JP2019068823A - 組換えクロストリジウムボツリヌス神経毒 - Google Patents

組換えクロストリジウムボツリヌス神経毒 Download PDF

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Publication number
JP2019068823A
JP2019068823A JP2018230980A JP2018230980A JP2019068823A JP 2019068823 A JP2019068823 A JP 2019068823A JP 2018230980 A JP2018230980 A JP 2018230980A JP 2018230980 A JP2018230980 A JP 2018230980A JP 2019068823 A JP2019068823 A JP 2019068823A
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JP
Japan
Prior art keywords
bont
protein
nucleic acid
sequence
trypsin
Prior art date
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Ceased
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JP2018230980A
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English (en)
Japanese (ja)
Inventor
コッシンズ，エイミー
Cossins Aimee
ビアード，マシュー
Beard Matthew
マークス，フィリップ
Philip Marks
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ipsen Biopharm Ltd
Ipsen Bioinnovation Ltd
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Ipsen Biopharm Ltd
Ipsen Bioinnovation Ltd
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Application filed by Ipsen Biopharm Ltd, Ipsen Bioinnovation Ltd filed Critical Ipsen Biopharm Ltd
Publication of JP2019068823A publication Critical patent/JP2019068823A/ja
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/164—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • A61K38/4893—Botulinum neurotoxin (3.4.24.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2018230980A 2012-10-31 2018-12-10 組換えクロストリジウムボツリヌス神経毒 Ceased JP2019068823A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1219602.8 2012-10-31
GB201219602A GB201219602D0 (en) 2012-10-31 2012-10-31 Recombinant clostridium botulinum neurotoxins

Related Parent Applications (1)

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JP2015540207A Division JP2015534814A (ja) 2012-10-31 2013-10-31 組換えクロストリジウムボツリヌス神経毒

Related Child Applications (1)

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JP2020017612A Division JP2020078321A (ja) 2012-10-31 2020-02-05 組換えクロストリジウムボツリヌス神経毒

Publications (1)

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JP2019068823A true JP2019068823A (ja) 2019-05-09

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JP2018230980A Ceased JP2019068823A (ja) 2012-10-31 2018-12-10 組換えクロストリジウムボツリヌス神経毒
JP2020017612A Pending JP2020078321A (ja) 2012-10-31 2020-02-05 組換えクロストリジウムボツリヌス神経毒

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JP2020017612A Pending JP2020078321A (ja) 2012-10-31 2020-02-05 組換えクロストリジウムボツリヌス神経毒

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Country Link
US (1) US10030238B2 (enExample)
EP (3) EP3326644B1 (enExample)
JP (2) JP2019068823A (enExample)
KR (2) KR102264478B1 (enExample)
CN (2) CN104755098A (enExample)
AR (2) AR093309A1 (enExample)
AU (3) AU2013340610B2 (enExample)
BR (1) BR112015005384A2 (enExample)
CA (1) CA2885519A1 (enExample)
DK (2) DK2914282T3 (enExample)
EA (1) EA201590794A8 (enExample)
ES (2) ES2662402T3 (enExample)
GB (1) GB201219602D0 (enExample)
HU (2) HUE048802T2 (enExample)
IL (1) IL237623B (enExample)
IN (1) IN2015MN00436A (enExample)
MX (2) MX367080B (enExample)
NO (1) NO2914282T3 (enExample)
NZ (1) NZ705575A (enExample)
PL (2) PL2914282T3 (enExample)
PT (2) PT3326644T (enExample)
SG (2) SG11201502372SA (enExample)
TW (3) TWI673361B (enExample)
UA (1) UA118837C2 (enExample)
WO (1) WO2014068317A1 (enExample)
ZA (1) ZA201501449B (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023545000A (ja) * 2020-10-01 2023-10-26 イプセン バイオファーム リミテッド ベータトリプシンを生成する方法

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GB201219602D0 (en) * 2012-10-31 2012-12-12 Syntaxin Ltd Recombinant clostridium botulinum neurotoxins
RU2746736C2 (ru) 2015-03-26 2021-04-20 Президент Энд Феллоуз Оф Гарвард Колледж Сконструированный ботулинический нейротоксин
WO2017063743A1 (en) 2015-10-14 2017-04-20 Merz Pharma Gmbh & Co. Kgaa Improvements to ultrasound-based therapy of photoaged tissue
MX395130B (es) 2016-03-02 2025-03-24 Merz Pharma Gmbh & Co Kgaa Composicion que comprende toxina botulinica
EP3263710A1 (en) * 2016-07-01 2018-01-03 Ipsen Biopharm Limited Production of activated clostridial neurotoxins
EP3504226A1 (en) 2016-08-24 2019-07-03 President and Fellows of Harvard College Engineered botulinum neurotoxin
JP7217700B2 (ja) 2016-09-13 2023-02-03 アラーガン、インコーポレイテッド 安定化非タンパク質クロストリジウム毒素組成物
EP3642222A1 (en) 2017-06-20 2020-04-29 Merz Pharma GmbH & Co. KGaA Novel recombinant botulinum toxin with increased duration of effect
US11155802B2 (en) 2017-07-06 2021-10-26 Merz Pharma Gmbh & Co. Kgaa Recombinant botulinum neurotoxins with increased duration of effect
CA3078631A1 (en) 2017-10-11 2019-04-18 Jacob M. Waugh Methods and compositions for topical delivery
BR112020006827A2 (pt) * 2018-01-29 2020-10-06 Ipsen Biopharm Limited neurotoxinas botulínicas clivando snare não neuronal
US11707510B2 (en) * 2018-02-16 2023-07-25 Preclinics Discovery Gmbh Nucleic acid-based botulinum neurotoxin for therapeutic use
GB201815844D0 (en) * 2018-09-28 2018-11-14 Ipsen Biopharm Ltd Therapeutic & comestic uses of botulinum neurotoxin serotype e
GB202011055D0 (en) 2020-07-17 2020-09-02 Ipsen Bioinnovation Ltd Treatment of post-operative pain
CN115819526A (zh) * 2022-12-02 2023-03-21 海雅美生物技术(珠海)有限公司 一种重组肉毒杆菌神经毒素及其制备方法和应用

