JP2017530714A5 - - Google Patents

Download PDF

Info

Publication number
JP2017530714A5
JP2017530714A5 JP2017519821A JP2017519821A JP2017530714A5 JP 2017530714 A5 JP2017530714 A5 JP 2017530714A5 JP 2017519821 A JP2017519821 A JP 2017519821A JP 2017519821 A JP2017519821 A JP 2017519821A JP 2017530714 A5 JP2017530714 A5 JP 2017530714A5
Authority
JP
Japan
Prior art keywords
polynucleotide
double
modified
stranded
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2017519821A
Other languages
English (en)
Japanese (ja)
Other versions
JP6637040B2 (ja
JP2017530714A (ja
Filing date
Publication date
Priority claimed from GBGB1418159.8A external-priority patent/GB201418159D0/en
Application filed filed Critical
Publication of JP2017530714A publication Critical patent/JP2017530714A/ja
Publication of JP2017530714A5 publication Critical patent/JP2017530714A5/ja
Application granted granted Critical
Publication of JP6637040B2 publication Critical patent/JP6637040B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2017519821A 2014-10-14 2015-02-18 方法 Active JP6637040B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1418159.8 2014-10-14
GBGB1418159.8A GB201418159D0 (en) 2014-10-14 2014-10-14 Method
PCT/GB2015/050461 WO2016059363A1 (en) 2014-10-14 2015-02-18 Method

Publications (3)

Publication Number Publication Date
JP2017530714A JP2017530714A (ja) 2017-10-19
JP2017530714A5 true JP2017530714A5 (OSRAM) 2018-03-29
JP6637040B2 JP6637040B2 (ja) 2020-01-29

Family

ID=52001384

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017519821A Active JP6637040B2 (ja) 2014-10-14 2015-02-18 方法

Country Status (7)

Country Link
US (3) US10570440B2 (OSRAM)
EP (1) EP3207152B1 (OSRAM)
JP (1) JP6637040B2 (OSRAM)
KR (1) KR102280161B1 (OSRAM)
CN (1) CN107109483B (OSRAM)
GB (1) GB201418159D0 (OSRAM)
WO (1) WO2016059363A1 (OSRAM)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010209528B2 (en) 2009-01-30 2015-10-01 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
WO2013014451A1 (en) 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US9758823B2 (en) 2011-10-21 2017-09-12 Oxford Nanopore Technologies Limited Enzyme method
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
WO2014013260A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Modified helicases
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3325621B1 (en) * 2015-07-17 2021-06-30 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Cloning of single-stranded nucleic acid
US11514289B1 (en) 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
GB201609220D0 (en) * 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2017203268A1 (en) * 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN107488656B (zh) 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
US10370701B2 (en) 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
EP3655547A4 (en) 2017-07-18 2021-03-24 Pacific Biosciences Of California, Inc. METHODS AND COMPOSITIONS FOR ISOLATING ASYMMETRICAL NUCLEIC ACID COMPLEXES
GB2589159B (en) 2017-12-29 2023-04-05 Clear Labs Inc Nucleic acid sequencing apparatus
GB2569977A (en) * 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) * 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201917060D0 (en) * 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
WO2021152147A1 (en) * 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Closed linear dna with modified nucleotides
US20240240248A1 (en) 2021-05-19 2024-07-18 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
CN119095960A (zh) 2022-05-18 2024-12-06 牛津纳米孔科技公开有限公司 用于核酸提取和纯化的组合物和方法
CN118241319A (zh) * 2022-12-22 2024-06-25 北京齐碳科技有限公司 测序文库的制备方法
WO2025067293A1 (zh) * 2023-09-27 2025-04-03 北京齐碳科技有限公司 解旋酶及其应用

