JP2017517268A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2017517268A5 JP2017517268A5 JP2016572578A JP2016572578A JP2017517268A5 JP 2017517268 A5 JP2017517268 A5 JP 2017517268A5 JP 2016572578 A JP2016572578 A JP 2016572578A JP 2016572578 A JP2016572578 A JP 2016572578A JP 2017517268 A5 JP2017517268 A5 JP 2017517268A5
- Authority
- JP
- Japan
- Prior art keywords
- controlled
- genetically modified
- gene
- genes
- silencing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims description 41
- 230000002503 metabolic effect Effects 0.000 claims description 28
- 244000005700 microbiome Species 0.000 claims description 26
- 230000014509 gene expression Effects 0.000 claims description 24
- 230000030279 gene silencing Effects 0.000 claims description 22
- 102000004169 proteins and genes Human genes 0.000 claims description 18
- 230000004907 flux Effects 0.000 claims description 16
- 230000002779 inactivation Effects 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 230000002797 proteolythic effect Effects 0.000 claims description 13
- 108091005804 Peptidases Proteins 0.000 claims description 11
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 10
- 239000004365 Protease Substances 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 108091033409 CRISPR Proteins 0.000 claims description 4
- 238000010446 CRISPR interference Methods 0.000 claims description 4
- 230000015556 catabolic process Effects 0.000 claims description 4
- 238000006731 degradation reaction Methods 0.000 claims description 4
- 108020005004 Guide RNA Proteins 0.000 claims description 3
- 230000017854 proteolysis Effects 0.000 claims description 3
- 239000000411 inducer Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims 10
- 238000000855 fermentation Methods 0.000 claims 10
- 230000015572 biosynthetic process Effects 0.000 claims 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 8
- 241000588724 Escherichia coli Species 0.000 claims 7
- 101100174653 Dictyostelium discoideum g6pd-2 gene Proteins 0.000 claims 6
- 102000035195 Peptidases Human genes 0.000 claims 6
- 101150085516 ZWF1 gene Proteins 0.000 claims 6
- 235000018102 proteins Nutrition 0.000 claims 6
- 101150033131 sthA gene Proteins 0.000 claims 6
- 101150078419 zwf gene Proteins 0.000 claims 6
- 101150026856 zwf2 gene Proteins 0.000 claims 6
- 101100161530 Mus musculus Acsbg1 gene Proteins 0.000 claims 5
- 101150106096 gltA gene Proteins 0.000 claims 5
- 101150042350 gltA2 gene Proteins 0.000 claims 5
- 101150040445 lpd gene Proteins 0.000 claims 5
- 101150003321 lpdA gene Proteins 0.000 claims 5
- 101150007808 lpdC gene Proteins 0.000 claims 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 4
- 229910019142 PO4 Inorganic materials 0.000 claims 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 4
- 230000002708 enhancing effect Effects 0.000 claims 4
- 239000011777 magnesium Substances 0.000 claims 4
- 229910052749 magnesium Inorganic materials 0.000 claims 4
- 229910052757 nitrogen Inorganic materials 0.000 claims 4
- 230000037361 pathway Effects 0.000 claims 4
- 239000010452 phosphate Substances 0.000 claims 4
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 4
- 229910052717 sulfur Inorganic materials 0.000 claims 4
- 239000011593 sulfur Substances 0.000 claims 4
- 230000005526 G1 to G0 transition Effects 0.000 claims 3
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 claims 3
- 235000004279 alanine Nutrition 0.000 claims 3
- 230000009368 gene silencing by RNA Effects 0.000 claims 3
- 230000037353 metabolic pathway Effects 0.000 claims 3
- 230000037426 transcriptional repression Effects 0.000 claims 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims 2
