JP2017535405A5 - - Google Patents

Info

Publication number

JP2017535405A5

JP2017535405A5 JP2017547081A JP2017547081A JP2017535405A5 JP 2017535405 A5 JP2017535405 A5 JP 2017535405A5 JP 2017547081 A JP2017547081 A JP 2017547081A JP 2017547081 A JP2017547081 A JP 2017547081A JP 2017535405 A5 JP2017535405 A5 JP 2017535405A5

Authority

JP

Japan

Prior art keywords

chitosan

cells

chondrocytes

dimensional structure

hydrogel

Prior art date

2015-12-01

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 claims 21
• 210000004027 cell Anatomy 0.000 claims 20
• 229920001661 Chitosan Polymers 0.000 claims 19
• 239000000017 hydrogel Substances 0.000 claims 14
• 210000001612 chondrocyte Anatomy 0.000 claims 12
• 239000002245 particle Substances 0.000 claims 10
• 239000000203 mixture Substances 0.000 claims 9
• 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims 7
• 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims 7
• 210000002744 extracellular matrix Anatomy 0.000 claims 7
• KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims 5
• 230000015572 biosynthetic process Effects 0.000 claims 5
• 229920002674 hyaluronan Polymers 0.000 claims 5
• 229960003160 hyaluronic acid Drugs 0.000 claims 5
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 4
• 210000000130 stem cell Anatomy 0.000 claims 4
• 238000003786 synthesis reaction Methods 0.000 claims 4
• 229920006318 anionic polymer Polymers 0.000 claims 3
• 210000000845 cartilage Anatomy 0.000 claims 3
• 230000001939 inductive effect Effects 0.000 claims 3
• 102000008143 Bone Morphogenetic Protein 2 Human genes 0.000 claims 2
• 108010049931 Bone Morphogenetic Protein 2 Proteins 0.000 claims 2
• 102000000503 Collagen Type II Human genes 0.000 claims 2
• 108010041390 Collagen Type II Proteins 0.000 claims 2
• 102000018233 Fibroblast Growth Factor Human genes 0.000 claims 2
• 108050007372 Fibroblast Growth Factor Proteins 0.000 claims 2
• 241000233866 Fungi Species 0.000 claims 2
• 241000282414 Homo sapiens Species 0.000 claims 2
• 102000004877 Insulin Human genes 0.000 claims 2
• 108090001061 Insulin Proteins 0.000 claims 2
• 230000003321 amplification Effects 0.000 claims 2
• 229940126864 fibroblast growth factor Drugs 0.000 claims 2
• 229940125396 insulin Drugs 0.000 claims 2
• 239000002609 medium Substances 0.000 claims 2
• 238000003199 nucleic acid amplification method Methods 0.000 claims 2
• 239000002243 precursor Substances 0.000 claims 2
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
• 235000017166 Bambusa arundinacea Nutrition 0.000 claims 1
• 235000017491 Bambusa tulda Nutrition 0.000 claims 1
• 241001330002 Bambuseae Species 0.000 claims 1
• 241000282465 Canis Species 0.000 claims 1
• 239000004971 Cross linker Substances 0.000 claims 1
• 241000283073 Equus caballus Species 0.000 claims 1
• 235000015334 Phyllostachys viridis Nutrition 0.000 claims 1
• AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 claims 1
• 210000003157 atrial septum Anatomy 0.000 claims 1
• 230000001580 bacterial effect Effects 0.000 claims 1
• 239000011425 bamboo Substances 0.000 claims 1
• 210000004271 bone marrow stromal cell Anatomy 0.000 claims 1
• 230000003833 cell viability Effects 0.000 claims 1
• 230000001413 cellular effect Effects 0.000 claims 1
• 239000003431 cross linking reagent Substances 0.000 claims 1
• 210000001671 embryonic stem cell Anatomy 0.000 claims 1
• 238000000855 fermentation Methods 0.000 claims 1
• 230000004151 fermentation Effects 0.000 claims 1
• 238000001879 gelation Methods 0.000 claims 1
• 230000012010 growth Effects 0.000 claims 1
• 239000001963 growth medium Substances 0.000 claims 1
• 210000003035 hyaline cartilage Anatomy 0.000 claims 1
• 238000000338 in vitro Methods 0.000 claims 1
• 210000002901 mesenchymal stem cell Anatomy 0.000 claims 1
• 108020004999 messenger RNA Proteins 0.000 claims 1
• 210000000492 nasalseptum Anatomy 0.000 claims 1
• 238000010899 nucleation Methods 0.000 claims 1
• 229920000642 polymer Polymers 0.000 claims 1
• 230000002194 synthesizing effect Effects 0.000 claims 1
• 230000017423 tissue regeneration Effects 0.000 claims 1
• 229940035722 triiodothyronine Drugs 0.000 claims 1