Family Cites Families (166)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE56026B1 (en) 1982-10-19 1991-03-27 Cetus Corp Cysteine-depleted muteins of biologically active proteins
GB8924338D0 (en) 1989-10-28 1989-12-13 Atomic Energy Authority Uk Electrodes
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
FR2703693B1 (fr) 1993-04-06 1995-07-13 Pasteur Institut Procédé rapide de détermination d'une séquence d'ADN et application au séquençage et au diagnostic.
CA2160909A1 (en) 1993-04-28 1994-11-10 Hagan Bayley Cell-targeted lytic pore-forming agents
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
DE4320201A1 (de) 1993-06-18 1995-01-12 Asta Medica Ag Verwendung von Cetrorelix und weiteren Nona- und Dekapeptiden zur Herstellung eines Arzneimittels zur Bekämpfung von Aids und zur Wachstumsstimulation
US5561043A (en) 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US7569341B2 (en) 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
DE19648625A1 (de) * 1996-11-13 1998-05-14 Soft Gene Gmbh Mikroprojektil für das Einbringen von Substanzen in Zellen durch ballistischen Transfer
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型rna−dnaキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU5763000A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Molecular and atomic scale evaluation of biopolymers
WO2001002425A2 (en) 1999-06-29 2001-01-11 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
US6682649B1 (en) 1999-10-01 2004-01-27 Sophion Bioscience A/S Substrate and a method for determining and/or monitoring electrophysiological properties of ion channels
AU1804001A (en) 1999-12-02 2001-06-12 Molecular Staging, Inc. Generation of single-strand circular dna from linear self-annealing segments
WO2001059453A2 (en) 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
WO2001070174A2 (en) 2000-03-21 2001-09-27 Curagen Corporation Vegf-modulated genes and methods employing them
EP1265914B1 (en) 2000-03-22 2007-12-26 Curagen Corporation Wnt-1 related polypeptides, and nucleic acids encoding the same
US6596488B2 (en) 2000-03-30 2003-07-22 City Of Hope Tumor suppressor gene
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6936433B2 (en) 2000-11-27 2005-08-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US20030087232A1 (en) 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
AU2002320294B2 (en) 2001-07-03 2009-01-15 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mammalian sweet and amino acid heterodimeric taste receptors
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
IL163822A0 (en) 2002-03-15 2005-12-18 Nuevolution As An improved method for synthesising templated molecules
AU2003245272A1 (en) 2002-05-10 2003-11-11 The Texas A And M University System Stochastic sensing through covalent interactions
WO2004065582A2 (en) 2003-01-15 2004-08-05 Dana-Farber Cancer Institute, Inc. Amplification of dna in a hairpin structure, and applications
WO2004072294A2 (en) 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
US7344882B2 (en) 2003-05-12 2008-03-18 Bristol-Myers Squibb Company Polynucleotides encoding variants of the TRP channel family member, LTRPC3
US20070122885A1 (en) 2005-08-22 2007-05-31 Fermalogic, Inc. Methods of increasing production of secondary metabolites by manipulating metabolic pathways that include methylmalonyl-coa
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
GB2413796B (en) 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US20050227239A1 (en) 2004-04-08 2005-10-13 Joyce Timothy H Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes
WO2005118877A2 (en) 2004-06-02 2005-12-15 Vicus Bioscience, Llc Producing, cataloging and classifying sequence tags
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US7972858B2 (en) 2004-08-13 2011-07-05 President And Fellows Of Harvard College Ultra high-throughput opti-nanopore DNA readout platform
US20060086626A1 (en) 2004-10-22 2006-04-27 Joyce Timothy H Nanostructure resonant tunneling with a gate voltage source
EP1842061A4 (en) 2004-12-21 2009-05-13 Texas A & M Univ Sys HIGH-TEMPERATURE ION CHANNELS AND ION PORES
US7890268B2 (en) 2004-12-28 2011-02-15 Roche Molecular Systems, Inc. De-novo sequencing of nucleic acids
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7507575B2 (en) 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
EP1910537A1 (en) 2005-06-06 2008-04-16 454 Life Sciences Corporation Paired end sequencing
WO2007018601A1 (en) * 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
ATE518010T1 (de) 2005-12-22 2011-08-15 Pacific Biosciences California Aktive oberflächengekoppelte polymerasen
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
US20070224613A1 (en) 2006-02-18 2007-09-27 Strathmann Michael P Massively Multiplexed Sequencing
US8673567B2 (en) 2006-03-08 2014-03-18 Atila Biosystems, Inc. Method and kit for nucleic acid sequence detection
DK3239304T3 (da) 2006-04-04 2020-10-26 Keygene Nv Højgennemløbsdetektering af molekylære markører baseret på AFLP og sekventering med højt gennemløb
CN101495656B (zh) 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
JP4876766B2 (ja) 2006-08-10 2012-02-15 トヨタ自動車株式会社 燃料電池
AU2007292295B2 (en) 2006-09-06 2013-06-06 Immunoforge Co., Ltd. Fusion peptide therapeutic compositions
WO2008045575A2 (en) 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
GB2445016B (en) 2006-12-19 2012-03-07 Microsaic Systems Plc Microengineered ionisation device
DK2122344T3 (da) 2007-02-20 2019-07-15 Oxford Nanopore Tech Ltd Lipiddobbeltlags-sensorsystem
KR101521990B1 (ko) 2007-04-04 2015-05-20 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 나노포어 사용을 위한 조성물, 장치, 시스템 및 방법
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
EP2232233B1 (en) 2007-12-31 2014-03-12 Fujirebio Inc. Clusters of microresonators for cavity mode optical sensing
