphosphate, phosphoric acid, and the like, and these can be used alone or in combination of two or more.

  Examples of the pH adjuster include lactic acid, citric acid, glycolic acid, succinic acid, tartaric acid, dl-malic acid, potassium carbonate, sodium hydrogen carbonate and ammonium hydrogen carbonate. These may be used alone or in combination of two or more. Can do.

  Examples of the antioxidant include ascorbic acid, α-tocopherol, dibutylhydroxytoluene and butylhydroxyanisole, and can be used alone or in combination of two or more.

  Antibacterial agents include, for example, benzoic acid, salicylic acid, carboxylic acid, sorbic acid, paraoxybenzoic acid ester, parachloromethcresol, hexachlorophene, benzalkonium chloride, chlorhexidine chloride, trichlorocarbanilide, phenoxyethanol, and the like. Or it can use in combination of 2 or more types.

  Other anti-inflammatory agents include, for example, menthol, camphor, zinc oxide, mefenamic acid and its derivatives, phenylbutazone and its derivatives, indomethacin and its derivatives, ibuprofen and its derivatives, ketoprofen and its derivatives, diclofenac sodium, diphenhydramine, tranexam Plants such as acid and derivatives thereof, dexamethasone, cortisone and esters thereof, hydrocortisone and esters thereof, corticosteroids such as prednisone and prednisolone, antihistamines, ages, oats, licorice, peony, assembly, mint, loquat, lavender and rosemary The component derived from a plant etc. are mentioned, It can use individually or in combination of 2 or more types.

  Examples of the fragrance include hydrocarbon-based fragrances such as pinene and limonene, alcohol-based fragrances such as linalool, geraniol, citronellol, menthol, borneol, benzyl alcohol, anis alcohol and β-phenethyl alcohol, and phenol-based fragrances such as anethole and eugenol. , N-butyraldehyde, isobutyraldehyde, hexyl aldehyde, citral, citronellal, benzaldehyde, aldehyde fragrance such as cinnamaldehyde, ketone fragrance such as carvone, menthone, camphor, acetophenone, ionone, γ-butyl lactone, coumarin, cineol Lactone fragrances such as octyl acetate, benzyl acetate, cinnamyl acetate, butyl propionate, methyl benzoate and other ester fragrances These can be used alone or in combination of two or more.

  When the anti-inflammatory external preparation of the present invention is in a stick shape, an oily extract fraction or an aqueous extract fraction, an alkali metal salt of a higher fatty acid, water, a monohydric alcohol and a polyhydric alcohol are essential components.

  Examples of the alkali metal salts of higher fatty acids used in the stick-like anti-inflammatory external preparation include sodium stearate, sodium palmitate, sodium myristate, sodium behenate, potassium stearate, potassium palmitate, potassium myristate and behenic acid. Potassium etc. are mentioned, It can use individually or in combination of 2 or more types. The content of the alkali metal salt of the higher fatty acid used in the anti-inflammatory external preparation having the stick shape of the present invention is preferably in the range of 3 to 15% by mass, more preferably 5% of the total content of the external preparation. -10 mass%. When the content of the alkali metal salt of the higher fatty acid is less than 3% by mass, the gel is formed and the stick shape cannot be maintained, which is not preferable. On the other hand, when the content of the alkali metal salt of higher fatty acid is more than 15% by mass, it becomes brittle and cannot withstand coating, which is not preferable.

  Examples of the water used in the anti-inflammatory external preparation having the stick shape of the present invention include purified water, sterilized purified water, and water for injection, and can be used alone or in combination of two or more.

