JP2018518967A5 - - Google Patents
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- JP2018518967A5 JP2018518967A5 JP2017566374A JP2017566374A JP2018518967A5 JP 2018518967 A5 JP2018518967 A5 JP 2018518967A5 JP 2017566374 A JP2017566374 A JP 2017566374A JP 2017566374 A JP2017566374 A JP 2017566374A JP 2018518967 A5 JP2018518967 A5 JP 2018518967A5
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- JP
- Japan
- Prior art keywords
- nucleic acid
- target
- complementary
- probe
- methylated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562183854P | 2015-06-24 | 2015-06-24 | |
| US62/183,854 | 2015-06-24 | ||
| PCT/US2016/039167 WO2016210224A1 (en) | 2015-06-24 | 2016-06-24 | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018518967A JP2018518967A (ja) | 2018-07-19 |
| JP2018518967A5 true JP2018518967A5 (https=) | 2019-07-25 |
| JP6941568B2 JP6941568B2 (ja) | 2021-09-29 |
Family
ID=57585693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017566374A Active JP6941568B2 (ja) | 2015-06-24 | 2016-06-24 | ヌクレアーゼを使用する野生型ｄｎａの選択的分解および突然変異体対立遺伝子の濃縮 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11725230B2 (https=) |
| EP (1) | EP3313992B1 (https=) |
| JP (1) | JP6941568B2 (https=) |
| AU (1) | AU2016281718B2 (https=) |
| CA (1) | CA2990846C (https=) |
| ES (1) | ES2917175T3 (https=) |
| WO (1) | WO2016210224A1 (https=) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2990846C (en) | 2015-06-24 | 2023-09-26 | Dana-Farber Cancer Institute, Inc. | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| WO2019023243A1 (en) | 2017-07-24 | 2019-01-31 | Dana-Farber Cancer Institute, Inc. | METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE |
| CN107893109B (zh) * | 2017-11-08 | 2021-07-06 | 重庆邮电大学 | 一种基于移除野生型序列的低丰度基因突变富集方法 |
| KR102086689B1 (ko) * | 2018-02-06 | 2020-03-09 | 주식회사 진씨커 | 돌연변이 세포 유리 유전자 분리 키트 및 이를 이용한 돌연변이 세포 유리 유전자 분리 방법 |
| CN119753091A (zh) | 2018-04-02 | 2025-04-04 | 格里尔公司 | 用于扩增与癌症相关联的cfDNA分子的化验板 |
| CN108531603A (zh) * | 2018-05-31 | 2018-09-14 | 青岛普泽麦迪生物技术有限公司 | 一种用于braf基因v600e突变检测的引物对、试剂盒及其检测方法 |
| IL281741B2 (en) | 2018-09-27 | 2026-04-01 | Grail Inc | Methylation markers and a targeted methylation detection panel |
| WO2020081629A1 (en) * | 2018-10-16 | 2020-04-23 | University Of Cincinnati | Single-molecule epigenetic localization |
| CN109880891B (zh) * | 2019-04-22 | 2021-07-30 | 上海交通大学 | 基于核酸酶偶联pcr原理富集低丰度dna突变的检测技术体系及应用 |
| EP4017998B1 (en) | 2019-08-21 | 2024-11-06 | Dana-Farber Cancer Institute, Inc. | Multi-site enrichment of deletions in dna microsatellites |
| WO2022032195A2 (en) | 2020-08-06 | 2022-02-10 | Singular Genomics Systems, Inc. | Spatial sequencing |
| EP4121561A4 (en) | 2020-08-06 | 2024-04-17 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| GB2603203A (en) * | 2021-02-02 | 2022-08-03 | Univ Heriot Watt | Method for enriching nucelic acids |
| WO2022239011A1 (en) * | 2021-05-14 | 2022-11-17 | Carmel - Haifa University Economic Corporation Ltd | A method of identifying ultra-rare genetic variants |
| US11993792B2 (en) | 2021-05-27 | 2024-05-28 | New England Biolabs, Inc. | DNase I variants, compositions, methods, and kits |
| GB202111195D0 (en) * | 2021-08-03 | 2021-09-15 | Cergentis B V | Method for targeted sequencing |
| CN114280128B (zh) * | 2021-12-24 | 2022-11-18 | 清华大学 | 双标记gFET的制备及其在miRNA检测中的应用 |
| CN120366267A (zh) * | 2022-07-01 | 2025-07-25 | 中国科学院基础医学与肿瘤研究所(筹) | 一种基于可编程核酸酶的超灵敏目标核酸富集检测方法 |
| JP2025536281A (ja) * | 2022-10-19 | 2025-11-05 | バイオフィデリティ・リミテッド | 核酸の濃縮および検出 |
| EP4720332A1 (en) | 2023-06-01 | 2026-04-08 | Singular Genomics Systems, Inc. | Methods and probes for detecting polynucleotide sequences in cells and tissues |
| WO2025029475A1 (en) * | 2023-07-28 | 2025-02-06 | Guardant Health, Inc. | Methods to enrich nucleotide variants by negative selection |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5670316A (en) | 1990-05-07 | 1997-09-23 | Daikin Industries, Ltd. | Diagnostic applications of double D-loop formation |
| AU4681693A (en) * | 1992-07-17 | 1994-02-14 | Aprogenex, Inc. | Nucleic acid probes and uses thereof to detect double-stranded nucleic acids |
