JP2018086016A5 - - Google Patents
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- JP2018086016A5 JP2018086016A5 JP2018027616A JP2018027616A JP2018086016A5 JP 2018086016 A5 JP2018086016 A5 JP 2018086016A5 JP 2018027616 A JP2018027616 A JP 2018027616A JP 2018027616 A JP2018027616 A JP 2018027616A JP 2018086016 A5 JP2018086016 A5 JP 2018086016A5
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- probe
- nucleic acid
- detection target
- signal intensity
- bacteria
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- 239000000523 sample Substances 0.000 claims 15
- 241000894006 Bacteria Species 0.000 claims 11
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 238000001514 detection method Methods 0.000 claims 6
- 230000001580 bacterial Effects 0.000 claims 4
- 241000894007 species Species 0.000 claims 4
- 210000000214 Mouth Anatomy 0.000 claims 3
- 238000009396 hybridization Methods 0.000 claims 3
- 108020004465 16S Ribosomal RNA Proteins 0.000 claims 2
- 210000000936 Intestines Anatomy 0.000 claims 2
- 210000003491 Skin Anatomy 0.000 claims 2
- 230000000295 complement Effects 0.000 claims 2
- 239000010802 sludge Substances 0.000 claims 2
- 239000002689 soil Substances 0.000 claims 2
- 238000000018 DNA microarray Methods 0.000 claims 1
- 241000605894 Porphyromonas Species 0.000 claims 1
- 241001470488 Tannerella Species 0.000 claims 1
- 241000589886 Treponema Species 0.000 claims 1
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
Claims (5)
- 以下のプローブ（ａ）とプローブ（ｃ）とを搭載した、細菌叢解析用デバイス。
（ａ）腸内、皮膚、口内、土壌、海水、河川又は活性汚泥に含まれる細菌から選ばれる検出の対象となる２種以上の細菌のそれぞれに特異的な１６ＳｒＲＮＡにハイブリダイズする核酸からなるプローブ、又は当該核酸の相補配列核酸からなるプローブ
（ｃ）被検試料中に含まれる細菌に由来する核酸にはハイブリダイズせず、被検試料中に含まれる細菌のＤＮＡが増幅されないように人工的に設計された核酸にハイブリダイズする、１種類又は複数 種類の絶対量指標プローブ - デバイスが、繊維型ＤＮＡマイクロアレイである請求項１に記載のデバイス。
- 口内に含まれる細菌が、Ｐｏｒｐｈｙｒｏｍｏｎａｓ属、Ｔａｎｎｅｒｅｌｌａ属又はＴｒｅｐｏｎｅｍａ属に属する細菌である、請求項１又は２に記載のデバイス。
- 以下の工程を含む、検出対象菌種のコピー数を演算する方法。
（１）予め単離された検出対象細菌のそれぞれについて、腸内、皮膚、口内、土壌、海水、河川又は活性汚泥に含まれる細菌から選ばれる検出の対象となる２種以上の細菌のそれぞれに特異的な１６ＳｒＲＮＡにハイブリダイズする核酸からなるプローブ、又は当該核酸の相補配列核酸からなるプローブのシグナル強度を測定する工程
（２）前記シグナル強度と、絶対量指標プローブのシグナル強度とを比較し、係数を算出する工程
（３）前記（２）の工程で算出した算出値をプローブのハイブリダイゼーション効率係数とし、当該ハイブリダイゼーション効率係数を用いて被検試料から得られるデータの各検出対象菌種のコピー数を演算する工程 - 以下の工程を含む、検出対象菌種の絶対量を推定する方法。
（１）予め単離された検出対象細菌のそれぞれについて、当該検出対象細菌を検出するためのプローブのシグナル強度比から係数を算出する工程
（２）上記（１）の工程で算出した算出値をプローブのハイブリダイゼーション効率係数とし、当該ハイブリダイゼーション効率係数を用いて被検サンプルから得られるデータの各検出対象菌種のコピー数を演算する工程
（３）上記（２）の工程で演算した演算後のシグナル強度を、絶対量指標プローブのシグナル強度と比較する工程
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014098719 | 2014-05-12 | ||
| JP2014098719 | 2014-05-12 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015089562A Division JP6299660B2 (ja) | 2014-05-12 | 2015-04-24 | 菌叢解析方法と菌叢解析用デバイス |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020129939A Division JP7040562B2 (ja) | 2014-05-12 | 2020-07-31 | 菌叢解析方法と菌叢解析用デバイス |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018086016A JP2018086016A (ja) | 2018-06-07 |
| JP2018086016A5 true JP2018086016A5 (ja) | 2018-07-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2015089562A Active JP6299660B2 (ja) | 2014-05-12 | 2015-04-24 | 菌叢解析方法と菌叢解析用デバイス |