  Examples of the monohydric alcohol used in the anti-inflammatory external preparation having a stick shape according to the present invention include methanol, ethanol, isopropanol, propanol, and butanol, and these can be used alone or in combination of two or more. The content of the monohydric alcohol used for the anti-inflammatory external preparation whose shape of the present invention is stick is preferably in the range of 10 to 50% by mass, more preferably 15 to 30% by mass of the total content of the external preparation. %. If the monohydric alcohol content is less than 10% by mass, the alkali metal salt of the higher fatty acid is not sufficiently dissolved, which is not preferable. Moreover, when there is more content of monohydric alcohol than 50 mass%, since the odor peculiar to monohydric alcohol becomes strong and motivation addition effect with respect to the exercise | movement based on an olfactory stimulus is disturbed, it is not preferable.

  Examples of the polyhydric alcohol used in the anti-inflammatory external preparation having a stick shape according to the present invention include glycerin, ethylene glycol, propylene glycol, 1,3-butanediol, polyethylene glycols, diethylene glycol and sorbitol. These can be used alone or in combination of two or more. The content of the polyhydric alcohol used in the anti-inflammatory external preparation whose shape of the present invention is a stick is preferably in the range of 15 to 50 mass%, more preferably 20 to 40 mass% of the total content of the external preparation. %. If the polyhydric alcohol content is less than 15% by mass, the alkali metal salt of the higher fatty acid is not sufficiently dissolved, which is not preferable. On the other hand, when the content of the polyhydric alcohol is more than 50% by mass, the stickiness becomes strong, which is not preferable.

  The anti-inflammatory external preparation of the present invention in the form of a stick is a variety of components generally used in stick bases such as cosmetics and pharmaceuticals, that is, fats, waxes, ester oils, hydrocarbon oils generally used in the gel base. , Silicones, surfactants, lower alcohols, moisturizers, water-soluble polymers, UV absorbers, sequestering agents, pH adjusters, antioxidants, antibacterial agents, other anti-inflammatory agents and perfumes, An alkali metal salt of an acid and an alkali metal salt of an inorganic acid can be appropriately blended.

  Examples of the alkali metal salt of the lower carboxylic acid include sodium acetate, sodium propionate, sodium lactate, sodium malate and sodium tartrate, and these can be used alone or in combination of two or more.

  Examples of the alkali metal salt of an inorganic acid include sodium bicarbonate, sodium carbonate, sodium chloride, sodium monohydrogen phosphate, sodium dihydrogen phosphate, and sodium sulfate. These can be used alone or in combination of two or more. it can.

  In the production of an anti-inflammatory external preparation whose shape of the present invention is a gel or a stick, a method conventionally known as a method for producing the below-mentioned Examples or a gel or a stick, or a method to be newly provided in the future Can be used to produce an anti-inflammatory external preparation whose target shape in the present invention is gel or stick.

The anti-inflammatory external preparation whose shape of the present invention is gel can be produced, for example, by the following method.
It can be produced by adding a gelling agent and an oily extraction fraction or an aqueous extraction fraction to purified water and stirring with a stirrer or the like until uniform.

The anti-inflammatory external preparation whose shape of the present invention is sticky can be produced, for example, by the following method.
In a suitable container, alkali metal salt of higher fatty acid, purified water, monohydric alcohol, polyhydric alcohol, alkali metal salt of lower carboxylic acid or alkali metal salt of inorganic acid, and branch and oily extract of Pinus fir plant The mixture is stirred at 70 to 90 ° C. until uniform, and the resulting solution is placed in a suitable stick container and allowed to cool and harden.

Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to the examples.
(Production Example 1)
Method for producing oily extract and aqueous extract of Todomatsu About 50 kg of Todomatsu leaves pulverized by a crushing crusher (manufactured by KYB Mfg. Co., Ltd.) is placed in the distillation tank of the microwave distillation apparatus shown in FIG. While maintaining the pressure in the distillation tank under a reduced pressure of about 20 kPa (steam temperature was about 67 ° C.), microwave irradiation was performed for 1 hour for distillation. About 180 g (about 180 mL) of the oily extract fraction and about 5 kg (about 5 L) of the aqueous extract fraction were collected from the obtained distillate.

