JP2015231362A5 - - Google Patents

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JP2015231362A5
JP2015231362A5 JP2015089562A JP2015089562A JP2015231362A5 JP 2015231362 A5 JP2015231362 A5 JP 2015231362A5 JP 2015089562 A JP2015089562 A JP 2015089562A JP 2015089562 A JP2015089562 A JP 2015089562A JP 2015231362 A5 JP2015231362 A5 JP 2015231362A5
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Japan
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probe
detection target
bacteria
calculating
following
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JP2015089562A
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JP6299660B2 (ja
JP2015231362A (ja
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Publication of JP2015231362A5 publication Critical patent/JP2015231362A5/ja
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Claims (3)

  1. 以下のプローブ（ａ）とプローブ（ｂ）とを搭載したデバイス。
    （ａ）検出の対象となる１種又は２種以上の細菌のそれぞれに特異的な１６ＳｒＲＮＡにハイブリダイズする核酸からなるプローブ
    （ｂ）前記１種又は２種以上の細菌が共通に有する塩基配列を含む総量指標プローブ
  2. さらに以下のプローブ（ｃ）を搭載した、請求項１に記載のデバイス。
    （ｃ）１種類又は複数種類の絶対量指標プローブ
  3. 以下の工程を含む、被験サンプルから得られるデータの各検出対象菌種のコピー数を演算する方法。
    （１）予め単離された検出対象細菌のそれぞれについて、当該検出対象細菌を検出するためのプローブのシグナル強度比から係数を算出する工程
    （２）上記（１）の工程で算出した算出値をプローブのハイブリダイゼーション効率係数とし、当該ハイブリダイゼーション効率係数を用いて被検サンプルから得られるデータの各検出対象菌種のコピー数を演算する工程
JP2015089562A 2014-05-12 2015-04-24 菌叢解析方法と菌叢解析用デバイス Active JP6299660B2 (ja)

Priority Applications (1)

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JP2015089562A JP6299660B2 (ja) 2014-05-12 2015-04-24 菌叢解析方法と菌叢解析用デバイス

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JP2014098719 2014-05-12
JP2014098719 2014-05-12
JP2015089562A JP6299660B2 (ja) 2014-05-12 2015-04-24 菌叢解析方法と菌叢解析用デバイス

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JP2018027616A Division JP2018086016A (ja) 2014-05-12 2018-02-20 菌叢解析方法と菌叢解析用デバイス

Publications (3)

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JP2015231362A JP2015231362A (ja) 2015-12-24
JP2015231362A5 true JP2015231362A5 (ja) 2017-08-24
JP6299660B2 JP6299660B2 (ja) 2018-03-28

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JP2018027616A Pending JP2018086016A (ja) 2014-05-12 2018-02-20 菌叢解析方法と菌叢解析用デバイス
JP2020129939A Active JP7040562B2 (ja) 2014-05-12 2020-07-31 菌叢解析方法と菌叢解析用デバイス

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Families Citing this family (6)

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Publication number Priority date Publication date Assignee Title
JP6299660B2 (ja) * 2014-05-12 2018-03-28 三菱ケミカル株式会社 菌叢解析方法と菌叢解析用デバイス
JPWO2018012011A1 (ja) * 2016-07-11 2018-07-19 三菱ケミカル株式会社 口腔内検査方法
US20180148769A1 (en) * 2016-11-25 2018-05-31 Mitsubishi Chemical Corporation Bacterial flora analysis method and bacterial flora analysis device
JP6959021B2 (ja) * 2017-03-14 2021-11-02 株式会社ジーシー 齲蝕細菌検出用ｄｎａチップ
EP3790988A4 (en) * 2018-05-10 2021-12-01 Seegene, Inc. METHOD OF DETECTING INTESTINAL MICROORGANISMS IN A SAMPLE WITH NORMAL INTESTINAL FLORA AS AN INTERNAL CONTROL
CN112166200A (zh) * 2018-05-25 2021-01-01 三菱化学株式会社 目标核酸的定量方法

Family Cites Families (12)

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ES2140400T3 (es) * 1991-08-15 2000-03-01 Hoffmann La Roche Cebadores y sondas del micobacterium.
AUPQ909000A0 (en) * 2000-07-28 2000-08-24 University Of Sydney, The A method of detecting microorganisms
US20050255466A1 (en) * 2002-03-28 2005-11-17 Deutsches Krebsforschungszntrum Method and system for determining absolute mrna quantities
JP2003284559A (ja) * 2002-03-28 2003-10-07 Masao Nasu 細菌種同定方法
JP2004065242A (ja) * 2002-06-13 2004-03-04 Mitsubishi Rayon Co Ltd 生体関連物質マイクロアレイ及びそれを用いた植物の品種判別法
JP2005270093A (ja) * 2004-02-24 2005-10-06 Nippon Medical School 乳癌の術後予後予測に関与する遺伝子
EP1997886B1 (en) * 2006-02-21 2013-06-05 National University Corporation University of Toyama Rapid method for identifying causative microorganism of infectious disease
WO2007132002A1 (en) * 2006-05-17 2007-11-22 Eppendorf Array Technologies S.A. Identification and quantification of a plurality of biological (micro)organisms or their components
JP2009072168A (ja) * 2007-09-18 2009-04-09 Univ Of Occupational & Environmental Health Japan 微生物の判別方法
ES2433667T3 (es) * 2007-10-19 2013-12-12 Eiken Kagaku Kabushiki Kaisha Método de amplificación de ácidos nucleicos, y reactivo y kit de reactivos para la utilización en el método
JP2009145058A (ja) * 2007-12-11 2009-07-02 Mitsubishi Rayon Co Ltd 核酸マイクロアレイ及びその製造方法
JP6299660B2 (ja) * 2014-05-12 2018-03-28 三菱ケミカル株式会社 菌叢解析方法と菌叢解析用デバイス

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