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 claims 2
- OFOBLEOULBTSOW-UHFFFAOYSA-N malonic acid group Chemical group C(CC(=O)O)(=O)O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims 2
- 230000006337 proteolytic cleavage Effects 0.000 claims 2
- 230000001568 sexual effect Effects 0.000 claims 2
- 230000007704 transition Effects 0.000 claims 2
- KJTLQQUUPVSXIM-ZCFIWIBFSA-N (R)-mevalonic acid Chemical group OCC[C@](O)(C)CC(O)=O KJTLQQUUPVSXIM-ZCFIWIBFSA-N 0.000 claims 1
- -1 (i) the fabl Proteins 0.000 claims 1
- WTLNOANVTIKPEE-UHFFFAOYSA-N 2-acetyloxypropanoic acid Chemical compound OC(=O)C(C)OC(C)=O WTLNOANVTIKPEE-UHFFFAOYSA-N 0.000 claims 1
- KJTLQQUUPVSXIM-UHFFFAOYSA-N DL-mevalonic acid Natural products OCCC(O)(C)CC(O)=O KJTLQQUUPVSXIM-UHFFFAOYSA-N 0.000 claims 1
- LTYOQGRJFJAKNA-KKIMTKSISA-N Malonyl CoA Natural products S(C(=O)CC(=O)O)CCNC(=O)CCNC(=O)[C@@H](O)C(CO[P@](=O)(O[P@](=O)(OC[C@H]1[C@@H](OP(=O)(O)O)[C@@H](O)[C@@H](n2c3ncnc(N)c3nc2)O1)O)O)(C)C LTYOQGRJFJAKNA-KKIMTKSISA-N 0.000 claims 1
- 101100421548 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) sipB gene Proteins 0.000 claims 1
- OJFDKHTZOUZBOS-CITAKDKDSA-N acetoacetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 OJFDKHTZOUZBOS-CITAKDKDSA-N 0.000 claims 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 1
- 230000003698 anagen phase Effects 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- LTYOQGRJFJAKNA-DVVLENMVSA-N malonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LTYOQGRJFJAKNA-DVVLENMVSA-N 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- 230000001902 propagating effect Effects 0.000 claims 1
- 101150113992 sspB gene Proteins 0.000 claims 1
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012226 gene silencing method Methods 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000002924 silencing RNA Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020033434A JP6920491B2 (ja) | 2014-06-11 | 2020-02-28 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462010574P | 2014-06-11 | 2014-06-11 | |
| US62/010,574 | 2014-06-11 | ||
| PCT/US2015/035306 WO2016053397A2 (en) | 2014-06-11 | 2015-06-11 | Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020033434A Division JP6920491B2 (ja) | 2014-06-11 | 2020-02-28 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017517268A JP2017517268A (ja) | 2017-06-29 |
| JP2017517268A5 true JP2017517268A5 (https=) | 2018-05-10 |
| JP6730199B2 JP6730199B2 (ja) | 2020-07-29 |
Family
ID=55631742
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016572578A Active JP6730199B2 (ja) | 2014-06-11 | 2015-06-11 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
| JP2020033434A Active JP6920491B2 (ja) | 2014-06-11 | 2020-02-28 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020033434A Active JP6920491B2 (ja) | 2014-06-11 | 2020-02-28 | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US10662426B2 (https=) |
| EP (2) | EP3155102B1 (https=) |
| JP (2) | JP6730199B2 (https=) |
| AU (2) | AU2015324564B2 (https=) |
| BR (1) | BR112016028741A2 (https=) |
| DK (1) | DK3155102T5 (https=) |
| ES (1) | ES2937642T3 (https=) |
| GB (1) | GB2528177B (https=) |
| HU (1) | HUE061007T2 (https=) |
| IL (2) | IL249191B (https=) |
| MY (2) | MY187588A (https=) |
| WO (1) | WO2016053397A2 (https=) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| JP6730199B2 (ja) * | 2014-06-11 | 2020-07-29 | デューク ユニバーシティ | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| JP7109784B2 (ja) | 2015-10-23 | 2022-08-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子編集のための進化したＣａｓ９蛋白質 |
| LT3604527T (lt) | 2016-06-02 | 2021-06-25 | Sigma-Aldrich Co., Llc | Programuojamų, dnr surišančių baltymų panaudojimas tiksliniam genomo modifikavimui pagerinti |