Example 1
Based on the formulation shown in Table 1, it was prepared by the method of Preparation Method 1 described later to obtain an anti-inflammatory external preparation 1 in which the shape of the present invention is a gel. When the obtained anti-inflammatory external preparation 1 having a gel shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 80%, and there was a motivation addition effect on exercise. In addition, when the rat bruise edema suppression test was performed according to Test Example 2, the edema suppression rate was 15.9%, which showed an anti-inflammatory effect. The results are shown in Table 2.

(Preparation method 1)
0.4 g of carboxyvinyl polymer (1) was added to 19.4 g of purified water (2) and stirred to swell. To this, 0.2 g of oily extract (3) was added and stirred until it became more uniform to obtain an anti-inflammatory external preparation having a gel-like shape.

(Test Example 1)
Motivation addition confirmation test A series of actions in which 5 subjects were given 1 g of each topical preparation on the Achilles tendon and the gastrocnemius muscle (calf) for 2 minutes, and then walked at a speed of 60 to 70 m / min for 30 minutes. For 3 consecutive days. After setting a rest day from the last implementation day, the percentage of subjects who answered that they wished to walk again for 2 minutes after scenting the subject with the same topical scent was used as the motivation rate (%).

(Test Example 2)
Rat bruise edema inhibition test The normal right hind limb volume of 5 rats (Wistar / ST, 5 weeks old, male, Nippon SLC Co., Ltd.) was measured with a foot volume measuring device (TK-101 CMP, Muromachi Kikai Co., Ltd.). .
On the parafilm (Pechiny Plastic Packaging) in which 0.1 mL of each test external preparation was dropped, the rat right hind limb was moved back and forth 5 times, and each test external preparation was applied to the rat right hind limb. After the rubbing, the rat right hind limb was covered with parafilm to which each external preparation for test was adhered, and the rat was allowed to stand in a fixing holder. After standing for 3 hours, the parafilm was removed, and each external preparation for test adhered to the surface of the right hind limb was wiped off. A control group was composed of 5 rats obtained by repeating exactly the same operation except that the external preparation was not added on the parafilm.
100 g of the weight for the upper plate balance was dropped from the height of 1 m onto the rat right hind limb, and edema was induced. Four hours after edema induction, the rat right hind limb volume was measured again, and the edema rate was determined from Equation 1.

[Equation 1]
Edema rate (%) = (limb volume after edema induction / limb volume before edema induction−1) × 100.
In addition, the edema suppression rate was determined from Equation 2.

[Equation 2]
Edema suppression rate (%) = (1-average edema rate of each topical preparation / control group average edema rate) × 100

(Example 2)
In Example 1, the exact same preparation method as in Example 1 was repeated except that the content of the oily extract was increased from 0.2 g to 0.6 g, and the amount of purified water was reduced from 19.4 g to 19.0 g. Thus, an anti-inflammatory external preparation 2 having a gel shape according to the present invention was obtained. When the obtained anti-inflammatory external preparation 2 having a gel shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 100%, and there was a motivation addition effect on exercise. Moreover, when the rat bruise edema suppression test was carried out according to Test Example 2, the edema suppression rate was 26.7%, which had an anti-inflammatory effect. The results are shown in Table 2.

(Example 3)
In Example 1, the exact same preparation method as in Example 1 was repeated except that the content of the oily extract was increased from 0.2 g to 2.0 g, and the amount of purified water was reduced from 19.4 g to 17.6 g. Thus, an anti-inflammatory external preparation 3 having a gel shape of the present invention was obtained. When the obtained anti-inflammatory external preparation 3 having a gel shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 100%, and there was a motivation addition effect on exercise. Moreover, when the rat bruise edema suppression test was carried out according to Test Example 2, the edema suppression rate was 15.8%, which had an anti-inflammatory effect. The results are shown in Table 2.