| JP2018027616A Pending JP2018086016A (ja) | 2014-05-12 | 2018-02-20 | 菌叢解析方法と菌叢解析用デバイス |
| JP2020129939A Active JP7040562B2 (ja) | 2014-05-12 | 2020-07-31 | 菌叢解析方法と菌叢解析用デバイス |
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| JP2015089562A Active JP6299660B2 (ja) | 2014-05-12 | 2015-04-24 | 菌叢解析方法と菌叢解析用デバイス |
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| JP2020129939A Active JP7040562B2 (ja) | 2014-05-12 | 2020-07-31 | 菌叢解析方法と菌叢解析用デバイス |
Country Status (1)
| Country | Link |
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| JP (3) | JP6299660B2 (ja) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6299660B2 (ja) * | 2014-05-12 | 2018-03-28 | 三菱ケミカル株式会社 | 菌叢解析方法と菌叢解析用デバイス |
| CN109790531A (zh) * | 2016-07-11 | 2019-05-21 | 三菱化学株式会社 | 口腔内检查方法 |
| US20180148769A1 (en) * | 2016-11-25 | 2018-05-31 | Mitsubishi Chemical Corporation | Bacterial flora analysis method and bacterial flora analysis device |
| JP6959021B2 (ja) * | 2017-03-14 | 2021-11-02 | 株式会社ジーシー | 齲蝕細菌検出用ｄｎａチップ |
| KR102575756B1 (ko) * | 2018-05-10 | 2023-09-07 | 주식회사 씨젠 | 내부 대조군으로 장내 정상세균총을 이용하여 시료로부터 장내 미생물을 검출하는 방법 |
| CN112166200A (zh) * | 2018-05-25 | 2021-01-01 | 三菱化学株式会社 | 目标核酸的定量方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT528306E (pt) * | 1991-08-15 | 2000-05-31 | Hoffmann La Roche | Iniciadores e sondas de micobacterias |
| AUPQ909000A0 (en) * | 2000-07-28 | 2000-08-24 | University Of Sydney, The | A method of detecting microorganisms |
| WO2003083127A2 (en) * | 2002-03-28 | 2003-10-09 | Deutsches Krebsforschungszentrum | Method and system for determining absolute mrna quantities |
| JP2003284559A (ja) * | 2002-03-28 | 2003-10-07 | Masao Nasu | 細菌種同定方法 |
| JP2004065242A (ja) * | 2002-06-13 | 2004-03-04 | Mitsubishi Rayon Co Ltd | 生体関連物質マイクロアレイ及びそれを用いた植物の品種判別法 |
| JP2005270093A (ja) * | 2004-02-24 | 2005-10-06 | Nippon Medical School | 乳癌の術後予後予測に関与する遺伝子 |
| ES2426038T3 (es) * | 2006-02-21 | 2013-10-18 | National University Corporation University Of Toyama | Procedimiento rápido para identificar el microorganismo causante de una enfermedad infecciosa |
| CA2652319A1 (en) * | 2006-05-17 | 2007-11-22 | Eppendorf Array Technologies S.A. | Identification and quantification of a plurality of biological (micro)organisms or their components |
| JP2009072168A (ja) * | 2007-09-18 | 2009-04-09 | Univ Of Occupational & Environmental Health Japan | 微生物の判別方法 |
| EP2218780B1 (en) * | 2007-10-19 | 2013-07-24 | Eiken Kagaku Kabushiki Kaisha | Nucleic acid amplification method, and reagent and reagent kit for use in the method |
| JP2009145058A (ja) * | 2007-12-11 | 2009-07-02 | Mitsubishi Rayon Co Ltd | 核酸マイクロアレイ及びその製造方法 |
| JP6299660B2 (ja) * | 2014-05-12 | 2018-03-28 | 三菱ケミカル株式会社 | 菌叢解析方法と菌叢解析用デバイス |
-
2015
- 2015-04-24 JP JP2015089562A patent/JP6299660B2/ja active Active
-
2018
- 2018-02-20 JP JP2018027616A patent/JP2018086016A/ja active Pending
-
2020
- 2020-07-31 JP JP2020129939A patent/JP7040562B2/ja active Active
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