CN107250341A - 用于灌注生物反应器细胞保留的方法及微流体系统 - Google Patents

用于灌注生物反应器细胞保留的方法及微流体系统 Download PDF

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Publication number
CN107250341A
CN107250341A CN201580062211.0A CN201580062211A CN107250341A CN 107250341 A CN107250341 A CN 107250341A CN 201580062211 A CN201580062211 A CN 201580062211A CN 107250341 A CN107250341 A CN 107250341A
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cell
shaped form
microchannel
mixture
form microchannel
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M·埃博拉西米沃齐亚尼
韩宗润
K·P·A·泰
关国峰
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National University of Singapore
Massachusetts Institute of Technology
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Massachusetts Institute of Technology
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices

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  • Life Sciences & Earth Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201580062211.0A 2014-09-17 2015-09-17 用于灌注生物反应器细胞保留的方法及微流体系统 Pending CN107250341A (zh)

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US201462051497P 2014-09-17 2014-09-17
US62/051,497 2014-09-17
PCT/US2015/050604 WO2016044537A1 (en) 2014-09-17 2015-09-17 Microfluidic system and method for perfusion bioreactor cell retention

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US (1) US20170292104A1 (enExample)
EP (1) EP3194560A4 (enExample)
JP (1) JP2017527299A (enExample)
CN (1) CN107250341A (enExample)
WO (1) WO2016044537A1 (enExample)

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CN111774103A (zh) * 2020-06-01 2020-10-16 东南大学 一种高通量血浆分离的多核螺旋惯性分选微流控器件
CN112262209A (zh) * 2018-06-13 2021-01-22 株式会社Ihi 细胞培养系统及细胞培养方法
CN112368365A (zh) * 2018-01-17 2021-02-12 株式会社Ihi 细胞培养系统及细胞培养方法
CN114127254A (zh) * 2019-07-12 2022-03-01 株式会社Ihi 微生物筛选系统和微生物筛选方法
CN114813466A (zh) * 2022-05-05 2022-07-29 山东大学 一种预测水力旋流器分选细胞活性的方法及系统
TWI846694B (zh) * 2018-05-04 2024-07-01 美商健臻公司 具有過濾系統的灌注式生物反應器

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SG11201606832YA (en) 2014-02-18 2016-09-29 Massachusetts Inst Technology Biophysically sorted osteoprogenitors from culture expanded bone marrow derived mesenchymal stromal cells (mscs)
US10806845B2 (en) 2014-09-17 2020-10-20 Massachusetts Institute Of Technology System and method for inertial focusing microfiltration for intra-operative blood salvage autotransfusion
JP6912161B2 (ja) * 2016-02-25 2021-07-28 株式会社神戸製鋼所 流路装置及び液滴形成方法
JP7082453B2 (ja) * 2016-07-21 2022-06-08 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 高体積分率粒子精密濾過のための外壁集束のための装置及びその製造方法
US10697871B2 (en) 2016-10-07 2020-06-30 Massachusetts Institute Of Technology Particle isolation/enrichment using continuous closed-loop micro-fluidics
WO2018093475A1 (en) * 2016-11-15 2018-05-24 Massachusetts Institute Of Technology Liquid biopsy detection of leukemia using closed-loop microfluidics
TWI728225B (zh) * 2017-01-25 2021-05-21 英屬開曼群島商鴻騰精密科技股份有限公司 微流體盒及其堆疊測試組件
KR101901672B1 (ko) 2017-01-25 2018-10-25 주식회사 디오스템스 줄기세포 배양기 및 배양 방법, 줄기세포 연속 배양, 추출 및 분리 시스템 및 방법
DE112018001435T5 (de) * 2017-03-17 2019-12-05 Istanbul Teknik Universitesi Mikrokanal mit aus asymmetrischen Windungen aufgebauter spiralförmiger Geometrie zur kontinuierlichen Abtrennung von Krebszellen aus Blut und Anreicherung derselben im Kreislaufsystem
CN106994369A (zh) * 2017-05-22 2017-08-01 东南大学 通量可调控的微流控集成器件及其制作方法
CN108517284A (zh) * 2018-03-19 2018-09-11 复旦大学 一种用于研究藻类生长和生殖的微流控芯片
US20210163868A1 (en) * 2018-05-22 2021-06-03 Nantkwest, Inc. Methods and systems for cell bed formation during bioprocessing
WO2019232305A1 (en) 2018-05-31 2019-12-05 Donaldson Company, Inc. Droplet sensors for fuel systems
WO2019245070A1 (ko) * 2018-06-20 2019-12-26 주식회사 디오스템스 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법
EP3902630B1 (en) 2018-11-15 2024-09-25 Massachusetts Institute of Technology Multi-dimensional double spiral device and methods of use thereof
SE544733C2 (en) * 2020-07-20 2022-10-25 Javier Cruz Microfluidic device and method for manipulation of particles in fluids
WO2022081511A1 (en) * 2020-10-13 2022-04-21 Boehringer Ingelheim International Gmbh Systems and methods for producing a biologic molecule with hydrocyclone cell retention device
KR102474060B1 (ko) * 2020-10-14 2022-12-05 한국과학기술연구원 미세유체칩 유동에 의한 세포입자의 크기별 분리 장치와 방법
CN116847914A (zh) * 2020-11-25 2023-10-03 唐纳森公司 具有最佳微通道长度的水动力分离器
US12478899B2 (en) 2021-06-02 2025-11-25 Donaldson Company, Inc. Maintenance of hydrodynamic separators
US12252678B2 (en) 2021-12-01 2025-03-18 Microfluidx Ltd Systems and methods for bioprocessing
CN114260037B (zh) * 2021-12-27 2023-02-03 杭州电子科技大学 一种3d堆叠式多级惯性微流控分选芯片及其制备方法
WO2023211901A1 (en) 2022-04-28 2023-11-02 Massachusetts Institute Of Technology Spiral inertial microfluidic devices and methods to remove contaminants
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Cited By (7)

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Publication number Priority date Publication date Assignee Title
CN112368365A (zh) * 2018-01-17 2021-02-12 株式会社Ihi 细胞培养系统及细胞培养方法
TWI846694B (zh) * 2018-05-04 2024-07-01 美商健臻公司 具有過濾系統的灌注式生物反應器
CN112262209A (zh) * 2018-06-13 2021-01-22 株式会社Ihi 细胞培养系统及细胞培养方法
CN114127254A (zh) * 2019-07-12 2022-03-01 株式会社Ihi 微生物筛选系统和微生物筛选方法
CN111774103A (zh) * 2020-06-01 2020-10-16 东南大学 一种高通量血浆分离的多核螺旋惯性分选微流控器件
CN114813466A (zh) * 2022-05-05 2022-07-29 山东大学 一种预测水力旋流器分选细胞活性的方法及系统
CN114813466B (zh) * 2022-05-05 2025-02-18 山东大学 一种预测水力旋流器分选细胞活性的方法及系统

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EP3194560A1 (en) 2017-07-26
JP2017527299A (ja) 2017-09-21
WO2016044537A1 (en) 2016-03-24
EP3194560A4 (en) 2018-06-06

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