US8852864B2 (en) 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
WO2009120372A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CN102066579A (zh) 2008-04-24 2011-05-18 纽约市哥伦比亚大学理事会 用于dna分子编排的几何图案和脂质双层以及它们的用途
CN103695530B (zh) 2008-07-07 2016-05-25 牛津纳米孔技术有限公司 酶-孔构建体
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
CA3092369C (en) 2008-09-22 2025-09-23 University Of Washington MSP NANOPORES AND ASSOCIATED PROCESSES
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CN105018548B (zh) 2008-10-24 2019-03-12 阿霹震中科技公司 用于修饰核酸的试剂盒
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8486630B2 (en) 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
WO2010068289A2 (en) 2008-12-11 2010-06-17 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
AU2010209528B2 (en) 2009-01-30 2015-10-01 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
CA2750874A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
SG173105A1 (en) 2009-02-16 2011-09-29 Epict Technologies Corp Template-independent ligation of single-stranded dna
RU2011138951A (ru) 2009-02-23 2013-03-27 Сайтомкс Терапьютикс, Инк. Пропротеины и способы их применения
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
FR2943656A1 (fr) 2009-03-25 2010-10-01 Air Liquide Procede et installation de production d'hydrogene mettant en oeuvre un compresseur thermocinetique
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
BRPI1012752B1 (pt) 2009-04-20 2019-06-25 Oxford Nanopore Technologies Limited Método e aparelho para detectar uma interação de uma entidade molecular com uma proteína de membrana em uma camada de moléculas anfifílicas
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
WO2011090556A1 (en) 2010-01-19 2011-07-28 Verinata Health, Inc. Methods for determining fraction of fetal nucleic acid in maternal samples
FR2955773B1 (fr) 2010-02-01 2017-05-26 Commissariat Energie Atomique Complexe moleculaire de ciblage des antigenes vers les cellules presentatrices d'antigene et ses applications pour la vaccination
KR20110100963A (ko) 2010-03-05 2011-09-15 삼성전자주식회사 미세 유동 장치 및 이를 이용한 표적 핵산의 염기 서열 결정 방법
EP2545183B1 (en) 2010-03-10 2017-04-19 Ibis Biosciences, Inc. Production of single-stranded circular nucleic acid
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
CN103392008B (zh) 2010-09-07 2017-10-20 加利福尼亚大学董事会 通过持续性酶以一个核苷酸的精度控制dna在纳米孔中的移动
AU2011323107B2 (en) 2010-11-05 2015-09-10 Illumina, Inc. Linking sequence reads using paired code tags
EP2652153B1 (en) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
US20130291392A1 (en) 2011-01-18 2013-11-07 R.K. Swamy Multipurpose instrument for triangle solutions, measurements and geometrical applications called triometer
US9402808B2 (en) 2011-01-19 2016-08-02 Panacea Biotec Limited Liquid oral composition of lanthanum salts
DK3037536T3 (da) 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
EP2673638B1 (en) 2011-02-11 2019-10-30 Oxford Nanopore Technologies Limited Mutant pores
WO2012164270A1 (en) 2011-05-27 2012-12-06 Oxford Nanopore Technologies Limited Coupling method
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
WO2013014451A1 (en) 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
WO2013041878A1 (en) 2011-09-23 2013-03-28 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US20140308661A1 (en) 2011-09-25 2014-10-16 Theranos, Inc. Systems and methods for multi-analysis
US9758823B2 (en) 2011-10-21 2017-09-12 Oxford Nanopore Technologies Limited Enzyme method
US9404147B2 (en) * 2011-12-19 2016-08-02 Gen-Probe Incorporated Closed nucleic acid structures
CN104136631B (zh) 2011-12-29 2017-03-01 牛津纳米孔技术公司 使用xpd解旋酶表征多核苷酸的方法
US10385382B2 (en) 2011-12-29 2019-08-20 Oxford Nanopore Technologies Ltd. Enzyme method
NO2694769T3 (OSRAM) 2012-03-06 2018-03-03
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
WO2013185137A1 (en) * 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
TWI655213B (zh) 2012-07-13 2019-04-01 目立康股份有限公司 自我組織化肽衍生物的製造方法
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
WO2014013260A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Modified helicases
AU2013291765C1 (en) 2012-07-19 2019-08-08 Oxford Nanopore Technologies Limited Enzyme construct
EP2895618B1 (en) 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
EP2917732B1 (en) 2012-11-09 2016-09-07 Stratos Genomics Inc. Concentrating a target molecule for sensing by a nanopore
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US20140206842A1 (en) 2013-01-22 2014-07-24 Muhammed Majeed Peptides Modified with Triterpenoids and Small Organic Molecules: Synthesis and use in Cosmeceutical
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
KR102168813B1 (ko) * 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
US9567632B2 (en) 2013-03-19 2017-02-14 New England Biolabs, Inc. Enrichment of target sequences
WO2015031909A1 (en) 2013-08-30 2015-03-05 University Of Washington Through Its Center For Commercialization Selective modification of polymer subunits to improve nanopore-based analysis
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201321123D0 (en) * 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
CN106103741B (zh) 2014-01-22 2020-03-13 牛津纳米孔技术公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015148567A1 (en) 2014-03-24 2015-10-01 Bernick David L Molecular adapter for capture and manipulation of transfer rna
CA3172086A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
WO2016022557A1 (en) 2014-08-05 2016-02-11 Twist Bioscience Corporation Cell free cloning of nucleic acids
WO2016028887A1 (en) 2014-08-19 2016-02-25 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
US10612073B2 (en) 2015-02-26 2020-04-07 Hitachi High-Technologies Corporation Method for constructing nucleic acid molecule
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN107488656B (zh) 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method

