JP2017527299A - 灌流バイオリアクター細胞保持のためのマイクロ流体システム及び方法 - Google Patents

灌流バイオリアクター細胞保持のためのマイクロ流体システム及び方法 Download PDF

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JP2017527299A
JP2017527299A JP2017514828A JP2017514828A JP2017527299A JP 2017527299 A JP2017527299 A JP 2017527299A JP 2017514828 A JP2017514828 A JP 2017514828A JP 2017514828 A JP2017514828 A JP 2017514828A JP 2017527299 A JP2017527299 A JP 2017527299A
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curved
reaction mixture
cells
microchannel
biological reaction
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JP2017527299A5 (enExample
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ワーキアニ、マジド イブラヒミ
ワーキアニ、マジド イブラヒミ
ハン、チョンユン
ピン、アンディ テイ、カー
ピン、アンディ テイ、カー
コアン、クオフォン
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Massachusetts Institute of Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2017514828A 2014-09-17 2015-09-17 灌流バイオリアクター細胞保持のためのマイクロ流体システム及び方法 Pending JP2017527299A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462051497P 2014-09-17 2014-09-17
US62/051,497 2014-09-17
PCT/US2015/050604 WO2016044537A1 (en) 2014-09-17 2015-09-17 Microfluidic system and method for perfusion bioreactor cell retention

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JP2017527299A true JP2017527299A (ja) 2017-09-21
JP2017527299A5 JP2017527299A5 (enExample) 2018-10-25

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US (1) US20170292104A1 (enExample)
EP (1) EP3194560A4 (enExample)
JP (1) JP2017527299A (enExample)
CN (1) CN107250341A (enExample)
WO (1) WO2016044537A1 (enExample)

Cited By (4)

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JP2019122363A (ja) * 2018-01-17 2019-07-25 株式会社Ihi 細胞培養システム及び細胞培養方法
WO2019240222A1 (ja) * 2018-06-13 2019-12-19 株式会社Ihi 細胞培養システム及び細胞培養方法
WO2021010254A1 (ja) * 2019-07-12 2021-01-21 株式会社Ihi 微生物選別システム及び微生物選別方法
WO2024062903A1 (ja) * 2022-09-21 2024-03-28 キヤノン株式会社 マイクロ流路デバイス、分離装置、および分離方法