Citations (2)

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WO2006011966A1 (en) * 2004-06-30 2006-02-02 Allergan, Inc. Optimizing expression of active botulinum toxin type e
JP2012518403A (ja) * 2009-02-23 2012-08-16 シンタクシン リミテッド 改変非細胞毒性プロテアーゼ

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NZ308772A (en) * 1995-05-17 1999-04-29 Du Pont Recombinant baculovirus insecticides
WO2000002524A2 (en) 1998-07-10 2000-01-20 U.S. Army Medical Research Institute Of Infectious Diseases Botulinum neurotoxin vaccine
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US7374896B2 (en) 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
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EP3504226A1 (en) * 2016-08-24 2019-07-03 President and Fellows of Harvard College Engineered botulinum neurotoxin

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2006011966A1 (en) * 2004-06-30 2006-02-02 Allergan, Inc. Optimizing expression of active botulinum toxin type e
JP2012518403A (ja) * 2009-02-23 2012-08-16 シンタクシン リミテッド 改変非細胞毒性プロテアーゼ

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023545000A (ja) * 2020-10-01 2023-10-26 イプセン バイオファーム リミテッド ベータトリプシンを生成する方法
JP7794816B2 (ja) 2020-10-01 2026-01-06 イプセン バイオファーム リミテッド ベータトリプシンを生成する方法

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Publication number Publication date
GB201219602D0 (en) 2012-12-12
AU2013340610B2 (en) 2018-02-22
TWI626307B (zh) 2018-06-11
PL3326644T3 (pl) 2020-08-24
AU2018203556B2 (en) 2020-08-20
TWI673361B (zh) 2019-10-01
DK2914282T3 (en) 2018-02-26
UA118837C2 (uk) 2019-03-25
KR20150094590A (ko) 2015-08-19
HUE048802T2 (hu) 2020-08-28
AU2020244481A1 (en) 2020-10-29
PT3326644T (pt) 2020-05-29
EP3326644A1 (en) 2018-05-30
KR102264478B1 (ko) 2021-06-15
HK1208359A1 (en) 2016-03-04
PT2914282T (pt) 2018-03-13
CN104755098A (zh) 2015-07-01
EP3673914B1 (en) 2021-10-27
SG11201502372SA (en) 2015-05-28
NO2914282T3 (enExample) 2018-05-19
KR102188539B1 (ko) 2020-12-09
SG10201701140WA (en) 2017-04-27
MX367080B (es) 2019-08-05
IL237623A0 (en) 2015-04-30
WO2014068317A1 (en) 2014-05-08
MX2019009222A (es) 2019-11-21
TW201435084A (zh) 2014-09-16
EA201590794A8 (ru) 2016-01-29
US10030238B2 (en) 2018-07-24
TW202012622A (zh) 2020-04-01
CA2885519A1 (en) 2014-05-08
KR20200110470A (ko) 2020-09-23
ES2788199T3 (es) 2020-10-20
TW201825680A (zh) 2018-07-16
ES2662402T3 (es) 2018-04-06
HUE036764T2 (hu) 2018-07-30
PL2914282T3 (pl) 2018-07-31
AR117738A2 (es) 2021-08-25
EP2914282A1 (en) 2015-09-09
JP2020078321A (ja) 2020-05-28
IN2015MN00436A (enExample) 2015-09-04
BR112015005384A2 (pt) 2017-08-08
EP3673914A1 (en) 2020-07-01
NZ705575A (en) 2019-08-30
US20150247139A1 (en) 2015-09-03
AU2018203556A1 (en) 2018-06-07
AR093309A1 (es) 2015-05-27
EA201590794A1 (ru) 2015-08-31
EP2914282B1 (en) 2017-12-20
DK3326644T3 (da) 2020-05-11
ZA201501449B (en) 2016-08-31
IL237623B (en) 2019-05-30
EP3326644B1 (en) 2020-03-04
CN110499321A (zh) 2019-11-26
AU2013340610A1 (en) 2015-03-19
MX2015005156A (es) 2015-09-23

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