| US20190367930A1 (en) * | 2016-07-29 | 2019-12-05 | Danmarks Tekniske Universitet | Methods for decoupling cell growth from production of biochemicals and recombinant polypeptides |
| KR102547316B1 (ko) | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| EP3526320A1 (en) | 2016-10-14 | 2019-08-21 | President and Fellows of Harvard College | Aav delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| IL296347B2 (en) * | 2017-02-21 | 2024-03-01 | Univ Duke | Compositions and methods for robust dynamic metabolic control |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| JP2020510038A (ja) | 2017-03-09 | 2020-04-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | がんワクチン |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| EP3797160A1 (en) | 2018-05-23 | 2021-03-31 | The Broad Institute Inc. | Base editors and uses thereof |
| US10227576B1 (en) | 2018-06-13 | 2019-03-12 | Caribou Biosciences, Inc. | Engineered cascade components and cascade complexes |
| US12227776B2 (en) | 2018-06-13 | 2025-02-18 | Caribou Biosciences, Inc. | Engineered cascade components and cascade complexes |
| WO2019246488A1 (en) * | 2018-06-21 | 2019-12-26 | Duke University | Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020061385A1 (en) * | 2018-09-19 | 2020-03-26 | Conagen Inc. | Controllable protein degradation via engineering degradation tag variants in corynebacterium host cells |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| JP7657726B2 (ja) | 2019-03-19 | 2025-04-07 | ザ ブロード インスティテュート，インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| US12577572B2 (en) | 2019-10-23 | 2026-03-17 | Duke University | Compositions, systems, and methods for high level expression of recombinant protein |
| MX2022011418A (es) * | 2020-03-16 | 2022-12-15 | Univ Duke | Métodos y composiciones para el silenciamiento de génes basado en crispr tipo i-e mejorado. |
| CA3179180A1 (en) * | 2020-04-03 | 2021-12-02 | Duke University | Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control |
| US20230183757A1 (en) * | 2020-04-03 | 2023-06-15 | Duke University | Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| IL300042A (en) * | 2020-07-24 | 2023-03-01 | Univ Duke | Methods and compositions for the production of acetyl-COA derivatives |
| WO2022226507A1 (en) * | 2021-04-20 | 2022-10-27 | Duke University | Two-stage dynamic control over redox state improves cystolic expression of disulfide containing proteins in e. coli |
| CN113604416B (zh) * | 2021-09-02 | 2023-05-05 | 天津大学 | 产乙偶姻的大肠杆菌工程菌及构建方法与在全细胞催化生产乙偶姻的应用 |
| WO2023183784A2 (en) * | 2022-03-21 | 2023-09-28 | Duke University | Compositions and methods for improved malonyl-coa biosynthesis using 2-stage dynamic metabolic control |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU752105B2 (en) | 1997-12-05 | 2002-09-05 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Novel dna cloning method |
| US6355412B1 (en) | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| DE10012283A1 (de) * | 2000-03-14 | 2001-09-20 | Thomas Schweder | Wirts-Vektor-Systeme zur phosphatregulierten Überproduktion von Polypeptiden in Becillus |
| DK1412513T3 (da) * | 2001-07-30 | 2010-07-12 | Genencor Int | Phosphatbegrænset inducerbar promotor og Bacillus-ekspressiongssystem |
| RU2541785C2 (ru) | 2007-05-09 | 2015-02-20 | ЛАЛЛЕМАНД ХАНГЕРИ ЛИКВИДИТИ МЕНЕДЖМЕНТ ЭлЭлСи | ВЕКТОР ДЛЯ НОКАУТА ГЕНА АЦЕТАТКИНАЗЫ В Clostridium thermocellum, КЛЕТКА-ХОЗЯИН, ГЕНЕТИЧЕСКИ МОДИФИЦИРОВАННЫЙ МИКРООРГАНИЗМ Clostridium thermocellum, СПОСОБ ПОЛУЧЕНИЯ ТАКОГО МИКРООРГАНИЗМА И СПОСОБ ПРЕОБРАЗОВАНИЯ ЛИГНОЦЕЛЛЮЛОЗНОЙ БИОМАССЫ В ЭТАНОЛ. |
