JP6821583B2 - キトサン及び軟骨細胞を含む軟骨修復のための軟骨ゲル - Google Patents

キトサン及び軟骨細胞を含む軟骨修復のための軟骨ゲル Download PDF

Info

Publication number
JP6821583B2
JP6821583B2 JP2017547081A JP2017547081A JP6821583B2 JP 6821583 B2 JP6821583 B2 JP 6821583B2 JP 2017547081 A JP2017547081 A JP 2017547081A JP 2017547081 A JP2017547081 A JP 2017547081A JP 6821583 B2 JP6821583 B2 JP 6821583B2
Authority
JP
Japan
Prior art keywords
chitosan
cells
hydrogel
chondrocytes
particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017547081A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017535405A5 (https=
JP2017535405A (ja
Inventor
パスカル・アゾ
フレデリック・マラン・ジェラン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Publication of JP2017535405A publication Critical patent/JP2017535405A/ja
Publication of JP2017535405A5 publication Critical patent/JP2017535405A5/ja
Application granted granted Critical
Publication of JP6821583B2 publication Critical patent/JP6821583B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/225—Fibrin; Fibrinogen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3612—Cartilage, synovial fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3645—Connective tissue
    • A61L27/3654—Cartilage, e.g. meniscus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/56—Fibrin; Thrombin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Rheumatology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2017547081A 2014-12-01 2015-12-01 キトサン及び軟骨細胞を含む軟骨修復のための軟骨ゲル Active JP6821583B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1461746 2014-12-01
FR1461746A FR3029116B1 (fr) 2014-12-01 2014-12-01 Procede d'obtention d'un gel de cartilage pour la reparation cartilagineuse, comprenant du chitosane et des chondrocytes
PCT/FR2015/053271 WO2016087762A1 (fr) 2014-12-01 2015-12-01 Gel de cartilage pour la réparation cartilagineuse, comprenant du chitosane et des chondrocytes

Publications (3)

Publication Number Publication Date
JP2017535405A JP2017535405A (ja) 2017-11-30
JP2017535405A5 JP2017535405A5 (https=) 2019-01-17
JP6821583B2 true JP6821583B2 (ja) 2021-01-27

Family

ID=53177537

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017547081A Active JP6821583B2 (ja) 2014-12-01 2015-12-01 キトサン及び軟骨細胞を含む軟骨修復のための軟骨ゲル

Country Status (9)