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EP3628401B1 (en) 2012-09-21 2021-06-23 Massachusetts Institute Of Technology Micro-fluidic device and uses thereof
SG11201606832YA (en) 2014-02-18 2016-09-29 Massachusetts Inst Technology Biophysically sorted osteoprogenitors from culture expanded bone marrow derived mesenchymal stromal cells (mscs)
WO2016044555A1 (en) 2014-09-17 2016-03-24 Massachusetts Institute Of Technology System and method for inertial focusing microfiltration for intra-operative blood salvage autotransfusion
JP6912161B2 (ja) * 2016-02-25 2021-07-28 株式会社神戸製鋼所 流路装置及び液滴形成方法
US20190275521A1 (en) 2016-07-21 2019-09-12 Agency For Science, Technology And Research Apparatus for outer wall focusing for high volume fraction particle microfiltration and method for manufacture thereof
EP3523003B1 (en) * 2016-10-07 2023-09-13 Massachusetts Institute of Technology Particle isolation/enrichment using continuous closed-loop micro-fluidics
US10697964B2 (en) 2016-11-15 2020-06-30 Massachusetts Institute Of Technology Liquid biopsy detection of leukemia using closed-loop microfluidics
CN108435265A (zh) * 2017-01-25 2018-08-24 富士康(昆山)电脑接插件有限公司 微流体盒及其堆叠测试组件
KR101901672B1 (ko) 2017-01-25 2018-10-25 주식회사 디오스템스 줄기세포 배양기 및 배양 방법, 줄기세포 연속 배양, 추출 및 분리 시스템 및 방법
JP2020513798A (ja) * 2017-03-17 2020-05-21 イスタンブール・テクニック・ユニヴェルシテシＩｓｔａｎｂｕｌ Ｔｅｋｎｉｋ Ｕｎｉｖｅｒｓｉｔｅｓｉ ガン細胞の血液からの連続的分離および循環系でのその豊富化のための非対称カールにより構成されるらせん形状を有するマイクロチャネル
CN106994369A (zh) * 2017-05-22 2017-08-01 东南大学 通量可调控的微流控集成器件及其制作方法
CN108517284A (zh) * 2018-03-19 2018-09-11 复旦大学 一种用于研究藻类生长和生殖的微流控芯片
TWI898645B (zh) 2018-05-04 2025-09-21 美商健臻公司 灌注式細胞培養裝置
US20210163868A1 (en) * 2018-05-22 2021-06-03 Nantkwest, Inc. Methods and systems for cell bed formation during bioprocessing
EP4477315A3 (en) 2018-05-31 2025-03-05 Donaldson Company, Inc. Droplet sensors for fuel systems
WO2019245070A1 (ko) * 2018-06-20 2019-12-26 주식회사 디오스템스 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법
WO2020102533A1 (en) 2018-11-15 2020-05-22 Massachusetts Institute Of Technology Multi-dimensional double spiral device and methods of use thereof
CN111774103B (zh) * 2020-06-01 2021-09-07 东南大学 一种高通量血浆分离的多核螺旋惯性分选微流控器件
SE544733C2 (en) * 2020-07-20 2022-10-25 Javier Cruz Microfluidic device and method for manipulation of particles in fluids
US20240002772A1 (en) * 2020-10-13 2024-01-04 Boehringer Ingelheim International Gmbh Systems and methods for producing a biologic molecule with hydrocyclone cell retention device
KR102474060B1 (ko) * 2020-10-14 2022-12-05 한국과학기술연구원 미세유체칩 유동에 의한 세포입자의 크기별 분리 장치와 방법
EP4251296B1 (en) * 2020-11-25 2025-06-25 Donaldson Company, Inc. Hydrodynamic separator with optimal microchannel length
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US12252678B2 (en) 2021-12-01 2025-03-18 Microfluidx Ltd Systems and methods for bioprocessing
CN114260037B (zh) * 2021-12-27 2023-02-03 杭州电子科技大学 一种3d堆叠式多级惯性微流控分选芯片及其制备方法
WO2023211901A1 (en) 2022-04-28 2023-11-02 Massachusetts Institute Of Technology Spiral inertial microfluidic devices and methods to remove contaminants
CN114813466B (zh) * 2022-05-05 2025-02-18 山东大学 一种预测水力旋流器分选细胞活性的方法及系统

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019122363A (ja) * 2018-01-17 2019-07-25 株式会社Ihi 細胞培養システム及び細胞培養方法
JP2019122362A (ja) * 2018-01-17 2019-07-25 株式会社Ihi 細胞培養システム及び細胞培養方法
WO2019240221A1 (ja) * 2018-01-17 2019-12-19 株式会社Ihi 細胞培養システム及び細胞培養方法
CN112368365A (zh) * 2018-01-17 2021-02-12 株式会社Ihi 细胞培养系统及细胞培养方法
JP7091861B2 (ja) 2018-01-17 2022-06-28 株式会社Ihi 細胞培養システム及び細胞培養方法
JP7124475B2 (ja) 2018-01-17 2022-08-24 株式会社Ihi 細胞培養システム及び細胞培養方法
WO2019240222A1 (ja) * 2018-06-13 2019-12-19 株式会社Ihi 細胞培養システム及び細胞培養方法
JP2019213497A (ja) * 2018-06-13 2019-12-19 株式会社Ihi 細胞培養システム及び細胞培養方法
JP7087704B2 (ja) 2018-06-13 2022-06-21 株式会社Ihi 細胞培養システム及び細胞培養方法
WO2021010254A1 (ja) * 2019-07-12 2021-01-21 株式会社Ihi 微生物選別システム及び微生物選別方法
JP2021013333A (ja) * 2019-07-12 2021-02-12 株式会社Ihi 微生物選別システム及び微生物選別方法
WO2024062903A1 (ja) * 2022-09-21 2024-03-28 キヤノン株式会社 マイクロ流路デバイス、分離装置、および分離方法

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US20170292104A1 (en) 2017-10-12

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