Similar Documents

Publication Publication Date Title
JP2017530714A5 (OSRAM)
JP2012516146A5 (OSRAM)
JP2017503517A5 (OSRAM)
JP2017532962A5 (OSRAM)
US10023906B2 (en) Method for constructing nucleic acid single-stranded cyclic library and reagents thereof
US20200239936A1 (en) Compositions, methods, systems and kits for target nucleic acid enrichment
CL2021002742A1 (es) Una proteína crispr de prevotella y francisella 1 (cpf1) aislada de francisella tularensis; proteína de fusión que la comprende; ácido nucleico; vector; métodos para alterar el genoma de una célula o una molécula de adn bicatenario in vitro que utilizan dichas proteínas (divisional de sol. 201903004)
WO2014108850A3 (en) High throughput transcriptome analysis
WO2018119447A3 (en) Methods and systems for processing polynucleotides
MY149161A (en) Methods using pores
HRP20211078T1 (hr) Metoda za molekularno kodiranje nukleinskih kiselina pojedinačnih stanica
JP2016537008A5 (OSRAM)
WO2010086622A8 (en) Adaptors for nucleic acid constructs in transmembrane sequencing
WO2015083001A3 (en) Nucleic acid probe and method of detecting genomic fragments
JP2016508715A5 (OSRAM)
HK1250245A1 (zh) 生物样本的空间区别、多重核酸分析
WO2011155833A3 (en) Combinatorial sequence barcodes for high throughput screening
WO2014152397A3 (en) Selective purification of rna and rna-bound molecular complexes
EP4353830A3 (en) Methods of nucleic acid sample preparation for analysis of cell-free dna
WO2013173774A3 (en) Molecular inversion probes
WO2017201102A8 (en) Method of improved sequencing by strand identification
CN106282353A (zh) 一种利用发夹引物进行多重pcr的方法
RU2019130498A (ru) Способ репликации или амплификации кольцевой днк
EP3250716A1 (en) Microscope-free imaging
WO2019117714A8 (en) Methods for preparing nucleic acid molecules for sequencing