| US8163516B2 (en) * | 2008-02-08 | 2012-04-24 | Algenol Biofuels Inc. | Selection of ADH in genetically modified cyanobacteria for the production of ethanol |
| BRPI0918882A2 (pt) | 2008-07-23 | 2019-02-26 | Opx Biotechnologies Inc | métodos, sistemas e composições para tolerância aumentada de microorganismo para produção de ácido 3-hidroxipropiônico (3-hp) |
| US20110146142A1 (en) | 2008-08-18 | 2011-06-23 | Ls9, Inc. | Systems and methods for production of mixed fatty esters |
| WO2010031083A2 (en) | 2008-09-15 | 2010-03-18 | Opx Biotechnologies, Inc. | Methods, systems and compositions related to reduction of conversions of microbially produced 3-hydroxypropionic acid (3-hp) to aldehyde metabolites |
| CA2747492A1 (en) | 2008-12-22 | 2010-07-01 | Mascoma Corporation | Production of ethanol from lignocellulosic biomass |
| WO2010141468A1 (en) * | 2009-06-01 | 2010-12-09 | Way Jeffrey C | Methods and molecules for yield improvement involving metabolic engineering |
| GB2487866A (en) | 2009-09-27 | 2012-08-08 | Opx Biotechnologies Inc | Method for producing 3-Hydroxypropionic acid and other products |
| WO2011063363A2 (en) | 2009-11-20 | 2011-05-26 | Opx Biotechnologies, Inc. | Production of an organic acid and/or related chemicals |
| US8712173B2 (en) | 2010-03-12 | 2014-04-29 | Mediatek Singapore Pte. Ltd. | Methods for processing 2Nx2N block with N being positive integer greater than four under intra-prediction mode and related processing circuits thereof |
| CA2809284C (en) | 2010-08-31 | 2021-02-23 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| US9243280B2 (en) * | 2011-02-23 | 2016-01-26 | University Of Central Florida Research Foundation, Inc. | Cellular depletion of biomolecular targets |
| CN103562398A (zh) * | 2011-03-22 | 2014-02-05 | Opx生物工艺学公司 | 化学产品的微生物生产以及相关的组合物、方法和系统 |
| SG194691A1 (en) | 2011-04-29 | 2013-12-30 | Danisco Us Inc | Production of mevalonate, isoprene, and isoprenoids using genes encodingpolypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities |
| EP2562249A1 (en) | 2011-08-24 | 2013-02-27 | Helmholtz-Zentrum für Infektionsforschung GmbH | Engineered host cells and methods for the production of cis, cis-muconic acid |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| CA2905049A1 (en) | 2013-03-13 | 2014-10-02 | Trustees Of Boston University | Tunable control of protein degradation in synthetic and endogenous bacterial systems |
| EP2843043A1 (en) * | 2013-08-27 | 2015-03-04 | Evonik Industries AG | A method for producing acyl amino acids |
| PL3105313T3 (pl) * | 2014-04-02 | 2020-06-29 | The Regents Of The University Of California Office Of Technology Transfer | Moduł molekularnego zaworu upustowego dla biochemii syntetycznej który utrzymuje równowagę kofaktorów |
| US20170130210A1 (en) * | 2014-06-09 | 2017-05-11 | Massachusetts Institute Of Technology | Dynamic knockdown of central metabolism for redirecting glucose-6-phosphate fluxes |
| JP6730199B2 (ja) * | 2014-06-11 | 2020-07-29 | デューク ユニバーシティ | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
| IL296347B2 (en) * | 2017-02-21 | 2024-03-01 | Univ Duke | Compositions and methods for robust dynamic metabolic control |
| WO2019246488A1 (en) * | 2018-06-21 | 2019-12-26 | Duke University | Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control |
-
2015
- 2015-06-11 JP JP2016572578A patent/JP6730199B2/ja active Active
- 2015-06-11 ES ES15845669T patent/ES2937642T3/es active Active
- 2015-06-11 BR BR112016028741A patent/BR112016028741A2/pt not_active Application Discontinuation
- 2015-06-11 WO PCT/US2015/035306 patent/WO2016053397A2/en not_active Ceased
- 2015-06-11 GB GB1511937.3A patent/GB2528177B/en active Active