Example 4
Exactly the same preparation as in Example 1 except that the oily extract fraction was changed to an aqueous extract fraction to give a content of 3.96 g, and the amount of purified water was reduced from 19.4 g to 15.64 g. By repeating the method, an anti-inflammatory external preparation 4 having a gel shape of the present invention was obtained. When the obtained anti-inflammatory external preparation 4 having a gel shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 100%, and there was a motivation addition effect on exercise. Moreover, when the rat bruise edema suppression test was carried out according to Test Example 2, the edema suppression rate was 19.8%, which had an anti-inflammatory effect. The results are shown in Table 2.

(Example 5)
In Example 4, the same preparation method as in Example 4 was repeated except that the aqueous extract fraction was increased from 3.96 g to 9.8 g, and the amount of purified water was reduced by 15.64 g to 9.8 g. The anti-inflammatory external preparation 5 whose shape of this invention is a gel form was obtained. When the obtained anti-inflammatory external preparation 5 having a gel shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 100%, and there was a motivation addition effect on exercise. Moreover, the edema suppression rate when the rat bruise edema suppression test was carried out according to Test Example 2 was 33.6%, which had an anti-inflammatory effect. The results are shown in Table 2.

(Example 6)
In Example 4, the same preparation method as in Example 4 was repeated except that the aqueous extract fraction was increased from 3.96 g to 19.6 g, and purified water was removed by that amount. An anti-inflammatory external preparation 6 was obtained. When the obtained anti-inflammatory external preparation 6 having a gel shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 100%, and there was a motivation addition effect on exercise. Moreover, when the rat bruise edema suppression test was carried out according to Test Example 2, the edema suppression rate was 18.1%, and there was an anti-inflammatory effect. The results are shown in Table 2.

(Comparative Example 1)
In Example 1, the same preparation method as in Example 1 was repeated except that 0.2 g of the oily extract fraction was not blended and the amount of purified water was increased from 19.4 g to 19.6 g. An external preparation 1 was obtained. When the obtained external preparation 1 having a gel shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 20%, and there was no motivation addition effect on exercise. Moreover, the edema suppression rate when the rat bruise edema suppression test was performed according to Test Example 2 was 6.7%, and there was no anti-inflammatory effect. The results are shown in Table 2.

(Comparative Example 2)
In Example 1, the same preparation method as in Example 1 was repeated except that the oily extract fraction content was reduced from 0.2 g to 0.06 g, and the amount of purified water was increased from 19.4 g to 19.54 g. Thus, an external preparation 2 having a gel shape was obtained. When the obtained external preparation 2 having a gel shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 20%, and there was no motivation addition effect on exercise. Moreover, the edema suppression rate when the rat bruise edema suppression test was performed according to Test Example 2 was 14.9%, and there was no anti-inflammatory effect. The results are shown in Table 2.

(Example 7)
Based on the formulation shown in Table 3, it was prepared by the method of Preparation Method 2, which will be described later, to obtain an anti-inflammatory external preparation 7 in which the shape of the present invention is a stick. When the obtained anti-inflammatory external preparation 7 having a stick shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 80%, and there was a motivation addition effect on exercise. Moreover, the edema suppression rate when the rat bruise edema suppression test was carried out according to Test Example 2 was 19.2%, which had an anti-inflammatory effect. The results are shown in Table 2.

(Preparation method 2)
Five components from sodium stearate (1) to purified water (5) were mixed and heated to reflux for 30 minutes with stirring at 70 to 90 ° C. Furthermore, 0.3 g of oily extract fraction (6) was added and stirred, and the obtained uniform solution was put into a stick container and hardened at room temperature to obtain an anti-inflammatory external preparation having a stick-like shape.