| US5629178A (en) | 1994-10-28 | 1997-05-13 | Genetics & Ivf Institute | Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA) |
| US6949340B2 (en) * | 2001-03-28 | 2005-09-27 | Creative Mines Llc | Optical phase modulator |
| DE10158516A1 (de) | 2001-11-29 | 2003-06-12 | Focusgenomics Gmbh | Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren |
| AU2002346662A1 (en) * | 2001-12-04 | 2003-06-17 | Euregen Llc | Methods and compositions for selectively cleaving dna containing duplex acids in a complex nucleic acid mixture |
| EP2290106B1 (en) * | 2004-03-08 | 2018-01-03 | Rubicon Genomics, Inc. | Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation |
| US7749707B2 (en) | 2005-03-01 | 2010-07-06 | Wako Pure Chemical Industries, Ltd. | Method for obtaining subtraction polynucleotide |
| US20090099075A1 (en) * | 2005-11-24 | 2009-04-16 | Basf Se | Chimeric Keratin-Binding Effector Proteins |
| EP1987162A4 (en) | 2006-01-23 | 2009-11-25 | Population Genetics Technologi | NUCLEIC ACID ANALYSIS ABOUT SEQUENCE TOKENS |
| WO2008096146A1 (en) | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
| US20080254453A1 (en) | 2007-04-12 | 2008-10-16 | Affymetrix, Inc | Analysis of methylation using selective adaptor ligation |
| US7749708B2 (en) * | 2007-09-12 | 2010-07-06 | Transgenomic, Inc. | Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule |
| US9434988B2 (en) * | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| DK2398915T3 (en) * | 2009-02-20 | 2016-12-12 | Synthetic Genomics Inc | Synthesis of nucleic acids sequence verified |
| US10636413B2 (en) | 2009-06-13 | 2020-04-28 | Rolr, Inc. | System for communication skills training using juxtaposition of recorded takes |
| EP2272976A1 (en) * | 2009-07-06 | 2011-01-12 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for differentiation of polynucleotide strands |
| US20130059734A1 (en) | 2009-11-13 | 2013-03-07 | Commonwealth Scientific And Industrial Research Organisation | Epigenetic analysis |
| CN102859003B (zh) | 2010-03-08 | 2014-07-30 | 达纳-法伯癌症研究院有限公司 | 使用参考封闭序列的全cold-pcr富集 |
| US9260753B2 (en) | 2011-03-24 | 2016-02-16 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| EP3333269B1 (en) | 2011-03-31 | 2021-05-05 | Dana-Farber Cancer Institute, Inc. | Methods to enable multiplex cold-pcr |
| JP6040227B2 (ja) | 2011-05-19 | 2016-12-07 | アジーナ バイオサイエンス， インコーポレイテッド | マルチプレックス核酸同定のための製品およびプロセス |
| US9238836B2 (en) * | 2012-03-30 | 2016-01-19 | Pacific Biosciences Of California, Inc. | Methods and compositions for sequencing modified nucleic acids |
| CN105189746B (zh) | 2013-03-14 | 2018-12-04 | 宝生物工程株式会社 | 用于使用耐热的错配内切核酸酶的方法 |
| BR112015026499B1 (pt) | 2013-04-17 | 2022-06-21 | Pioneer Hi-Bred International, Inc | Método para caracterizar uma sequência alvo |
| US10913977B2 (en) | 2013-07-24 | 2021-02-09 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR |
| US9873908B2 (en) | 2013-11-27 | 2018-01-23 | Roche Molecular Systems, Inc. | Methods for the enrichment of mutated nucleic acid from a mixture |
| ES2660989T3 (es) | 2013-12-28 | 2018-03-27 | Guardant Health, Inc. | Métodos y sistemas para detectar variantes genéticas |
| CA2990846C (en) | 2015-06-24 | 2023-09-26 | Dana-Farber Cancer Institute, Inc. | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| EP4017998B1 (en) | 2019-08-21 | 2024-11-06 | Dana-Farber Cancer Institute, Inc. | Multi-site enrichment of deletions in dna microsatellites |
-
2016
- 2016-06-24 CA CA2990846A patent/CA2990846C/en active Active
- 2016-06-24 WO PCT/US2016/039167 patent/WO2016210224A1/en not_active Ceased
- 2016-06-24 JP JP2017566374A patent/JP6941568B2/ja active Active
- 2016-06-24 US US15/739,301 patent/US11725230B2/en active Active
- 2016-06-24 AU AU2016281718A patent/AU2016281718B2/en active Active
- 2016-06-24 EP EP16815352.6A patent/EP3313992B1/en active Active
- 2016-06-24 ES ES16815352T patent/ES2917175T3/es active Active
-
2023
- 2023-08-13 US US18/448,941 patent/US20230392191A1/en active Pending
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