- 2015-06-11 MY MYPI2016001993A patent/MY187588A/en unknown
- 2015-06-11 MY MYPI2020004066A patent/MY203868A/en unknown
- 2015-06-11 EP EP15845669.9A patent/EP3155102B1/en active Active
- 2015-06-11 HU HUE15845669A patent/HUE061007T2/hu unknown
- 2015-06-11 EP EP22181991.5A patent/EP4148127A1/en active Pending
- 2015-06-11 US US15/317,768 patent/US10662426B2/en active Active
- 2015-06-11 AU AU2015324564A patent/AU2015324564B2/en active Active
- 2015-06-11 DK DK15845669.9T patent/DK3155102T5/da active
-
2016
- 2016-11-24 IL IL249191A patent/IL249191B/en active IP Right Grant
-
2020
- 2020-02-28 JP JP2020033434A patent/JP6920491B2/ja active Active
- 2020-03-11 IL IL273209A patent/IL273209A/en active IP Right Grant
- 2020-04-15 US US16/849,455 patent/US11098307B2/en active Active
- 2020-04-15 US US16/849,441 patent/US11142761B2/en active Active
-
2021
- 2021-06-15 AU AU2021203937A patent/AU2021203937B2/en active Active
- 2021-10-05 US US17/494,047 patent/US20220204969A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2017517268A5 (https=) | ||
| Unkles et al. | Synthetic biology tools for bioprospecting of natural products in eukaryotes | |
| Gaida et al. | Synthetic tolerance: three noncoding small RNAs, DsrA, ArcZ and RprA, acting supra-additively against acid stress | |
| Giese et al. | The AreA transcription factor in Fusarium graminearum regulates the use of some nonpreferred nitrogen sources and secondary metabolite production | |
| WO2020205605A3 (en) | OLIGONUCLEOTIDE-BASED MODULATION OF C9orf72 | |
| MY194187A (en) | Promoter system inducing expression by 3-hydroxypropionic acid and method for biological production of 3-hydroxypropionic acid using same | |
| Wang et al. | Engineering CRISPR interference system in Klebsiella pneumoniae for attenuating lactic acid synthesis | |
| MX2023009165A (es) | Promotores especificos de tricoma para la manipulacion de cannabinoides y otros compuestos en tricomas glandulares. | |
| RU2017128034A (ru) | Новый промотор и пути его применения | |
| CA2833052A1 (en) | Transcription terminator sequences | |
| WO2008140615A3 (en) | Modified messenger rna stabilizing sequences for expressing genes in bacterial cells | |
| Yoshinaga et al. | Characterization of iron-responsive promoters in the marine diatom Phaeodactylum tricornutum | |
| WO2014059275A3 (en) | Process for producing gougerotin employing streptomyces microflavus strains | |
| WO2004080386A3 (en) | Optimization of bacterial sucab expression by promoter mutagenesis for efficient l-glutamic acid production | |
| JP2006519613A5 (https=) | ||
| Subki et al. | Identification and characterisation of thiamine pyrophosphate (TPP) riboswitch in Elaeis guineensis | |
| JP2010246532A (ja) | コウジ酸の産生に必須の遺伝子を利用してコウジ酸の産生量を向上する方法 | |
| Westwood et al. | Improved recombinant protein yield using a codon deoptimized DHFR selectable marker in a CHEF1 expression plasmid | |
| ES2759724T3 (es) | Drimenol sintasas y procedimiento de producción de drimenol | |
| Wang et al. | Codon preference optimization increases prokaryotic cystatin C expression | |
| Wu et al. | Systems-level analysis of the global regulatory mechanism of CodY in Lactococcus lactis metabolism and nisin immunity modulation | |
| JP7222916B2 (ja) | ドリメノール及びそれらの混合物の製造のためのセスキテルペンシンターゼ | |
| Ben Azoun et al. | Investigating the effect of carbon source on rabies virus glycoprotein production in Pichia pastoris by a transcriptomic approach | |
| UA109141C2 (uk) | Трансгенна рослина зі збільшеним рівнем рослинного крохмалю | |
| Sun et al. | Draft genome sequence of Bacillus amyloliquefaciens XK-4-1, a plant growth-promoting endophyte with antifungal activity |