Country Link
US (2) US10612001B2 (https=)
EP (1) EP3226923B1 (https=)
JP (1) JP6821583B2 (https=)
CA (1) CA2969285C (https=)
DK (1) DK3226923T3 (https=)
ES (1) ES2870505T3 (https=)
FR (2) FR3029116B1 (https=)
PT (1) PT3226923T (https=)
WO (1) WO2016087762A1 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3038318B1 (fr) * 2015-07-02 2017-08-04 Univ De Lille 1 Sciences Et Technologies Procede de fabrication d'hydrogel a base de chitosan et de polyelectrolytes charges negativement et materiau poreux alveolaire issu dudit hydrogel
US10898608B2 (en) 2017-02-02 2021-01-26 Nanofiber Solutions, Llc Methods of improving bone-soft tissue healing using electrospun fibers
EP3595515A4 (en) 2017-03-14 2020-12-30 University of Connecticut BIODEGRADABLE PRESSURE SENSOR
FR3074043B1 (fr) 2017-11-28 2020-11-13 Kiomed Pharma Chitosane a charge anionique
FR3074044B1 (fr) 2017-11-28 2019-11-01 Kiomed Pharma Carboxyalkyl chitosane
WO2019113511A1 (en) * 2017-12-08 2019-06-13 Cohen Brian S Electrospun fibers for the repair and regrowth of hyaline cartilage
CN108774330B (zh) * 2018-08-30 2020-11-10 华熙生物科技股份有限公司 一种交联透明质酸凝胶的制备方法及所得产品和应用
JP2022513442A (ja) 2018-12-11 2022-02-08 ナノファイバー ソリューションズ、エルエルシー 電界紡糸繊維を使用した慢性創傷の治療方法
US11826495B2 (en) 2019-03-01 2023-11-28 University Of Connecticut Biodegradable piezoelectric ultrasonic transducer system
KR102197871B1 (ko) * 2019-03-28 2021-01-04 이화여자대학교 산학협력단 엽산, 엽산 유도체, 또는 엽산 저해제를 이용한 줄기세포의 연골세포로의 분화 유도 방법
KR102337350B1 (ko) * 2019-11-22 2021-12-08 아주대학교산학협력단 내재성 전구세포 또는 줄기세포 포집능 및 포집된 세포의 혈관 분화 유도능을 갖는 주입형 하이드로겔 조성물
US11745001B2 (en) 2020-03-10 2023-09-05 University Of Connecticut Therapeutic bandage
US12491290B2 (en) 2020-06-08 2025-12-09 University Of Connecticut Biodegradable piezoelectric composite materials
FR3116534B1 (fr) 2020-11-23 2023-12-22 Kiomed Pharma Billes à base de chitosane, préparation, compositions et applications
US20230404765A1 (en) * 2020-12-07 2023-12-21 University Of Connecticut A system and method for osteoarthritis treatment
CN114652895A (zh) * 2020-12-23 2022-06-24 上海其胜生物制剂有限公司 一种可注射组织再生型壳聚糖复合凝胶支架的制备方法
CN113663085B (zh) * 2021-08-25 2023-12-15 福州市大福瑞生物科技有限公司 基于卤代环氧烷烃接枝制备皂苷-壳聚糖衍生物的方法及其应用
CN114908038B (zh) * 2022-06-06 2024-03-15 苏州大学 一种体外3d关节损伤模型及其构建与应用
CN115154673B (zh) * 2022-06-28 2023-07-14 北京航空航天大学宁波创新研究院 人工软骨植入物预成型体及制备方法和应用、类生人工软骨植入物胶原纤维原位构建方法
PL443403A1 (pl) 2023-01-03 2024-07-08 Politechnika Gdańska Hydrożelowy kompozyt na bazie chitozanu, sposób wytwarzania hydrożelowego kompozytu na bazie chitozanu oraz zastosowanie kompozytu w postaci biotuszu do tworzenia przestrzennych obiektów, zwłaszcza z wykorzystaniem technologii wytwarzania addytywnego, zwłaszcza celem prowadzenia hodowli komórkowych

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3097999A (en) 1998-03-18 1999-10-11 University Of Pittsburgh Chitosan-based composite materials containing glycosaminoglycan for cartilage repair
KR100375422B1 (ko) * 1999-12-21 2003-03-10 한국과학기술연구원 다공성 키토산 구슬 및 그의 제조 방법
JP2002209573A (ja) * 2001-01-15 2002-07-30 Toru Yamazaki 細胞及び又は生体組織の担持体と移植体、及び担持方法
JP3616344B2 (ja) * 2001-03-29 2005-02-02 紳一郎 西村 軟骨細胞培養方法および軟骨組織再生基材
WO2002078761A1 (fr) 2001-03-30 2002-10-10 Laboratoires Genevrier Neo-tissu cartilagineux greffable
FR2822842B1 (fr) * 2001-03-30 2004-11-19 Genevrier Lab Procede de preparation d'un neo-tissu cartilagineux
US8173171B2 (en) * 2003-11-17 2012-05-08 Taiwan Textile Research Institute Hydrogel microparticle composition, application thereof and method for preparing the same
US7524514B2 (en) * 2003-12-01 2009-04-28 Tissue Engineering Consultants, Inc. Biomimetic composition reinforced by a polyelectrolytic complex of hyaluronic acid and chitosan
CN101098718A (zh) * 2004-12-20 2008-01-02 卡博格拉夫特有限公司 壳聚糖组合物
SG10201500237TA (en) 2008-01-30 2015-04-29 Histogen Inc Extracellular matrix compositions
EP2361640A1 (en) * 2010-02-25 2011-08-31 Université de Liège Cell cultivation in chitosan alginate hydrogel beads
FR2963351B1 (fr) 2010-07-30 2013-03-08 Univ Claude Bernard Lyon Particules formees d'un complexe polyelectrolyte de chitosane et d'un polysaccharide anionique, presentant une stabilite amelioree
FR2965278B1 (fr) 2010-09-23 2014-10-10 Univ Caen Basse Normandie Procede d'obtention in vitro ou ex vivo de chondrocytes et leurs utilisations