(Example 8)
In Example 7, the same preparation method as in Example 7 was repeated except that the content of the oily extract fraction was increased from 0.3 g to 1.0 g, and the amount of purified water was reduced from 42.0 g to 41.3 g. Thus, an anti-inflammatory external preparation 8 having a stick shape according to the present invention was obtained. When the obtained anti-inflammatory external preparation 8 having a stick shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 100%, and there was a motivation addition effect on exercise. Moreover, the edema suppression rate when the rat bruise edema suppression test was carried out according to Test Example 2 was 19.5%, which had an anti-inflammatory effect. The results are shown in Table 2.

Example 9
In Example 7, the same preparation method as in Example 7 was repeated, except that the oily extract fraction content was increased from 0.3 g to 3.0 g, and the amount of purified water was reduced from 42.0 g to 39.3 g. Thus, an anti-inflammatory external preparation 9 having a stick shape according to the present invention was obtained. When the obtained anti-inflammatory external preparation 9 having a stick shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 100%, and there was a motivation addition effect on exercise. Moreover, the edema suppression rate when the rat bruise edema suppression test was carried out according to Test Example 2 was 24.5%, which had an anti-inflammatory effect. The results are shown in Table 2.

(Example 10)
In Example 7, the exact same preparation method as in Example 7 was repeated, except that the oily extract fraction content was increased from 0.3 g to 10.0 g, and the amount of purified water was reduced from 42.0 g to 32.3 g. Thus, an anti-inflammatory external preparation 10 having a stick shape according to the present invention was obtained. When the obtained anti-inflammatory external preparation 10 having a stick shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 100%, and there was a motivation addition effect on exercise. Moreover, when the rat bruise edema suppression test was carried out according to Test Example 2, the edema suppression rate was 32.5%, which had an anti-inflammatory effect. The results are shown in Table 2.

(Comparative Example 3)
In Example 7, the same preparation method as in Example 7 was repeated except that 0.3 g of the oily extract fraction was not added and the amount of purified water was increased from 42.0 g to 42.3 g. Obtained an external preparation 3 having a stick-like shape. When the obtained external preparation 3 having a stick shape was subjected to a motivation addition confirmation test according to Test Example 1, the motivation addition rate was 0%, and there was no motivation addition effect on exercise. Moreover, the edema suppression rate when the rat bruise edema suppression test was carried out according to Test Example 2 was 10.3%, and there was no anti-inflammatory effect. The results are shown in Table 2.

  The present invention relates to an anti-inflammatory external preparation capable of adding motivation to exercise to help continuous exercise throughout life, and capable of preventing inflammation that may be caused by exercise. It can be used sufficiently.

DESCRIPTION OF SYMBOLS 1 ...... Microwave distillation apparatus 2 ... Distillation tank 3 ...... Microwave heating apparatus 4 ...... Stirring splash 5 ...... Airflow inflow pipe 6 ...... Distillate outflow pipe 7 ...... Cooling apparatus 8 ...... Heating control apparatus 9 ...... Pressure reducing pump 10 ... ... Pressure regulating valve 11 ... ... Pressure control device 12 ... ... Distillation target 13 ... ... Distilled product 13a ... ... Oily extract fraction 13b ... ... Aqueous extract fraction

Claims (6)

  An anti-inflammatory external preparation characterized by containing an oily extract or an aqueous extract extracted from branches and / or leaves of a plant of the genus Firaceae as an active ingredient that exhibits an anti-inflammatory effect.   The anti-inflammatory external preparation according to claim 1, wherein the plant of the genus Firaceae is Todomatsu.   The anti-inflammatory external preparation according to claim 1 or 2, wherein the content of the oily extract fraction is 0.1 to 10% by mass in the base material.   The anti-inflammatory external preparation according to claim 1 or 2, wherein the content of the aqueous extract fraction is 25 to 99.9% by mass in the base material.   The anti-inflammatory external preparation according to any one of claims 1 to 4, wherein the shape is a gel or a stick. The anti-inflammatory external preparation according to any one of claims 1 to 5, which adds motivation to exercise.

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