Also Published As

Publication number Publication date
DK3226923T3 (da) 2021-05-17
EP3226923B1 (fr) 2021-02-17
FR3062064B1 (fr) 2020-11-27
ES2870505T3 (es) 2021-10-27
CA2969285C (fr) 2023-09-05
US10612001B2 (en) 2020-04-07
US11718828B2 (en) 2023-08-08
US20200248144A1 (en) 2020-08-06
JP2017535405A (ja) 2017-11-30
WO2016087762A1 (fr) 2016-06-09
US20170306295A1 (en) 2017-10-26
FR3062064A1 (fr) 2018-07-27
EP3226923A1 (fr) 2017-10-11
PT3226923T (pt) 2021-05-07
FR3029116A1 (fr) 2016-06-03
CA2969285A1 (fr) 2016-06-09
FR3029116B1 (fr) 2018-03-30

Similar Documents

Publication Publication Date Title
JP6821583B2 (ja) キトサン及び軟骨細胞を含む軟骨修復のための軟骨ゲル
JP7312761B2 (ja) インビトロ培養及び移植のための組織構築物の生理学的３ｄバイオプリンティングのためのバイオガム及び植物性ガムハイドロゲルバイオインク
Yamane et al. Effect of pore size on in vitro cartilage formation using chitosan‐based hyaluronic acid hybrid polymer fibers
Guo et al. Hydrogels of collagen/chondroitin sulfate/hyaluronan interpenetrating polymer network for cartilage tissue engineering
US9180166B2 (en) Cartilage repair systems and applications utilizing a glycosaminoglycan mimic
US20120189588A1 (en) Compositions and Methods for Implantation of Processed Adipose Tissue and Processed Adipose Tissue Products
CN106999635A (zh) 软骨修复用移植物支架及其制造方法
US20250065008A1 (en) Composition containing mesenchymal stem cells and hydrogel, and use thereof
Wan et al. BMSCs laden injectable amino-diethoxypropane modified alginate-chitosan hydrogel for hyaline cartilage reconstruction
WO2013025763A2 (en) Tissue engineering using injectable, oxidized alginate hydrogels
Song et al. Injectable hyaluronate/collagen hydrogel with enhanced safety and efficacy for facial rejuvenation
CN101920045B (zh) 一种明胶-壳聚糖-透明质酸-硫酸肝素复合三维支架及其制备方法
CN116535682A (zh) 水凝胶生物材料及其制备方法和应用
WO2007129828A1 (en) Preparation method of porous hyaluronic acid sponge for cell delivery system
US20120003176A1 (en) Hydrogel-forming composition comprising natural and synthetic segments
De Angelis et al. The in vitro biocompatibility of D-(+) raffinose modified chitosan: Two-dimensional and three-dimensional systems for culturing of horse articular chondrocytes
CN104491925B (zh) 一种复合骨髓间充质干细胞的凝胶支架移植系统及其应用
WO2021255123A1 (en) Matured three-dimensional printed compositions and uses thereof
KR20250030639A (ko) 주사 가능한 단일 성분 하이드로젤
Kang Biofabricated platforms for wound healing and skin regeneration
US20120003192A1 (en) Hydrogel-forming composition comprising protein and non-protein segments
Zidarič et al. Growing Skin-Like Tissue
de Carvalho Development of Hyaluronic Acid, Dextrin and Extracellular Matrix Hydrogels for Cell Expansion
WO2026079021A1 (ja) ゲル組成物
Kwon Engineering The Interface: Hyaluronic Acid Hydrogels That Mediate Msc Chondrogenesis For Cartilage Tissue Engineering

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181128

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20181128

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190924

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20190920

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191224

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200420

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200720

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20201207

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210106

R150 Certificate of patent or registration of utility model

Ref document number: 6821583

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250